LOCUS       MG223248                9455 bp    DNA     circular  SYN 
DEFINITION  Tbc1d2b (tGFP-tagged) - Mouse TBC1 domain family member 2B (Tbc1d2b), (10ug)
ACCESSION   NM_194334
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3936..4640 
                     /label="GFP"  
     misc_feature    4687..5272 
                     /label="PolyA signal"  
     misc_feature    7364..8009 
                     /label="ColE1"  
     misc_feature    5726..6520 
                     /label="Neo"  
     misc_feature    5357..5659 
                     /label="SV40 ori"  
     misc_feature    8024..8884 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3915..3921 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3915 
                     /label="Tbc1d2b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGGGGCC GGGGACGGCG TGGAGGAGAG CTGCAGTGGT GGTGAGGGAG 
     1081 CGGTACCGGG AACAGGCTCG GAGGCTGGGG CGGTGGCGGG ACGGGAGCCC TCGCGGCTGT 
     1141 GCGGCTACCT GCAGAAGCTG TCGGGCAAGG GCCCGTTGCG CGGCTACCGC AGCCGCTGGT 
     1201 TCGTGTTTGA CTCGCGCCGC TGCTACCTCT ACTACTTCAA GAGTCCCCAG GACGCTCTGC 
     1261 CGCTCGGCCA CCTGGACATC GCCGACGCCT GCTTCAGCTA CCAGGGCCGC GATGAGGCTG 
     1321 CGGAGCCCGG AGCAGATCCG CCCACACACT TCCAGGTGCA CAGCGCCGGT GCAGTCACCG 
     1381 TGCTCAAGGC TCCTAATCGT GAGCTCATGA CTTACTGGCT ACAGGAGCTT CAGCAGAAAA 
     1441 GATGGGAATA TTGCAACAGC CTTGACATGA TGAAGTGGGA CAGCAGGACT TCCCCGACTC 
     1501 CAGGGGATTT TCCCAAGGGC CTTGTGGCCA GAGATACCAC AGATATAATT TCCCAGCACC 
     1561 CAAATCCTTC TGCAGAAAAA GCCAGAACTG TGCTCGCTGT GGAAGCTGCC CCTGGAGAGC 
     1621 TGGTGGGAGA CCGGGCTGCT CATCAGCCAG CTCCCGGGCA TCCGAATCCC ATCAACTTTT 
     1681 ACTCTCTGAA GCAGTGGGGA AATGAGCTCA AAAATTCAAT GTCATCATTC CGTCCTGGGA 
     1741 GAGGGCACAG TGAGAGTCGC AGGACCGTGT TTTACACCAA TGAAGAGTGG GAACTTCTAG 
     1801 ACCCGCCTCC TAAGGACCTG GAGGAGTCCC TGGTACCAGA AGAGAGGAAG AAGCCGATGC 
     1861 CTGAGGGCAG TAAAGGAGTC GCTAGTTCAG GATTCCCCTT TGAATTTGGA CGGAACCCTT 
     1921 ACAAAGGAAA GCGCCCCTTG AAAGATATAA TTGGGTCATA CAAAAACCGC CACAGCAGTA 
     1981 GTGATCCTTT GCTCGAGGGA ACGGCGACAT CATCAGGCAG CAGCGGAGGT CCCACTAAGC 
     2041 CAGTCCCCGA AATGCAGCTG CAGATTCAAA GCCAGCAGGA GGAGCTGGAG CAACTCAAGA 
     2101 AGGACCTATC CAGTCAGAAG GAGCTCATTC GGCTGCTCCA GCAGACAGTC CGGTCATCCC 
     2161 AATATGACAA GTATTTCACG AACCCCCAGA TCAGCCAGGG GGTCCCCGGG GACACACTGG 
     2221 AGCTCCTGCA CCAGAAGGAC GAGCAGATCC TGGGCCTCAG CGGCCAGCTG GAGAGGTTCG 
     2281 GCCTGGAGAA GGAAAGCCTC CAGCAGGAAG TGAGGACGCT GAAGAGCAAG GTGGGCGAGC 
     2341 TCAACGAGCG GCTCGGCATG CTGATGGAGA CCATCCAGGC CAAGGACGAG GTCATCATCA 
     2401 AGCTCAGCGC CTGTGAGGGC AGCGTGTCAT CACCCACCCT GGGGCCCAGC TCTCCTTTGG 
     2461 CCATCCCAGC CAGCAAGGAC CAGCTGGAGC TGGACAGGCT AAAAGATAGT CTGCAAGGGT 
     2521 ACAAAAGCCA AAATAAATTT CTAAACAAGG AAATTTTGGA ACTCTCGGCT CTACGAAGAA 
     2581 ATGCAGAAAG GAGAGAGAGG GATCTGATGG CTAAGTACTC TAGCCTGGAA GCCAAGCTCT 
     2641 GCCAGGTTGA AAGTAAATAC TTGATACTGC TCCAAGAAAT GAAGACACCA GTTTGCTCAG 
     2701 AGGAACAGGG CCCTGCCAGG GATGTCATAG CCCAGTTGTT GGAGGATGCT CTGCAGGTCG 
     2761 AGAGCCAAGA GCAGCCGGAG CAAGCATTCG TTAAGCCTCA TCTGGTCAGT GAGTTTGATA 
     2821 TCTACGGGTT TAGGACTGTC CCTGATGATG ATGAGGAAGA GAAGTTGGTC GCCAAGGTCC 
     2881 GAGCACTGGA CCTGAAGACT CTGTACCTCA CAGACAACCA GGAGGTTTCC ACTGGGGTCA 
     2941 AGTGGGAAAA CTATTTTGCG AGCACAATGA ACAGGGAGAT GGCATGCTCT CCGGAGCTGA 
     3001 AAAACCTGAT CCGAGCAGGC ATTCCCCACG AGCACCGCTC CAAGGTGTGG AAGTGGTGTG 
     3061 TTGACCGTCA CACCAGGAAG TTCAAGGACA GCATGGAGCC AGACTACTTC CAGACCTTGC 
     3121 TGCAGAAGGC TCTGGAGAAA CAGAACCCGG CCTCCAAGCA GATCGAGCTG GACCTGCTCC 
     3181 GGACTCTGCC CAACAACAAG CACTACTCCA GCCCCACGTC GGAGGGCATA CAGAAGCTGC 
     3241 GCAGTGTCCT GCTCGCCTTC TCCTGGCGGA ATCCCGATAT CGGCTACTGC CAAGGCCTGA 
     3301 ACAGGTTGGT GGCAGTGGCT CTCCTTTACC TGGATCAAGA AGATGCCTTC TGGTGTCTCG 
     3361 TTACCATCGT GGAAGTCTTC ATGCCCCGAG ACTATTACAC AAAGACTCTT TTAGGATCCC 
     3421 AGGTGGACCA GCGGGTGTTC AGAGACCTCC TGAGTGAGAA GCTGCCTCGA CTGCACACCC 
     3481 ATTTTGAACA GTACAAAGTG GACTACACCC TCATCACCTT CAACTGGTTT CTGGTGGTAT 
     3541 TTGTGGACAG CGTTGTCAGC GACATCCTCT TTAAAATATG GGACTCTTTC CTTTATGAGG 
     3601 GACCAAAGGT TATTTTCCGT TTTGCCCTGG CACTTTTTAA ATATAAGGAA GAGGAGATCC 
     3661 TGAAATTGCA AGATTCGATG TCCATATTCA AGTATCTGCG TTACTTCACT CGGACTATCC 
     3721 TCGATGCCAG GAAGCTGATC AGCATCTCCT TTGGGGATCT GAACCCCTTC CCCCTGCGCC 
     3781 AGATCCGGAA CCGGAGAGCC TACCACCTGG AGAAGGTCCG GCTGGAGCTG ACAGAGCTGG 
     3841 AGGCCATTCG AGAGGACTTC CTGCGTGAGC GGGACACTAG CCCTGACAAA GGCGAGCTAG 
     3901 TTAGCGATGA GGAGGAAGAC ACTACGCGTA CGCGGCCGCT CGAGATGGAG AGCGACGAGA 
     3961 GCGGCCTGCC CGCCATGGAG ATCGAGTGCC GCATCACCGG CACCCTGAAC GGCGTGGAGT 
     4021 TCGAGCTGGT GGGCGGCGGA GAGGGCACCC CCGAGCAGGG CCGCATGACC AACAAGATGA 
     4081 AGAGCACCAA AGGCGCCCTG ACCTTCAGCC CCTACCTGCT GAGCCACGTG ATGGGCTACG 
     4141 GCTTCTACCA CTTCGGCACC TACCCCAGCG GCTACGAGAA CCCCTTCCTG CACGCCATCA 
     4201 ACAACGGCGG CTACACCAAC ACCCGCATCG AGAAGTACGA GGACGGCGGC GTGCTGCACG 
     4261 TGAGCTTCAG CTACCGCTAC GAGGCCGGCC GCGTGATCGG CGACTTCAAG GTGATGGGCA 
     4321 CCGGCTTCCC CGAGGACAGC GTGATCTTCA CCGACAAGAT CATCCGCAGC AACGCCACCG 
     4381 TGGAGCACCT GCACCCCATG GGCGATAACG ATCTGGATGG CAGCTTCACC CGCACCTTCA 
     4441 GCCTGCGCGA CGGCGGCTAC TACAGCTCCG TGGTGGACAG CCACATGCAC TTCAAGAGCG 
     4501 CCATCCACCC CAGCATCCTG CAGAACGGGG GCCCCATGTT CGCCTTCCGC CGCGTGGAGG 
