LOCUS       MG223751                9494 bp    DNA     circular  SYN 
DEFINITION  Pam (tGFP-tagged) - Mouse peptidylglycine alpha-amidating monooxygenase (Pam), (10ug)
ACCESSION   NM_013626
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3975..4679 
                     /label="GFP"  
     misc_feature    4726..5311 
                     /label="PolyA signal"  
     misc_feature    7403..8048 
                     /label="ColE1"  
     misc_feature    5765..6559 
                     /label="Neo"  
     misc_feature    5396..5698 
                     /label="SV40 ori"  
     misc_feature    8063..8923 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3954..3960 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3954 
                     /label="Pam"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGGACGC GCCCGCAGCC GCCTGCTGCT GCTGCTGGGG CTGCTCGCCT 
     1081 TGCAGAGCAG CTGCCTGGCC TTCAGAAGCC CACTTTCTGT CTTTAAGAGG TTTAAAGAAA 
     1141 CTACCAGATC ATTTTCCAAT GAATGCCTTG GTACCACCAG ACCCATCACT CCTATTGATT 
     1201 CTTCGGATTT TACACTGGAT ATTCGCATGC CTGGGGTCAC ACCTAAAGAG TCTGACACGT 
     1261 ACTTCTGCAT GTCCATGCGT CTGCCTGTGG ATGAGGAAGC CTTCGTGATT GACTTCAAGC 
     1321 CTCGGGCCAG CATGGACACT GTCCACCATA TGCTGCTGTT TGGATGCAAC ATGCCCTCAT 
     1381 CCACTGGGAG TTACTGGTTT TGTGATGAAG GAACCTGTAC AGATAAAGCC AATATTCTAT 
     1441 ATGCCTGGGC AAGAAATGCT CCCCCCACCC GGCTCCCTAA AGGTGTTGGA TTCAGAGTTG 
     1501 GAGGGGAAAC TGGAAGCAAA TACTTCGTCC TTCAAGTTCA CTATGGGGAC ATCAGTGCTT 
     1561 TTCGAGATAA TCACAAAGAC TGTTCCGGTG TGTCCTTACA TCTCACACGT GTGCCACAGC 
     1621 CTTTGATTGC GGGCATGTAC CTTATGATGT CTGTCAACAC TGTTATACCC CCAGGAGAGA 
     1681 AAGTAGTGAA TTCTGACATT TCGTGCCATT ACAAAATGTA TCCAATGCAT GTGTTTGCCT 
     1741 ACCGAGTCCA TACTCACCAT TTAGGTAAGG TAGTGAGTGG ATACAGAGTA AGAAACGGAC 
     1801 AGTGGACGCT GATTGGACGC CAGAGCCCCC AGCTGCCACA GGCTTTTTAC CCAGTGGAAC 
     1861 ACCCAGTAGA TGTTGCTTTT GGTGATATAC TGGCAGCCAG ATGTGTGTTC ACTGGTGAGG 
     1921 GGAGGACCGA AGCCACCCAC ATCGGCGGCA CATCTAGTGA TGAAATGTGC AACTTGTACA 
     1981 TTATGTATTA CATGGAAGCC AAGCATGCAG TTTCCTTCAT GACCTGTACA CAGAATGTGG 
     2041 CTCCAGATAT GTTCAGAACT ATCCCAGAAG AGGCCAATAT TCCAATTCCT GTAAAATCCG 
     2101 ACATGGTTAT GATCCATGGG CATCACAAAG AAACAGAAAA CAAAGAAAAG AGTGCTTTAA 
     2161 TACAGCAGCC AAAGCAGGGA GAGGAGGAAG CCTTCGAGCA GGGTGATTTC TATTCACTAC 
     2221 TTTCCAAGCT GCTAGGAGAA AGGGAAGATG TTGTTCATGT GCACAAATAT AATCCTACAG 
     2281 AAAAGACAGA ATCTGGGTCA GACCTGGTAG CTGAGATTGC AAACGTAGTC CAGAAAAAGG 
     2341 ACCTTGGTCG GTCTGACGCC AGAGAGGGAG CGGAGCATGA GGAAGGGGGT AATGCTATCC 
     2401 TGGTCAGAGA CAGGATCCAC AAATTCCACA GGCTAGAGTC AACCTTGAGG CCAGCTGAGA 
     2461 GCAGAGCTCT ATCCTTCCAG CAGCCTGGTG AAGGTCCTTG GGAACCAGAA CTCGCAGGAG 
     2521 ATTTCCATGT GGAAGAAGCA CTGGAGTGGC CTGGAGTGTA CTTGTTACCA GGCCAGGTTT 
     2581 CTGGGGTGGC CCTGGATTCT AAGAATAACC TTGTGATTTT CCACAGAGGT GACCATGTTT 
     2641 GGGATGGAAA CTCTTTTGAC AGCAAGTTCG TTTACCAACA AAGAGGTCTT GGACCAATTG 
     2701 AAGAAGATAC CATCCTGGTC ATTGACCCAA ATAAAGCTGA AATACTCCAG TCCAGCGGCA 
     2761 AGAATCTGTT TTATTTACCG CATGGTTTGA GCATAGATAC AGATGGAAAT TATTGGGTCA 
     2821 CGGATGTGGC TCTCCATCAG GTGTTCAAAT TGGAACCACG TAGCAAAGAA GGCCCTCTCT 
     2881 TAGTTCTGGG AAGGAGCATG CAACCTGGCA GCGACCAAAA TCATTTCTGC CAGCCCACTG 
     2941 ATGTGGCGGT GGAGCCCAGT ACTGGAGCTG TCTTCGTGTC AGATGGTTAC TGCAACAGTC 
     3001 GGATCGTGCA GTTCTCACCA AGCGGGAAGT TCATCACTCA GTGGGGAGAA GAGTCTTCTG 
     3061 GGAGCAGTCC TAAGCCAGGG CAGTTCAGTG TTCCTCACAG TTTGGCCCTT GTGCCTCACC 
     3121 TGAACCAGTT GTGTGTGGCC GATAGGGAAA ATGGCCGGAT CCAGTGTTTC AAGACTGACA 
