LOCUS       MG218680                8975 bp    DNA     circular  SYN 
DEFINITION  Arhgap6 (tGFP-tagged) - Mouse Rho GTPase activating protein 6 (Arhgap6) transcript variant 2, (10ug)
ACCESSION   NM_178754
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3456..4160 
                     /label="GFP"  
     misc_feature    4207..4792 
                     /label="PolyA signal"  
     misc_feature    6884..7529 
                     /label="ColE1"  
     misc_feature    5246..6040 
                     /label="Neo"  
     misc_feature    4877..5179 
                     /label="SV40 ori"  
     misc_feature    7544..8404 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3435..3441 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3435 
                     /label="Arhgap6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGGACCCG TCCTACAGCG AGAAACCCCG TCTGCACTAC GCGGGTGACT 
     1081 TCACCTGGAA CAGCATGTCT GGTCGTAGCG TGCGCCTGAG GTCAGTCCCC ATCCAGAGCC 
     1141 TCTCAGAGCT GGAGCGGGCA CGACTGCAGG AAGTGGCTTT TTATCAGTTG CAGCAGGACT 
     1201 GTGACCTGGG CTGTCAGATC ACCATCCCCA AAGATGGACA AAAGAGAAAG AAATCTTTGA 
     1261 GAAAGAAACT GGATTCACTA GGGAAGGAAA AGAACAAAGA CAAAGAATTC ATCCCACAGG 
     1321 CATTTGGAAT GCCCTTATCC CAAGTCATTG CTAATGACCG GGCATATAAA CTGAAGCAAG 
     1381 ACTTGCAGAG GGAGGAGCAG AAGGATGCAT CATCGGATTT TGTGTCTTCC CTCCTCCCAT 
     1441 TTGGGAATAA AAAACAAAAC AAAGAACTCT CAAGCAGTAA CTCATCTCTC AGCTCAACCT 
     1501 CAGAAACACC AAATGAGTCT ACATCACCGA ATACTCCAGA ACCAGCTCCT CGGGCCAGGA 
     1561 GAAGGGGCGC CATGTCCGTG GATTCCATCA CTGATCTGGA TGACAACCAG TCTCGACTCC 
     1621 TAGAAGCTTT ACAACTCTCC TTGCCTGCTG AGGCTCAGAG TAAAAAAGAA AAGGCCAGAG 
     1681 ATAAGAAGCT GAGTCTGAAT CCTATTTACA GGCAGGTCCC CAGGCTGGTG GACAGCTGCT 
     1741 GTCAACATCT GGAAAAACAT GGCCTCCAGA CAGTGGGGAT ATTCCGAGTT GGAAGCTCAA 
     1801 AGAAGAGAGT AAGACAATTG CGTGAAGAAT TTGACCGTGG GGTTGATGTC TGTCTGGAAG 
     1861 AGGAGCATAG TGTTCACGAT GTGGCAGCCT TGTTAAAGGA GTTCCTTAGA GACATGCCTG 
     1921 ACCCCCTTCT CACAAGGGAG CTATACACTG CATTTATCAA CACTCTCCTG TTGGAGCCTG 
     1981 AGGAACAACT GGGCACCTTG CAACTCCTCA TTTACCTTCT ACCTCCCTGC AACTGCGACA 
     2041 CCCTCCACCG CCTCCTACAG TTCCTCTCCA TTGTGGCCAG GCATGCTGAT GATAATGTCA 
     2101 GCAAAGATGG ACAAGAGGTT ACTGGGAACA AAATGACATC TCTGAACTTA GCCACTATAT 
     2161 TTGGACCCAA CCTGCTCCAC AAGCAGAAGT CATCAGACAA AGAATATTCT GTTCAGAGCT 
     2221 CAGCCAGAGC TGAGGAGAGC ACAGCCATCA TAGCTGTGGT ACAGAAGATG ATTGAAAATT 
     2281 ATGAAGCCTT GTTCATGGTT CCCCCAGATC TCCAGAATGA AGTGCTGATC AGCCTTCTAG 
     2341 AGACAGATCC AGATGTTGTG GACTACTTGC TCAGAAGAAA GGCTTCCCAA TCCTCGAGCC 
     2401 CTGACATACT TCAGACGGAA GTTTCCTTTT CCATGGGAGG GAGGCATTCA TCTACAGATT 
     2461 CCAACAAAGC CTCCAGTGGA GACATCTCCC CTTATGACAA CAACTCCCCA GTATTGTCTG 
     2521 AGCGCTCCCT GCTGGCTATG CAAGAGGACA GGGCCCGGGG GGGCTCGGAG AAGCTTTATA 
     2581 AAGTGCCAGA GCAGTATACA CTGGTGGGCC ACTTGTCATC GCCAAAGTCA AAGTCAAGAG 
     2641 AAAGTTCTCC TGGACCAAGG CTTGGAAAAG AAATGTCAGA GGAGCCTTTC AATATCTGGG 
     2701 GAACTTGGCA TTCAACATTA AAAAGTGGAT CCAAAGACCC AGGAATGACA GGCTCTTATG 
     2761 GCGACATTTT TGAAAGCAGC TCCCTCCGAC CGAGGCCTTG TTCTCTTTCT CAAGGGAACC 
     2821 TTTCCCTGAA CTGGCCTCGG TGTCAAGGGA GCCCGACAGG GCTGGACAGT GGCACTCAGG 
     2881 TAATTCGGAG GACTCAGACG GCGGCCACCG TGGAGCAGTG CAGTGTCCAC CTTCCGGTGT 
     2941 CACGTGTCTG CAGCACTCCC CACATCCAGG ACGGCAGCAG GGGGACCAGG CGGCCTGCAG 
     3001 CCAGCTCTGA TCCATTTTTG TCCCTAAACA GCACAGAAGA TCTAGCTGAG GGCAAGGAGG 
     3061 ATGTTGCCTG GCTGCAAAGC CAGGCCCGAC CTGTGTACCA GAGACCTCAG GAGAGTGGAA 
     3121 AAGATGACAG GCGCCCCCCT CCTCCTTACC CGGGGTCAGG GAAGCCTGCC ACAACCTCTG 
     3181 CCCAGCTGCC ACTAGAGCCT CCCCTGTGGA GGCTCCAGAG GCATGAAGAA GGTTCAGAAA 
     3241 CAGCTGTGGA AGGAGGCCAG CAGGCCTCAG GGGAGCATCA GACCAGGCCA AAAAAACTGA 
     3301 GCAGCGCCTA CTCCCTCTCA GCCAGCGAGC AGGACAAACA GAACTTAGGG GAAGCCAGCT 
     3361 GGCTCGACTG GCAGCGAGAG CGGTGGCAGA TCTGGGAGCT TCTATCAACT GATAACCCCG 
     3421 ACGCCCTCCC GGAAACCCTA GTAACGCGTA CGCGGCCGCT CGAGATGGAG AGCGACGAGA 
     3481 GCGGCCTGCC CGCCATGGAG ATCGAGTGCC GCATCACCGG CACCCTGAAC GGCGTGGAGT 
     3541 TCGAGCTGGT GGGCGGCGGA GAGGGCACCC CCGAGCAGGG CCGCATGACC AACAAGATGA 
     3601 AGAGCACCAA AGGCGCCCTG ACCTTCAGCC CCTACCTGCT GAGCCACGTG ATGGGCTACG 
     3661 GCTTCTACCA CTTCGGCACC TACCCCAGCG GCTACGAGAA CCCCTTCCTG CACGCCATCA 
     3721 ACAACGGCGG CTACACCAAC ACCCGCATCG AGAAGTACGA GGACGGCGGC GTGCTGCACG 
     3781 TGAGCTTCAG CTACCGCTAC GAGGCCGGCC GCGTGATCGG CGACTTCAAG GTGATGGGCA 
     3841 CCGGCTTCCC CGAGGACAGC GTGATCTTCA CCGACAAGAT CATCCGCAGC AACGCCACCG 
     3901 TGGAGCACCT GCACCCCATG GGCGATAACG ATCTGGATGG CAGCTTCACC CGCACCTTCA 
     3961 GCCTGCGCGA CGGCGGCTAC TACAGCTCCG TGGTGGACAG CCACATGCAC TTCAAGAGCG 
     4021 CCATCCACCC CAGCATCCTG CAGAACGGGG GCCCCATGTT CGCCTTCCGC CGCGTGGAGG 
     4081 AGGATCACAG CAACACCGAG CTGGGCATCG TGGAGTACCA GCACGCCTTC AAGACCCCGG 
