LOCUS       MG222168                9090 bp    DNA     circular  SYN 
DEFINITION  Nlgn2 (GFP-tagged) - Mouse neuroligin 2 (Nlgn2), (10ug)
ACCESSION   NM_198862
VERSION     NM_198862.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3572..4276 
                     /label="GFP"  
     misc_feature    4323..4908 
                     /label="PolyA signal"  
     misc_feature    7000..7645 
                     /label="ColE1"  
     misc_feature    5362..6156 
                     /label="Neo"  
     misc_feature    4993..5295 
                     /label="SV40 ori"  
     misc_feature    7660..8520 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3528..3534 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3528 
                     /label="Nlgn2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCATG TGGCTCCTGG CGTTGTGTCT GGTGGGGCTG GCTGGGGCTC AACGGGGAGG 
     1081 AGGGGGTCCC GGCGGCGGCG CCCCGGGCGG CCCAGGCCTG GGCCTCGGCA GCCTCGGGGA 
     1141 GGAGCGCTTC CCCGTGGTGA ACACAGCCTA CGGGCGAGTG CGCGGTGTGC GGCGCGAGCT 
     1201 CAACAACGAG ATCCTGGGCC CGGTCGTGCA GTTCTTGGGC GTGCCCTACG CCACGCCGCC 
     1261 CTTGGGCGCC CGCCGCTTCC AGCCGCCTGA GGCACCTGCC TCGTGGCCCG GCGTGCGCAA 
     1321 CGCCACCACC CTGCCGCCCG CCTGCCCGCA GAACCTGCAC GGGGCCCTGC CGGCCATCAT 
     1381 GCTGCCTGTG TGGTTCACCG ACAACTTGGA GGCGGCCGCC ACCTACGTGC AGAACCAGAG 
     1441 CGAGGACTGC CTGTACCTCA ACCTCTACGT GCCCACTGAG GACGGTCCGC TCACAAAAAA 
     1501 ACGTGACGAG GCGACGCTCA ATCCGCCAGA CACAGATATC CGGGACTCTG GGAAGAAACC 
     1561 GGTCATGCTG TTTCTACACG GCGGCTCCTA CATGGAAGGC ACCGGGAACA TGTTTGACGG 
     1621 CTCAGTCCTG GCTGCCTATG GCAATGTCAT CGTAGTCACA CTCAACTACC GTCTTGGGGT 
     1681 GCTCGGTTTT CTCAGCACTG GTGACCAGGC TGCAAAAGGC AACTATGGGC TCCTGGACCA 
     1741 GATCCAGGCC CTGCGCTGGC TCAGTGAAAA CATTGCCCAC TTTGGAGGTG ACCCTGAACG 
     1801 CATCACTATC TTTGGGTCTG GTGCAGGGGC CTCCTGTGTC AACTTGCTGA TCCTTTCCCA 
     1861 CCACTCAGAA GGACTGTTCC AGAAGGCCAT TGCTCAAAGT GGTACTGCCA TTTCCAGCTG 
     1921 GTCTGTCAAC TACCAGCCGC TCAAGTACAC GCGGCTGCTG GCGGCCAAAG TGGGCTGTGA 
     1981 CCGAGAAGAC AGCACTGAAG CTGTGGAGTG TCTGCGCCGG AAGTCTTCCC GGGAGCTAGT 
     2041 GGACCAGGAT GTACAGCCTG CCCGCTACCA CATTGCCTTT GGGCCTGTGG TGGACGGCGA 
     2101 CGTAGTCCCT GATGACCCCG AGATCCTCAT GCAACAGGGG GAATTCCTCA ACTACGACAT 
     2161 GCTCATTGGT GTCAACCAGG GAGAGGGTCT CAAGTTCGTG GAGGACTCTG CAGAGAGTGA 
     2221 GGACGGTGTG TCTGCCAGCG CCTTTGACTT CACCGTCTCC AACTTTGTGG ACAACTTGTA 
     2281 CGGGTACCCA GAAGGCAAGG ACGTGCTTCG AGAGACCATC AAGTTCATGT ACACGGACTG 
     2341 GGCTGACAGG GACAATGGCG AGATGCGGCG GAAGACCCTG CTGGCGCTCT TTACCGACCA 
     2401 CCAGTGGGTC GCCCCGGCTG TGGCCACCGC CAAGCTGCAT GCCGACTACC AGTCCCCCGT 
     2461 CTACTTTTAC ACTTTCTACC ACCACTGCCA GGCAGAGGGC CGGCCAGAGT GGGCAGACGC 
     2521 AGCGCACGGG GACGAGCTGC CCTACGTCTT TGGTGTCCCC ATGGTGGGCG CCACTGACCT 
     2581 CTTCCCCTGC AACTTCTCCA AGAACGATGT CATGCTCAGC GCAGTAGTCA TGACCTATTG 
     2641 GACCAACTTC GCCAAGACTG GTGACCCCAA CCAGCCTGTG CCACAGGACA CCAAGTTCAT 
     2701 CCACACCAAG CCCAACCGCT TTGAAGAGGT AGTGTGGAGC AAGTTCAACA GCAAGGAAAA 
     2761 GCAGTATCTG CACATAGGCT TGAAACCACG CGTGCGCGAC AACTACCGTG CCAACAAGGT 
     2821 GGCCTTCTGG CTGGAGCTCG TGCCCCACCT GCACAACCTG CACACAGAGC TCTTCACCAC 
     2881 CACCACTCGC CTGCCTCCCT ATGCCACACG CTGGCCACCT CGCACACCTG GTCCTGGCAC 
     2941 TTCCGGCACA CGCCGTCCTC CCCCACCTGC CACTCTGCCA CCTGAGTCTG ATATTGACCT 
     3001 AGGCCCAAGG GCCTATGACC GCTTCCCCGG TGACTCGAGG GACTACTCCA CGGAGCTAAG 
     3061 CGTGACTGTG GCAGTGGGTG CCTCCCTCCT CTTCCTCAAC ATCCTTGCCT TTGCCGCCCT 
     3121 CTATTACAAG CGGGACCGGC GCCAGGAGCT GCGGTGCCGG AGGCTTAGCC CACCAGGAGG 
     3181 CTCAGGCTCA GGTGTGCCTG GTGGGGGCCC CCTGCTTCCC ACTGCTGGCC GTGAGCTACC 
     3241 CCCGGAGGAG GAGCTAGTAT CGCTGCAGCT GAAGCGGGGT GGTGGTGTTG GGGCGGACCC 
     3301 TGCTGAGGCC CTGCGCCCTG CCTGTCCACC CGACTATACC CTGGCCTTGC GCCGGGCACC 
     3361 GGACGATGTG CCTCTCTTGG CCCCCGGGGC CCTAACCCTG CTGCCTAGTG GCCTGGGGCC 
     3421 CCCGCCCCCG CCCCCACCCC CTTCTCTCCA TCCCTTTGGG CCCTTCCCAC CCCCACCCCC 
     3481 TACTGCTACC AGCCACAACA ACACGCTACC CCATCCCCAC TCCACCACTC GGGTAAAGCT 
     3541 TAACTAGTTA GCGGACCGAC GCGTACGCGG CCGCTCGAGA TGGAGAGCGA CGAGAGCGGC 
     3601 CTGCCCGCCA TGGAGATCGA GTGCCGCATC ACCGGCACCC TGAACGGCGT GGAGTTCGAG 
     3661 CTGGTGGGCG GCGGAGAGGG CACCCCCGAG CAGGGCCGCA TGACCAACAA GATGAAGAGC 
     3721 ACCAAAGGCG CCCTGACCTT CAGCCCCTAC CTGCTGAGCC ACGTGATGGG CTACGGCTTC 
     3781 TACCACTTCG GCACCTACCC CAGCGGCTAC GAGAACCCCT TCCTGCACGC CATCAACAAC 
     3841 GGCGGCTACA CCAACACCCG CATCGAGAAG TACGAGGACG GCGGCGTGCT GCACGTGAGC 
     3901 TTCAGCTACC GCTACGAGGC CGGCCGCGTG ATCGGCGACT TCAAGGTGAT GGGCACCGGC 
     3961 TTCCCCGAGG ACAGCGTGAT CTTCACCGAC AAGATCATCC GCAGCAACGC CACCGTGGAG 
     4021 CACCTGCACC CCATGGGCGA TAACGATCTG GATGGCAGCT TCACCCGCAC CTTCAGCCTG 
     4081 CGCGACGGCG GCTACTACAG CTCCGTGGTG GACAGCCACA TGCACTTCAA GAGCGCCATC 
     4141 CACCCCAGCA TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT TCCGCCGCGT GGAGGAGGAT 
     4201 CACAGCAACA CCGAGCTGGG CATCGTGGAG TACCAGCACG CCTTCAAGAC CCCGGATGCA 
     4261 GATGCCGGTG AAGAAAGAGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA 
     4321 TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC 
     4381 TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT 
