LOCUS       MG221191                9578 bp    DNA     circular  SYN 
DEFINITION  Pcdhac2 (tGFP-tagged) - Mouse protocadherin alpha subfamily C 2 (Pcdhac2), (10ug)
ACCESSION   NM_001003672
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4059..4763 
                     /label="GFP"  
     misc_feature    4810..5395 
                     /label="PolyA signal"  
     misc_feature    7487..8132 
                     /label="ColE1"  
     misc_feature    5849..6643 
                     /label="Neo"  
     misc_feature    5480..5782 
                     /label="SV40 ori"  
     misc_feature    8147..9007 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4038..4044 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4038 
                     /label="Pcdhac2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCAGGCG GGAGCCAGAC CTGGGGCGAC AGAGCATCCG CGACCCCTGC 
     1081 GGCCCTTGCC CTGGCTGCTG CTTCTGTCTT TCCGACTGCT GCTGGTGCTG CTGCCAGGCC 
     1141 CAGCGGCCTC CCAGCTACGA TACTCCGTGC CGGAGGAGCA GAGCCCCGGC GCGCTCGTGG 
     1201 GCAACGTGGC CAGAGCCTTG GGGCTAGAAC TGCGGCGCTT GGGACCTGGC TGTCTGCGCA 
     1261 TCAACCATTT GGGTGCACCC AGCCCGCGCT ACCTGGAACT GGACTTGACC AATGGAGCGC 
     1321 TCTTCGTCAA CGAGCGGATA GATCGCGAGG CGCTGTGCGA GCAGCGGCCT CGCTGCCTGC 
     1381 TCAGCTTGGA AGTGCTGGCT CACAACCCTG TGGCAGTGAG CGCGATCGAG GTGGAAATAC 
     1441 TGGACATCAA TGACAACTCT CCGCGCTTCC CGCGGCCGGA TTATCAGCTT CAGGTAAGCG 
     1501 AATCCGTGGC ACCTGGAGCG CGGTTTCACA TAGAGAGCGC ACAGGACCCT GACGTCGGTG 
     1561 CCAACTCAGT GCAGACCTAC GAGCTCAGCC CCAGCGAGCA CTTTGAGCTG GATCTTAAAC 
     1621 CCTTGCAGGA GAATAGCAAG GTGCTGGAGC TCGTGCTGCG GAAGGGCCTA GACCGGGAGC 
     1681 AGACTGCCTT GCACTACTTG GTTCTCACTG CGGTGGATGG GGGCATCCCA GCTCGCTCAG 
     1741 GCACAGCTCA GATCGCCGTT CGTGTCCTGG ACACGAATGA CAATTCTCCT GCCTTTGACC 
     1801 AATCCACTTA CCGCGTCCAG CTGCGAGAGG ATGCTCCTCC GGGCACATTG GTAGTGAAGT 
     1861 TGAATGCCTC GGACCCGGAT GAGGGTTCTA ACGGTGAACT CAGGTACTCC TTGAGCAGCT 
     1921 ACACATCAGA CCGGGAGAGA CAGCTCTTCA GCATCGATGT GACTACAGGG GAAGTGAGAG 
     1981 TAAGTGGGAC TCTGGATTAT GAGGAGTCTT CCTCCTACCA GATCTATGTG CAAGCTACTG 
     2041 ACCGGGGACC GGTACCCATG GCGGGACACT GCAAGGTGTT GGTGGACATT ATAGATGTGA 
     2101 ATGACAACGC TCCAGAGGTG GTGCTCACAG ACTTATACAG CCCAGTGCCT GAGGATGTTG 
     2161 CCCTCAACAC TGTTGTGGCC CTTCTCAGTG TGAATGACCA AGATTCAGGC TCCAACAGGA 
     2221 AAGTAAGCCT GGGTCTGGAG GCTTCACTGC CCTTCCGGCT GAATGGCTTT GGAAACTCCT 
     2281 ATACCCTGGT GGTGAGTGGC CCATTGGATC GGGAGCGGGT AGCTGCCTAC AACATCACAG 
     2341 TGACAGCTAC TGATGGGGGA GTACCACCAC TCACATCTCA AAGGACATTG CAGGTTGAGA 
     2401 TCTCTGACAT AAATGACAAT CCACCAAGCT TCCTGAAGGA CTCCTATTCC ATCTACATCG 
     2461 AGGAAAACAA TTTACCAGGT GTGTTGCTCT GCACTGTGCA AGCCACAGAC CCAGATCAAA 
     2521 AGGAGAATGC AGAGGTGACC TACTCACTCC TAGACAGGGA GATTCAAGGG CTGCCAGTCA 
     2581 CCTCCTATGT CTCCATTAAC AGTGCCAGCG GCAGCCTTTA TGCTGTCAAC TCCTTTGACT 
     2641 ATGAGAAATT TCGAGAGTTT TTTGTGACTG TGGAGGCCCA GGATAAGGGG AGGCCACCAC 
     2701 TAAGCAGCAC TGTGACCGCC AACGTGTATG TGGTGGACGT GAATGACCAC GCCCCCCATA 
     2761 TTCTATACCC TACCTCAACT AATACATCAG CAGCCATTGA AATGGTGCCA CGGACTGCCC 
     2821 CTGCTGGCTA CCTGGTCACC AAAGTCATAG CCATGGATTC AGACTCTGGG CAGAATGCTT 
     2881 GGCTCTTTTA CCATTTAGTC CCAACGTCTG ACTCGGACCT TTTTAAAGTA GAACTTCACA 
     2941 CAGGAGAAAT TAGGATTACC AGGAAGATAG GAGATGAGAG TGGCACCACT TTCAACCTGA 
     3001 CCGTGGTGGT CCGTGACAAT GGAGAGCCAC CTCTATCAGC TACCGTGGCC ATCACAGTAG 
     3061 CTGTGGTGGA CAGGGTTTCT AAAATGCTCC CTGACACTCA GAGACATATA AAGAGTCCTC 
     3121 GGACATACTC TGAGATCACA CTTTATCTGA TAATAGCATT AAGCACAGTG TCTTTTATAT 
     3181 TCCTTTTGAC AATCATTGTT CTGAGCATCA TTAAGTGCTA CCGCTACACT GCTTATGGCA 
     3241 CGGCATGCTG TGGGGGCTTC TGTGGAGTGA GGGAGCGCTG CCCTGCAGAG CTGTACAAGC 
     3301 AGGCCAATAA CAATATCGAT GCCAGGATAC CACATGGCCT CAAAGTACAG CCTCACTTCA 
     3361 TTGAGGTTCG AGGCAATGGC TCCCTCACCA AGACCTACTG CTACAAGGCC TGCCTGACAG 
     3421 CAGGCTCAGG GAGTGATACT TTCATGTTTT ACAACACAGG GGCCCAGACA GGGCCAGGGC 
     3481 CTGGGGGAGC CCAAGCCTCA GTGACTGACA ACAGGCAACT CACCGGCCAA AGTAGGCACA 
     3541 GTACCGGGAA CCTGATTATC CTAAAAAATG ATGCTGGCTC TCAAAATGAG CCCCGGCAGC 
     3601 CCAACCCTGA CTGGCGCTAC TCTGCCTCGC TAAGAGCAGG CATGCACAGC TCTGTGCACC 
     3661 TGGAGGAGGC TGGCATTCTA CGGGCTGGTC CAGGAGGGCC TGATCAGCAG TGGCCAACAG 
     3721 TATCCAGTGC AACACCAGAA CCTGAGGCAG GAGAGGTGTC CCCTCCGGTG GGCGCCGGTG 
     3781 TCAACAGCAA CAGCTGGACC TTTAAATACG GACCAGGCAA CCCCAAACAG TCCGGTCCCG 
     3841 GTGAGTTGCC AGACAAATTC ATTATCCCAG GATCTCCTGC AATCATCTCC ATCCGGCAGG 
     3901 AGCCTGCTAA CAACCAAATT GACAAAAGCG ATTTTATAAC CTTCGGCAAA AAGGAGGAGA 
     3961 CCAAGAAAAA GAAGAAAAAG AAGAAGGGTA ACAAGACCCA GGAGAAAAAA GAGAAAGGGA 
     4021 ACAGCACGAC GGACAACAGT GACCAGACGC GTACGCGGCC GCTCGAGATG GAGAGCGACG 
     4081 AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG 
     4141 AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCGAGCA GGGCCGCATG ACCAACAAGA 
     4201 TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT 
     4261 ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA 
     4321 TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC 
     4381 ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGATGG 
     4441 GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA 
     4501 CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGATCTGGA TGGCAGCTTC ACCCGCACCT 
     4561 TCAGCCTGCG CGACGGCGGC TACTACAGCT CCGTGGTGGA CAGCCACATG CACTTCAAGA 
