LOCUS       MG221659                9290 bp    DNA     circular  SYN 
DEFINITION  Hcn1 (tGFP-tagged) - Mouse hyperpolarization-activated cyclic nucleotide-gated K+ 1 (Hcn1), (10ug)
ACCESSION   NM_010408
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3771..4475 
                     /label="GFP"  
     misc_feature    4522..5107 
                     /label="PolyA signal"  
     misc_feature    7199..7844 
                     /label="ColE1"  
     misc_feature    5561..6355 
                     /label="Neo"  
     misc_feature    5192..5494 
                     /label="SV40 ori"  
     misc_feature    7859..8719 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3750..3756 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3750 
                     /label="Hcn1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAGGCGGC GGCAAACCCA ACTCCGCGTC CAACAGCCGC GACGATGGCA 
     1081 ACAGCGTCTT CCCCTCCAAG GCGCCCGCGA CGGGGCCGGT GGCGGCCGAC AAGCGCCTGG 
     1141 GGACCCCGCC GGGGGGCGGC GCGGCCGGGA AGGAACATGG CAACTCCGTG TGCTTCAAGG 
     1201 TGGACGGCGG CGGAGGAGAG GAGCCGGCGG GCAGCTTCGA GGATGCCGAG GGGCCCCGGC 
     1261 GGCAGTATGG TTTCATGCAG AGGCAGTTCA CCTCCATGCT GCAGCCTGGG GTCAACAAAT 
     1321 TCTCCCTCCG CATGTTTGGG AGCCAGAAGG CGGTGGAGAA GGAGCAGGAA AGGGTTAAAA 
     1381 CTGCAGGCTT CTGGATTATC CATCCGTACA GTGACTTCAG GTTTTATTGG GATTTAATCA 
     1441 TGCTTATAAT GATGGTTGGA AATTTGGTCA TCATACCAGT TGGAATCACG TTCTTCACAG 
     1501 AGCAGACGAC AACACCGTGG ATTATTTTCA ACGTGGCATC CGATACTGTT TTCCTGTTGG 
     1561 ACTTAATCAT GAATTTTAGG ACTGGGACTG TCAATGAAGA CAGCTCGGAA ATCATCCTGG 
     1621 ACCCTAAAGT GATCAAGATG AATTATTTAA AAAGCTGGTT TGTGGTGGAC TTCATCTCAT 
     1681 CGATCCCGGT GGATTATATC TTTCTCATTG TAGAGAAAGG GATGGACTCA GAAGTTTACA 
     1741 AGACAGCCAG AGCACTTCGT ATCGTGAGGT TTACAAAAAT TCTCAGTCTC TTGCGGTTAT 
     1801 TACGCCTTTC AAGGTTAATC AGATACATAC ACCAGTGGGA AGAGATATTC CACATGACCT 
     1861 ATGACCTCGC CAGTGCTGTG GTGAGGATCT TCAACCTCAT TGGCATGATG CTGCTTCTGT 
     1921 GCCACTGGGA TGGCTGTCTT CAGTTCCTGG TTCCCCTGCT GCAGGACTTC CCACCAGATT 
     1981 GCTGGGTTTC TCTGAATGAA ATGGTTAATG ATTCCTGGGG AAAACAATAT TCCTACGCAC 
     2041 TCTTCAAAGC TATGAGTCAC ATGCTGTGCA TTGGTTATGG CGCCCAAGCC CCTGTCAGCA 
     2101 TGTCTGACCT CTGGATTACC ATGCTGAGCA TGATTGTGGG CGCCACCTGC TACGCAATGT 
     2161 TTGTTGGCCA TGCCACAGCT TTGATCCAGT CTTTGGACTC TTCAAGGAGG CAGTATCAAG 
     2221 AGAAGTATAA GCAAGTAGAG CAATACATGT CATTCCACAA GTTACCAGCT GACATGCGCC 
     2281 AGAAGATACA TGATTACTAT GAGCACCGAT ACCAAGGCAA GATCTTCGAT GAAGAAAATA 
     2341 TTCTCAGTGA GCTTAATGAT CCTCTGAGAG AGGAAATAGT CAACTTCAAC TGCCGGAAAC 
     2401 TGGTGGCAAC TATGCCTCTT TTTGCTAACG CCGATCCCAA TTTCGTGACG GCCATGCTGA 
     2461 GCAAGCTGAG ATTTGAGGTG TTCCAGCCCG GAGACTATAT CATTCGAGAA GGAGCTGTGG 
     2521 GGAAGAAAAT GTATTTCATC CAGCACGGTG TTGCTGGCGT TATCACCAAG TCCAGTAAAG 
     2581 AAATGAAGCT GACAGATGGC TCTTACTTCG GAGAGATATG CCTGCTGACC AAGGGCCGGC 
     2641 GCACTGCCAG TGTCCGAGCT GATACCTACT GTCGTCTTTA CTCCCTTTCG GTGGACAATT 
     2701 TCAATGAGGT CTTGGAGGAA TATCCAATGA TGAGAAGAGC CTTTGAGACA GTTGCTATTG 
     2761 ACCGACTCGA TCGGATAGGC AAGAAAAACT CTATTCTCCT GCAGAAGTTC CAGAAGGATC 
     2821 TAAACACTGG TGTTTTCAAC AACCAGGAGA ACGAGATCCT GAAGCAGATC GTGAAGCATG 
     2881 ACCGAGAGAT GGTACAAGCT ATCCCTCCAA TCAACTATCC TCAAATGACA GCCCTCAACT 
     2941 GCACATCTTC AACCACCACC CCAACCTCCC GCATGAGGAC CCAATCTCCG CCAGTCTACA 
     3001 CCGCAACCAG CCTGTCTCAC AGCAATCTGC ACTCACCCAG TCCCAGCACA CAGACGCCCC 
     3061 AACCCTCAGC CATCCTTTCA CCCTGCTCCT ATACCACAGC AGTCTGCAGT CCTCCTATAC 
     3121 AGAGCCCCCT GGCCACACGA ACTTTCCATT ATGCCTCTCC CACTGCGTCC CAGCTGTCAC 
     3181 TCATGCAGCA GCCTCAGCAG CAACTACCGC AGTCCCAGGT ACAGCAGACT CAGACTCAGA 
     3241 CTCAGCAGCA GCAGCAGCAA CAGCAGCAGC AGCAGCAGCA GCAACAGCAA CAACAGCAGC 
     3301 AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCACAGA 
     3361 CACCTGGTAG CTCCACACCG AAAAATGAAG TGCACAAGAG CACACAAGCC CTTCATAACA 
     3421 CCAACCTGAC CAAAGAAGTC AGGCCCCTTT CCGCCTCGCA GCCTTCTCTG CCCCATGAGG 
     3481 TCTCCACTTT GATCTCCAGA CCTCATCCCA CTGTGGGCGA ATCCCTGGCC TCTATCCCTC 
     3541 AACCCGTGGC AGCAGTCCAC AGCACTGGCC TTCAGGCAGG GAGCAGGAGC ACAGTGCCAC 
     3601 AACGTGTCAC CTTGTTCCGA CAGATGTCCT CGGGAGCCAT CCCCCCCAAC CGAGGAGTGC 
     3661 CTCCAGCACC CCCTCCACCA GCAGCTGTGC AGAGAGAGTC TCCCTCAGTC CTAAATACAG 
     3721 ACCCAGATGC AGAAAAACCC CGTTTTGCTT CGAATTTAAC GCGTACGCGG CCGCTCGAGA 
     3781 TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA TGGAGATCGA GTGCCGCATC ACCGGCACCC 
     3841 TGAACGGCGT GGAGTTCGAG CTGGTGGGCG GCGGAGAGGG CACCCCCGAG CAGGGCCGCA 
     3901 TGACCAACAA GATGAAGAGC ACCAAAGGCG CCCTGACCTT CAGCCCCTAC CTGCTGAGCC 
     3961 ACGTGATGGG CTACGGCTTC TACCACTTCG GCACCTACCC CAGCGGCTAC GAGAACCCCT 
     4021 TCCTGCACGC CATCAACAAC GGCGGCTACA CCAACACCCG CATCGAGAAG TACGAGGACG 
     4081 GCGGCGTGCT GCACGTGAGC TTCAGCTACC GCTACGAGGC CGGCCGCGTG ATCGGCGACT 
     4141 TCAAGGTGAT GGGCACCGGC TTCCCCGAGG ACAGCGTGAT CTTCACCGAC AAGATCATCC 
     4201 GCAGCAACGC CACCGTGGAG CACCTGCACC CCATGGGCGA TAACGATCTG GATGGCAGCT 
     4261 TCACCCGCAC CTTCAGCCTG CGCGACGGCG GCTACTACAG CTCCGTGGTG GACAGCCACA 
     4321 TGCACTTCAA GAGCGCCATC CACCCCAGCA TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT 
     4381 TCCGCCGCGT GGAGGAGGAT CACAGCAACA CCGAGCTGGG CATCGTGGAG TACCAGCACG 
