LOCUS       MG220414                9542 bp    DNA     circular  SYN 
DEFINITION  Clcn1 (tGFP-tagged) - Mouse chloride channel 1 (Clcn1), (10ug)
ACCESSION   NM_013491
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4023..4727 
                     /label="GFP"  
     misc_feature    4774..5359 
                     /label="PolyA signal"  
     misc_feature    7451..8096 
                     /label="ColE1"  
     misc_feature    5813..6607 
                     /label="Neo"  
     misc_feature    5444..5746 
                     /label="SV40 ori"  
     misc_feature    8111..8971 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4002..4008 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4002 
                     /label="Clcn1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCGGTCC CAGTCCCAGC GGCATGGAGG GGAACAGAGC TGGTGGGGCA 
     1081 GTGCCCCCCA GTACCAGTAC ATGCCCTTTG AACATTGTAC CAGCTACGGA CTGCCCTCAG 
     1141 AGAATGGGGG CCTTCAGCAC CGGCCCCGAA AGGACATGGG TCCCAGGCAC AATGCCCACC 
     1201 CAACACAGAT ATATGGCCAT CAAAAAGAAC AATATTCATA TAAGGCACAG GACGGGGGAA 
     1261 TGCCCAAGAA GATGGGCTCC AGTTCTACCA TGGACAGCTT GGATGAGGAC CACTATTCTA 
     1321 AATGTCAAGA CTGTGTCCAT CGCCTGGGAC GTGTGCTGAG AAGGAAGCTG GGGGAAGATT 
     1381 GGATCTTTCT TGTGCTCCTG GGCCTCCTGA TGGCTCTAGT CAGCTGGTGC ATGGACTATG 
     1441 TTAGTGCCAA GAGCCTTCAG GCCTACAAGT GGACCTATGC CCAGATGAAG CCTAGCCTTC 
     1501 CTCTGCAGTA CCTGGCCTGG GTCACCTTCC CACTTATCCT GATCCTCTTC AGCGCCCTCT 
     1561 TTTGCCAACT CATCTCTCCC CAGGCTGTGG GCTCTGGAAT CCCTGAGATG AAAACAATTC 
     1621 TTCGTGGTGT TGTCTTGAAG GAATACCTCA CACTCAAGGC CTTTGTAGCC AAGGTGGTAG 
     1681 CTTTGACAGC TGGACTGGGC AGTGGCATCC CTGTGGGGAA AGAGGGTCCC TTTGTTCACA 
     1741 TCGCCAGCAT CTGTGCTGCT GTCCTCAGCA AGTTTATGTC CATGTTCTCT GGTGTCTATG 
     1801 AGCAGCCATA CTATTACACT GACATCCTGA CAGTGGGCTG TGCCGTGGGG GTCGGTTGCT 
     1861 GTTTTGGAAC ACCACTTGGA GGAGTGCTAT TTAGCATCGA GGTCACCTCT ACCTACTTTG 
     1921 CTGTTCGGAA TTACTGGCGA GGATTCTTTG CAGCCACATT CAGTGCCTTT GTGTTCCGTG 
     1981 TCCTGGCCGT TTGGAACAAG GATGCTGTCA CCATCACTGC TCTGTTCAGA ACGAATTTCC 
     2041 GAATGGATTT CCCCTTTGAC CTGAAGGAAC TCCCAGCTTT TGCTGTCATT GGGATTTGCT 
     2101 GCGGGTTCTT GGGAGCTGTT TTCGTTTATC TGCATCGCCA AGTCATGCTC GGTGTCCGAA 
     2161 AGCACAAGTG TCTCAGCCAA TTTCTTGCTA AGCACCGCCT GCTATATCCT GGAATTGTTA 
     2221 CCTTTGTCAT CGCCTCGCTC ACATTTCCAC CAGGAATGGG TCAATTCATG GCTGGAGAGC 
     2281 TGATGCCCCG TGAAGCTATC AGTACCCTCT TTGACAACAA CACATGGGTA AAGCACATAG 
     2341 GTGACCCCCA AAGCTTGGGC CAGTCAGCTG TGTGGCTTCA CCCCCAGGTC AACGTGATCA 
     2401 TCATCATCCT TCTCTTCTTC GTTATGAAGT TTTGGATGTC CATTGTAGCC ACCACTATGC 
     2461 CCATACCCTG TGGAGGCTTC ATGCCTGTTT TTGTGCTAGG AGCTGCATTT GGAAGGCTGG 
     2521 TAGGAGAGAT CATGGCCATG CTATTCCCTG AGGGTATCTT ATTTGATGAT ATCATCTATA 
     2581 AGATCTTACC TGGGGGCTAT GCAGTAATTG GAGCAGCAGC TTTGACAGGG GCTGTCTCCC 
     2641 ACACAGTCTC CACAGCCGTC ATTTGCTTCG AATTAACCGG TCAGATTGCT CACATCCTGC 
     2701 CCATGATGGT GGCTGTTATC TTGGCCAACA TGGTGGCTCA GAGTCTGCAG CCCTCCCTCT 
     2761 ATGACAGCAT CATCCAGGTC AAGAAGCTTC CCTATCTGCC AGACCTTGGT TGGAACCAGC 
     2821 TCAGCAAATT TACAATTTTT GTTGAGGACA TCATGGTACG TGATGTGAAG TTCGTTTCAG 
     2881 CTTCTTGTAC ATATGGGGAA CTGAGAAACC TACTCCAGGC CACCACAGTC AAGACTTTAC 
     2941 CATTGGTTGA CTCCAAAGAT TCAATGATCC TGCTGGGCTC TGTGGAACGC TCAGAACTGC 
     3001 AGTCCCTCCT GCAGCGCCAC CTGTGTGCAG AGCGAAGGTT GAAGGCTGCC CAGGACATGG 
     3061 CTCGAAAGTT ATCAGAGCTG CCTTATAATG GCAAGGCTCA GCTGGCTGGG GACTGGCATC 
     3121 CTGGTGGTCG ACCTGAGTCC TTTGCCTTCG TAGATGAAGA TGAAGATGAG GACCTCTCCA 