     4441 GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA 
     4501 GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC 
     4561 TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA 
     4621 GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG 
     4681 ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC 
     4741 ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT 
     4801 TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG 
     4861 CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG 
     4921 GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA GCTTGGCTGT GGAATGTGTG 
     4981 TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA 
     5041 TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT 
     5101 GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC 
     5161 GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT 
     5221 TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT 
     5281 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC 
     5341 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG 
     5401 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG 
     5461 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA 
     5521 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT 
     5581 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA 
     5641 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC 
     5701 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC 
     5761 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC 
     5821 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT 
     5881 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA 
     5941 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG 
     6001 CCGGCTGGGT GTGGCCGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA 
     6061 AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA 
     6121 TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG 
     6181 TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC 
     6241 GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC 
     6301 CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT 
     6361 AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG 
     6421 CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG 
     6481 ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT 
     6541 CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC 
     6601 TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA 
     6661 AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT 
     6721 ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG 
     6781 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC 
     6841 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG 
     6901 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA 
     6961 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC 
     7021 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC 
     7081 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG 
     7141 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC 
     7201 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA 
     7261 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG 
     7321 AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG 
     7381 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT 
     7441 GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA 
     7501 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 
     7561 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA 
     7621 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA 
     7681 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC 
     7741 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG 
     7801 ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA 
     7861 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT 
     7921 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT 
     7981 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC 
     8041 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC 
     8101 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA 
     8161 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG 
     8221 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG 
     8281 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA 
     8341 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA 
     8401 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA 
     8461 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA 
     8521 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8581 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8641 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8701 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8761 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8821 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8881 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8941 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9001 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9061 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//