LOCUS       MG224170                9206 bp    DNA     circular  SYN 
DEFINITION  Ect2 (tGFP-tagged) - Mouse ect2 oncogene (Ect2) transcript variant 2, (10ug)
ACCESSION   NM_001177625
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3687..4391 
                     /label="GFP"  
     misc_feature    4438..5023 
                     /label="PolyA signal"  
     misc_feature    7115..7760 
                     /label="ColE1"  
     misc_feature    5477..6271 
                     /label="Neo"  
     misc_feature    5108..5410 
                     /label="SV40 ori"  
     misc_feature    7775..8635 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3666..3672 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3666 
                     /label="Ect2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGACGAC AGCGTGTTAC CATCTCCTAC TGAGATAACT AGCTTGGCAG 
     1081 ACTCTTCAGT GTTTGATTCT AAAGTTGCTG GAATGTCTAA GGAAAACTTG TGCTTGGCGT 
     1141 CTACTTCAAA TGTTGATGAG GAAATGCCGC AGGTTGAAGC AAGAGTGATA ATGGTCCAGG 
     1201 ATGCTGGGAA ACAAGAAGAA CTTCTAAAGG CCTTAAAGGA AATGAAAGTG CCCTGTGTAA 
     1261 AGATGGACTC AATGGAAGAA TTTGAAAGTT TGGATTCCCC AGAATTTGAG AATATATTTG 
     1321 TAGTTACTGA CTTCCAGAAT TCTGTCTTCA ATGACTTATA CAAGGCTGAT TGTAGGATTG 
     1381 TTGGGCCACC AGTTATACTC AACTGTGCAC AAAGGGGAGA GCCCTTGCCA TTTTCCTGCC 
     1441 GGCCGCTGTA TTGTACGAGT ATGCTGAACC TGGTGCTCTG CTTCACTGGA TTCAGGAAGA 
     1501 AGGAGGAGCT TGTCAAATTG GTGACATTGG TTCATCATAT GGGTGGAGTT ATTCGAAAAG 
     1561 AGTGTAATTC CAAAGTAACA CATCTGGTGG CAAATTGTAC ACAAGGCGAA AAATTTAGGG 
     1621 TTGCTGTGAG CCTGGGCACT CCAATTATGA AGCCAGAATG GATTTATAAA GCGTGGGAAA 
     1681 GACGCAATGA ACAGTGTTTC TGTGCAGCAG TTGATGACTT TAGAAATGAA TTTAAAGTTC 
     1741 CTCCATTCCA AGATTGCATT TTAAGTTTCC TGGGATTTTC AGATGAAGAG AAACATAGTA 
     1801 TGGAAGAAAT GACTGAAATG CAAGGAGGTA GCTATTTACC AGTTGGGGAT GAAAGGTGCA 
     1861 CTCACCTTAT TGTTGAAGAG AATACAGTAA AGGACCTTCC ATTCGAACCT TCAAAGAAAC 
     1921 TTTTTGTTGT CAAGCAAGAG TGGTTCTGGG GAAGCATTCA GATGGACGCT CGTGCAGGAG 
     1981 AGACTATGTA TTTGTATGAA AAGGCTAATA CTCCTGAGCT CAAGAAATCG GTGTCTCTGC 
     2041 TTTCTCTAAG TACTCCAAAC AGCAACCGCA AGAGACGGAG ATTGAAAGAG ACCCTGGCTC 
     2101 AGCTCTCCAG GGAGACTGAC CTCTCTCCTT TCCCTCCCCG GAAGCGGCCC TCAGCTGAGC 
     2161 ATTCTCTTTC TATTGGCTCA CTCCTGGATA TCTCCAACAC ACCCGAGTCC AGCATCCACT 
     2221 ATGGAGAAAC ACCGAAGTCC TGTGCTAAGT CTTCTAGAAG TTCTACTCCA GTTCCTCCAA 
     2281 AGCAGTCAGC CAGGTGGCAG GTGGCGAAAG AGCTCTACCA GACTGAGAGC AATTATGTCA 
     2341 ATATACTGGC CACAATCATT CAATTATTTC AGGTGCCCTT AGAAGAAGAA GGACAGCGTG 
     2401 GTGGGCCTAT TCTTGCACCT GAGGAGATCA AGACTATTTT TGGGAGCATC CCAGATATCT 
     2461 TTGATGTGCA TATGAAGATC AAGGATGATC TTGAAGACCT TATTGCTAAC TGGGATGAGA 
     2521 GCAGAAGTAT TGGTGACATC TTTCTTAAAT ATGCAAAAGA TTTGGTAAAA ACCTACCCTC 
     2581 CGTTTGTAAA CTTCTTTGAA ATGAGCAAGG AAATGATCAT TAAATGTGAG AAACAAAAGC 
     2641 CCAGATTTCA TGCTTTCCTC AAGATAAATC AAGCTAAACC AGAATGTGGA CGACAGAGCC 
     2701 TTGTGGAACT TCTCATCCGG CCAGTGCAGA GGCTACCCAG TGTCGCTTTA CTTTTAAACG 
     2761 ATCTTAAAAA GCATACAGCT GATGAAAATC CAGACAAAAG CACTTTAGAA AAAGCTATTG 
     2821 GATCACTAAA AGAAGTAATG ACACATATCA ATGAGGATAA GAGAAAAACA GAAGCACAGA 
     2881 AGCAAATTTT TGATGTTGTT TATGAAGTAG ATGGATGCCC AGCTAATCTC TTATCTTCTC 
     2941 ATCGGAGCCT GGTGCAACGA GTGGAAACGG TTTCCCTTGG TGAGCACCCT TGCGACCGAG 
     3001 GAGAACAAGT CACTCTCTTC CTCTTCAATG ACTGCCTCGA GATAGCAAGA AAGCGGCACA 
     3061 AGGTTATTGG CACTTTTAGA AGTCCTCACG ACCGCACCCG GCCCCCCGCT TCTCTGAAGC 
     3121 ACATTCATCT CATGCCTCTT TCTCAGATTA AGAAGGTGCT GGACATCCGA GAGACAGAAG 
     3181 ATTGTCACAA TGCCTTTGCC TTGCTTGTAA GGCCACCAAC AGAACAGGCC AATGTACTGC 
     3241 TCAGCTTCCA GATGACGTCA GAGGAGCTTC CAAAGGAGAG CTGGCTGAAG ATGCTGTGCC 
     3301 GACATGTAGC CAACACCATT TGTAAGGCAG ATGCTGAGAA TCTTATGTAT GTGGCTGATC 
     3361 CAGAATCCTT TGAAGTAAAT ACAAAAGATA TGGATAGTAC ATTGAGTCGA GCATCTAGAG 
     3421 CAATAAAGAA GACTTCAAAA AAGGTTACAA GGGCATTCTC TTTTTCCAAA ACTCCCAAGA 
     3481 GAGCCCTGCG GATGGCTCTT TCATCATCCC ATAGCTCAGA GGGAAGGAGT CCTCCGAGCA 
     3541 GTGGCAAGGT TGCTGTGAGC CGTCTGTCCA GCACATCATC CTTAGCAGGT ATTCCATCTC 
     3601 CCTCCCTTGT CAGCCTCCCT TCTTTCTTTG AACGGAGAAG TCATACATTA AGTCGATCTA 
     3661 CAACTCACTT GATAACGCGT ACGCGGCCGC TCGAGATGGA GAGCGACGAG AGCGGCCTGC 
     3721 CCGCCATGGA GATCGAGTGC CGCATCACCG GCACCCTGAA CGGCGTGGAG TTCGAGCTGG 
     3781 TGGGCGGCGG AGAGGGCACC CCCGAGCAGG GCCGCATGAC CAACAAGATG AAGAGCACCA 
     3841 AAGGCGCCCT GACCTTCAGC CCCTACCTGC TGAGCCACGT GATGGGCTAC GGCTTCTACC 
     3901 ACTTCGGCAC CTACCCCAGC GGCTACGAGA ACCCCTTCCT GCACGCCATC AACAACGGCG 
     3961 GCTACACCAA CACCCGCATC GAGAAGTACG AGGACGGCGG CGTGCTGCAC GTGAGCTTCA 
     4021 GCTACCGCTA CGAGGCCGGC CGCGTGATCG GCGACTTCAA GGTGATGGGC ACCGGCTTCC 
     4081 CCGAGGACAG CGTGATCTTC ACCGACAAGA TCATCCGCAG CAACGCCACC GTGGAGCACC 
     4141 TGCACCCCAT GGGCGATAAC GATCTGGATG GCAGCTTCAC CCGCACCTTC AGCCTGCGCG 
     4201 ACGGCGGCTA CTACAGCTCC GTGGTGGACA GCCACATGCA CTTCAAGAGC GCCATCCACC 
     4261 CCAGCATCCT GCAGAACGGG GGCCCCATGT TCGCCTTCCG CCGCGTGGAG GAGGATCACA 
     4321 GCAACACCGA GCTGGGCATC GTGGAGTACC AGCACGCCTT CAAGACCCCG GATGCAGATG 