     4561 AGGATCACAG CAACACCGAG CTGGGCATCG TGGAGTACCA GCACGCCTTC AAGACCCCGG 
     4621 ATGCAGATGC CGGTGAAGAA AGAGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4681 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4741 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4801 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4861 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4921 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4981 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5041 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5101 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5161 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5221 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5281 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCTTG GCTGTGGAAT 
     5341 GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     5401 ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     5461 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC 
     5521 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT 
     5581 TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA 
     5641 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC 
     5701 GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC 
     5761 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA 
     5821 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT 
     5881 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG 
     5941 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA 
     6001 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 
     6061 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG 
     6121 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG 
     6181 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC 
     6241 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT 
     6301 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC 
     6361 TGTGGCCGGC TGGGTGTGGC CGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT 
     6421 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT 
     6481 CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC 
     6541 TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA 
     6601 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA 
     6661 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG 
     6721 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT 
     6781 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC 
     6841 CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT 
     6901 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG 
     6961 GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT 
     7021 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT 
     7081 TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC 
     7141 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG 
     7201 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG 
     7261 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 
     7321 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG 
     7381 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 
     7441 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG 
     7501 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT 
     7561 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 
     7621 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT 
     7681 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC 
     7741 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA 
     7801 CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 
     7861 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 
     7921 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 
     7981 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 
     8041 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 
     8101 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 
     8161 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 
     8221 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 
     8281 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 
     8341 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 
     8401 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 
     8461 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 
     8521 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 
     8581 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 
     8641 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 
     8701 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 
     8761 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 
     8821 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 
     8881 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 
     8941 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 
     9001 CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 
     9061 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 
     9121 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 
     9181 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 
     9241 AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 
     9301 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 
     9361 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 
     9421 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//