LOCUS       MG223701                9503 bp    DNA     circular  SYN 
DEFINITION  Ano3 (tGFP-tagged) - Mouse anoctamin 3 (Ano3) transcript variant 2, (10ug)
ACCESSION   NM_001128103
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3984..4688 
                     /label="GFP"  
     misc_feature    4735..5320 
                     /label="PolyA signal"  
     misc_feature    7412..8057 
                     /label="ColE1"  
     misc_feature    5774..6568 
                     /label="Neo"  
     misc_feature    5405..5707 
                     /label="SV40 ori"  
     misc_feature    8072..8932 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3963..3969 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3963 
                     /label="Ano3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCCACCAC TCAGGCTCCA TTCAGTCCTT TAAACAGCAA AAAGGTATGA 
     1081 ATATAAGCAA AAGTGAGATA ACAACTGAAG CGTCATTAAA ACCATCTAGG AGATCCCTGC 
     1141 CTTGCCTGGC CCAGAGCTAT GCTCATTCAA AAAGCTTGAG TCAATCTGCT TCACTGTTTC 
     1201 AGTCAACTGA GAGTGAATCT CAGGCTCCAA CTTCTGTAAC ATTTCTTTCT GCTGATAAGC 
     1261 CTGAACATGT TACTTCGGAG GAGAGTAGAA AGGACTCTAC CCTTAAGTGT TCCTTTGCTG 
     1321 ACCTCAGTGA CTTTTGCTTG GCTCTGGGGA AAGACAAGGA TTACCTGGAT GAATCTGAAC 
     1381 ATGCCAACTA TGATCGATCT CGTCTCCTTA ATGACTTTGT TACCAAAGAT AAGCCTGCAT 
     1441 CCAAGACAAA GCTGTCTAAG AATGACATGA GTTACATAGC ATCTAGTGGA CTCTTATTCA 
     1501 AAGATGGCAA AAAGCGAATT GATTACATCC TGGTTTATCG AAAAACAAAT ATACAATATG 
     1561 ATAAAAGAAA CACATTTGAA AAGAACCTGA GAGCAGAAGG CTTGATGTTG GAGAAGGAGC 
     1621 CAGCAATTGC CAACCCCGAC ATCATGTTTA TTAAAATTCA CATTCCATGG GACACACTGT 
     1681 GCAAGTATGC AGAGAGGCTG AACATCAGGG TGCCCTTCAG GAAGAAATGT TACTATACTG 
     1741 ACCAGAAGAA CAAATCAAAG AGCAGGGTAC AAAACTATTT CAAAAGAATC AAAAAATGGA 
     1801 TGTCCCAAAA CCCAATGGTC CTTGACAAGT CAGCGTTTCC AGAGCTGGAG GAGTCGGACT 
     1861 GCTATACTGG ACCCTTCAGC AGAGCTAGGA TTCACCACTT CATAATAAAC AATAAGGACA 
     1921 CCTTCTTCAG CAATGCTACT CGAAGCAGGA TAGTTTATCA CATGCTGGAA CGCACCAAAT 
     1981 ATGAAAATGG AATATCAAAA GTGGGTATCC GTAAGCTTAT CACTAATGGT TCATACATAG 
     2041 CAGCATTTCC CCCACATGAG GGAGCCTACA AGAGTAGCCT TCCGATCAAA ACCCATGGAC 
     2101 CTCAGAATAA CAGGCATCTG TTGTATGAGC GCTGGGCACG CTGGGGAATG TGGTACAAGC 
     2161 ATCAGCCTCT GGACTTAATC AGGATGTACT TTGGTGAGAA GATTGGCTTA TACTTCGCCT 
     2221 GGCTGGGATG GTACACCGGA ATGCTGATTC CTGCTGCAGT TGTCGGCCTG TGTGTTTTCT 
     2281 TCTATGGATT AGTCACAATG AACGAAAGCC AAGTAAGCCA AGAAATCTGT AAAGCCACTG 
     2341 AAGTCTTCAT GTGTCCTCTA TGTGACAAGA ATTGCTCACT GCAGAGACTC AACGACAGCT 
     2401 GCATCTATGC CAAGGTAACA TATTTGTTTG ATAATGGAGG GACAGTTTTC TTCGCTATTT 
     2461 TTATGGCAAT ATGGGCCACA GTCTTTCTTG AGTTTTGGAA AAGGAGAAGA AGCATTCTGA 
     2521 CTTACACTTG GGACCTTATT GAATGGGAAG AAGAGGAGGA AACACTTCGC CCCCAGTTTG 
     2581 AAGCAAAATA TTACAGGATG GAGGTAATAA ACCCCATCAC GGGGAAGCCT GAGCCTCATC 
     2641 AGCCTTCATC CGACAAAGTC ACGCGACTTC TTGTGTCTGT CTCAGGAATC TTCTTCATGA 
     2701 TCTCCTTGGT CATCACAGCA GTGTTCGCAG TTGTAGTGTA CCGTCTGGTA GTCATGGAAC 
     2761 AGTTTGCATC ATTCAAGTGG AATTTCGTCA AACAGCACTG GCAGTTTGCT ACATCTGGTG 
     2821 CTGCTGTCTG TATCAATTTC ATAATCATCA TGCTGCTGAA TCTTGCTTAT GAAAAAATTG 
     2881 CATACCTCCT CACTAACTTA GAATATCCTC GAACTGAATC AGAATGGGAA AACAGCTTTG 
     2941 CCTTGAAGAT GTTCCTTTTC CAGTTTGTCA ATTTGAACAG TTCTATCTTC TATATTGCAT 
     3001 TCTTTTTGGG AAGATTCGTA GGCCACCCAG GAAAATACAA TAAACTTTTT GAGCGGTGGA 
     3061 GACTGGAGGA ATGCCATCCT AGTGGCTGTC TGATAGACCT CTGCCTCCAG ATGGGAGTCA 
     3121 TCATGTTTTT GAAGCAAATA TGGAACAACT TCATGGAGCT GGGATATCCG TTAATCCAAA 
     3181 ACTGGTGGTC ACGACACAAA ATCAAAAGGG GAATACAAGA CGCTTCCATA CCACAATGGG 
     3241 AAAATGACTG GAACCTTCAG CCTATGAACA TCCATGGGCT GATGGACGAG TACTTAGAAA 
     3301 TGGTTTTGCA ATTTGGATTC ACTACCATCT TTGTTGCTGC TTTTCCCCTG GCCCCTCTAT 
     3361 TGGCTTTGTT AAACAATATC ATAGAAATCA GATTGGATGC ATATAAATTT GTGACCCAGT 
     3421 GGAGGAGGCC TCTGCCAGCC CGAGCCACTG ACATAGGTAT CTGGCTTGGA ATTCTTGAGG 
     3481 GAATTGGCAT ATTGGCTGTG ATCACCAATG CATTTGTAAT TGCTATTACA TCTGATTACA 
     3541 TCCCACGTTT TGTCTATGAA TACAAATATG GCCCTTGTGC AAATCATGTG AAGCAGAATG 
     3601 AAAATTGCCT AAAGGGCTAT GTCAACAATA GCTTATCCTT CTTTGACCTG AGTGAACTTG 
     3661 GTATGGGAAA ATCTGGTTAC TGCAGGTACC GAGACTATAG AGGCCCACCT TGGAGTTCCA 
     3721 AACCCTATGA ATTCACCTTA CAGTACTGGC ACATCCTGGC TGCTCGATTG GCCTTCATTA 
     3781 TCGTGTTTGA GCATCTTGTT TTTGGCATTA AGTCATTCAT TGCATACCTG ATTCCTGATA 
     3841 TACCAAAGGG CCTCCGTGAG CGCATACGGC GAGAGAAATA CTTGGTCCAG GAAATGATGT 
     3901 ATGAGGCTGA ACTGGAACAT TTACAACAAC AGAGAAGAAA AAGTGGTCAG CCTATTCACC 
     3961 ATGAATGGCC TACGCGTACG CGGCCGCTCG AGATGGAGAG CGACGAGAGC GGCCTGCCCG 
     4021 CCATGGAGAT CGAGTGCCGC ATCACCGGCA CCCTGAACGG CGTGGAGTTC GAGCTGGTGG 
     4081 GCGGCGGAGA GGGCACCCCC GAGCAGGGCC GCATGACCAA CAAGATGAAG AGCACCAAAG 
     4141 GCGCCCTGAC CTTCAGCCCC TACCTGCTGA GCCACGTGAT GGGCTACGGC TTCTACCACT 
     4201 TCGGCACCTA CCCCAGCGGC TACGAGAACC CCTTCCTGCA CGCCATCAAC AACGGCGGCT 
     4261 ACACCAACAC CCGCATCGAG AAGTACGAGG ACGGCGGCGT GCTGCACGTG AGCTTCAGCT 
     4321 ACCGCTACGA GGCCGGCCGC GTGATCGGCG ACTTCAAGGT GATGGGCACC GGCTTCCCCG 
     4381 AGGACAGCGT GATCTTCACC GACAAGATCA TCCGCAGCAA CGCCACCGTG GAGCACCTGC 
     4441 ACCCCATGGG CGATAACGAT CTGGATGGCA GCTTCACCCG CACCTTCAGC CTGCGCGACG 
     4501 GCGGCTACTA CAGCTCCGTG GTGGACAGCC ACATGCACTT CAAGAGCGCC ATCCACCCCA 
