LOCUS       MG225113                9158 bp    DNA     circular  SYN 
DEFINITION  Clcn3 (tGFP-tagged) - Mouse chloride channel 3 (Clcn3) transcript variant e, (10ug)
ACCESSION   NM_173874
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3639..4343 
                     /label="GFP"  
     misc_feature    4390..4975 
                     /label="PolyA signal"  
     misc_feature    7067..7712 
                     /label="ColE1"  
     misc_feature    5429..6223 
                     /label="Neo"  
     misc_feature    5060..5362 
                     /label="SV40 ori"  
     misc_feature    7727..8587 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3618..3624 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3618 
                     /label="Clcn3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGTCTGAG CAGCTGTTCC ATAGAGGCTA CTATAGAAAC AGCTACAACA 
     1081 GCATAACCAG CGCGAGTAGC GATGAGGAGC TCCTAGATGG AGCAGGTGCC ATTATGGACT 
     1141 TTCAGACTTC TGAAGATGAC AATTTGTTAG ACGGGGACAC AGCAGCTGGA ACTCATTATA 
     1201 CAATGACAAA TGGAGGCAGC ATTAATAGCT CTACACACTT GCTGGATCTT TTGGATGAGC 
     1261 CTATCCCAGG TGTCGGTACC TACGATGATT TCCATACTAT TGACTGGGTG CGAGAGAAGT 
     1321 GTAAGGACAG AGAAAGGCAC AGACGGATCA ACAGTAAAAA AAAAGAATCA GCATGGGAAA 
     1381 TGACAAAAAG TCTGTATGAC GCCTGGTCAG GATGGCTTGT TGTTACACTG ACGGGACTGG 
     1441 CATCAGGGGC ACTAGCTGGA TTGATAGACA TTGCTGCTGA CTGGATGACT GACCTGAAGG 
     1501 AGGGCATCTG CCTCAGTGCA TTGTGGTACA ACCATGAACA GTGTTGTTGG GGCTCTAATG 
     1561 AGACAACGTT TGAAGAGAGG GATAAATGTC CACAGTGGAA AACATGGGCA GAGTTAATCA 
     1621 TTGGCCAAGC AGAGGGCCCT GGATCTTATA TCATGAACTA CATCATGTAC ATCTTTTGGG 
     1681 CTTTGAGTTT TGCCTTTCTT GCAGTTTCTT TGGTGAAAGT ATTTGCTCCA TATGCCTGTG 
     1741 GCTCTGGAAT TCCAGAGATT AAAACTATTT TGAGTGGATT TATCATCAGA GGATACTTGG 
     1801 GAAAATGGAC TTTAATGATT AAAACTATCA CGTTAGTGCT GGCTGTGGCA TCAGGTTTGA 
     1861 GTTTAGGAAA AGAAGGTCCC CTGGTACATG TTGCCTGCTG CTGTGGAAAC ATCTTTTCCT 
     1921 ACCTCTTTCC AAAGTATAGC ACCAATGAAG CTAAAAAGAG GGAGGTGCTG TCAGCCGCCT 
     1981 CAGCTGCTGG GGTTTCTGTG GCTTTTGGTG CACCGATCGG AGGAGTTCTT TTTAGCTTGG 
     2041 AGGAGGTTAG CTATTATTTT CCTCTCAAAA CTTTATGGAG ATCATTTTTT GCTGCTTTGG 
     2101 TGGCAGCATT TGTTTTGAGA TCCATCAATC CATTTGGTAA CAGCCGTCTG GTCCTCTTTT 
     2161 ATGTGGAGTA TCATACACCA TGGTACCTTT TTGAACTGTT TCCTTTTATT CTCCTAGGGG 
     2221 TATTTGGAGG GCTTTGGGGA GCTTTTTTTA TTAGGGCAAA TATTGCCTGG TGTCGTCGAC 
     2281 GCAAGTCCAC CAAATTTGGA AAGTATCCTG TTCTCGAAGT CATTATTGTT GCAGCCATTA 
     2341 CTGCTGTGAT AGCCTTCCCC AACCCATACA CAAGGCTCAA CACCAGTGAA CTGATTAAAG 
     2401 AGCTGTTTAC AGATTGTGGG CCGTTGGAAT CCTCCTCTCT CTGTGACTAC AGAAATGACA 
     2461 TGAATGCCAG TAAAATTGTT GACGATATTC CTGACCGTCC AGCAGGCGTT GGAGTATATT 
     2521 CAGCTATCTG GCAGTTGTGC CTAGCGCTCA TATTTAAAAT AATAATGACA GTATTCACTT 
     2581 TTGGTATCAA GGTCCCGTCA GGCTTGTTTA TCCCCAGCAT GGCCATTGGA GCCATTGCAG 
     2641 GGAGAATTGT GGGGATCGCT GTGGAGCAGC TTGCCTACTA TCACCACGAC TGGTTTATCT 
     2701 TCAAGGAGTG GTGTGAGGTT GGGGCTGACT GCATCACTCC CGGGCTGTAT GCCATGGTTG 
     2761 GGGCTGCTGC GTGCTTAGGT GGTGTGACAA GAATGACTGT GTCTCTGGTG GTTATTGTTT 
     2821 TTGAACTTAC TGGAGGCTTG GAATATATTG TTCCTCTTAT GGCTGCAGTA ATGACCAGTA 
     2881 AATGGGTTGG TGATGCCTTT GGTAGGGAAG GTATTTATGA AGCACACATC CGACTAAATG 
     2941 GGTACCCTTT CTTGGATGCA AAAGAAGAAT TCACTCATAC AACCCTGGCT GCTGATGTTA 
     3001 TGAGACCTCG AAGAAGTGAC CCTCCCTTAG CTGTTTTGAC ACAGGACAAT ATGACAGTAG 
     3061 ATGACATAGA AAACATGATT AATGAAACCA GCTATAATGG CTTTCCTGTT ATAATGTCAA 
     3121 AAGAATCTCA GAGATTAGTG GGATTTGCCC TCAGAAGAGA CCTGACTATT GCAATAGAAA 
     3181 GTGCCAGAAA AAAACAAGAA GGGATTGTTG GCAGTTCTCG GGTGTGTTTT GCACAGCATA 
     3241 CTCCATCTCT TCCAGCAGAA AGTCCACGGC CATTAAAACT GAGAAGCATC CTTGACATGA 
     3301 GCCCTTTTAC AGTGACAGAC CACACCCCAA TGGAGATTGT GGTAGACATC TTTCGAAAGC 
     3361 TTGGTCTGAG GCAGTGCCTT GTAACTCACA ACGGGATTGT CTTGGGGATC ATCACAAAGA 
     3421 AGAACATTTT AGAGCATCTC GAGCAACTAA AGCAGCACGT GGAGCCCTTG ACGCCTCCTT 
     3481 GGCATTATAA CAAAAAAAGA TATCCTCCGT CATATGGCCC AGACGGCAAA CCAAGACCCC 
     3541 GCTTCAATAA TGTTCAACTG AGTCCTGTAG ATGAGGACAG AGAGGAGACA GAAGAGGAAG 
     3601 TTCGTTTGTT GAATAGCACA ATTCTTACGC GTACGCGGCC GCTCGAGATG GAGAGCGACG 
     3661 AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG 
     3721 AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCGAGCA GGGCCGCATG ACCAACAAGA 
     3781 TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT 
     3841 ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA 
     3901 TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC 
     3961 ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGATGG 
     4021 GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA 
     4081 CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGATCTGGA TGGCAGCTTC ACCCGCACCT 
     4141 TCAGCCTGCG CGACGGCGGC TACTACAGCT CCGTGGTGGA CAGCCACATG CACTTCAAGA 
     4201 GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG 
     4261 AGGAGGATCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC 
