LOCUS       MG224865                9323 bp    DNA     circular  SYN 
DEFINITION  Whrn (tGFP-tagged) - Mouse whirlin (Whrn) transcript variant 2, (10ug)
ACCESSION   NM_001008791
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3802..4506 
                     /label="GFP"  
     misc_feature    4553..5138 
                     /label="PolyA signal"  
     misc_feature    7230..7875 
                     /label="ColE1"  
     misc_feature    5592..6386 
                     /label="Neo"  
     misc_feature    5223..5525 
                     /label="SV40 ori"  
     misc_feature    7890..8750 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3774..3781 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3774 
                     /label="Whrn"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACGCACAG CTGGACGGCC TGTCGGTGAG CTCGTCCTCC ACCGGCTCGC 
     1081 TGGGCTCGGC GGCGGCGGCG GCGGGCGGCG GCGGGGGCGC GGGGTTGCGG CTGCTGTCTG 
     1141 CCAACGTGCG CCAGCTGCAC CAAGCGCTCA CGGCGCTGCT GAGCGAGCCC GAGCGGGAGC 
     1201 AGTTCACTCA CTGCCTCAAC GCGTACCACG CGCGCCGCAA CGTCTTCGAC CTGGTGCGCA 
     1261 CCCTGCGCGT CCTGCTGGAC AGCCCGGTCA AGCGGCGTCT GCTGCCCATG CTGCGTCTGG 
     1321 TCATCCCGCG CTCCGACCAG CTGCTCTTCG ACCAGTACAC GGCCGAGGGC CTCTACCTGC 
     1381 CAGCCACCAC CCCCTACAGG CAGCCCGCCT GGGCCGCTCC CGACGGCGCG GGGCCCGGGG 
     1441 AGGTGCGACT CGTGAGCCTG CGGCGCGCCA AGGCCCACGA GGGCTTGGGC TTCAGCATCC 
     1501 GCGGGGGCTC GGAACACGGC GTGGGCATCT ACGTGTCTCT AGTGGAGCCG GGCTCCCTGG 
     1561 CAGAGAAGGA AGGGTTGCGG GTCGGGGACC AGATTCTGCG CGTCAACGAT AAATCTCTAG 
     1621 CCCGGGTGAC CCACGCGGAG GCTGTCAAGG CTCTCAAAGG CTCCAAGAAG CTGGTGCTGT 
     1681 CTGTATACTC AGCTGGGCGT ATCCCAGGGG GCTATGTGAC CAACCACATC TACACCTGGG 
     1741 TGGACCCACA GGGTCGAAGC ACATCCCCTC CCTCCAGCCT GCCCCAGCCC CATGGCAGCA 
     1801 CCCTGAGACA GCGTGAAGAT GACCGAAGGA GTACCCTCCA CCTCCTGCAG AGTGGAGATG 
     1861 AGAAAAAGGT GAACCTGGTG TTGGGGGACG GCCGGTCCTT GGGCCTCACG ATCCGAGGTG 
     1921 GAGCAGAGTA CGGCCTTGGC ATTTACATCA CTGGTGTGGA CCCAGGCTCT GAAGCAGAGA 
     1981 GCAGCGGCCT CAAGGTTGGA GACCAGATTC TGGAGGTGAA TGGGCGGAGC TTTCTCAACA 
     2041 TCCTGCATGA TGAGGCAGTG AAGCTGCTCA AGTCATCCCG GCACCTCATC CTGACGGTGA 
     2101 AGGACGTCGG AAGGCTGCCC CACGCACGTA CCACCGTGGA CCAGACCAAG TGGATCGCCA 
     2161 GTTCCCGGAT CGGGGAAAGC GTCGCCAACT CAGCAGGGTT TCCAGGGGAC CACACAGAAG 
     2221 AAGGGACAAG CAAGCCAGGA TTTTACAAGG GCCCAGCCGG CTCCCAGGTG ACCCTGAGCA 
     2281 GCCTGGGGAA TCAGACTCGT GCACTGCTGG ACGACCAAGC CAGGCACCTG CTGACAGAGC 
     2341 AGGAGCGCGC CACCATGATG TACTACCTGG CCCAGTACCG TGGTGGCACC ATCTCTGTGG 
     2401 AGGCCATGGT CATGGCACTG TTTGAGTTGC TCAACACACA CGCCAAGTTC TCACTCCTGT 
     2461 CTGAGGTGAG AAGCATCATC TCACCCCAAG ACCTGGACCG CTTTGACCAC CTGGTGCTGA 
     2521 GGCGCGAGAT CGAGTCTATG AAGGCGAGGC AGCCCCCCGG CCCCGGGGTT GGAGACACGT 
     2581 ATTCCATGGT TTCCTACAGC GACACAGGCT CATCCACAGG CAGCCACGGC ACCTCTACAA 
     2641 CCGTCAGCTC GGCCAGGGAG CGGCTGCTGT GGCTTATAGA CCTGATGGAG AATACATTAG 
     2701 ACCTGGAAGG AACCGGTGAG ACCACCCAGG GCAGCACCAA CGCTCTCCCG GATGTGTCTG 
     2761 TGGATGATGT CAAATCCCCC TCAGAAGATC TGCCTGGTAT CAAGCCACCT CCTCCCCCAC 
     2821 CACCCCTGGC TCAAGGTCAT GACCGCTTGC TTGGCCAGCC ACGGAAGCCA GGGAGGGAGG 
     2881 ACCCCGCACC TCTGTCTTCT GCTGCCCACT CAGGCATTGT CTTCTCAGCA CCACGGAACC 
     2941 GCAGCCCGCC GCCAGGGACT GCTCCCACTC CGGGACCTTC CTCAGCACAG GACTCTCCCT 
     3001 CCTCCCCCAT TTATGCCTCC ATCTCCCATG CCAACCCCAG TTCCAGAAAG CCGCTGGACA 
     3061 CCCACCTGGC CCTGGTTAAC CAGCACCCCA TCGGCCCCTT CCCTCGAGTC CAGTCCCCAC 
     3121 CTCACCTGAA GAGCCCTCCT GCAGAGACCC CAGGAGCTGG GGCCTGCCTT CCACCACCAT 
     3181 CACCCTCTGA ACACCCTGAC GCCGTGGGTG CAAACCAGCA CTTTGTTCTG GTGGAGGTGC 
     3241 ACCGTCCGGA CAGTGAGCCT GATGTGAATG AAGTGCGGGC TCTGCCCCAG ACTCGCACAG 
     3301 CCTCCACACT CTCTCAGCTC TCAGACAGTG GGCAGACCCT GAGCGAGGAC AGTGGCGTGG 
     3361 ATGCCGGGGA GACGGAGGCC AGCACCTCAG GCCGAGGCAG ACAGACAGCA TCCGCTAAGA 
     3421 ACAAGAATGG CAAGGAGCAG CCCCGGACGG AGAGGACCGC GGAGGGTGCC AACAAACCTC 
     3481 CCGGCCTGCT GGAGCCAACG TCCACCCTGG TCCGTGTGAG GAAAAGTGCA GCCACACTGG 
     3541 GCATCGCCAT TGAGGGCGGT GCCAACACAC GCCAGCCTCT GCCCAGGATC GTCACAATTC 
     3601 AGCGAGGAGG TTCTGCCCAT AACTGTGGAC AGCTCAAGGT GGGCCACGTA ATTCTGGAAG 
     3661 TGAATGGGCA GACACTTCGG GGTAAGGAGC ACAAGGAGGC CGCCCGAATC ATCGCTGAGG 
     3721 CCTTCAAGAC CAAGGAGAGA GACTACATCG ACTTTCTGGT CACTGAGTTC AACGTGATGC 
     3781 TCAGCGGACC GACGCGTACG CGGCCGCTCG AGATGGAGAG CGACGAGAGC GGCCTGCCCG 
     3841 CCATGGAGAT CGAGTGCCGC ATCACCGGCA CCCTGAACGG CGTGGAGTTC GAGCTGGTGG 
     3901 GCGGCGGAGA GGGCACCCCC GAGCAGGGCC GCATGACCAA CAAGATGAAG AGCACCAAAG 
     3961 GCGCCCTGAC CTTCAGCCCC TACCTGCTGA GCCACGTGAT GGGCTACGGC TTCTACCACT 
     4021 TCGGCACCTA CCCCAGCGGC TACGAGAACC CCTTCCTGCA CGCCATCAAC AACGGCGGCT 
     4081 ACACCAACAC CCGCATCGAG AAGTACGAGG ACGGCGGCGT GCTGCACGTG AGCTTCAGCT 
     4141 ACCGCTACGA GGCCGGCCGC GTGATCGGCG ACTTCAAGGT GATGGGCACC GGCTTCCCCG 
     4201 AGGACAGCGT GATCTTCACC GACAAGATCA TCCGCAGCAA CGCCACCGTG GAGCACCTGC 
     4261 ACCCCATGGG CGATAACGAT CTGGATGGCA GCTTCACCCG CACCTTCAGC CTGCGCGACG 
     4321 GCGGCTACTA CAGCTCCGTG GTGGACAGCC ACATGCACTT CAAGAGCGCC ATCCACCCCA 
     4381 GCATCCTGCA GAACGGGGGC CCCATGTTCG CCTTCCGCCG CGTGGAGGAG GATCACAGCA 
     4441 ACACCGAGCT GGGCATCGTG GAGTACCAGC ACGCCTTCAA GACCCCGGAT GCAGATGCCG 
