LOCUS       MG218623                9284 bp    DNA     circular  SYN 
DEFINITION  Gen1 (tGFP-tagged) - Mouse Gen homolog 1 endonuclease (Drosophila) (Gen1), (10ug)
ACCESSION   NM_177331
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3765..4469 
                     /label="GFP"  
     misc_feature    4516..5101 
                     /label="PolyA signal"  
     misc_feature    7193..7838 
                     /label="ColE1"  
     misc_feature    5555..6349 
                     /label="Neo"  
     misc_feature    5186..5488 
                     /label="SV40 ori"  
     misc_feature    7853..8713 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3744..3750 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3744 
                     /label="Gen1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAGTGAAT GACTTATGGC AAATTCTAGA GCCTGTAAAG CAACATATCC 
     1081 ACTTGCAAGA TCTTAGTGGG AAAACCATTG CCGTTGATTT GAGTCTCTGG GTATGCGAGG 
     1141 CACAGACAGT GAAGAAAATG ATAGGAACTG TCAAGAAGCC CCACCTCAGG AACCTATTTT 
     1201 TTCGTATTTC ATATTTAACT CAAATGAATG TAAAACTGGT GTTTGTAATG GAAGGGGAGC 
     1261 CACCAATGCT GAAAGCTGAT GTCATAAGCA AGAGGACTCA GACTCGTTAC GGGCCTTCTG 
     1321 GAAAATCAAG GTCTCAGAAA ACAGGGAGAT CACATTTTAA GTCAGTCTTA CGAGAGTGCC 
     1381 TTGAAATGCT CGAGTGCCTG GGAATGCCCT GGGTCCAGGC CGCCGGGGAA GCTGAGGCCA 
     1441 TGTGTGCTTA CCTTAATGCC AGTGGCCATG TGGATGGCTG CCTCACCAAC GACGGCGATG 
     1501 CTTTCCTTTA TGGAGCCCAG ACTGTTTACA GAAATTTCAC GATGAACACA AAGGATCCCC 
     1561 ATGTTGACTG TTACACGATA TCATCTATCA AGAGTAAGCT AGGCTTGGAT CGAGACGCTC 
     1621 TGGTTGGACT GGCCGTACTT CTGGGTTGTG ATTACCTTCC AAAGGGAGTG CCTGGAGTTG 
     1681 GAAAGGAACA AGCATTAAAA CTTTTGCAGA TTTTCAAAGG TCAAAGTTTG CTTCAGAGGT 
     1741 TTAACCAGTG GATTGAAGAC CCTTGCTACT CTGTTCCACA ATCAGCACCA AAAAAAGTGG 
     1801 TTCACTGCTC TGTGTGTTCC CACCCAGGTT CGCCAAAGGA TCATGAACGT AATGGATGCA 
     1861 TATTATGTAA AAGTGATAAA TACTGTGAAC CACATGATTA TGATTACCTC TGCCCTTGTG 
     1921 AATGGCATCA GACAGACCAT AATAGGCATC TAAGTGAAAT AGAAAACAAT ATTAAAAAGA 
     1981 AAGCTTGCAG TTGCGAAGGA TTTCCATTCC ATGAGGTCAT CCAAGAGTTC CTTTTGAATA 
     2041 AGAATAAAAT GTTGAAACCG ATCACATACC AAAGACCTGA TTTATTATTG TTTCAGAGAT 
     2101 TTACTGTTCA AAAAATGGAG TGGCCCAGTC ACTATGCATG TGAAAAATTG TTGGTGCTTT 
     2161 TGACCCGCTA TGACATGATA GAAAGAAAAC ATGGTAGAAA GACCTCCAAT CAACTACAGC 
     2221 CAATTAGAAT TGTTAAACCC CGAGTCAGAA ATGGAGTCCA TTGTCTTGAA ATAGAATGGG 
     2281 AAAAGCCTGA ACATTATGTG GTGGAAGATG GAGACCCTGG AAAACTGAGC CTCCTTACAA 
     2341 TGGAAGAAGC GTCCCTGTTT GAAGCAGCCT ATCCTGATGC TGTGGCTGTC TACCAGAAAC 
     2401 AGCTGTCAGA GACCAAAGGA AGGAAACAGA AAAGTATGAA AAATAAGCCT AAAGGAAGCC 
     2461 ATTTACCGGA AGCAGATGAT GTGATCAATT CTCAGTCACT TATGACTTTA AAACCTACAT 
     2521 CTAAAGCCTT CCCCAAGCAA AATCCCAAAA TTAATTTGGA AAATTCTCCA GATCCTATTT 
     2581 TAGCACAGGA ATCTACTTCC CCCTCATTGA ATAGTTTTGT TTCCCCTGAA AATGCTCCCT 
     2641 GTTTGAATTT ACAAGAACAG TTAGTGCCAT CTCCCAGAAC TTTGGCTATA AAGCAAAGTA 
     2701 AAGATGTCAG TCATTTTCTA GTTTCAGAAT GTAGTCAGCC CAGCTCTTCC TCCCATGACA 
     2761 TCTCTGTGAT TACTGATCTG CAGTTGAGTA CCATTGATTG GGCGGGTACT TCTTTTAGTA 
     2821 ATTCTCCAGC AGTTCAAAGA AACACTTTCT CTCAAGACTT AGCATCAGAA TCTGAGTCAT 
     2881 CGGCCATCCT TCCTGACTTT GAACAGCTGT CATATGAATC AGAACAAGGG ACCTCAGACA 
     2941 GTGAAGGGTC CGGTAGAGAC CTTCAGCAGA GCAACCCTGA AGAGCAGCTC CTTTCTGGCA 
     3001 TCAGTGCTTT ACATCTTCAT GATTTGCCTC TAAAAGAAAG AATACGTATC AAATCTTCAT 
     3061 GCCCTCAGTA TAATGTAGGA GCAGATGCTG GCCTGGAAAG TTTGCCCCTA AAATTGAAAG 
     3121 GCTCTTGTAT TGCTTACAGT AGCTCTGATG GCTCATCCAA TTTTTCAAAG GATCTTACAG 
     3181 GGGTATATCT TCACAAAGAG TCCAGAAACT CTAAAGTTCT AGACAGCCGA CTCCAAGAAA 
     3241 ACTGCGGGGC GAATACTTCC TTACCTTATT CTTTCAGTGA CAAAGCAGTA AAGACTTCCA 
     3301 GTCTTCAAGT TGGGCTGCCA ACTGCTGCTA TACCTCATAA TCCAAGAGTT GCTGTGAAAA 
     3361 CTACTAAGAA CCTTGTCATG AAGAATAGTG TTTGCCTCGA GAGAGATTCC TCAGATGAAG 
     3421 ACAATGCTCC AGGGTCCTGG AAATCAAAGT ATACTGCTCC AGAAATGAAA CACAGTTCTC 
     3481 AGAAGCACAG CCTAGTCCAT GTCAGAGACA GTACTCACAA CAAACTCAGA AATCCTAAAG 
     3541 TGGAATCTAA AGAAACCAAA CTGTGTAATG AGTCTTTTAA AACAGCTGAA GATGAAGAAA 
     3601 ACGGGTTCTC AGATCTAGGG AGAAGTCCTC AGAGTTTTCG ACCATGTCAT GACAAAGATG 
     3661 AGAACTCTAC TGCCTCCTGG GAAAATCCTC TACCTTTACG CCAGAGGTTA AAACTCAGGT 
     3721 TCCAGAACAC TCAAAGTGGA TTTTATAATA CTACGCGTAC GCGGCCGCTC GAGATGGAGA 
     3781 GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG 
     3841 GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA 
     3901 ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA 
     3961 TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC 
     4021 ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG 
     4081 TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG 
     4141 TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA 
     4201 ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC 
     4261 GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT 
     4321 TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC 
     4381 GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA 
     4441 AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4501 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4561 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4621 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4681 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4741 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4801 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4861 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4921 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4981 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5041 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5101 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG 
     5161 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5221 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5281 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 
     5341 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
     5401 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 
     5461 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 
     5521 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 
     5581 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 
     5641 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 
     5701 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 
     5761 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 
     5821 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 
     5881 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 
     5941 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 
     6001 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 
     6061 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 
     6121 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 
     6181 TTCATCGACT GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC 
     6241 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 
     6301 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 
     6361 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 
     6421 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 
     6481 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 
     6541 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 
     6601 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 
     6661 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 
     6721 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 
     6781 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 
     6841 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 
     6901 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
     6961 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     7021 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     7081 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     7141 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     7201 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     7261 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     7321 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     7381 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     7441 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     7501 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     7561 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     7621 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     7681 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     7741 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     7801 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     7861 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     7921 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     7981 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     8041 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     8101 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     8161 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     8221 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     8281 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     8341 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     8401 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     8461 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     8521 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     8581 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     8641 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     8701 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     8761 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     8821 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 
     8881 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 
     8941 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 
     9001 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 
     9061 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 
     9121 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 
     9181 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 
     9241 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//