LOCUS       MG222602                9116 bp    DNA     circular  SYN 
DEFINITION  Dlg2 (tGFP-tagged) - Mouse discs large homolog 2 (Drosophila) (Dlg2), (10ug)
ACCESSION   NM_011807
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3597..4301 
                     /label="GFP"  
     misc_feature    4348..4933 
                     /label="PolyA signal"  
     misc_feature    7025..7670 
                     /label="ColE1"  
     misc_feature    5387..6181 
                     /label="Neo"  
     misc_feature    5018..5320 
                     /label="SV40 ori"  
     misc_feature    7685..8545 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3576..3582 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3576 
                     /label="Dlg2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCTTTGCA TGTTATTGTG CACTCCGGAC TAACGTAAAG AAGTATCGAT 
     1081 ACCAAGATGA GGACGGTCCA CATGATCATT CCTTACCTCG GCTAACTCAT GAAGTAAGAG 
     1141 GGCCAGAACT TGTGCATGTG TCGGAAAAGA ACCTCTCTCA AATAGAAAAT GTGCACGGAT 
     1201 ATGTCTTACA GTCTCACATC TCTCCTCTGA AGGCTAGCCC TGCTCCTATA ATTGTCAACA 
     1261 CAGACACTTT GGACACTATT CCTTATGTCA ATGGAACAGA AATTGAATAT GAATTTGAAG 
     1321 AAATTACATT GGAGAGGGGA AATTCAGGTC TGGGATTCAG TATTGCTGGA GGGACAGATA 
     1381 ATCCCCACAT TGGAGATGAC CCTGGCATAT TTATTACGAA GATTATTCCA GGAGGTGCTG 
     1441 CGGCAGAGGA TGGCAGACTC AGGGTCAACG ACTGTATCTT GCGGGTGAAT GAAGTTGATG 
     1501 TGTCAGAGGT TTCCCACAGT AAAGCAGTGG AAGCCCTCAA GGAAGCCGGC TCTATTGTGC 
     1561 GGCTGTACGT GCGCAGAAGG CGACCCATAT TGGAGACTGT TGTGGAAATC AAACTTTTTA 
     1621 AAGGTCCAAA AGGTTTAGGC TTCAGTATTG CTGGAGGGGT GGGGAACCAG CACATTCCTG 
     1681 GAGACAACAG CATCTATGTA ACAAAAATTA TAGATGGTGG AGCTGCACAG AAAGATGGAA 
     1741 GGTTGCAAGT AGGAGACAGA CTGCTAATGG TAAATAACTA TAGTTTAGAA GAAGTTACAC 
     1801 ACGAAGAGGC TGTAGCGATA TTGAAGAACA CATCTGATGT TGTTTATCTA AAAGTTGGCA 
     1861 AACCCACCAC TATTTATATG ACTGATCCTT ATGGGCCACC GGATATTACT CACTCTTATT 
     1921 CTCCACCGAT GGAAAATCAT CTACTGTCTG GTAACAATGG CACATTAGAA TACAAGACTT 
     1981 CCCTGCCGCC CATCTCTCCA GGAAGGTACT CACCAATTCC CAAGCACATG CTGGGTGAAG 
     2041 ATGACTACAC CAGGCCGCCG GAACCTGTTT ACAGCACTGT GAATAAACTG TGTGATAAGC 
     2101 CTGCTTCTCC CAGGCACTAT TCCCCTGTTG AGTGTGACAA AAGCTTCCTT CTCTCAACTC 
     2161 CCTACCCCCA CTACCACCTA GGCCTGCTCC CTGACTCTGA CATGACCAGT CATTCCCAGC 
     2221 ACAGTACTGC AACTCGCCAG CCTTCAGTGA CTCTCCAACG GGCCATCTCC CTGGAAGGGG 
     2281 AGCCCCGCAA GGTAGTCCTT CACAAAGGCT CCACTGGCCT GGGCTTCAAC ATTGTGGGCG 
     2341 GGGAAGATGG AGAAGGTATT TTTGTATCCT TCATTCTGGC CGGTGGACCA GCAGACCTGA 
     2401 GTGGGGAGCT CCAGAGAGGA GACCAGATCT TGTCGGTGAA TGGAATCGAT CTTCGAGGAG 
     2461 CATCCCATGA GCAGGCAGCT GCAGCACTGA AGGGGGCTGG ACAGACTGTG ACAATCATAG 
     2521 CACAATATCA ACCTGAAGAT TACGCTCGAT TTGAGGCCAA AATCCATGAC CTACGAGAGC 
     2581 AGATGATGAA CCACAGCATG AGCTCCGGGT CCGGGTCCCT TCGAACCAAT CAGAAACGCT 
     2641 CCCTGTATGT CAGAGCCATG TTTGACTATG ACAAGAGCAA GGACAGTGGG CTGCCCAGCC 
     2701 AAGGACTTAG TTTTAAATAT GGAGACATCC TTCATGTCAT CAATGCCTCT GATGATGAGT 
     2761 GGTGGCAAGC CAGAAGGGTC ACACTAGATG GGGACAGCGA GGAGATGGGC GTCATTCCCA 
     2821 GCAAGCGGAG GGTGGAAAGA AAGGAGCGTG CCCGATTGAA GACAGTGAAG TTCAATGCAA 
     2881 AACCTGGTGT GATTGATTCC AAAGGGGACA TCCCCGGATT AGGTGACGAC GGTTATGGAA 
     2941 CAAAGACTCT GAGAGGACAA GAAGATCTCA TTCTTTCCTA TGAACCTGTC ACGAGGCAGG 
     3001 AAATAAACTA CACGCGGCCA GTGATTATCC TGGGCCCCAT GAAGGATCGA ATCAATGATG 
     3061 ACTTGATATC TGAATTTCCT GATAAATTTG GCTCCTGTGT GCCTCATACT ACGAGGCCAA 
     3121 AGCGTGACTA CGAAGTCGAC GGCAGAGACT ATCACTTTGT CATTTCTAGA GAACAAATGG 
     3181 AGAAAGATAT CCAAGAGCAC AAATTTATAG AAGCTGGCCA GTACAATGAC AATTTATACG 
     3241 GAACCAGTGT GCAATCTGTG AGATTTGTAG CAGAAAGGGG CAAGCACTGT ATACTTGATG 
     3301 TATCAGGAAA TGCTATTAAG CGGTTACAAG TCGCCCAGCT CTATCCCATT GCCATCTTCA 
     3361 TAAAGCCCAA GTCTCTGGAA CCTCTGATGG AGATGAATAA GCGTCTGACA GAGGAACAAG 
     3421 CCAAGAAAAC TTATGACCGG GCGATTAAAC TAGAACAAGA ATTTGGAGAA TATTTTACAG 
     3481 CTATTGTCCA AGGAGATACC TTAGAAGATA TTTACAACCA ATGCAAGCTT GTTATTGAAG 
     3541 AGCAGTCTGG ACCTTTCATC TGGATTCCCT CAAAGGAGAA GTTAACGCGT ACGCGGCCGC 
     3601 TCGAGATGGA GAGCGACGAG AGCGGCCTGC CCGCCATGGA GATCGAGTGC CGCATCACCG 
     3661 GCACCCTGAA CGGCGTGGAG TTCGAGCTGG TGGGCGGCGG AGAGGGCACC CCCGAGCAGG 
     3721 GCCGCATGAC CAACAAGATG AAGAGCACCA AAGGCGCCCT GACCTTCAGC CCCTACCTGC 
     3781 TGAGCCACGT GATGGGCTAC GGCTTCTACC ACTTCGGCAC CTACCCCAGC GGCTACGAGA 
     3841 ACCCCTTCCT GCACGCCATC AACAACGGCG GCTACACCAA CACCCGCATC GAGAAGTACG 
     3901 AGGACGGCGG CGTGCTGCAC GTGAGCTTCA GCTACCGCTA CGAGGCCGGC CGCGTGATCG 
     3961 GCGACTTCAA GGTGATGGGC ACCGGCTTCC CCGAGGACAG CGTGATCTTC ACCGACAAGA 
     4021 TCATCCGCAG CAACGCCACC GTGGAGCACC TGCACCCCAT GGGCGATAAC GATCTGGATG 
     4081 GCAGCTTCAC CCGCACCTTC AGCCTGCGCG ACGGCGGCTA CTACAGCTCC GTGGTGGACA 
     4141 GCCACATGCA CTTCAAGAGC GCCATCCACC CCAGCATCCT GCAGAACGGG GGCCCCATGT 
     4201 TCGCCTTCCG CCGCGTGGAG GAGGATCACA GCAACACCGA GCTGGGCATC GTGGAGTACC 
     4261 AGCACGCCTT CAAGACCCCG GATGCAGATG CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC 
