LOCUS       MG222555                9377 bp    DNA     circular  SYN 
DEFINITION  Inpp4a (tGFP-tagged) - Mouse inositol polyphosphate-4-phosphatase type I (Inpp4a) transcript variant 1, (10ug)
ACCESSION   NM_030266
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3858..4562 
                     /label="GFP"  
     misc_feature    4609..5194 
                     /label="PolyA signal"  
     misc_feature    7286..7931 
                     /label="ColE1"  
     misc_feature    5648..6442 
                     /label="Neo"  
     misc_feature    5279..5581 
                     /label="SV40 ori"  
     misc_feature    7946..8806 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3837..3843 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3837 
                     /label="Inpp4a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACAGCAAGA GAGCACAGCC CTCGCCATGG TGCCAGGGCC CGTGCGATGC 
     1081 AGCGGGCTTC CACCATTGAC GTGGCAGCCG ACATGGTTGG CCTCTCTTTG GCAGGAAATA 
     1141 TCCAAGATCC AGATGAGCCC ATTTTAGAAT TCAGCTTAGC TTGCAGTGAG CTGCACACTC 
     1201 CATCGCTAGA TCGAAAACCA AATAGTTTTG TGGCTGTGAG TGTCACCACC CCTCCGCAGG 
     1261 CGTTCTGGAC GAAGCACGCA CAGACGGAGA TCATCGAGGG AACCAACAAC CCTATCTTTC 
     1321 TAAGCAGCAT TGCCTTCTTT CAAGACTCCC TTATCAATCA GATGACCCAG ATCAAGCTGT 
     1381 CTGTGTATGA TGTCAAAGAC AGATCTCAGG GAACAATGTA CTTACTGGGC TCTGGAACAT 
     1441 TCGTGGTCAA AGATCTGCTC CAGGACAGGC ATCACAGATT GCATCTGACA CTGAGGTCTG 
     1501 CAGAGAGTGA CCGTGTCGGC AACATAACTG TGATCGGCTG GCAGATGGAG GAGAAGTCCG 
     1561 ACCAGCAGCC CCCTGTGACC CGGTCTCTGG ACACTGTCAA TGGAAGGATG GTTCTGCCCG 
     1621 TTGATGAAAG CTTGACTGAG GCCTTGGGAA TCCGATCCAA ATATGCTTCT TTGCGAAAAG 
     1681 ACAGCTTACT GAAAGCAGTG TTTGGTGGTG CCATCTGCCG CATGTACCGC TTCCCAACTA 
     1741 CTGATGGTAA CCACCTACGG ATCCTGGAGC AGATGGCAGA GAGCGTCCTC TCCCTGCACG 
     1801 TGCCTCGGCA GTTTGTGAAG CTACTGCTGG AAGAAGATGC AGCCAGGGTA TGTGAGTTGG 
     1861 AAGAGTTGGG GGAGCTGTCC CCTTGCTGGG AGAGCCTCCG GCGCCAGATT GTCACCCAGT 
     1921 ATCAGACTAT CATCCTCACC TACCAGGAGA ATCTGACTGA CCTCCATCAG TACAAAGGTC 
     1981 CTTCCTTTAA AGCAAGCAGT TTGAAAGCAG ATAAAAAGTT AGAATTTGTT CCCACAAACC 
     2041 TGCACATACA ACGGATGCGA GTTCAAGATG ATGGAGGCTC AGATCAGAAC TACGATGTCG 
     2101 TCACTATTGG AGCCCCAGCA GCACACTGCC AAGGTTTTAA GTCAGGAGGT CTTCGAAAAA 
     2161 AGCTGCACAA GTTTGAAGAG GCCAAGAAAC ACAGTTTTGA GGAGTGTTGT ACATCTTCTA 
     2221 GTGGCCAGTC CATCATCTAC ATACCACAGG ACATCACCCG AGCCAAGGAG ATCATTGCCC 
     2281 AGATCAACAC CCTAAAAACC CAGGTCAGCT ACTACGCGGA ACGGCTCTCA AGGGCTGCGA 
     2341 AGGACAGGTC AGCTACTGGC CTTGAGAGGA CTCTCGCCAT CTTGGCAGAC AAGACTCGGC 
     2401 AGTTGGTGAC TGTCTGTGAC TGTAAGCTGC TGGCCAACTC CATCCATGGG CTGAATGCAG 
     2461 CTCGGCCCGA CTACATCGCT TCCAAGGCCT CCCCTACCTC GACTGAGGAG GAGCAGGTGA 
     2521 TGCTTCGGAA TGACCAGGAC ACCCTGATGG CCAGGTGGGC GGGGAGAAGC AGCCGGTCTT 
     2581 CCCTGCAGGT GGACTGGCAT GAGGAGGAGT GGGAGAAAGT GTGGCTGAAT GTGGACAAGA 
     2641 GCCTGGAGTG CATCATTCAG CGGGTGGACA AGCTGCTGCA GAAGGAGCGT CTGCATGGGG 
     2701 AGGGCTGCGA GGATGCTTTC CCCTGTTCGA GCACCTGCTC CAGCAAGAAA GATTGCAGCC 
     2761 CCCCTCCTGA AGAGTCCAGT CCAGGTGAGT GGAGCGAGGC CCTTTACCCT CTGCTGACCA 
     2821 CCCTCACAGA CTGTGTGGCC ATGATGAGCG ACAAGGCCAA GGCAGCCATG GTCTTCCTGC 
     2881 TCATGCAGGA CAGTGCCCCC ACCATTGCCT CGTACCTCAG CCTGCAGTAT CGCCGTGACG 
     2941 TCGTCTTCTG CCAAACCCTG ACAGCCCTCA TCTGTGGCTT TATCATCAAG CTGAGGAACT 
     3001 GCCTGCACGA CGGTGGCTTC CTGCGGCAGC TCTACACCAT CGGCCTTCTG GCCCAGTTTG 
     3061 AGAGCCTGCT GAGCACCTAC GGAGAGGAGT TGGCCATGTT GGAGGACATG AGCCTTGGAA 
     3121 TCATGGACCT GAGGAATGTG ACCTTTAAAG TCACTCAGGC CACTTCGAAT GCATCAAATG 
     3181 ACATGCTGCC GGTCATCACA GGAAACCGGG ATGGCTTTAA CGTGCGGATC CCTCTGCCAG 
     3241 GCCCACTGTT TGACTCCCTC CCCAGAGAGA TCCAGAGTGG CATGCTGCTG CGGGTGCAGC 
     3301 CTGTCCTCTT CAACGTGGGC ATCAATGAGC AACAGACACT GGCTGAGAGG TTTGGAGATA 
     3361 CGTCCTTACA AGAAGTTATC AATGTGGAGA GCTTGGTGCG GTTAAATTCC TACTTTGAGC 
     3421 AGTTTAAGGA GGTTTTGCCG GAGGACTGTC TACCTCGATC TCGGAGTCAG ACCTGCCTTC 
     3481 CAGAGCTGCT GCGGTTCCTG GGACAGAATG TCCATGCACG CAAGAATAAG AATGTGGACA 
     3541 TCCTCTGGCA AGCTGCTGAG GTCTGTCGCC GCCTTAACGG GGTCCGATTC ACCAGCTGCA 
     3601 AGAGTGCCAA GGACCGCACA GCCATGTCGG TGACCCTGGA GCAGTGTCTG ATCCTGCAGC 
     3661 ACGAGCACGG CATGGCCCCG CAGGTCTTCA CACAAGCCCT GGAGTGCATG CGCAGTGAGG 
     3721 GTTGTCGGCG AGAAAACACA ATGAAGAATG TTGGAAGTCG CAAATATGCA TTTAACTCCC 
     3781 TGCAGCTGAA GGCCTTCCCC AAGCATTACA GGCCTCCAGA AGGGACTTAC GGAAAAGTTG 
     3841 AGACAACGCG TACGCGGCCG CTCGAGATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG 
     3901 AGATCGAGTG CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG 
     3961 GAGAGGGCAC CCCCGAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC 
     4021 TGACCTTCAG CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA 
     4081 CCTACCCCAG CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA 
     4141 ACACCCGCAT CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT 
     4201 ACGAGGCCGG CCGCGTGATC GGCGACTTCA AGGTGATGGG CACCGGCTTC CCCGAGGACA 
     4261 GCGTGATCTT CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA 
     4321 TGGGCGATAA CGATCTGGAT GGCAGCTTCA CCCGCACCTT CAGCCTGCGC GACGGCGGCT 
     4381 ACTACAGCTC CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC 
     4441 TGCAGAACGG GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGGATCAC AGCAACACCG 
