LOCUS       MG218857                9116 bp    DNA     circular  SYN 
DEFINITION  Polk (tGFP-tagged) - Mouse polymerase (DNA directed) kappa (Polk), (10ug)
ACCESSION   NM_012048
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3597..4301 
                     /label="GFP"  
     misc_feature    4348..4933 
                     /label="PolyA signal"  
     misc_feature    7025..7670 
                     /label="ColE1"  
     misc_feature    5387..6181 
                     /label="Neo"  
     misc_feature    5018..5320 
                     /label="SV40 ori"  
     misc_feature    7685..8545 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3576..3582 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3576 
                     /label="Polk"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATAACACA AAGGAAAAGG ACAACTTCAA AGACGACCTC CTGCTCCGCA 
     1081 TGGGACTAAA CGATAACAAA GCAGGCATGG AAGGGTTGGA TAAGGAGAAA ATTAACAAAA 
     1141 TTATCATGGA AGCCACAAAG GGGTCCAGAT TTTATGGAAA TGAGCTCAAG AAGGAAAAGC 
     1201 AAGTCAATCA ACGGATTGAA AATATGATGC AACAAAAAGC TCAAATTACC AGCCAGCAAC 
     1261 TAAGGAAAGC TCAATTACAG GTTGACAAAT TTGCAATGGA GTTAGAACGG AACCGGAATT 
     1321 TGAACAATAC CATAGTTCAT GTTGACATGG ACGCTTTCTA TGCAGCTGTG GAAATGAGGG 
     1381 ACAACCCGGA ACTGAAGGAT AAACCCATTG CTGTAGGATC CATGAGCATG TTGGCTACTT 
     1441 CGAATTACCA TGCAAGGAGG TTTGGTGTCC GTGCAGCCAT GCCAGGATTT ATTGCTAAGA 
     1501 GGCTCTGCCC ACAACTTATT ATAGTGCCCC CAAACTTTGA CAAATATAGA GCTGTGAGTA 
     1561 AGGAGGTTAA GGAGATACTT GCTGAATATG ATCCCAATTT TATGGCCATG AGTCTGGACG 
     1621 AAGCCTACTT GAATATAACA CAGCACTTGC AGGAAAGGCA AGATTGGCCT GAGGACAAAA 
     1681 GAAGATACTT CATCAAGATG GGAAACTACT TAAAAATCGA CACACCCAGA CAGGAAGCTA 
     1741 ACGAGCTGAC TGAGTATGAG CGGTCCATCT CCCCGCTGCT TTTTGAAGAT AGTCCTCCTG 
     1801 ATTTGCAACC CCAAGGAAGT CCTTTCCAAC TGAACTCTGA AGAACAAAAC AATCCTCAAA 
     1861 TAGCCCAAAA TTCAGTTGTT TTTGGAACAT CAGCTGAGGA AGTGGTAAAG GAAATTCGCT 
     1921 TCAGAATTGA ACAAAAAACA ACGCTGACAG CCAGCGCAGG CATCGCCCCC AATACAATGT 
     1981 TAGCAAAAGT GTGCAGTGAT AAGAATAAGC CAAACGGACA GTACCAGATC CTTCCCAGCA 
     2041 GGAGCGCGGT GATGGACTTC ATCAAGGACC TGCCTATTAG AAAGGTTTCT GGGATAGGAA 
     2101 AAGTTACAGA GAAAATGTTA ATGGCTCTCG GGATTGTTAC TTGCACAGAA CTCTACCAAC 
     2161 AGAGAGCGTT GCTGTCTCTC CTTTTCTCTG AAACCTCTTG GCATTATTTT CTTCACATCG 
     2221 CGCTGGGTCT AGGTTCAACA GACCTGGCAA GGGATGGAGA AAGGAAAAGC ATGAGTGTTG 
     2281 AAAGGACATT CAGTGAGATA AGTAAGACAG AGGAACAGTA CAGCCTGTGC CAAGAACTGT 
     2341 GCGCTGAGCT CGCCCACGAC CTCCAGAAGG AAGGACTTAA GGGAAGAACC GTCACCATTA 
     2401 AGCTGAAGAA CGTGAATTTT GAAGTAAAAA CTCGTGCATC TACCGTTCCG GCCGCCATTT 
     2461 CTACTGCAGA GGAAATATTT GCCATTGCCA AGGAGCTGCT AAGGACAGAA GTTAATGTGG 
     2521 GTTCTCCACA CCCCCTGCGG TTAAGACTGA TGGGTGTCCG AATGTCTACT TTTTCCAGTG 
     2581 AAGATGACAG GAAACACCAA CAAAGGAGCA TCATTGGTTT CTTACAAGCT GGAAACCAAG 
     2641 CTTTGTCATC TACTGGGGAT AGTCTAGACA AAACTGACAA AACTGAGCTT GCAAAGCCCT 
     2701 TAGAAATGTC TCATAAGAAG AGTTTCTTTG ATAAAAAGCG ATCAGAAAGA ATCTCCAACT 
     2761 GTCAAGACAC ATCCAGATGT AAAACTGCGG GTCAGCAAGC TTTACAGATC TTGGAACCAT 
     2821 CCCAAGCATT AAAGAAGCTG AGCGAGAGTT TTGAAACATC AGAGAATTCA AATGACTGTC 
     2881 AGACATTTAT ATGTCCAGTT TGCTTTAGGG AGCAAGAAGG TGTCAGTCTG GAAGCCTTTA 
     2941 ATGAACATGT AGATGAGTGT CTTGATGGAC CGTCAACCAG TGAGAACTCA AAAATATCCT 
     3001 GTTACTCACA TGCTTCCTCT GCAGACATTG GTCAGAAGGA AGATGTACAC CCCTCTATTC 
     3061 CACTGTGTGA GAAACGGGGG CATGAAAATG GAGAGATCAC TTTAGTAGAT GGTGTAGATC 
     3121 TAACAGGGAC GGAAGACAGA TCATTGAAAG CAGCAAGGAT GGACACTCTA GAGAATAATC 
     3181 GCAGCAAAGA GGAATGTCCT GATATTCCAG ACAAGTCTTG TCCTATATCA CTGGAAAATG 
     3241 AAACCATCAG TACATTAAGT AGGCAAGACT CTGTCCAGCC TTGTACAGAT GAGGTAGTAA 
     3301 CAGGACGAGC TCTAGTGTGT CCTGTTTGTA ACCTAGAACA AGAGACTTCT GATCTTACCC 
     3361 TCTTCAACAT ACATGTGGAT ATTTGCTTAA ATAAAGGTAT TATCCAAGAA CTGAGAAATA 
     3421 GTGAAGGTAA TTCAGTTAAA CAACCCAAAG AAAGCTCGAG AAGTACTGAC AGACTTCAGA 
     3481 AGGCTTCAGG AAGGACCAAA AGGCCAGGAA CGAAGACAAA GAGCTCAACT TTGAAGAAAA 
     3541 CAAAGCCCCG AGATCCCAGA CACACCCTTG ATGGATTTTT TAAAACGCGT ACGCGGCCGC 
     3601 TCGAGATGGA GAGCGACGAG AGCGGCCTGC CCGCCATGGA GATCGAGTGC CGCATCACCG 
     3661 GCACCCTGAA CGGCGTGGAG TTCGAGCTGG TGGGCGGCGG AGAGGGCACC CCCGAGCAGG 
     3721 GCCGCATGAC CAACAAGATG AAGAGCACCA AAGGCGCCCT GACCTTCAGC CCCTACCTGC 
     3781 TGAGCCACGT GATGGGCTAC GGCTTCTACC ACTTCGGCAC CTACCCCAGC GGCTACGAGA 
     3841 ACCCCTTCCT GCACGCCATC AACAACGGCG GCTACACCAA CACCCGCATC GAGAAGTACG 
     3901 AGGACGGCGG CGTGCTGCAC GTGAGCTTCA GCTACCGCTA CGAGGCCGGC CGCGTGATCG 
     3961 GCGACTTCAA GGTGATGGGC ACCGGCTTCC CCGAGGACAG CGTGATCTTC ACCGACAAGA 
     4021 TCATCCGCAG CAACGCCACC GTGGAGCACC TGCACCCCAT GGGCGATAAC GATCTGGATG 
     4081 GCAGCTTCAC CCGCACCTTC AGCCTGCGCG ACGGCGGCTA CTACAGCTCC GTGGTGGACA 
     4141 GCCACATGCA CTTCAAGAGC GCCATCCACC CCAGCATCCT GCAGAACGGG GGCCCCATGT 
     4201 TCGCCTTCCG CCGCGTGGAG GAGGATCACA GCAACACCGA GCTGGGCATC GTGGAGTACC 
     4261 AGCACGCCTT CAAGACCCCG GATGCAGATG CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC 
