LOCUS       MG222724                9314 bp    DNA     circular  SYN 
DEFINITION  Wwp1 (tGFP-tagged) - Mouse WW domain containing E3 ubiquitin protein ligase 1 (Wwp1), (10ug)
ACCESSION   NM_177327
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3795..4499 
                     /label="GFP"  
     misc_feature    4546..5131 
                     /label="PolyA signal"  
     misc_feature    7223..7868 
                     /label="ColE1"  
     misc_feature    5585..6379 
                     /label="Neo"  
     misc_feature    5216..5518 
                     /label="SV40 ori"  
     misc_feature    7883..8743 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3774..3780 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3774 
                     /label="Wwp1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCACTGCT TCACCAAGAT CTGACACTAG TGATATCCAC AGTGGAAGGT 
     1081 TACAGTTAAA GGTAACGGTT TCTAGTGCCA AACTAAAAAG AAAAAAGAAC TGGTTTGGAA 
     1141 CAGCAATATA TACTGAAGTC ATTGTAGATG GAGAAGTTAA GAAAACAGCA AAATCCAGTA 
     1201 GTTCCTCTAA TCCAAAATGG GACGAGCAGC TGATTGTAAA TGTGACCCCA CAGACCACCT 
     1261 TGGAGTTCCG AGTTTGGAGT CACCACACTT TAAAGGCAGA TGCTCTATTA GGAAAAGCAA 
     1321 CAGTGGACTT GAAACAAGTG CTGTTAACAC ACAACAGAAA ATTAGAAAAA GTGAAAGAAC 
     1381 AATTAAAACT TTCCTTGGAA AACAAGAATG GAATAGTGCA AACTGGGGAA CTGACAGTTG 
     1441 TCCTTGATGG ATTAGTGATT GAGCAAGAGC CTGTAACAAA CCGCAGCTCC TCGCCACCCA 
     1501 TAGAAATTCA GCAAAATGGA GATGCCTTAC ATGAGAATGG AGACCCTGCA ACAAGGACAA 
     1561 CTCCCAGGTT GCCTGTTGAA GGTACTATTG GAATTGATAA TCATGTATCA ACCAATACTG 
     1621 TTGTTCCCAA CTCCTGCTGT TCACATGTAG TTAATGGAGA AAACACACCT TCATCCCCGT 
     1681 CTCAGGTTGC TGCCAGACCC AAAAATGCTC CAGCTCCAAA GCCAGTCACA TCTGCGCCCA 
     1741 CCAGTGACAC GGTTAATGGA GAATCATCAT CTGTCCTAGC TGATAATACT TCAACCATGG 
     1801 GTACTTTACT ACCATCTGAA GATACCACCT CGACTTCCAA TTGCACTAGC ACCACCACCC 
     1861 AAGAGCCTCC TGTACAAGAA CCCCCAGCAT CCTCAGAACA CAGTGAATGT ATTCCCTCTG 
     1921 CCAGTGCGGA AGTGGGACCA GAAGCTCGGA GCCTCATAGA CCCTGACTCT GATTCTAGAA 
     1981 ATAATTCTGT GTTTGACAAA GTAAGGCAGC CAGAAGGGTG TGTAGAACCT CTGCGGCCAC 
     2041 AGTCTGGAAA CACCAACACA GAAGCTTTGC CATCAGGGTG GGAACAGAGA AAAGATCCTC 
     2101 ATGGTAGAAC CTATTATGTG GATCATAATA CTCGAACTAC TACATGGGAG AGACCACAGC 
     2161 CTTTGCCTCC AGGTTGGGAA AGAAGAGTTG ATGATCGTGG AAGAGTTTAT TATGTGGATC 
     2221 ATAACACCAG AACAACAACC TGGCAGCGGC CTACTATGGA ATCTGTTCGA AATTTTGAAC 
     2281 AGTGGCAGTC TCAGCGGAAT CAATTGCAGG GAGCTATGCA ACAATTCAAC CAACGATACC 
     2341 TCTATTCGGC TTCCATGTTA GCTGCAGAAA ATGACCCCTA TGGACCCTTA CCACCGGGCT 
     2401 GGGAAAAAAG AGTGGATTCA ACAGACAGAG TTTACTTTGT GAATCACAAT ACAAAAACAA 
     2461 CCCAGTGGGA AGATCCAAGA ACTCAAGGCT TACCAAATGA AGAACCCCTG CCCGAAGGCT 
     2521 GGGAAATCAG GTATACTCGG GAAGGTGTTA GGTACTTTGT TGATCATAAC ACGAGAACAA 
     2581 CAACATTCAA AGACCCTCGC AATGGGAAGT CATCAGTAAC TAAAGGTGGT CCACAGATTG 
     2641 CTTATGAACG CAGCTTTAGG TGGAAGCTTG CTCACTTCCG TTACTTGTGC CAGTCTAATG 
     2701 CACTACCGAG TCATGTAAAG ATCAATGTGT CCCGACAGAC ATTATTTGAA GATTCCTTCC 
     2761 AACAGATTAT GGCATTAAAA CCATATGACT TGAGGAGGCG ATTATATGTA ATATTTAGAG 
     2821 GAGAAGAAGG TCTTGATTAT GGTGGCCTTG CAAGAGAATG GTTTTTCTTG CTTTCCCATG 
     2881 AAGTTTTGAA CCCAATGTAT TGCTTATTTG AGTATGCAGG CAAGAATAAC TATTGTTTAC 
     2941 AGATAAATCC AGCATCAACT ATAAATCCTG ACCATCTTTC ATACTTCTGT TTTATTGGTC 
     3001 GCTTTATTGC AATGGCACTG TTCCATGGAA AGTTTATTGA TACTGGTTTC TCTTTACCAT 
     3061 TCTACAAGCG TATGCTAAGT AAAAAACTTA CTATTAAGGA TTTGGAATCT ATTGATACTG 
     3121 AATTTTACAA CTCTCTAATC TGGATAAGAG ATAACAATAT TGAAGAATGT GGCTTAGAAA 
     3181 TGTACTTTTC TGTAGACATG GAGATTCTGG GAAAAGTTAC TTCACATGAT TTAAAGTTGG 
     3241 GAGGTTCCAA TATCCTGGTG ACGGAAGAAA ACAAAGATGA ATATATTGGT TTAATGACAG 
     3301 AATGGCGTTT TTCTCGAGGA GTACAAGAAC AGACCAAAGC TTTCCTTGAT GGGTTTAATG 
     3361 AAGTTGTTCC CCTTCAGTGG CTACAGTACT TTGATGAAAA AGAATTGGAG GTTATGCTGT 
     3421 GTGGCATGCA AGAGGTTGAC TTGGCAGACT GGCAGAGAAA TACTGTTTAT CGACATTATA 
     3481 CAAGAAACAG CAAGCAAATT ATTTGGTTTT GGCAGTTTGT GAAAGAGACT GATAATGAAG 
     3541 TACGAATGCG ACTCTTGCAG TTTGTGACTG GAACCTGTCG CTTGCCTCTA GGAGGATTTG 
     3601 CTGAGCTCAT GGGAAGTAAT GGGCCTCAAA AGTTCTGTAT TGAAAAAGTT GGCAAAGACA 
     3661 CCTGGTTACC AAGAAGCCAT ACATGTTTTA ATCGCTTGGA TCTACCACCA TATAAGAGTT 
     3721 ATGAACAACT AAAGGAAAAA CTTCTTTTTG CAATAGAAGA GACAGAGGGA TTTGGACAAG 
     3781 AAACGCGTAC GCGGCCGCTC GAGATGGAGA GCGACGAGAG CGGCCTGCCC GCCATGGAGA 
     3841 TCGAGTGCCG CATCACCGGC ACCCTGAACG GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG 
     3901 AGGGCACCCC CGAGCAGGGC CGCATGACCA ACAAGATGAA GAGCACCAAA GGCGCCCTGA 
     3961 CCTTCAGCCC CTACCTGCTG AGCCACGTGA TGGGCTACGG CTTCTACCAC TTCGGCACCT 
     4021 ACCCCAGCGG CTACGAGAAC CCCTTCCTGC ACGCCATCAA CAACGGCGGC TACACCAACA 
     4081 CCCGCATCGA GAAGTACGAG GACGGCGGCG TGCTGCACGT GAGCTTCAGC TACCGCTACG 
     4141 AGGCCGGCCG CGTGATCGGC GACTTCAAGG TGATGGGCAC CGGCTTCCCC GAGGACAGCG 
     4201 TGATCTTCAC CGACAAGATC ATCCGCAGCA ACGCCACCGT GGAGCACCTG CACCCCATGG 
     4261 GCGATAACGA TCTGGATGGC AGCTTCACCC GCACCTTCAG CCTGCGCGAC GGCGGCTACT 
     4321 ACAGCTCCGT GGTGGACAGC CACATGCACT TCAAGAGCGC CATCCACCCC AGCATCCTGC 
     4381 AGAACGGGGG CCCCATGTTC GCCTTCCGCC GCGTGGAGGA GGATCACAGC AACACCGAGC 
     4441 TGGGCATCGT GGAGTACCAG CACGCCTTCA AGACCCCGGA TGCAGATGCC GGTGAAGAAA 
