LOCUS       MG219052                9344 bp    DNA     circular  SYN 
DEFINITION  Pde2a (tGFP-tagged) - Mouse phosphodiesterase 2A cGMP-stimulated (Pde2a) transcript variant 3, (10ug)
ACCESSION   NM_001143849
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3825..4529 
                     /label="GFP"  
     misc_feature    4576..5161 
                     /label="PolyA signal"  
     misc_feature    7253..7898 
                     /label="ColE1"  
     misc_feature    5615..6409 
                     /label="Neo"  
     misc_feature    5246..5548 
                     /label="SV40 ori"  
     misc_feature    7913..8773 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3804..3810 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3804 
                     /label="Pde2a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCCTGGTG TTGCACCACA TCCTCATCGC TGTTGTCCAA TTCCTCAGGC 
     1081 GGGGCCAGCA GGTCTTCCTC AAGCCGGACG AGCCGCCGCC GCAGCCATGC GCCGACAGCC 
     1141 TGCAGGATGC TTTGCTGAGC CTAGGCGCCG TTATCGACAT TGCTGGCCTG CGACAGGCTG 
     1201 CCAGGGATGC CCTCTCAGCC GTGCTCCCCA AAGTGGAGAC TGTCTACACC TACCTGCTAG 
     1261 ATGGGGAGTC CAGACTGGTG TGTGAGGACC CCCCTCATGA GCTGCCACAG GAAGGAAAAA 
     1321 TTCGAGAAGC TGTGATCTCT CAGAAGCGGC TGAGTTGCAA TGGGCTGGGA CCTTCGGACC 
     1381 TACTGGGAAA GCCCTTGGCC AGGCTGGTGG CTCCACTGGC TCCTGACATG CAAGTGCTGG 
     1441 TCATACCCCT GCTGGACAAG GAGACTGGAA GTGTGGCAGC TGTCATCTTG GTGCACTGTG 
     1501 GCCAGCTGAG TGACAGTGAG GAACAGAGCT TGCAGGTGGT AGAGAAGCAC GCTCTGGTAG 
     1561 CCCTGCGGAG GGTGCAGGCC CTGCAGCAGC GCAGGCCTGA AGCTGTTCAG AACACGTCAG 
     1621 TGGATGCCTC TGAAGATCAA AAGGATGAGA AGGGGTACAC CGACCATGAC CGAAAGATCC 
     1681 TGCAACTGTG TGGGGAACTC TTTGACTTGG ATGCTACTTC TCTGCAGCTC AAAGTCCTTC 
     1741 AATATCTGCA GCAGGAGACA CAGGCCACTC ACTGCTGCCT CCTGCTGGTG TCGGAGGACA 
     1801 ATCTACAGCT TTCCTGCAAG GTCATTGGAG ACAAAGTGCT GGGAGAAGAG GTCAGCTTTC 
     1861 CATTGACCAT GGGACGTCTG GGGCAGGTGG TGGAAGACAA GCAGTGTATC CAGTTGAAGG 
     1921 ACCTAACCTC TGACGATGTG CAACAGCTGC AAAACATGTT GGGTTGTGAG CTGCAGGCTA 
     1981 TGCTGTGTGT CCCTGTCATC AGCCGAGCCA CTGACCAGGT GGTGGCCCTG GCTTGCGCCT 
     2041 TCAACAAGCT TGGAGGAGAT TTCTTCACAG ATGAGGATGA ACACGTGATC CAACACTGCT 
     2101 TCCACTACAC AGGCACGGTG CTCACCAGCA CCTTGGCCTT CCAGAAGGAG CAGAAGCTCA 
     2161 AGTGTGAGTG CCAGGCTCTT CTCCAAGTGG CAAAGAACCT CTTCACCCAC CTGGATGATG 
     2221 TCTCTGTCCT GCTACAGGAG ATCATCACGG AAGCCAGAAA CCTCAGCAAC GCAGAGATCT 
     2281 GCTCGGTGTT CCTGCTGGAT CAGAACGAGC TGGTGGCCAA GGTGTTCGAT GGTGGCGTTG 
     2341 TGGACGATGA GAGTTATGAG ATCCGCATCC CGGCGGACCA AGGCATCGCG GGCCACGTGG 
     2401 CGACTACGGG CCAGATCCTG AACATCCCAG ATGCATATGC CCATCCGCTT TTCTATCGCG 
     2461 GCGTAGATGA CAGCACTGGC TTCCGCACGC GCAACATTCT CTGCTTCCCT ATCAAGAACG 
     2521 AGAACCAGGA GGTCATTGGT GTGGCTGAGC TAGTGAACAA GATCAATGGG CCATGGTTCA 
     2581 GCAAGTTTGA TGAGGACCTG GCCACAGCCT TCTCCATCTA CTGTGGCATC AGCATCGCCC 
     2641 ACTCTCTCCT ATACAAAAAG GTGAATGAAG CCCAATACCG CAGCCACCTG GCCAATGAGA 
     2701 TGATGATGTA TCATATGAAG GTCTCTGATG ATGAATACAC CAAGCTTCTC CACGATGGCA 
     2761 TCCAACCTGT GGCCGCCATT GACTCCAACT TTGCCAACTT TACCTACACG CCTCGGTCTC 
     2821 TGCCTGAGGA CGACACTTCT ATGGCCATCC TGAGCATGCT GCAAGACATG AACTTCATCA 
     2881 ATAACTACAA AATTGACTGC CCAACTCTGG CCCGATTCTG CCTGATGGTG AAGAAAGGCT 
     2941 ACCGGGATCC ACCCTACCAC AACTGGATGC ACGCCTTCTC TGTCTCTCAT TTTTGCTACC 
     3001 TGCTCTACAA GAATCTGGAG CTCTCCAACT ACCTCGAGGA CATCGAGATC TTTGCATTGT 
     3061 TTATTTCCTG CATGTGTCAT GACCTGGACC ACAGAGGCAC AAACAACTCC TTCCAGGTGG 
     3121 CCTCGAAATC TGTGCTGGCC GCACTCTACA GCTCAGAGGG CTCTGTCATG GAGAGGCACC 
     3181 ACTTTGCTCA AGCCATTGCT ATCCTCAACA CCCACGGCTG CAATATCTTT GACCACTTCT 
     3241 CTCGGAAGGA CTATCAGCGC ATGCTGGACC TGATGAGGGA CATCATCTTG GCTACAGACC 
     3301 TGGCACACCA CCTCCGCATC TTCAAGGACC TGCAGAAGAT GGCTGAAGTG GGTTATGACC 
     3361 GAAACAACAG GCAACACCAC AGGCTTCTTC TGTGCCTCCT CATGACCTCC TGTGACCTCT 
     3421 CTGACCAGAC AAAGGGCTGG AAGACCACCA GAAAGATTGC AGAGCTGATC TACAAAGAGT 
     3481 TCTTCTCCCA GGGAGACTTG GAGAAGGCCA TGGGCAACCG ACCGATGGAG ATGATGGACC 
     3541 GTGAGAAGGC CTACATCCCT GAGCTTCAGA TCAGCTTTAT GGAGCACATT GCCATGCCTA 
     3601 TCTACAAGCT TTTACAAGAC CTGTTCCCCA AGGCGGCAGA GCTGTATGAA CGTGTGGCCT 
     3661 CCAACCGTGA GCACTGGACC AAGGTGTCCC ACAAGTTCAC CATCCGAGGC CTCCCCAGTA 
     3721 ACAACTCGCT GGATTTCCTG GACGAGGAAT ACGAGGTCCC CGATTTGGAC GGCACCAGAG 
     3781 CTCCTGTCAA TGGCTGCTGC AGCCTCGAGG GCACGCGTAC GCGGCCGCTC GAGATGGAGA 
     3841 GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG 
     3901 GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA 
     3961 ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA 
     4021 TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC 
     4081 ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG 
     4141 TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG 
     4201 TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA 
     4261 ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC 
     4321 GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT 
     4381 TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC 
     4441 GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA 
     4501 AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4561 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4621 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4681 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4741 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4801 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4861 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4921 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4981 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5041 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5101 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5161 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG 
     5221 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5281 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5341 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 
     5401 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
     5461 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 
     5521 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 
     5581 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 
     5641 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 
     5701 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 
     5761 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 
     5821 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 
     5881 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 
     5941 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 
     6001 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 
     6061 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 
     6121 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 
     6181 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 
     6241 TTCATCGACT GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC 
     6301 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 
     6361 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 
     6421 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 
     6481 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 
     6541 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 
     6601 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 
     6661 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 
     6721 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 
     6781 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 
     6841 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 
     6901 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 
     6961 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
     7021 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     7081 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     7141 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     7201 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     7261 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     7321 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     7381 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     7441 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     7501 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     7561 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     7621 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     7681 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     7741 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     7801 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     7861 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     7921 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     7981 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     8041 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     8101 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     8161 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     8221 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     8281 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     8341 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     8401 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     8461 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     8521 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     8581 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     8641 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     8701 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     8761 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     8821 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     8881 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 
     8941 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 
     9001 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 
     9061 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 
     9121 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 
     9181 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 
     9241 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 
     9301 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//