     4441 CCTTCAAGAC CCCGGATGCA GATGCCGGTG AAGAAAGAGT TTAAACGGCC GGCCGCGGTC 
     4501 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4561 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4621 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4681 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4741 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4801 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4861 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4921 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4981 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5041 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5101 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5161 GCTTGGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     5221 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC 
     5281 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG 
     5341 CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT 
     5401 GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC 
     5461 CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT 
     5521 TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA 
     5581 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC 
     5641 TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG 
     5701 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG 
     5761 GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT 
     5821 GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG 
     5881 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG 
     5941 CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA 
     6001 GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG 
     6061 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT 
     6121 CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT 
     6181 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCCGACC GCTATCAGGA CATAGCGTTG 
     6241 GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT 
     6301 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC 
     6361 TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC 
     6421 GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG 
     6481 ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA 
     6541 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA 
     6601 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT 
     6661 ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT 
     6721 TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA 
     6781 AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC 
     6841 TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG 
     6901 CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC 
     6961 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 
     7021 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 
     7081 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 
     7141 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 
     7201 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 
     7261 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 
     7321 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 
     7381 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 
     7441 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG 
     7501 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT 
     7561 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 
     7621 CCGGCAAACA AACCACCGCT GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 
     7681 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 
     7741 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 
     7801 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 
     7861 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 
     7921 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 
     7981 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG 
     8041 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 
     8101 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 
     8161 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 
     8221 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 
     8281 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 
     8341 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 
     8401 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 
     8461 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 
     8521 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 
     8581 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 
     8641 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 
     8701 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 
     8761 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 
     8821 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT 
     8881 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG 
     8941 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC 
     9001 AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC 
     9061 CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT 
     9121 TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA 
     9181 CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG 
     9241 CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//