LOCUS       MG223393                9428 bp    DNA     circular  SYN 
DEFINITION  Brdt (tGFP-tagged) - Mouse bromodomain testis-specific (Brdt) transcript variant 1, (10ug)
ACCESSION   NM_054054
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3909..4613 
                     /label="GFP"  
     misc_feature    4660..5245 
                     /label="PolyA signal"  
     misc_feature    7337..7982 
                     /label="ColE1"  
     misc_feature    5699..6493 
                     /label="Neo"  
     misc_feature    5330..5632 
                     /label="SV40 ori"  
     misc_feature    7997..8857 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3888..3894 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3888 
                     /label="Brdt"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTCTGCCA AGTCGACAAA CAGCTATTGT TAACCCCCCT CCACCAGAGT 
     1081 ACATAAACAC TAAGAAGAGT GGGCGGTTGA CGAATCAGCT GCAGTTCCTA CAGAGGGTTG 
     1141 TGCTGAAGGC CCTGTGGAAG CACGGCTTCT CTTGGCCTTT CCAACAGCCG GTGGACGCCG 
     1201 TGAAACTAAA GCTGCCTGAC TATTACACCA TCATAAAAAC CCCAATGGAT TTAAATACAA 
     1261 TTAAGAAGCG GTTGGAAAAT AAGTATTATG AGAAGGCTTC CGAATGCATA GAAGACTTCA 
     1321 ACACAATGTT CTCAAACTGC TACTTATACA ACAAGACTGG AGATGACATT GTTGTTATGG 
     1381 CCCAAGCTCT AGAGAAGTTA TTTATGCAGA AATTATCTCA GATGCCACAA GAAGAGCAAG 
     1441 TTGTGGGTGG TAAGGAGAGA ATAAAGAAAG ATATCCAACA AAAGATAGCG GTTTCCTCTG 
     1501 CTAAGGAACA AATCCCCTCC AAAGCGGCAG AGAATGTCTT TAAGCGGCAA GAGATTCCTT 
     1561 CTGGGCTTCC TGACATCTCT CTCTCGCCCT TAAACATGGC ACAAGAAGCT CCCCCGATCT 
     1621 GTGACTCGCA GTCTCTGGTC CAAATTACAA AGGGTGTGAA GAGGCGAGCA GACACAACAA 
     1681 CTCCTACCAC TTCCATAGCT AAAGCAAGTA GCGAATCTCC TCCGACACTT AGAGAAACAA 
     1741 AGCCAGTGAA CATGCCAGTA AAAGAAAATA CAGTAAAGAA CGTTTTGCCA GACTCTCAGC 
     1801 AACAACACAA AGTTTTAAAA ACAGTCAAAG TAACGGAGCA ATTAAAGCAC TGCAGCGAGA 
     1861 TTCTTAAAGA AATGCTTGCC AAGAAACATT TGCCGTATGC ATGGCCCTTC TATAATCCTG 
     1921 TGGATGCTGA TGCTTTGGGA CTCCACAACT ACTATGACGT TGTCAAAAAT CCCATGGATC 
     1981 TCGGAACGAT CAAGGGGAAA ATGGACAATC AAGAATATAA GGATGCATAT GAATTTGCTG 
     2041 CAGATGTCAG ACTGATGTTC ATGAACTGTT ACAAGTATAA TCCTCCCGAT CATGAAGTTG 
     2101 TGGCGATGGC TCGGACCCTT CAGGATGTTT TTGAACTACA TTTTGCCAAG ATTCCCGATG 
     2161 AACCTATTGA GAGTATGCAT GCATGCCACC TTACAACAAA CAGTGCACAA GCCCTCAGCA 
     2221 GAGAAAGCAG CAGCGAGGCC TCCTCGGGGG ACGCCTCTTC TGAAGACTCT GAGGATGAAC 
     2281 GAGTGCAGCA TCTTGCCAAG CTTCAGGAGC AGCTTAATGC TGTTCATCAG CAGCTGCAAG 
     2341 TTCTGTCCCA AGTTCCATTA CGTAAGCTGA AGAAAAAGAA TGAGAAGTCC AAAAGGGCGC 
     2401 CAAAAAGGAA AAAGGTTAAT AACAGAGATG AAAACCCAAG GAAAAAGCCT AAGCAAATGA 
     2461 AGCAAAAGGA GAAGGCCAAA ATCAATCAAC CAAAGAAGAA GAAGCCGCTT TTGAAATCGG 
     2521 AAGAGGAAGA CAATGCGAAG CCTATGAACT ACGATGAGAA AAGGCAGCTG AGCTTGGACA 
     2581 TAAACAAACT GCCTGGAGAC AAGCTTGGTC GAATAGTTCA CATAATACAG TCAAGGGAGC 
     2641 CTTCCCTGAG AAACTCCAAC CCGGACGAGA TCGAGATCGA CTTTGAGACC CTGAAGGCAT 
     2701 CCACACTTAG AGAACTGGAG AAGTATGTCC TGGCCTGTCT GAGGAAGAGG TCGCTGAAGC 
     2761 CCCAAGCAAA GAAGGTTGTC AGGTCTAAGG AGGAGCTCCA TTCTGAAAAA AAGCTGGAGC 
     2821 TGGAGAGGCG GTTGCTGGAC GTCAACAATC AGCTGAATTG TAGGAAACGG CAAACGAAAC 
     2881 GTCCAGCAAA AGTGGAGAAG CCGCCCCCGC CCCCGCCCCC GCCCCCGCCC CCGCCGCCTC 
     2941 CCCCGGAGCT GGCCAGTGGG TCTCGCCTGA CCGACAGCAG CAGCAGCAGC GGCAGCGGCA 
     3001 GCGGCAGCAG CAGCAGCAGC AGCGGCAGCA GCAGCAGCAG CAGCTCCTCG GGGTCCGCGA 
     3061 GCAGCAGCAG CGACTCGAGC TCCTCCGACA GCAGCGATTC TGAACCAGAA ATATTTCCTA 
     3121 AATTTACTGG AGTAAAACAG AATGATTTAC CTCCAAAAGA AAATATAAAG CAGATACAAT 
     3181 CTTCTGTGCA AGACATAACC TCTGCTGAAG CTCCCCTTGC TCAGCAGAGC ACAGCACCAT 
     3241 GCGGAGCCCC AGGGAAGCAC TCCCAGCAGA TGCTTGGTTG CCAAGTGACA CAGCATCTGC 
     3301 AGGCCACAGA GAACACTGCA TCTGTGCAGA CTCAGCCTCT TTCAGGTGAT TGCAAGCGAG 
     3361 TATTGCTTGG CCCCCCTGTG GTGCACACCT CTGCTGAGAG CCTCACAGTG CTGGAGCCAG 
     3421 AATGCCATGC TCCCGCGCAG AAGGATATAA AGATTAAGAA TGCAGACTCT TGGAAAAGTT 
     3481 TAGGGAAGCC AGTGAAAGCA TCAAGTGTAC TGAAGTCTTC AGATGAGCTT TTCAACCAGT 
     3541 TTAGAAAAGC TGCAATAGAA AAGGAGGTAA AGGCCCGGAC ACAGGAGCAG ATGCGGAAAC 
     3601 ACCTGGAGCA TAATGCGAAG GACCCAAAAG TATCTCAAGA AAATCAGAGG GAGCCTGGAA 
     3661 GTGGATTGAC TCTAGAATCT TTATCAAGTA AAGTGCAAGA TAAGTCTCTT GAAGAAGATC 
     3721 AGAGTGAACA GCAGCCACCG TCGGAAGCTC AAGACGTAAG CAAGCTCTGG CTTCTCAAAG 
     3781 ACCGGAATCT AGCGAGGGAG AAAGAGCAAG AGCGCAGGAG AAGAGAAGCA ATGGCGGGTA 
     3841 CCATTGATAT GACTCTTCAA AGTGACATTA TGACAATGTT TGAAAATAAC TTTGATACGC 
     3901 GTACGCGGCC GCTCGAGATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT 
     3961 GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA 
     4021 CCCCCGAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA 
     4081 GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA 
     4141 GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA 
     4201 TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG 
     4261 GCCGCGTGAT CGGCGACTTC AAGGTGATGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT 
     4321 TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA 
     4381 ACGATCTGGA TGGCAGCTTC ACCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT 
     4441 CCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG 