     4321 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4381 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4441 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4501 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4561 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4621 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4681 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4741 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4801 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4861 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4921 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4981 CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5041 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC 
     5101 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA 
     5161 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG 
     5221 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG 
     5281 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG 
     5341 GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 
     5401 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 
     5461 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 
     5521 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 
     5581 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 
     5641 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 
     5701 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 
     5761 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 
     5821 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 
     5881 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC 
     5941 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 
     6001 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CCGACCGCTA TCAGGACATA 
     6061 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 
     6121 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 
     6181 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC 
     6241 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 
     6301 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 
     6361 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 
     6421 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 
     6481 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT 
     6541 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 
     6601 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 
     6661 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 
     6721 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 
     6781 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 
     6841 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 
     6901 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 
     6961 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 
     7021 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 
     7081 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 
     7141 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 
     7201 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 
     7261 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 
     7321 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA 
     7381 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 
     7441 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA 
     7501 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 
     7561 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 
     7621 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 
     7681 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 
     7741 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 
     7801 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 
     7861 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 
     7921 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 
     7981 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 
     8041 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 
     8101 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 
     8161 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 
     8221 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 
     8281 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 
     8341 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 
     8401 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 
     8461 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 
     8521 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 
     8581 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 
     8641 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG 
     8701 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 
     8761 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 
     8821 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 
     8881 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 
     8941 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 
     9001 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA 
     9061 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//