     4501 GTGAAGAAAG AGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4561 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4621 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4681 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4741 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4801 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4861 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4921 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4981 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5041 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5101 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5161 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCTTGGC TGTGGAATGT GTGTCAGTTA 
     5221 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     5281 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     5341 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 
     5401 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 
     5461 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 
     5521 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA 
     5581 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG 
     5641 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT 
     5701 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC 
     5761 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 
     5821 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG 
     5881 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA 
     5941 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA 
     6001 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT 
     6061 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 
     6121 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC 
     6181 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG 
     6241 GGTGTGGCCG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT 
     6301 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG 
     6361 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA 
     6421 TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT 
     6481 ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG 
     6541 GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT 
     6601 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 
     6661 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA 
     6721 GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA 
     6781 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 
     6841 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 
     6901 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 
     6961 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 
     7021 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 
     7081 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 
     7141 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 
     7201 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 
     7261 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 
     7321 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 
     7381 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 
     7441 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 
     7501 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 
     7561 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 
     7621 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 
     7681 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 
     7741 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 
     7801 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 
     7861 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG 
     7921 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG 
     7981 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 
     8041 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 
     8101 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG 
     8161 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG 
     8221 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT 
     8281 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 
     8341 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC 
     8401 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA 
     8461 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC 
     8521 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT 
     8581 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 
     8641 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA 
     8701 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA 
     8761 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 
     8821 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 
     8881 AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC 
     8941 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT 
     9001 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG 
     9061 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT 
     9121 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 
     9181 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 
     9241 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 
     9301 AACAAAAATT TAACGCGAAT TTT
//