     4501 GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGGATCA CAGCAACACC GAGCTGGGCA 
     4561 TCGTGGAGTA CCAGCACGCC TTCAAGACCC CGGATGCAGA TGCCGGTGAA GAAAGAGTTT 
     4621 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4681 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4741 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4801 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4861 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4921 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4981 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5041 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5101 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5161 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5221 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5281 GAATTGGGTA CGCGGCCAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC 
     5341 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG 
     5401 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5461 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC 
     5521 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 
     5581 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 
     5641 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA 
     5701 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 
     5761 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 
     5821 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 
     5881 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 
     5941 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 
     6001 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 
     6061 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 
     6121 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 
     6181 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 
     6241 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 
     6301 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCCGACCGC 
     6361 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 
     6421 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 
     6481 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 
     6541 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT 
     6601 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 
     6661 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 
     6721 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 
     6781 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 
     6841 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 
     6901 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT 
     6961 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 
     7021 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT 
     7081 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 
     7141 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 
     7201 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 
     7261 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 
     7321 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 
     7381 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 
     7441 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 
     7501 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 
     7561 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 
     7621 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 
     7681 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 
     7741 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA 
     7801 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 
     7861 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 
     7921 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 
     7981 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 
     8041 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 
     8101 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 
     8161 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 
     8221 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 
     8281 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 
     8341 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 
     8401 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 
     8461 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 
     8521 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 
     8581 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 
     8641 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 
     8701 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 
     8761 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 
     8821 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 
     8881 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     8941 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 
     9001 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 
     9061 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 
     9121 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 
     9181 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 
     9241 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 
     9301 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 
     9361 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 
     9421 CGAATTTT
//