LOCUS       MG224268                9524 bp    DNA     circular  SYN 
DEFINITION  Epb4.1l2 (tGFP-tagged) - Mouse erythrocyte protein band 4.1-like 2 (Epb4.1l2), (10ug)
ACCESSION   NM_013511
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4005..4709 
                     /label="GFP"  
     misc_feature    4756..5341 
                     /label="PolyA signal"  
     misc_feature    7433..8078 
                     /label="ColE1"  
     misc_feature    5795..6589 
                     /label="Neo"  
     misc_feature    5426..5728 
                     /label="SV40 ori"  
     misc_feature    8093..8953 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3984..3990 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3984 
                     /label="Epb41l2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTACTGAA GTTGGCTCTG CATCTGAAGT GAAGAAGGGC TCTGACCAGG 
     1081 CTGGAGCAGA TGCCTCCAAG GAGAAAGCTA AAGAAGTAGA AAATGAGCAG ACTCCAGTAT 
     1141 CTGAGCCAGA GGAAGAAAAG GGTTCCCAGC CAGGTCCTCC GGTTGAGAGG CAAAGCACTC 
     1201 CCCGCCTGCG GAAACGAGGG AAGGATCCGT CTGAGAACAG GGGCATCTCT CGATTCATAC 
     1261 CCCCGTGGCT TAAGAAGCAG AGGTCTTACA ATTTAGTAGT AGCCAAAGAT GGAGGAGATA 
     1321 AAAAAGAGCC TACTCAAGCT GACGTGGAAG ACCAGATCTT AGGCAAAGAG GAATCCCTTC 
     1381 CTGAAGAAGA GAGTCGGGCC AAGGGTGACG CAGAAGAAAT GGCTCAGAGG AAGCACCTGG 
     1441 AGGTTCAGGT GGAGGTCAGG GAAGCAAAAC CAGCCTTGAA AAGCAGCGTG GAGACCCAGC 
     1501 CTGCTGAAGA AGTGAGGAAG GACAAGGAGG AAACGATACA AGACACACAG GAAGAGAAAC 
     1561 TGGAAGGAGG TGCAGCCAAA AGGGAGACAA AGGAAGTACA GACAAGTGAG CTGAAGGCAG 
     1621 AGGTGGCCTC TCAGAAAGCT ACCAAGAAGA CCAAGACTGT CCTGGCCAAA GTGACCCTTC 
     1681 TAGATGGCAC GGAATACAGC TGTGACCTTG AGAAACGTGC CAAGGGACAA GTGTTGTTTG 
     1741 ACCGAGTGTG CGAGCACCTG AACCTCTTGG AGAAGGACTA CTTTGGACTT CTATTTCAGG 
     1801 ATCATCCTGA GCAGAAAAAC TGGCTAGATC CTGCAAAGGA AATAAAGAGA CAGCTGAAAA 
     1861 ATCTTCCCTG GCTGTTCACT TTCAATGTGA AATTTTATCC TCCAGATCCT TCTCAGCTGA 
     1921 CTGAAGATAT CACCAGATAC TTCTTGTGCC TTCAGCTAAG GCAGGACATC GCCTCTGGCC 
     1981 GCCTGCCCTG CTCCTTTGTG ACTCACGCCC TCTTGGGATC CTACACCTTG CAGGCCGAGC 
     2041 ATGGGGACTA TGACCCAGAA GAATATGACA GTATTGATCT TGGTGACTTC CAGTTTGCCC 
     2101 CGGCCCACAC CAAGGAGTTG GAGGAAAAGG TGTCAGAACT GCATAAAACT CACAGGGGCT 
     2161 TATCTCCAGC ACAAGCCGAC TCTCAGTTCT TAGAGAACGC AAAGAGGCTT TCCATGTACG 
     2221 GTGTTGACCT GCACCATGCC AAGGACTCTG AAGGTGTGGA CATCAAGCTG GGTGTGTGTG 
     2281 CCAATGGCCT TCTCATTTAC AAAGACAGAC TAAGGATCAA TCGTTTTGCT TGGCCGAAGA 
     2341 TTTTGAAAAT TTCCTATAAG CGCAGTAATT TCTACATTAA AGTTAGACCA GCAGAGCTGG 
     2401 AACAGTTTGA GAGTACCATT GGATTCAAAT TGCCAAACCA CCGGGCAGCT AAACGGCTAT 
     2461 GGAAGGTGTG TGTGGAACAT CACACTTTCT ACAGGCTTGT GTCTCCAGAG CAGCCACCAA 
     2521 AGACCAAGTT CTTGACCCTG GGGTCCAAAT TCCGCTACAG CGGCCGCACG CAGGCACAGA 
     2581 CCAGAGAAGC AAGTACCCTC ATAGACAGGC CGGCACCACA ATTTGAGCGT GCCTCTAGTA 
     2641 AACGTGTCTC GAGGAGCCTA GACGGAGCCC CGATTGGTGT TGTGGACCAA AGCCCTCCTG 
     2701 GAGAGGGCTC AGTGCCTGGC CCTGGAGTTA TCAGCTATAC TACCATCCAG GATGGCCGGA 
     2761 GGGACAGCAA AAGCCCAACA AAAGCTACAC CCCTGCCCGC TGAGGGAAAG AAAAATACCT 
     2821 TGAGAGTAGA CGGGGATAAT ATTTATGTCA GACATAGCAA TTTAATGTTG GAGGACCTGG 
     2881 ATAAGGCCCA GGAGGCCATT CTGAAACATC AGGCTAGCAT TAGTGAACTC AAGCGCAATT 
     2941 TCATGGCGTC CACACCTGAG CCTCGCCCCA GTGAATGGGA GAAGCGGCGG GTCACACCTC 
     3001 TGCCTTTCCA ACCACAAGCG AGTTCACATG AGACTCTGAA TGTAGTGGAG GAGAAGAAGC 
     3061 GGGCAGAGGT TGGGAAAGAC GAAAGCGTAA TCACAGAAGA AATGAATGGT AAAGAGATGT 
     3121 CACCGGGGCA TGGTCCTGGG GAGACTCGTA AGGTGGAGCC TGTGGCACAC AAAGACTCCA 
     3181 CCTCCCTGTC TTCTGAGAGC AGCAGCAGCA GCAGTGAGAG TGAGGAGGAC GTGGGAGAGT 
     3241 ACCAGCCCCA CCACCGAGTG ACCGAGGGCA CCATAAGGGA AGAGCAGGAG GAGTGTGATG 
     3301 AAGAGCTGGA GGAAGAGCCC GGCCAAGGAG CCAAGGTAGT AGAGAGGGAG GCAGCAGTGC 
     3361 CCGACGCCGT CCCAGACAGA CAAGCAGGGG CCAGTGTGCT CCCAGTAGAA ACAGAGGCCC 
     3421 AGGAACATGT AGTTGCCCAA AAGTTACCTG GAGAAAAGGG TGCACACGGA GGCACTGCTG 
     3481 AGCAGGACCC GAGAGAAGAA GCAGAGGAAG ACCCGCACAG AGTTAATGGA GAGGTGCCCC 
     3541 ATCTTGACCT TGATGGTTTG CCAGAGATTA TTTGTTGTTC AGAGCCACCA GTGGTAAAAA 
     3601 CAGAGATGGT AACAATTTCT GATGCCTCAC AAAGGACAGA AATCTCCACC AAGGAAGTCC 
     3661 CCATTGTTCA AACTGAGACC AAAACCATCA CATATGAGTC TCCACAGATT GACGGCGGGG 
     3721 CTGGTGGTGA TTCGGGCGTG TTACTGACCG CACAAACCAT CACATCTGAG TCCGCGTCAA 
     3781 CAACGACAAC CACACACATC ACCAAGACTG TGAAAGGTGG GATCTCTGAA ACACGAATTG 
     3841 AGAAACGCAT TGTGATCACA GGAGACGCAG CTCTGGATCA TGATCAGGCG CTGGCTCAGG 
     3901 CCATACGGGA AGCGAGAGAG CAGCACCCTG ATATGTCGGT CACAAGGGTG GTAGTGCACA 
     3961 AAGAAACAGA GTTGGCGGAG GAAGGAGAAG AAACGCGTAC GCGGCCGCTC GAGATGGAGA 
     4021 GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG 
     4081 GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA 
     4141 ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA 
     4201 TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC 
     4261 ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG 
     4321 TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG 
     4381 TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA 
     4441 ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC 
     4501 GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT 
     4561 TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC 
     4621 GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA 
     4681 AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4741 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4801 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4861 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4921 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4981 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5041 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5101 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5161 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5221 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5281 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5341 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG 
     5401 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5461 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5521 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 
     5581 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
     5641 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 
     5701 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 
     5761 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 
     5821 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 
     5881 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 
     5941 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 
     6001 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 
     6061 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 
     6121 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 
     6181 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 
     6241 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 
     6301 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 
     6361 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 
     6421 TTCATCGACT GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC 
     6481 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 
     6541 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 
     6601 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 
     6661 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 
     6721 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 
     6781 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 
     6841 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 
     6901 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 
     6961 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 
     7021 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 
     7081 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 
     7141 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
     7201 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     7261 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     7321 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     7381 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     7441 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     7501 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     7561 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     7621 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     7681 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     7741 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     7801 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     7861 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     7921 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     7981 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     8041 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     8101 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     8161 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     8221 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     8281 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     8341 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     8401 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     8461 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     8521 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     8581 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     8641 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     8701 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     8761 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     8821 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     8881 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     8941 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     9001 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     9061 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 
     9121 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 
     9181 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 
     9241 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 
     9301 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 
     9361 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 
     9421 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 
     9481 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//