LOCUS       MG225606                9140 bp    DNA     circular  SYN 
DEFINITION  Dnmt3b (tGFP-tagged) - Mouse DNA methyltransferase 3B (Dnmt3b) transcript variant 5, (10ug)
ACCESSION   NM_001122997
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3621..4325 
                     /label="GFP"  
     misc_feature    4372..4957 
                     /label="PolyA signal"  
     misc_feature    7049..7694 
                     /label="ColE1"  
     misc_feature    5411..6205 
                     /label="Neo"  
     misc_feature    5042..5344 
                     /label="SV40 ori"  
     misc_feature    7709..8569 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3600..3606 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3600 
                     /label="Dnmt3b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGGGAGAC AGCAGACATC TGAATGAAGA AGAGGGTGCC AGCGGGTATG 
     1081 AGGAGTGCAT TATCGTTAAT GGGAACTTCA GTGACCAGTC CTCAGACACG AAGGATGCTC 
     1141 CCTCACCCCC AGTCTTGGAG GCAATCTGCA CAGAGCCAGT CTGCACACCA GAGACCAGAG 
     1201 GCCGCAGGTC AAGCTCCCGG CTGTCTAAGA GGGAGGTCTC CAGCCTTCTG AATTACACGC 
     1261 AGGACATGAC AGGAGATGGA GACAGAGATG ATGAAGTAGA TGATGGGAAT GGCTCTGATA 
     1321 TTCTAATGCC AAAGCTCACC CGTGAGACCA AGGACACCAG GACGCGCTCT GAAAGCCCGG 
     1381 CTGTCCGAAC CCGACATAGC AATGGGACCT CCAGCTTGGA GAGGCAAAGA GCCTCCCCCA 
     1441 GAATCACCCG AGGTCGGCAG GGCCGCCACC ATGTGCAGGA GTACCCTGTG GAGTTTCCGG 
     1501 CTACCAGGTC TCGGAGACGT CGAGCATCGT CTTCAGCAAG CACGCCATGG TCATCCCCTG 
     1561 CCAGCGTCGA CTTCATGGAA GAAGTGACAC CTAAGAGCGT CAGTACCCCA TCAGTTGACT 
     1621 TGAGCCAGGA TGGAGATCAG GAGGGTATGG ATACCACACA GGTGGATGCA GAGAGCAGAG 
     1681 ATGGAGACAG CACAGAGTAT CAGGATGATA AAGAGTTTGG AATAGGTGAC CTCGTGTGGG 
     1741 GAAAGATCAA GGGCTTCTCC TGGTGGCCTG CCATGGTGGT GTCCTGGAAA GCCACCTCCA 
     1801 AGCGACAGGC CATGCCCGGA ATGCGCTGGG TACAGTGGTT TGGTGATGGC AAGTTTTCTG 
     1861 AGATCTCTGC TGACAAACTG GTGGCTCTGG GGCTGTTCAG CCAGCACTTT AATCTGGCTA 
     1921 CCTTCAATAA GCTGGTTTCT TATAGGAAGG CCATGTACCA CACTCTGGAG AAAGCCAGGG 
     1981 TTCGAGCTGG CAAGACCTTC TCCAGCAGTC CTGGAGAGTC ACTGGAGGAC CAGCTGAAGC 
     2041 CCATGCTGGA GTGGGCCCAC GGTGGCTTCA AGCCTACTGG GATCGAGGGC CTCAAACCCA 
     2101 ACAAGAAGCA ACCAGTGGTT AATAAGTCGA AGGTGCGTCG TTCAGACAGT AGGAACTTAG 
     2161 AACCCAGGAG ACGCGAGAAC AAAAGTCGAA GACGCACAAC CAATGACTCT GCTGCTTCTG 
     2221 AGTCCCCCCC ACCCAAGCGC CTCAAGACAA ATAGCTATGG CGGGAAGGAC CGAGGGGAGG 
     2281 ATGAGGAGAG CCGAGAACGG ATGGCTTCTG AAGTCACCAA CAACAAGGGC AATCTGGAAG 
     2341 ACCGCTGTTT GTCCTGTGGA AAGAAGAACC CTGTGTCCTT CCACCCCCTC TTTGAGGGTG 
     2401 GGCTCTGTCA GAGTTGCCGG GATCGCTTCC TAGAGCTCTT CTACATGTAT GATGAGGACG 
     2461 GCTATCAGTC CTACTGCACC GTGTGCTGTG AGGGCCGTGA ACTGCTGCTG TGCAGTAACA 
     2521 CAAGCTGCTG CAGATGCTTC TGTGTGGAGT GTCTGGAGGT GCTGGTGGGC GCAGGCACAG 
     2581 CTGAGGATGC CAAGCTGCAG GAACCCTGGA GCTGCTATAT GTGCCTCCCT CAGCGCTGCC 
     2641 ATGGGGTCCT CCGACGCAGG AAAGATTGGA ACATGCGCCT GCAAGACTTC TTCACTACTG 
     2701 ATCCTGACCT GGAAGAATTT CAGGAGCCAC CCAAGTTGTA CCCAGCAATT CCTGCAGCCA 
     2761 AAAGGAGGCC CATTAGAGTC CTGTCTCTGT TTGATGGAAT TGCAACGGGG TACTTGGTGC 
     2821 TCAAGGAGTT GGGTATTAAA GTGGAAAAGT ACATTGCCTC CGAAGTCTGT GCAGAGTCCA 
     2881 TCGCTGTGGG AACTGTTAAG CATGAAGGCC AGATCAAATA TGTCAATGAC GTCCGGAAAA 
     2941 TCACCAAGAA AAATATTGAA GAGTGGGGCC CGTTCGACTT GGTGATTGGT GGAAGCCCAT 
     3001 GCAATGATCT CTCTAACGTC AATCCTGCCC GCAAAGGTTT ATATGAGGGC ACAGGAAGGC 
     3061 TCTTCTTCGA GTTTTACCAC TTGCTGAATT ATACCCGCCC CAAGGAGGGC GACAACCGTC 
     3121 CATTCTTCTG GATGTTCGAG AATGTTGTGG CCATGAAAGT GAATGACAAG AAAGACATCT 
     3181 CAAGATTCCT GGCATGTAAC CCAGTGATGA TCGATGCCAT CAAGGTGTCT GCTGCTCACA 
     3241 GGGCCCGGTA CTTCTGGGGT AACCTACCCG GAATGAACAG GCCCGTGATG GCTTCAAAGA 
     3301 ATGATAAGCT CGAGCTGCAG GACTGCCTGG AGTTCAGTAG GACAGCAAAG TTAAAGAAAG 
     3361 TGCAGACAAT AACCACCAAG TCGAACTCCA TCAGACAGGG CAAAAACCAG CTTTTCCCTG 
     3421 TAGTCATGAA TGGCAAGGAC GACGTTTTGT GGTGCACTGA GCTCGAAAGG ATCTTCGGCT 
     3481 TCCCTGCTCA CTACACGGAC GTGTCCAACA TGGGCCGCGG CGCCCGTCAG AAGCTGCTGG 
     3541 GCAGGTCCTG GAGTGTACCG GTCATCAGAC ACCTGTTTGC CCCCTTGAAG GACTACTTTG 
     3601 CCTGTGAAAC GCGTACGCGG CCGCTCGAGA TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA 
     3661 TGGAGATCGA GTGCCGCATC ACCGGCACCC TGAACGGCGT GGAGTTCGAG CTGGTGGGCG 
     3721 GCGGAGAGGG CACCCCCGAG CAGGGCCGCA TGACCAACAA GATGAAGAGC ACCAAAGGCG 
     3781 CCCTGACCTT CAGCCCCTAC CTGCTGAGCC ACGTGATGGG CTACGGCTTC TACCACTTCG 
     3841 GCACCTACCC CAGCGGCTAC GAGAACCCCT TCCTGCACGC CATCAACAAC GGCGGCTACA 
     3901 CCAACACCCG CATCGAGAAG TACGAGGACG GCGGCGTGCT GCACGTGAGC TTCAGCTACC 
     3961 GCTACGAGGC CGGCCGCGTG ATCGGCGACT TCAAGGTGAT GGGCACCGGC TTCCCCGAGG 
     4021 ACAGCGTGAT CTTCACCGAC AAGATCATCC GCAGCAACGC CACCGTGGAG CACCTGCACC 
     4081 CCATGGGCGA TAACGATCTG GATGGCAGCT TCACCCGCAC CTTCAGCCTG CGCGACGGCG 
     4141 GCTACTACAG CTCCGTGGTG GACAGCCACA TGCACTTCAA GAGCGCCATC CACCCCAGCA 
     4201 TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT TCCGCCGCGT GGAGGAGGAT CACAGCAACA 
     4261 CCGAGCTGGG CATCGTGGAG TACCAGCACG CCTTCAAGAC CCCGGATGCA GATGCCGGTG 