     4141 ATGCAGATGC CGGTGAAGAA AGAGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4201 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4261 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4321 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4381 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4441 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4501 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4561 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4621 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4681 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4741 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4801 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCTTG GCTGTGGAAT 
     4861 GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     4921 ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     4981 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC 
     5041 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT 
     5101 TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA 
     5161 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC 
     5221 GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC 
     5281 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA 
     5341 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT 
     5401 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG 
     5461 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA 
     5521 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 
     5581 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG 
     5641 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG 
     5701 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC 
     5761 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT 
     5821 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC 
     5881 TGTGGCCGGC TGGGTGTGGC CGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT 
     5941 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT 
     6001 CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC 
     6061 TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA 
     6121 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA 
     6181 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG 
     6241 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT 
     6301 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC 
     6361 CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT 
     6421 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG 
     6481 GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT 
     6541 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT 
     6601 TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC 
     6661 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG 
     6721 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG 
     6781 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 
     6841 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG 
     6901 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 
     6961 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG 
     7021 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT 
     7081 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 
     7141 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT 
     7201 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC 
     7261 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA 
     7321 CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 
     7381 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 
     7441 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 
     7501 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 
     7561 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 
     7621 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 
     7681 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 
     7741 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 
     7801 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 
     7861 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 
     7921 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 
     7981 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 
     8041 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 
     8101 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 
     8161 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 
     8221 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 
     8281 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 
     8341 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 
     8401 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 
     8461 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 
     8521 CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 
     8581 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 
     8641 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 
     8701 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 
     8761 AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 
     8821 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 
     8881 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 
     8941 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//