     3181 CCAAAGAATT TGTGAGAGAG ATTAAGCATG CGTCATTTGG AAGGAATGTC TTTGCAATCT 
     3241 CATATATACC AGGTTTCCTC TTTGCAGTAA ATGGGAAGCC TTACTTTGGA GACCAAGAAC 
     3301 CCGTGCAAGG ATTTGTGATG AACTTTTCCA GTGGGGAAAT TATAGATGTT TTCAAACCAG 
     3361 TTCGCAAGCA CTTTGACATG CCTCATGATA TTGTGGCTTC TGAAGATGGG ACTGTGTACA 
     3421 TTGGCGACGC ACACACAAAC ACCGTGTGGA AGTTCACCCT CACTGAAAGC AGGCTGGAAG 
     3481 TGGAGCATCG ATCAGTTAAA AAGGCTGGCA TTGAGGTCCC GGAAATCAAA GCCGAGGCAG 
     3541 TTGTTGAACC CAAAGTGAAG AACAAACCCA CCTCCTCAGA ATTGCAGAAG ATGCAGGAGA 
     3601 AAAAGAAACT GATCAAAGAT CCAGGCTCGG GAGTGCCTGT GGTTCTCATT ACAACCCTTC 
     3661 TGGTTATCCC TGTGGTTGTC CTGCTGGCCA TTGCCATGTT TATTCGATGG AAAAAATCAA 
     3721 GGGCCTTTGG AGATCATGAC CGAAAGCTCG AGTCGAGTTC TGGAAGAGTA CTGGGAAGAC 
     3781 TCCGAGGAAA GGGAAGCAGC GGCTTAAATC TAGGAAATTT CTTTGCAAGC CGGAAAGGCT 
     3841 ACAGCAGAAA AGGGTTCGAC CGAGTGAGCA CAGAGGGGAG TGACCAAGAG AAGGACGAAG 
     3901 ATGATGGAAG TGAGTCTGAA GAGGAGTACT CGGCTCCGCT GCCCACTCCT GCACCTTCCT 
     3961 CCACGCGTAC GCGGCCGCTC GAGATGGAGA GCGACGAGAG CGGCCTGCCC GCCATGGAGA 
     4021 TCGAGTGCCG CATCACCGGC ACCCTGAACG GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG 
     4081 AGGGCACCCC CGAGCAGGGC CGCATGACCA ACAAGATGAA GAGCACCAAA GGCGCCCTGA 
     4141 CCTTCAGCCC CTACCTGCTG AGCCACGTGA TGGGCTACGG CTTCTACCAC TTCGGCACCT 
     4201 ACCCCAGCGG CTACGAGAAC CCCTTCCTGC ACGCCATCAA CAACGGCGGC TACACCAACA 
     4261 CCCGCATCGA GAAGTACGAG GACGGCGGCG TGCTGCACGT GAGCTTCAGC TACCGCTACG 
     4321 AGGCCGGCCG CGTGATCGGC GACTTCAAGG TGATGGGCAC CGGCTTCCCC GAGGACAGCG 
     4381 TGATCTTCAC CGACAAGATC ATCCGCAGCA ACGCCACCGT GGAGCACCTG CACCCCATGG 
     4441 GCGATAACGA TCTGGATGGC AGCTTCACCC GCACCTTCAG CCTGCGCGAC GGCGGCTACT 
     4501 ACAGCTCCGT GGTGGACAGC CACATGCACT TCAAGAGCGC CATCCACCCC AGCATCCTGC 
     4561 AGAACGGGGG CCCCATGTTC GCCTTCCGCC GCGTGGAGGA GGATCACAGC AACACCGAGC 
     4621 TGGGCATCGT GGAGTACCAG CACGCCTTCA AGACCCCGGA TGCAGATGCC GGTGAAGAAA 
     4681 GAGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4741 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4801 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4861 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4921 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4981 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5041 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5101 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5161 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5221 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5281 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5341 CCTGTTGAAT TGGGTACGCG GCCAGCTTGG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA 
     5401 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5461 ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     5521 AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC 
     5581 AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG 
     5641 GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC 
     5701 TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA 
     5761 TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG 
     5821 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC 
     5881 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT 
     5941 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT 
     6001 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC 
     6061 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC 
     6121 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC 
     6181 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG 
     6241 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG 
     6301 GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT 
     6361 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCC 
     6421 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA 
     6481 TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC 
     6541 TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC 
     6601 AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT 
     6661 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA 
     6721 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA 
     6781 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT 
     6841 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT 
     6901 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC 
     6961 ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC 
     7021 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC 
     7081 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG 
     7141 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 
     7201 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 
     7261 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 
     7321 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 
     7381 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 
     7441 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 
     7501 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 
     7561 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 
     7621 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 
     7681 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 
     7741 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 
     7801 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTTTTTTTG 
     7861 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 
     7921 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 
     7981 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT 
     8041 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA 
     8101 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA 
     8161 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC 
     8221 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA 
     8281 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG 
     8341 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG 
     8401 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG 
     8461 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG 
     8521 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC 
     8581 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT 
     8641 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA 
     8701 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA 
     8761 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA 
     8821 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC 
     8881 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC 
     8941 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 
     9001 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 
     9061 CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA 
     9121 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG 
     9181 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT 
     9241 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG 
     9301 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA 
     9361 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT 
     9421 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT 
     9481 TTAACGCGAA TTTT
//