LOCUS       MG223215                9407 bp    DNA     circular  SYN 
DEFINITION  Iqsec2 (tGFP-tagged) - Mouse IQ motif and Sec7 domain 2 (Iqsec2) transcript variant 2, (10ug)
ACCESSION   NM_001005475
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3888..4592 
                     /label="GFP"  
     misc_feature    4639..5224 
                     /label="PolyA signal"  
     misc_feature    7316..7961 
                     /label="ColE1"  
     misc_feature    5678..6472 
                     /label="Neo"  
     misc_feature    5309..5611 
                     /label="SV40 ori"  
     misc_feature    7976..8836 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3867..3873 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3867 
                     /label="Iqsec2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAACCCCCC TGGAGGAGCA GCAGGTCCAC AGCGTCTCAT ACTCTACACC 
     1081 AGTATTGCTG TCCTACTCAG GTCCTTGACT CCATGAAGCT TACCCCCTCA GGCAGGCTGG 
     1141 CAGAGAGCAG TGTGGAGGGT GATGCCCCAG GCAGTGACCT GAGCACAGCG GTTGATAGTC 
     1201 CTGGGAGCCA ACCCCCCTAC CGGCTGAGCC AACTACCCCC CACCAGCAGC CACATGGGGG 
     1261 GCCCCCCTGC CGGGGTGGGC CTTCCTTGGG CTCAGAGGGC ACGCCTGCAG CCAGCCAGTG 
     1321 TCGCCCTGAG GAAGCAGGAG GAGGAGGAGA TCAAGCGCTC CAAGGCCCTG TCAGACAGCT 
     1381 ATGAACTCTC CACAGACCTG CAGGACAAGA AGGTGGAAAT GCTGGAGAGG AAGTATGGGG 
     1441 GCTCCTTCCT GAGCCGCAGG GCTGCCAGGA CCATCCAGAC AGCCTTTCGA CAGTACCGTA 
     1501 TGAATAAGAA CTTTGAGCGG CTGCGCAGCT CAGCTTCAGA GAGCCGCATG TCCCGCCGCA 
     1561 TCATCTTGTC CAACATGCGG ATGCAGTTCT CCTTTGAGGA GTACGAGAAA GCACAGAACC 
     1621 CTGCATACTT CGAGGGCAAG CCTGCCTCAT TGGATGAGGG TGCCATGGCT GGCGCCCGGA 
     1681 GCCACCGGCT TGAACGGGGC CTCCCTTATG GAGGCTCCTG TGGTGGGGGC ATCGATGGTG 
     1741 GTGGAAGTTC AGTCACCACA TCTGGAGAGT TTTCTAATGA CATCACAGAG CTCGAGGACT 
     1801 CCTTCTCCAA ACAGGTAAAG TCTCTGGCTG AATCCATTGA TGAGGCCCTG AACTGCCACC 
     1861 CATCAGGGCC TATGTCTGAG GAGCCAGGGT CAGCCCAACT GGAGAAGCGG GAATCAAAGG 
     1921 AACAGCAAGA AGACAGCTCA GCCACATCCT TCAGTGACCT CCCCCTGTAC TTGGATGACC 
     1981 CAGTTCCTCC TCCATCACCT GAGCGACTGC CCAGCACAGA GCCCCCACCC CAAGGCCGCC 
     2041 CTGAGTTCTG GGCACCAGCT CCCCTCCCAC CAGTTCCTCC ACCAATGCCA CCAGGGACCC 
     2101 GGGAAGATGG TAGCCGTGAG GAAGGCACTC GAAGGGGTCC TGGGTGCTTG GAGTGCCGGG 
     2161 ATTTCCGGCT CCGGGCTGCA CACCTCCCCC TCCTCACCAT TGAGCCTCCC AGTGACAGTT 
     2221 CTGTAGACCT GAGTGACCGC TCAGATCGCG GCTCTGTCCA CCGCCAGCTG GTGTATGAGG 
     2281 CTGATGGCTG CAGCCCCCAT GGGACCCTGA AGCACAAGGG GCCACCAGGC AGGGCCCCAA 
     2341 TCCCACACCG CCACTACCCT GCCCCTGAGG GTCCAGCTCC AGCCCCACCA GGGCCCCTGC 
     2401 CACCAGCCCC CAATAGTGGT ACTGGGCCCA GTGGTGTGGC TGGAGGTCGG AGGTTGGGGA 
     2461 AGTGTGAGGC AGCAGGTGAG AACTCTGATG GTGGAGACAA TGAGAGTCTG GAGAGCTCCA 
     2521 GCAACTCTAA TGAGACCATC AACTGCAGCT CTGGCTCCTC CTCTCGGGAT AGCCTGAGGG 
     2581 AACCGCCAGC CACTGGCCTG TGCAAGCAGA CTTACCAGCG GGAGACAAGG CACAGCTGGG 
     2641 ACTCGCCAGC CTTCAACAAT GATGTGGTAC AAAGGCGACA TTACAGAATT GGCCTCAACC 
     2701 TCTTCAATAA GAAGCCAGAG AAGGGTATCC AGTATTTGAT CGAACGAGGC TTCCTGTCTG 
     2761 ACACTCCTGT TGGGGTGGCT CACTTCATCC TGGAGAGGAA AGGCCTGAGC CGGCAGATGA 
     2821 TAGGGGAATT CCTGGGGAAT CGGCAGAAGC AGTTCAACAG AGATGTGTTG GACTGTGTGG 
     2881 TGGATGAGAT GGACTTCTCC TCTATGGATT TGGACGATGC ACTCAGGAAG TTCCAATCCC 
     2941 ATATCCGAGT TCAGGGGGAG GCCCAGAAAG TGGAGCGACT CATTGAAGCT TTCAGCCAGC 
     3001 GGTACTGTGT CTGTAACCCT GCCCTCGTAC GCCAGTTCCG GAACCCAGAC ACCATCTTCA 
     3061 TCCTTGCTTT TGCCATCATC CTCCTCAATA CCGATATGTA CAGCCCCAGT GTCAAGGCTG 
     3121 AACGCAAGAT GAAGCTGGAT GACTTCATTA AGAACCTGAG AGGAGTTGAC AATGGTGAAG 
     3181 ACATCCCCCG CGACCTTCTA GTAGGCATCT ACCAGCGCAT CCAAGGTCGT GAACTGAGGA 
     3241 CCAATGATGA CCATGTGTCC CAGGTGCAGG CTGTGGAGCG CATGATTGTT GGCAAGAAAC 
     3301 CGGTCCTGTC GCTCCCTCAC CGTCGACTGG TTTGCTGCTG CCAGCTCTAT GAGGTGCCAG 
     3361 ATCCAAACCG TCCCCAAAGG CTGGGGCTGC ATCAGCGGGA GGTCTTCCTC TTCAATGACC 
     3421 TCCTTGTGGT CACCAAAATT TTCCAGAAGA AGAAGATCTT GGTGACATAC AGCTTCCGTC 
     3481 AGTCCTTTCC CCTGGTGGAA ATGCATATGC AGCTCTTCCA GAATTCATAT TATCAGTTTG 
     3541 GGATCAAGTT ACTGTCTGCA GTACCTGGAG GGGAACGAAA AGTCCTCATC ATCTTTAATG 
     3601 CTCCTAGCCT CCAAGACAGG CTACGCTTTA CCTCTGACCT GCGGGAGTCC ATTGCTGAGG 
     3661 TGCAGGAGAT GGAGAAGTAT CGTGTGGAAT CGGAGCTGGA AAAGCAGAAA GGTATGATGC 
     3721 GGCCTAATGC CTCACAGCCT GGAGGAGCCA AGGACTCAGT GAATGGGACT CTGGCCCGCA 
     3781 GTAGTCTGGA GGACACTTAT GGGGCAGGCG ATGGGCTCAA GCGGGGTGCA CTCAGTAGTT 
     3841 CCCTGCGAGA CCTCTCTGAT GCAGGGGTCT GTTATACGCG TACGCGGCCG CTCGAGATGG 
     3901 AGAGCGACGA GAGCGGCCTG CCCGCCATGG AGATCGAGTG CCGCATCACC GGCACCCTGA 
     3961 ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG GAGAGGGCAC CCCCGAGCAG GGCCGCATGA 
     4021 CCAACAAGAT GAAGAGCACC AAAGGCGCCC TGACCTTCAG CCCCTACCTG CTGAGCCACG 
     4081 TGATGGGCTA CGGCTTCTAC CACTTCGGCA CCTACCCCAG CGGCTACGAG AACCCCTTCC 
     4141 TGCACGCCAT CAACAACGGC GGCTACACCA ACACCCGCAT CGAGAAGTAC GAGGACGGCG 
     4201 GCGTGCTGCA CGTGAGCTTC AGCTACCGCT ACGAGGCCGG CCGCGTGATC GGCGACTTCA 
     4261 AGGTGATGGG CACCGGCTTC CCCGAGGACA GCGTGATCTT CACCGACAAG ATCATCCGCA 
     4321 GCAACGCCAC CGTGGAGCAC CTGCACCCCA TGGGCGATAA CGATCTGGAT GGCAGCTTCA 
     4381 CCCGCACCTT CAGCCTGCGC GACGGCGGCT ACTACAGCTC CGTGGTGGAC AGCCACATGC 
     4441 ACTTCAAGAG CGCCATCCAC CCCAGCATCC TGCAGAACGG GGGCCCCATG TTCGCCTTCC 