     4561 GCATCCTGCA GAACGGGGGC CCCATGTTCG CCTTCCGCCG CGTGGAGGAG GATCACAGCA 
     4621 ACACCGAGCT GGGCATCGTG GAGTACCAGC ACGCCTTCAA GACCCCGGAT GCAGATGCCG 
     4681 GTGAAGAAAG AGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4741 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4801 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4861 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4921 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4981 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5041 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5101 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5161 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5221 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5281 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5341 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCTTGGC TGTGGAATGT GTGTCAGTTA 
     5401 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     5461 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     5521 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 
     5581 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 
     5641 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 
     5701 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA 
     5761 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG 
     5821 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT 
     5881 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC 
     5941 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 
     6001 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG 
     6061 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA 
     6121 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA 
     6181 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT 
     6241 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 
     6301 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC 
     6361 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG 
     6421 GGTGTGGCCG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT 
     6481 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG 
     6541 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA 
     6601 TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT 
     6661 ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG 
     6721 GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT 
     6781 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 
     6841 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA 
     6901 GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA 
     6961 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 
     7021 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 
     7081 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 
     7141 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 
     7201 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 
     7261 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 
     7321 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 
     7381 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 
     7441 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 
     7501 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 
     7561 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 
     7621 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 
     7681 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 
     7741 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 
     7801 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 
     7861 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 
     7921 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 
     7981 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 
     8041 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG 
     8101 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG 
     8161 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 
     8221 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 
     8281 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG 
     8341 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG 
     8401 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT 
     8461 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 
     8521 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC 
     8581 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA 
     8641 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC 
     8701 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT 
     8761 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 
     8821 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA 
     8881 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA 
     8941 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 
     9001 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 
     9061 AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC 
     9121 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT 
     9181 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG 
     9241 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT 
     9301 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 
     9361 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 
     9421 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 
     9481 AACAAAAATT TAACGCGAAT TTT
//