     4321 AAGAAAGAGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4381 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4441 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4501 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4561 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4621 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4681 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4741 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4801 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4861 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4921 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4981 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCTTGGCTGT GGAATGTGTG TCAGTTAGGG 
     5041 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5101 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5161 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT 
     5221 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 
     5281 GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 
     5341 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG 
     5401 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 
     5461 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 
     5521 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 
     5581 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG 
     5641 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 
     5701 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 
     5761 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 
     5821 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 
     5881 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG 
     5941 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG 
     6001 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 
     6061 GTGGCCGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 
     6121 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 
     6181 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA 
     6241 CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG 
     6301 AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG 
     6361 ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 
     6421 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 
     6481 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT 
     6541 AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA 
     6601 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA 
     6661 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT 
     6721 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT 
     6781 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT 
     6841 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 
     6901 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 
     6961 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 
     7021 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 
     7081 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 
     7141 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 
     7201 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 
     7261 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 
     7321 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 
     7381 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 
     7441 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTT 
     7501 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA 
     7561 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 
     7621 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 
     7681 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG 
     7741 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT 
     7801 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG 
     7861 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 
     7921 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 
     7981 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 
     8041 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 
     8101 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 
     8161 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 
     8221 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 
     8281 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 
     8341 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 
     8401 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 
     8461 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 
     8521 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 
     8581 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 
     8641 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 
     8701 TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA 
     8761 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT 
     8821 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT 
     8881 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC 
     8941 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT 
     9001 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT 
     9061 TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC 
     9121 AAAAATTTAA CGCGAATTTT 
//