     4501 GCCGCGTGGA GGAGGATCAC AGCAACACCG AGCTGGGCAT CGTGGAGTAC CAGCACGCCT 
     4561 TCAAGACCCC GGATGCAGAT GCCGGTGAAG AAAGAGTTTA AACGGCCGGC CGCGGTCATA 
     4621 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4681 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4741 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4801 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4861 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4921 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4981 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5041 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5101 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5161 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5221 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCT 
     5281 TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     5341 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 
     5401 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 
     5461 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 
     5521 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 
     5581 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT 
     5641 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA 
     5701 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 
     5761 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 
     5821 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA 
     5881 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 
     5941 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA 
     6001 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 
     6061 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 
     6121 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 
     6181 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC 
     6241 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT 
     6301 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCCGACCGCT ATCAGGACAT AGCGTTGGCT 
     6361 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 
     6421 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC 
     6481 TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG 
     6541 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG 
     6601 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT 
     6661 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA 
     6721 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC 
     6781 ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 
     6841 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 
     6901 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 
     6961 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 
     7021 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 
     7081 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 
     7141 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 
     7201 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 
     7261 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 
     7321 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 
     7381 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 
     7441 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 
     7501 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 
     7561 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 
     7621 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 
     7681 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 
     7741 GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 
     7801 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 
     7861 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 
     7921 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 
     7981 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 
     8041 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 
     8101 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 
     8161 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 
     8221 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 
     8281 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 
     8341 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 
     8401 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 
     8461 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 
     8521 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 
     8581 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 
     8641 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 
     8701 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 
     8761 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 
     8821 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 
     8881 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 
     8941 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA 
     9001 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC 
     9061 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 
     9121 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 
     9181 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 
     9241 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA 
     9301 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT 
     9361 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//