LOCUS       MG218890                8981 bp    DNA     circular  SYN 
DEFINITION  Plcd4 (tGFP-tagged) - Mouse phospholipase C delta 4 (Plcd4) transcript variant 2, (10ug)
ACCESSION   NM_148937
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3462..4166 
                     /label="GFP"  
     misc_feature    4213..4798 
                     /label="PolyA signal"  
     misc_feature    6890..7535 
                     /label="ColE1"  
     misc_feature    5252..6046 
                     /label="Neo"  
     misc_feature    4883..5185 
                     /label="SV40 ori"  
     misc_feature    7550..8410 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3441..3447 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3441 
                     /label="Plcd4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACATCTCAG ATTCAAGACC TACTAGCCAC TGATCAGGAC CTGCTGCTGA 
     1081 TGCAAGAAGG CACAATGATG CGCAAGGTGA GGACCAAAAG CTGGAAGAAG CTAAGATACT 
     1141 TCAGACTTCA GAATGACGGC ATGACAGTCT GGCATGGAAG TCAACCAGAA AGCATGCCCA 
     1201 AGCCCACTTT TTCGATCTCT GATGTGGAGA GGATACGTAA GGGGCAGGAT TCTGAGTTGT 
     1261 TGCGCTATCT GGTGGAGGAG TTTCCCCTGG AGCAAGGGTT CACCGTTGTC TTTCATGGGC 
     1321 GCCGCCCCAA CCTGGACCTA GTGGCCAACA GTGTTGAAGA GGCCCAGATT TGGATGCGAG 
     1381 GACTCCAGCT GTTGGTGGAT CTTGTTGCCA GCATGGACCA CCAGGAGCAA ATGGATCAAA 
     1441 TGTTGAATGA GTGGTTCCAG CAAGCAGACA GAAACCAGGA TGGCCGGATG AGTTTCAGAG 
     1501 AAGCTCAACG GCTGCTGCTT CTGATGAACG TGGAAATGGA TGAAGAGTAC GCCTTCAGTC 
     1561 TTTTCCAGGA GGCAGATGTT ACCCAGTCTG ATGATCTGGG GTCGGAAGAA TTTGTACAGT 
     1621 TCTATAAGGC CCTGACTAAG CGCACTGAGA TTGAAGAAAT ATTTGAGGAC TTCTCATCTG 
     1681 ACAAACAGAA GCTGACCTTG CTGGAATTTG TGGATTTCCT TAGAAAGGAA CAGAAAGAAA 
     1741 AAGACCATGC TCCTGACCTC GCTCTGGAAC TCATTGACCG CTATGAGCCT TCTGAGAATG 
     1801 GCCGATTGCT GCATGTGCTG AGCAAGGATG GCTTCCTCAA GTACCTCTGC TCGAAGGATG 
     1861 GAAACATCTT CAACAGTGAC TGCCTCCCTA TCTACCAGGA TATGACTCAG CCTCTGAGCC 
     1921 ACTACTACAT TAACTCTTCT CACAACACCT ACCTAGTGGG GGACCAGCTT TGCGGCCAGA 
     1981 GCAGCGTTGA AGGATACATA CGGGCCTTGA AACGGGGGTG CCGCTGTGTG GAGGTGGACA 
     2041 CGTGGGATGG ACCTGATGGA GAGCCTGTTG TTTATCATGG GCATACCCTG ACCTCTCGCA 
     2101 TCCTATTCAA AGATGTGTTG GCAACTCTCG CGCAATATGC CTTCCAGTCA TCAGACTACC 
     2161 CTCTCATCTT GTCTCTGGAA AACCACTGCA CCTGGGAACA GCAGAGGACC ATGGCACACC 
     2221 ATCTGACCGA GATCCTGGGA GAGCAGCTGC TGAGGAACAC CCTAGAGGGA TTGCTGGTTG 
     2281 ACAGCATGCC CTCGCCAGAG CAGCTTCGGG GGAAGATCTT GGTGAAAGGA AAGAAGTTAA 
     2341 GAACAATTGA AGTTGACAAG GAAGAAGAAG AAGAAGAAGA AGAAGAAGAG CTGGAGAAAG 
     2401 ATGAGGGGCC AGATCTGGAC CCAGCCTCCC CTGAGTTGGA CACTCAACCT CAGCCTGAGA 
     2461 CCCAGGGGCA GGCCGCTGGG AACAAGAAGG AGAGGAAGAA GAAGGTTATG AAGTGTCCGA 
     2521 TGTCTTGTCT GCTTATCTGT GGTCATGTTA TGGCCCAGGC TCCTAGTTCC ATTCCTGAGT 
     2581 CCATTCTCTT GTCAAAGCAG TTTCTTCTCC TGAGTTCCAC AACCATCATG TGCCCAGACC 
     2641 TATCTGCCCT GGTTGTCTAC TTGAGGACTG TCCCCTTCTG CAGCTTCACT CATTCCAAAG 
     2701 AAAACTACCA CATCTATGAC ATTTCATCCT TTTCTGAGTC CAAGGCCAAG AACCTCATCA 
     2761 AGGAGGCAGG CAACGAGTTT GTACAGCACA ATGCCAGACA GTTATGCCGC GTGTATCCCA 
     2821 GTGGTCTGAG GACAGATTCA TCCAACTTTA ACCCCCAGGA ACACTGGAAT GTAGGCTGCC 
     2881 AGATGGTGGC TATGAATATG CAGACTGCAG GAAGTGCAAT GGATATCTGT GATGGGCTCT 
     2941 TCCGCCAGAA TGGTGGCTCT GGCTATGTGC TGAAGCCAGA ATTCCTGAGA GATACCCAGA 
     3001 GTTCATTCAA TCCTGAGAGG CCAATTAGTC TTTATAAGGC CCAGATCCTT GTAGTCCAGG 
     3061 TGATCAGTGG CCAACAACTC CCCAAAGTGG ATAAAACCAA AGAGACAACC GTTGTGGATC 
     3121 CACTGGTGAA AGTAGAGCTC TATGGTGTTC CTGAAGACAC AAAGGAGCAA GAGACCAGCC 
     3181 ATGTGGAGAA CAATGGTATT AATCCATACT GGGGGGAGAC TTTCTATTTC CGGCTCCAGG 
     3241 TACCTGAACT TGCTATGTTG CGTTTTGTGG TAAAGGATTA TAGCAGGAAA TCCCGAAATA 
     3301 ACTTTATTGG CCAATACACC CTACCTTGGA CCTGTATGAA ACAAGGCTAC CGACATGTAT 
     3361 CCTTGCTCTC CAGAGATGGC ACTAGCCTCA ATCCAGCTTC CATCTTTGTA TACACCTGCA 
     3421 TGCAGGAAGA CCTGGATATG GATGAGCCCA CGCGTACGCG GCCGCTCGAG ATGGAGAGCG 
     3481 ACGAGAGCGG CCTGCCCGCC ATGGAGATCG AGTGCCGCAT CACCGGCACC CTGAACGGCG 
     3541 TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG GCACCCCCGA GCAGGGCCGC ATGACCAACA 
     3601 AGATGAAGAG CACCAAAGGC GCCCTGACCT TCAGCCCCTA CCTGCTGAGC CACGTGATGG 
     3661 GCTACGGCTT CTACCACTTC GGCACCTACC CCAGCGGCTA CGAGAACCCC TTCCTGCACG 
     3721 CCATCAACAA CGGCGGCTAC ACCAACACCC GCATCGAGAA GTACGAGGAC GGCGGCGTGC 
     3781 TGCACGTGAG CTTCAGCTAC CGCTACGAGG CCGGCCGCGT GATCGGCGAC TTCAAGGTGA 
     3841 TGGGCACCGG CTTCCCCGAG GACAGCGTGA TCTTCACCGA CAAGATCATC CGCAGCAACG 
     3901 CCACCGTGGA GCACCTGCAC CCCATGGGCG ATAACGATCT GGATGGCAGC TTCACCCGCA 
     3961 CCTTCAGCCT GCGCGACGGC GGCTACTACA GCTCCGTGGT GGACAGCCAC ATGCACTTCA 
     4021 AGAGCGCCAT CCACCCCAGC ATCCTGCAGA ACGGGGGCCC CATGTTCGCC TTCCGCCGCG 
     4081 TGGAGGAGGA TCACAGCAAC ACCGAGCTGG GCATCGTGGA GTACCAGCAC GCCTTCAAGA 
