LOCUS       MG218540                9434 bp    DNA     circular  SYN 
DEFINITION  Dis3 (tGFP-tagged) - Mouse DIS3 mitotic control homolog (S. cerevisiae) (Dis3), (10ug)
ACCESSION   NM_028315
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3915..4619 
                     /label="GFP"  
     misc_feature    4666..5251 
                     /label="PolyA signal"  
     misc_feature    7343..7988 
                     /label="ColE1"  
     misc_feature    5705..6499 
                     /label="Neo"  
     misc_feature    5336..5638 
                     /label="SV40 ori"  
     misc_feature    8003..8863 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3894..3900 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3894 
                     /label="Dis3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTCAGGTCC AAGACGTTCT TGAAGAAGAC CCGCGCGGGC GGCGTGGTGA 
     1081 AGATCGTGCG CGAGCACTAC CTGCGGGATG ACATCGGCTG CGGCGCGCCG GCTTGCTCGG 
     1141 CCTGCGGCGG GGCGCACGCG GGCCCGGCCC TGGAGCTGCA GCCCCGGGAC CAGGCGAGCA 
     1201 GCCTCTGCCC GTGGCCGCAC TACCTTCTGC CGGACACCAA TGTGCTGCTG CACCAGATTG 
     1261 ATGTCCTCGA ACACCCGGCC ATCAGAAATG TCATTGTGCT ACAAACAGTG ATGCAAGAAG 
     1321 TGAGAAACCG GAGCGCCCCC ATCTACAAGC GAATCAGGGA TGTGACCAAT AACCAGGAAA 
     1381 AGCATTTCTA TACCTTCACT AATGAGCACC ATAAAGAAAC CTACATCGAG CAAGAGCAGG 
     1441 GAGAGAATGC CAATGACAGG AATGACAGAG CCATCCGAGT CGCAGCGAAG TGGTACAACG 
     1501 AGCACCTGAA GAGGGTGGCA GCAGACAGTC AGCTGCAAGT TATCCTGATA ACCAATGACA 
     1561 GGAAGAACAA AGAGAAAGCT GTGCAAGAGG GGATACCAGC CTTCACGTGT GAAGAATACG 
     1621 TAAAGAGCCT GACTGCTAAC CCTGAACTTA TAGACCGTCT TGCTTACTTG TCCGATGAAA 
     1681 TGAATGAAAT AGAAAGTGGG AAAATAATAT TTTCAGAGCA TCTTCCCTTA AGCAAGCTCC 
     1741 AACAAGGCAT AAAATCTGGT TCCTATCTTC AAGGAACATT CAGAGCTAGC AGGGAAAATT 
     1801 ATTTGGAGGC TACAGTATGG ATTCATGGAG ACAAAGAAGA GGAAAAAGAG ATACTTATAC 
     1861 AGGGAATTAA GCATCTAAAC AGAGCTGTGC ATGAAGACAT TGTGGCCGTG GAGCTACTGC 
     1921 CCAGGAGCCA GTGGGTGGCA CCGTCTTCCG TGGTTTTAGA CGATGAAGGT CAAAATGAAG 
     1981 ACGATGTGGA GAAAGATGAG GAGAGAGAAC TCCTGCTTAA GACTGCTGTA AGTGAAAAAA 
     2041 TGTTACGGCC TACAGGTCGA GTTGTGGGGA TAATAAAAAG GAACTGGAGA CCGTATTGTG 
     2101 GCATGCTTTC CAAGTCTGAT ATTAAGGAGT CAAGAAGACA TCTCTTTACA CCCGCTGATA 
     2161 AGAGAATTCC ACGAATTCGG ATAGAAACCA GACAGGCTTC TGCGTTAGAA GGACGGAGAA 