     4621 GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG 
     4681 AGGAGGATCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC 
     4741 CGGATGCAGA TGCCGGTGAA GAAAGAGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4801 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4861 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4921 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4981 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5041 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5101 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5161 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5221 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5281 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5341 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5401 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC TTGGCTGTGG 
     5461 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5521 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     5581 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 
     5641 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 
     5701 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 
     5761 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 
     5821 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 
     5881 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 
     5941 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 
     6001 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 
     6061 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 
     6121 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 
     6181 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 
     6241 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 
     6301 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 
     6361 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 
     6421 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 
     6481 GACTGTGGCC GGCTGGGTGT GGCCGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 
     6541 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 
     6601 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 
     6661 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 
     6721 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 
     6781 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 
     6841 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 
     6901 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 
     6961 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 
     7021 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 
     7081 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 
     7141 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 
     7201 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 
     7261 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 
     7321 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 
     7381 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 
     7441 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 
     7501 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 
     7561 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 
     7621 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 
     7681 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 
     7741 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 
     7801 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 
     7861 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 
     7921 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 
     7981 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 
     8041 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 
     8101 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 
     8161 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 
     8221 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 
     8281 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 
     8341 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 
     8401 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 
     8461 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 
     8521 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 
     8581 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 
     8641 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 
     8701 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 
     8761 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 
     8821 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 
     8881 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 
     8941 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 
     9001 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 
     9061 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 
     9121 GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 
     9181 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 
     9241 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 
     9301 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 
     9361 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 
     9421 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 
     9481 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 
     9541 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//