     4381 CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4441 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4501 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4561 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4621 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4681 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4741 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4801 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4861 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4921 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4981 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5041 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG 
     5101 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     5161 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5221 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC 
     5281 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT 
     5341 GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 
     5401 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 
     5461 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 
     5521 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC 
     5581 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC 
     5641 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC 
     5701 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 
     5761 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 
     5821 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC 
     5881 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT 
     5941 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC 
     6001 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC 
     6061 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 
     6121 CTGGGTGTGG CCGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG 
     6181 CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG 
     6241 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG 
     6301 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT 
     6361 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC 
     6421 GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG 
     6481 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT 
     6541 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT 
     6601 CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC 
     6661 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT 
     6721 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC 
     6781 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG 
     6841 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG 
     6901 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 
     6961 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 
     7021 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 
     7081 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 
     7141 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 
     7201 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 
     7261 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 
     7321 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 
     7381 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 
     7441 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 
     7501 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 
     7561 GCGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 
     7621 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 
     7681 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 
     7741 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 
     7801 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 
     7861 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 
     7921 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 
     7981 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 
     8041 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 
     8101 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 
     8161 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 
     8221 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 
     8281 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 
     8341 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 
     8401 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 
     8461 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 
     8521 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 
     8581 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 
     8641 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 
     8701 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 
     8761 GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA 
     8821 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC 
     8881 CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT 
     8941 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG 
     9001 GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA 
     9061 CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT 
     9121 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA 
     9181 TTTAACAAAA ATTTAACGCG AATTTT
//