LOCUS       MG224063                8033 bp    DNA     circular  SYN 
DEFINITION  Prcp (tGFP-tagged) - Mouse prolylcarboxypeptidase (angiotensinase C) (Prcp), (10ug)
ACCESSION   NM_028243
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2514..3218 
                     /label="GFP"  
     misc_feature    3265..3850 
                     /label="PolyA signal"  
     misc_feature    5942..6587 
                     /label="ColE1"  
     misc_feature    4304..5098 
                     /label="Neo"  
     misc_feature    3935..4237 
                     /label="SV40 ori"  
     misc_feature    6602..7462 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2493..2499 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2493 
                     /label="Prcp"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCTGCCGG GCTCTCCTGC TCCTGTCTTT CCTATTACTC GGGGCCGCCA 
     1081 CAACCATCCC GCCTAGGCTA AAGACCCTCG GCAGCCCGCA TTTGTCAGCC AGTCCCACAC 
     1141 CCGACCCTGC TGTAGCCCGT AAATACTCAG TTCTTTACTT CGAACAGAAG GTTGACCACT 
     1201 TTGGGTTTGC TGACATGAGA ACTTTTAAAC AGCGGTACCT AGTCGCTGAT AAACACTGGC 
     1261 AGAGAAATGG TGGATCAATA CTTTTCTACA CTGGTAACGA AGGGGACATT GTCTGGTTTT 
     1321 GCAATAATAC GGGGTTCATG TGGGATGTGG CTGAGGAGCT GAAAGCTATG CTGGTGTTCG 
     1381 CTGAGCATCG ATACTATGGG GAGTCCCTTC CCTTTGGTCA GGACTCCTTC AAGGATTCCC 
     1441 AACACTTGAA TTTCCTGACA TCAGAACAGG CTCTGGCTGA TTTTGCAGAG TTAATCAGAC 
     1501 ACTTGGAAAA AACAATCCCA GGAGCTCAAG GTCAACCTGT CATCGCCATC GGGGGCTCTT 
     1561 ATGGTGGCAT GCTTGCAGCC TGGTTCAGGA TGAAGTATCC TCACATTGTA GTTGGAGCTC 
     1621 TTGCAGCTTC TGCCCCTATC TGGCAGCTTG ATGGTATGGT TCCCTGTGGT GAGTTTATGA 
     1681 AGATTGTAAC TAACGATTTC AGGAAAAGTG GTCCTTATTG TTCAGAAAGT ATCCGCAAAT 
     1741 CCTGGAATGT CATTGACAAA CTCTCAGGTT CAGGCAGTGG TCTCCAGTCG CTCACGAACA 
     1801 TTCTTCACCT ATGTAGTCCC TTGACTTCTG AGAAAATCCC AACTTTAAAA GGATGGATAG 
     1861 CTGAGACCTG GGTGAACCTG GCCATGGTGA ACTACCCTTA CGCATGCAAC TTTTTGCAGC 
     1921 CTTTGCCTGC TTGGCCCATC AAGGAGGTGT GCCAATATTT GAAAAATCCT AATGTATCGG 
     1981 ATACAGTGCT GCTGCAGAAT ATTTTCCAAG CTCTGAGTGT ATATTATAAC TATTCAGGCC 
     2041 AAGCAGCATG CCTGAATATT TCACAAACGA CTACGAGCAG TCTGGGCTCC ATGGGCTGGA 
     2101 GCTTTCAGGC CTGCACAGAA ATGGTCATGC CCTTTTGTAC TAATGGTATT GATGACATGT 
     2161 TTGAACCCTT TCTGTGGGAC TTGGAGAAGT ATTCCAATGA CTGTTTTAAC CAGTGGGGAG 
     2221 TGAAACCACG GCCTCATTGG ATGACTACCA TGTACGGTGG CAAAAACATC AGTTCACACT 
     2281 CAAACATCAT TTTCAGCAAT GGTGAGTTAG ACCCGTGGTC AGGAGGTGGA GTAACCAGAG 
     2341 ACATCACAGA CACCCTGGTG GCTATCAACA TCCATGACGG GGCCCATCAT TTAGATCTCC 
     2401 GAGCCCACAA CGCCTTCGAT CCTTCCTCTG TGCTGTTATC TCGATTATTG GAAGTTAAAC 
     2461 ACATGAAGAA ATGGATTTTA GATTTCTATA GCAATATCCA AACGCGTACG CGGCCGCTCG 
     2521 AGATGGAGAG CGACGAGAGC GGCCTGCCCG CCATGGAGAT CGAGTGCCGC ATCACCGGCA 
     2581 CCCTGAACGG CGTGGAGTTC GAGCTGGTGG GCGGCGGAGA GGGCACCCCC GAGCAGGGCC 
     2641 GCATGACCAA CAAGATGAAG AGCACCAAAG GCGCCCTGAC CTTCAGCCCC TACCTGCTGA 
     2701 GCCACGTGAT GGGCTACGGC TTCTACCACT TCGGCACCTA CCCCAGCGGC TACGAGAACC 
     2761 CCTTCCTGCA CGCCATCAAC AACGGCGGCT ACACCAACAC CCGCATCGAG AAGTACGAGG 
     2821 ACGGCGGCGT GCTGCACGTG AGCTTCAGCT ACCGCTACGA GGCCGGCCGC GTGATCGGCG 
     2881 ACTTCAAGGT GATGGGCACC GGCTTCCCCG AGGACAGCGT GATCTTCACC GACAAGATCA 
     2941 TCCGCAGCAA CGCCACCGTG GAGCACCTGC ACCCCATGGG CGATAACGAT CTGGATGGCA 
     3001 GCTTCACCCG CACCTTCAGC CTGCGCGACG GCGGCTACTA CAGCTCCGTG GTGGACAGCC 
     3061 ACATGCACTT CAAGAGCGCC ATCCACCCCA GCATCCTGCA GAACGGGGGC CCCATGTTCG 
     3121 CCTTCCGCCG CGTGGAGGAG GATCACAGCA ACACCGAGCT GGGCATCGTG GAGTACCAGC 
     3181 ACGCCTTCAA GACCCCGGAT GCAGATGCCG GTGAAGAAAG AGTTTAAACG GCCGGCCGCG 
     3241 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3301 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3361 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3421 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3481 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3541 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3601 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3661 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3721 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     3781 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     3841 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     3901 CCAGCTTGGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     3961 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA 