     4501 GAGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4561 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4621 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4681 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4741 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4801 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4861 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4921 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4981 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5041 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5101 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5161 CCTGTTGAAT TGGGTACGCG GCCAGCTTGG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA 
     5221 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5281 ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     5341 AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC 
     5401 AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG 
     5461 GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC 
     5521 TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA 
     5581 TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG 
     5641 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC 
     5701 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT 
     5761 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT 
     5821 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC 
     5881 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC 
     5941 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC 
     6001 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG 
     6061 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG 
     6121 GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT 
     6181 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCC 
     6241 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA 
     6301 TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC 
     6361 TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC 
     6421 AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT 
     6481 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA 
     6541 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA 
     6601 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT 
     6661 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT 
     6721 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC 
     6781 ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC 
     6841 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC 
     6901 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG 
     6961 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 
     7021 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 
     7081 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 
     7141 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 
     7201 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 
     7261 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 
     7321 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 
     7381 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 
     7441 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 
     7501 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 
     7561 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 
     7621 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTTTTTTTG 
     7681 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 
     7741 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 
     7801 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT 
     7861 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA 
     7921 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA 
     7981 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC 
     8041 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA 
     8101 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG 
     8161 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG 
     8221 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG 
     8281 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG 
     8341 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC 
     8401 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT 
     8461 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA 
     8521 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA 
     8581 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA 
     8641 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC 
     8701 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC 
     8761 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 
     8821 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 
     8881 CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA 
     8941 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG 
     9001 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT 
     9061 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG 
     9121 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA 
     9181 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT 
     9241 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT 
     9301 TTAACGCGAA TTTT
//