     3181 GGAAGATGGA GCTTCCACTG ACTCCAGCTC CTCCCCCTCC TTCCCCTCCT CCTCCACCTT 
     3241 CCCAGTTTCC TATTGCTCCA TCAAACCCTG AAGAACCTAA TGGGCCACTG CCCAGCCATA 
     3301 AACAGCCACC TGAAGCCTCA GACTCTGCAG ATCAAAGATC ATCCACCTTC CAACGCCTGC 
     3361 TGCACTGCTT GCTAGGAAAA GCTCACTCCA AAAAGAAGAA AATAACACAG GATTCAACAG 
     3421 ATTTAGTGGA TAACATGTCA CCTGAAGAGA TTGAGGCCTG GGAGCGGGAG CAGCTGAGCC 
     3481 AGCCTGTGTG CTTTGATTGC TGCTGCATCG ACCAGTCTCC CTTCCAGCTG GTGGAGCAGA 
     3541 CAACCCTGCA CAAGACTCAT ACACTCTTCT CACTTCTTGG CCTCCATCTT GCCTATGTGA 
     3601 CCAGCATGGG GAAGCTCAGA GGTGTCTTGG CACTAGAAGA GCTACAGAAA GCTATTGAGG 
     3661 GCCACACCAA ATCTGGGGTG CAGCTTCGCC CTCCACTTGC CAGCTTCCGG AATACAACTT 
     3721 CAATCCGGAA GACTCCTGGG GGGCCACCCC CTCCTGCAGA GGGCTGGAAT GTACCTGAGG 
     3781 ATGGAGATGG GGCTCCTGGA AGAGAAGTCA TGGTTCCTAC CATGCCAGAG ACTCCTGTCC 
     3841 CACCACCATC TCCAGAGGCC CCTTCCTGCC TGGCCCCAGC CAGAGCGGAG GGTGAGCTGG 
     3901 AGGAACTGGA GATGGTGGGG AGCCTAGAGC CTGAGGAGGA GCTGGCTGAC ATCTTGCATG 
     3961 GCCCCAGTCT GCGGTCCACT GATGAGGAAG ATGAGGACGA GCTGATCCTG ACGCGTACGC 
     4021 GGCCGCTCGA GATGGAGAGC GACGAGAGCG GCCTGCCCGC CATGGAGATC GAGTGCCGCA 
     4081 TCACCGGCAC CCTGAACGGC GTGGAGTTCG AGCTGGTGGG CGGCGGAGAG GGCACCCCCG 
     4141 AGCAGGGCCG CATGACCAAC AAGATGAAGA GCACCAAAGG CGCCCTGACC TTCAGCCCCT 
     4201 ACCTGCTGAG CCACGTGATG GGCTACGGCT TCTACCACTT CGGCACCTAC CCCAGCGGCT 
     4261 ACGAGAACCC CTTCCTGCAC GCCATCAACA ACGGCGGCTA CACCAACACC CGCATCGAGA 
     4321 AGTACGAGGA CGGCGGCGTG CTGCACGTGA GCTTCAGCTA CCGCTACGAG GCCGGCCGCG 
     4381 TGATCGGCGA CTTCAAGGTG ATGGGCACCG GCTTCCCCGA GGACAGCGTG ATCTTCACCG 
     4441 ACAAGATCAT CCGCAGCAAC GCCACCGTGG AGCACCTGCA CCCCATGGGC GATAACGATC 
     4501 TGGATGGCAG CTTCACCCGC ACCTTCAGCC TGCGCGACGG CGGCTACTAC AGCTCCGTGG 
     4561 TGGACAGCCA CATGCACTTC AAGAGCGCCA TCCACCCCAG CATCCTGCAG AACGGGGGCC 
     4621 CCATGTTCGC CTTCCGCCGC GTGGAGGAGG ATCACAGCAA CACCGAGCTG GGCATCGTGG 
     4681 AGTACCAGCA CGCCTTCAAG ACCCCGGATG CAGATGCCGG TGAAGAAAGA GTTTAAACGG 
     4741 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4801 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4861 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4921 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4981 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5041 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5101 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5161 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5221 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5281 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5341 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5401 GGTACGCGGC CAGCTTGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 
     5461 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 
     5521 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5581 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 
     5641 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC 
     5701 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 
     5761 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 
     5821 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 
     5881 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 
     5941 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 
     6001 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 
     6061 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 
     6121 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 
     6181 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 
     6241 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 
     6301 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 
     6361 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 
     6421 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCCGA CCGCTATCAG 
     6481 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 
     6541 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 
     6601 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA 
     6661 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 
     6721 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 
     6781 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 
     6841 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 
     6901 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT 
     6961 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 
     7021 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 
     7081 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 
     7141 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 
     7201 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 
     7261 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 
     7321 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 
     7381 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 
     7441 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 
     7501 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 
     7561 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 
     7621 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 
     7681 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 
     7741 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 
     7801 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 
     7861 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC 
     7921 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 
     7981 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 
     8041 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 
     8101 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 
     8161 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 
     8221 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 
     8281 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 
     8341 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 
     8401 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 
     8461 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 
     8521 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 
     8581 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 
     8641 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 
     8701 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 
     8761 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 
     8821 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 
     8881 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 
     8941 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 
     9001 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 
     9061 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT 
     9121 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 
     9181 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 
     9241 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 
     9301 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 
     9361 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 
     9421 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT 
     9481 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 
     9541 TT
//