     4501 AGCTGGGCAT CGTGGAGTAC CAGCACGCCT TCAAGACCCC GGATGCAGAT GCCGGTGAAG 
     4561 AAAGAGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4621 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4681 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4741 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4801 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4861 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4921 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4981 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5041 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5101 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5161 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5221 ATGCCTGTTG AATTGGGTAC GCGGCCAGCT TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT 
     5281 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5341 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5401 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 
     5461 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 
     5521 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 
     5581 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 
     5641 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 
     5701 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 
     5761 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 
     5821 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 
     5881 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 
     5941 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 
     6001 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 
     6061 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 
     6121 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 
     6181 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 
     6241 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 
     6301 GCCGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 
     6361 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 
     6421 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG 
     6481 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA 
     6541 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 
     6601 TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 
     6661 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 
     6721 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA 
     6781 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 
     6841 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 
     6901 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 
     6961 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 
     7021 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 
     7081 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 
     7141 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 
     7201 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 
     7261 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 
     7321 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 
     7381 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 
     7441 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 
     7501 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 
     7561 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 
     7621 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 
     7681 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT 
     7741 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 
     7801 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 
     7861 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 
     7921 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 
     7981 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 
     8041 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 
     8101 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 
     8161 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 
     8221 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 
     8281 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 
     8341 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 
     8401 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 
     8461 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 
     8521 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 
     8581 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 
     8641 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 
     8701 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 
     8761 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 
     8821 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 
     8881 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 
     8941 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 
     9001 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 
     9061 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 
     9121 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 
     9181 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 
     9241 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 
     9301 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 
     9361 AATTTAACGC GAATTTT
//