     4141 CCCCGGATGC AGATGCCGGT GAAGAAAGAG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4201 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4261 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4321 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4381 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4441 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4501 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4561 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4621 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4681 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4741 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4801 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCTTGGCTG 
     4861 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     4921 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     4981 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 
     5041 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 
     5101 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG 
     5161 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 
     5221 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 
     5281 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 
     5341 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 
     5401 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 
     5461 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 
     5521 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 
     5581 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 
     5641 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 
     5701 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 
     5761 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 
     5821 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 
     5881 ATCGACTGTG GCCGGCTGGG TGTGGCCGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 
     5941 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 
     6001 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 
     6061 GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG 
     6121 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 
     6181 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 
     6241 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 
     6301 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 
     6361 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 
     6421 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 
     6481 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 
     6541 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 
     6601 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 
     6661 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 
     6721 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 
     6781 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 
     6841 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 
     6901 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 
     6961 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 
     7021 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 
     7081 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 
     7141 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 
     7201 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 
     7261 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 
     7321 AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 
     7381 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 
     7441 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 
     7501 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 
     7561 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 
     7621 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 
     7681 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 
     7741 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 
     7801 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 
     7861 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 
     7921 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 
     7981 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 
     8041 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 
     8101 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 
     8161 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 
     8221 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 
     8281 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 
     8341 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 
     8401 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 
     8461 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 
     8521 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 
     8581 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 
     8641 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 
     8701 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 
     8761 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 
     8821 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 
     8881 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 
     8941 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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