LOCUS       MG222265                9164 bp    DNA     circular  SYN 
DEFINITION  Vav2 (tGFP-tagged) - Mouse vav 2 oncogene (Vav2), (10ug)
ACCESSION   NM_009500
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3645..4349 
                     /label="GFP"  
     misc_feature    4396..4981 
                     /label="PolyA signal"  
     misc_feature    7073..7718 
                     /label="ColE1"  
     misc_feature    5435..6229 
                     /label="Neo"  
     misc_feature    5066..5368 
                     /label="SV40 ori"  
     misc_feature    7733..8593 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3624..3630 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3624 
                     /label="Vav2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCAGTGG CGGCAATGCG GCCGCTGGCT CATCGACTGC AAGGTCCTGC 
     1081 CGCCCAACCA CCGCGTCGTG TGGCCCTCGG CGGTGGTCTT CGACCTGGCG CAGGCGCTGC 
     1141 GCGACGGCGT CCTTCTGTGC CAGCTGCTGC ACAACCTCTC CCCCGGTTCC ATCGACCTTA 
     1201 AGGACATCAA CTTCCGGCCG CAGATGTCCC AGTTCTTGTG TCTGAAGAAT ATTCGCACCT 
     1261 TCCTCAAAGT CTGCCATGAC AAATTTGGAC TAAGAAACAG TGAGCTGTTT GACCCTTTCG 
     1321 ACCTCTTTGA TGTCCGAGAC TTCGGGAAGG TCATCTCTGC AGTGTCCCGT CTGTCCCTGC 
     1381 ACAGCATCGC ACAGAGCAAA GGGATCAGGC CATTTCCATC AGAGGAGACA GCTGAAAATG 
     1441 ATGACGACGT CTACCGGAGC CTGGAAGAGC TGGCTGACGA GCATGACCTG GGTGAGGACA 
     1501 TCTACGACTG TGTCCCGTGT GAAGATGAAG GAGATGACAT TTATGAGGAC ATCATCAAGG 
     1561 TGGAGGTGCA GCAGCCCATG AAAATGGGCA TGACTGAGGA CGACAAGAGA AGCTGCTGCT 
     1621 TGTTAGAGAT TCAGGAGACC GAGGCCAAGT ACTACCGCAC CCTGGAGGAC ATTGAGAAGA 
     1681 ACTACATGGG TCCCTTGCGG CTGGTGCTGA GCCCGGCGGA TATGGCTGCT GTCTTCATCA 
     1741 ACCTGGAGGA CCTCATCAAG GTGCATCACA GCTTTCTGCG AGCCATCGAT GTGTCCATGA 
     1801 TGGCTGGTGG CAGTACCCTG GCTAAGGTCT TTCTGGAGTT TAAGGAAAGG CTCCTGATCT 
     1861 ATGGAGAGTA CTGTAGCCAC ATGGAACACG CTCAGAGTAC ACTGAACCAG CTCCTCGCCA 
     1921 GCCGAGAGGA CTTCAGGCAG AAAGTGGAGG AGTGCACACT CAGGGTTCAG GATGGCAAGT 
     1981 TCAAGCTGCA AGACCTGCTG GTGGTGCCCA TGCAACGGGT GCTGAAGTAC CACCTGCTGC 
     2041 TCAAGGAGCT CCTGAGCCAT TCTGCAGACC GACCAGAAAG ACAACAGCTC AAAGAAGCCC 
     2101 TGGAAGCCAT GCAGGACTTG GCCATGTACA TCAATGAAGT GAAGCGGGAC AAGGAGACCT 
     2161 TGAAGAAGAT TAGCGAGTTC CAGTGCTCCA TAGAAAACCT GCAAGTGAAG CTGGAGGAAT 
     2221 TTGGGAGGCC AAAGATTGAC GGGGAGCTTA AAGTCCGGTC CATAGTCAAC CACACCAAGC 
     2281 AAGACAGGTA CCTGTTCCTA TTTGACAAGG TGGTCATCGT GTGCAAGAGG AAGGGCTACA 
     2341 GCTATGAGCT GAAGGAGGTC ATTGAGCTGC TCTTCCACAA GATGACCGAT GACCCGATGC 
     2401 ACAACAAGGA CATCAAGAAG TGGTCCTATG GCTTCTACCT GATTCACCTC CAAGGAAAGC 
     2461 AAGGCTTTCA GTTCTTCTGC AAGACGGAAG ACATGAAGCG GAAGTGGATG GAGCAGTTCG 
     2521 AGATGGCCAT GTCAAACATC AAGCCAGATA AGGCCAATGC CAACCATCAT AGCTTCCAGA 
     2581 TGTACACATT CGACAAGACT ACCAACTGCA AAGCCTGCAA GATGTTTCTC AGGGGTACCT 
     2641 TCTACCAGGG ATACCTGTGT ACCAGATGTG GCGTCGGGGC ACACAAGGAA TGCCTGGAGG 
     2701 TGATCCCCCC CTGCAAGATG AGTTCACCTG CAGATGTGGA TGCTCCTGGA GCAGGACCAG 
     2761 GTCCCAAGAT GGTTGCTGTG CAGAATTACC ATGGTAACCC AGCCCCTCCT GGGAAGCCTG 
     2821 TGTTGACCTT CCAGACAGGA GACGTGATAG AACTGCTCCG GGGTGACCCC GATTCTCCTT 
     2881 GGTGGGAAGG GCGGCTGGTT CAAACCAGGA AGTCAGGGTA TTTTCCCAGC TCATCTGTGA 
     2941 AGCCCTGCCC AGTGGATGGA AGGCCGCCTA CTGGCCGCCC ACCATCCCGG GAGATCGATT 
     3001 ACACTGCCTA CCCGTGGTTT GCTGGCAACA TGGAACGGCA GCAGACGGAC AATCTGCTCA 
     3061 AGTCTCATGC CAGTGGGACC TACCTCATCA GAGAGCGTCC AGCAGAGGCA GAACGCTTCG 
     3121 CCATCAGCAT CAAGTTCAAT GATGAGGTGA AACATATCAA GGTGGTGGAG AAAGATAGCT 
     3181 GGATCCACAT CACGGAAGCT AAGAAGTTTG AGAGCCTCCT GGAGCTGGTG GAGTACTACC 
     3241 AGTGTCACTC ACTCAAGGAA AGCTTCAAGC AGTTGGACAC TACACTCAAG TTCCCCTACA 
     3301 AGTCTCGGGA GCGAACCACC AGCAGGGCCT CAAGCCGGTC TCCAGCTTCC TGCGCTTCCT 
     3361 ACAACTTTTC TTTTCTCAGT CCTCAGGGCC TCAGCTTTGC CCCCCAGGCC CCTTCTGCTC 
     3421 CCTTCTGGTC AGTGTTCACA CCGCGGGTCA TCGGCACAGC CGTGGCCAGG TACAACTTTG 
     3481 CTGCTCGAGA CATGCGGGAG CTGTCACTGC GAGAAGGTGA TGTGGTGAAG ATCTACAGCC 
     3541 GAATTGGTGG GGACCAGGGC TGGTGGAAAG GAGAGACAAA TGGGCGGATC GGTTGGTTTC 
     3601 CTTCGACGTA CGTGGAAGAG GAAGGCGTCC AGACGCGTAC GCGGCCGCTC GAGATGGAGA 
     3661 GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG 
     3721 GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA 
     3781 ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA 
     3841 TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC 
     3901 ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG 
     3961 TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG 
     4021 TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA 
     4081 ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC 
     4141 GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT 
     4201 TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC 
     4261 GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA 
     4321 AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4381 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4441 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4501 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4561 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4621 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4681 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4741 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4801 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4861 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4921 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4981 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG 
     5041 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5101 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5161 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 
     5221 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
     5281 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 
     5341 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 
     5401 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 
     5461 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 
     5521 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 
     5581 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 
     5641 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 
     5701 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 
     5761 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 
     5821 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 
     5881 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 
     5941 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 
     6001 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 
     6061 TTCATCGACT GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC 
     6121 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 
     6181 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 
     6241 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 
     6301 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 
     6361 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 
     6421 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 
     6481 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 
     6541 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 
     6601 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 
     6661 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 
     6721 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 
     6781 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
     6841 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     6901 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     6961 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     7021 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     7081 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     7141 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     7201 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     7261 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     7321 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     7381 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     7441 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     7501 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     7561 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     7621 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     7681 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     7741 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     7801 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     7861 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     7921 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     7981 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     8041 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     8101 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     8161 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     8221 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     8281 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     8341 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     8401 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     8461 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     8521 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     8581 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     8641 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     8701 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 
     8761 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 
     8821 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 
     8881 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 
     8941 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 
     9001 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 
     9061 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 
     9121 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//