     4321 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4381 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4441 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4501 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4561 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4621 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4681 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4741 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4801 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4861 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4921 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4981 CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5041 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC 
     5101 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA 
     5161 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG 
     5221 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG 
     5281 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG 
     5341 GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 
     5401 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 
     5461 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 
     5521 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 
     5581 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 
     5641 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 
     5701 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 
     5761 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 
     5821 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 
     5881 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC 
     5941 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 
     6001 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CCGACCGCTA TCAGGACATA 
     6061 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 
     6121 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 
     6181 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC 
     6241 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 
     6301 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 
     6361 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 
     6421 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 
     6481 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT 
     6541 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 
     6601 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 
     6661 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 
     6721 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 
     6781 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 
     6841 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 
     6901 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 
     6961 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 
     7021 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 
     7081 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 
     7141 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 
     7201 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 
     7261 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 
     7321 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA 
     7381 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 
     7441 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA 
     7501 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 
     7561 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 
     7621 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 
     7681 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 
     7741 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 
     7801 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 
     7861 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 
     7921 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 
     7981 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 
     8041 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 
     8101 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 
     8161 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 
     8221 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 
     8281 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 
     8341 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 
     8401 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 
     8461 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 
     8521 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 
     8581 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 
     8641 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG 
     8701 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 
     8761 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 
     8821 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 
     8881 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 
     8941 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 
     9001 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA 
     9061 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//