     4321 CGGATGCAGA TGCCGGTGAA GAAAGAGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4381 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4441 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4501 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4561 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4621 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4681 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4741 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4801 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4861 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4921 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4981 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC TTGGCTGTGG 
     5041 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5101 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     5161 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 
     5221 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 
     5281 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 
     5341 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 
     5401 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 
     5461 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 
     5521 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 
     5581 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 
     5641 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 
     5701 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 
     5761 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 
     5821 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 
     5881 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 
     5941 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 
     6001 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 
     6061 GACTGTGGCC GGCTGGGTGT GGCCGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 
     6121 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 
     6181 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 
     6241 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 
     6301 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 
     6361 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 
     6421 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 
     6481 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 
     6541 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 
     6601 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 
     6661 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 
     6721 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 
     6781 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 
     6841 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 
     6901 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 
     6961 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 
     7021 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 
     7081 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 
     7141 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 
     7201 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 
     7261 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 
     7321 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 
     7381 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 
     7441 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 
     7501 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 
     7561 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 
     7621 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 
     7681 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 
     7741 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 
     7801 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 
     7861 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 
     7921 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 
     7981 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 
     8041 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 
     8101 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 
     8161 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 
     8221 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 
     8281 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 
     8341 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 
     8401 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 
     8461 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 
     8521 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 
     8581 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 
     8641 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 
     8701 GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 
     8761 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 
     8821 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 
     8881 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 
     8941 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 
     9001 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 
     9061 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 
     9121 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//