     4021 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC 
     4081 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC 
     4141 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA 
     4201 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA 
     4261 GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT 
     4321 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT 
     4381 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG 
     4441 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC 
     4501 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC 
     4561 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC 
     4621 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG 
     4681 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG 
     4741 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT 
     4801 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG 
     4861 GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC 
     4921 TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCCG ACCGCTATCA GGACATAGCG 
     4981 TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG 
     5041 CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG 
     5101 TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT 
     5161 CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC 
     5221 GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC 
     5281 CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA 
     5341 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT 
     5401 CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC 
     5461 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA 
     5521 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT 
     5581 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC 
     5641 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC 
     5701 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 
     5761 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 
     5821 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 
     5881 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 
     5941 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 
     6001 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 
     6061 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 
     6121 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 
     6181 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 
     6241 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG 
     6301 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 
     6361 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 
     6421 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 
     6481 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 
     6541 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 
     6601 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC 
     6661 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC 
     6721 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT 
     6781 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG 
     6841 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA 
     6901 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA 
     6961 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT 
     7021 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG 
     7081 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA 
     7141 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC 
     7201 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT 
     7261 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC 
     7321 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA 
     7381 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA 
     7441 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA 
     7501 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 
     7561 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG 
     7621 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC 
     7681 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC 
     7741 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG 
     7801 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG 
     7861 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG 
     7921 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT 
     7981 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
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