     2221 TTATTGTCGC TATTGATGGT TGGCCTAGAA ATTCTAGATA TCCAAATGGA CACTTTGTAA 
     2281 AGAATTTAGG CGATGTTGGA GAGAAGGAGA CAGAAACGGA AGTGTTGCTG CTCGAGCACG 
     2341 ATGTTCCTCA TCAGCCCTTT TCCCAGGCTG TGCTTAGCTT CCTGCCCAGG ATGCCCTGGA 
     2401 GCATTACTGA GGAGGACATG AAAAACCGAG AAGACCTGAG ACATCTGTGT GTTTGCAGTG 
     2461 TGGACCCTCC AGGGTGCACT GACATAGATG ACGCTCTGCA TTGTAGAGAG CTCAGCAATG 
     2521 GAAACTTGGA GGTTGGTGTT CATATTGCGG ATGTTAGCCA TTTTATCAGG CCAGGAAATG 
     2581 CGTTGGATCA AGAATCTGCA AGAAGAGGAA CAACTGTTTA TCTTTGTGAA AAGAGGATTG 
     2641 ACATGGTTCC AGAGTTGCTC AGCTCCAACC TCTGTTCCTT AAGATCCAAC GTTGACAGGT 
     2701 TGGCATTTTC CTGTATTTGG GAAATGAATC ATAATGCTGA AATATTAAAA ACGAGATTTA 
     2761 CCAAAAGTGT CATTAATTCA AAGGCTTCTC TTACGTACGC GGAAGCACAG ATGAGAATTG 
     2821 ATTCGGCGGC TATGAATGAT GATATTACCA CTAGTCTCCG TGGACTCAAT CAGCTGGCTA 
     2881 AAATTCTAAA AAAGGGAAGG ATTGAAAAGG GGGCTTTGAC TCTGTCTTCT CCAGAGATCC 
     2941 GATTCCACAT GGACAGTGAA ACCCACGACC CAATCGACCT GCAGACGAAA GAGCTGAGAG 
     3001 AAACAAATTC CATGGTGGAA GAATTTATGT TACTTGCTAA TATTTCTGTC GCAAAAAAAA 
     3061 TTCATGAAGA GTTTTCTGAA CATGCTCTGC TTCGGAAACA CCCAGCTCCG CCTCCCTCGA 
     3121 ATTATGACAT TCTTGTTAAG GCTGCAAAGT CCAAGAATTT GCAAATTAAG ACTGATACAG 
     3181 CCAAATCTTT GGCCGACTCT TTGGACCGGG CTGAATCTCC GGATTTCCCA TACCTGAATA 
     3241 CTCTATTAAG GATACTGGCC ACTCGCTGTA TGATGCAAGC TGTGTACTTC TGCTCTGGGA 
     3301 TGGATAATGA TTTTCATCAC TATGGCTTAG CCTCCCCCAT ATACACACAT TTCACCTCTC 
     3361 CTATCAGAAG ATATGCAGAC ATAATTGTCC ATCGGCTATT AGCTGTGGCG ATTGGGGCTG 
     3421 ACTGTACTTA CCCTGAGTTG ACAGACAAAC ACAAGCTTTC AGATATATGT AAAAACCTCA 
     3481 ATTTCCGGCA TAAAATGGCT CAGTATGCCC AGCGTGCTTC GGTGGCTTTT CATACACAGT 
     3541 TGTTTTTCAA AAGCAAAGGA ATAGTAAGTG AAGAAGCCTA TATTCTCTTT GTAAGGAAAA 
     3601 ATGCAATTGT GGTGTTAATT CCAAAGTATG GCTTAGAAGG TACAGTTTTC TTTGAAGAAA 
     3661 AAGATAAACC AAAGCCACGC CTTGCTTACG ATGATGAGAT CCCTTCCCTG AGAATAGAAG 
     3721 GTACAGTGTT CCATGTGTTT GATAAGGTTA AAGTGAAGAT CACATTAGAT TCATCAAATC 
     3781 TTCAACATCA GAAAATCCGC ATGGCCCTTG TAGAACCACA GATCCCAGGA ATAAATATTC 
     3841 CTCCTAATGT TGCAGACAAG GCTCTTACTG CACCAGGGGG AAAGAAGAGG AAGCTTGAGA 
     3901 AGACGCGTAC GCGGCCGCTC GAGATGGAGA GCGACGAGAG CGGCCTGCCC GCCATGGAGA 
     3961 TCGAGTGCCG CATCACCGGC ACCCTGAACG GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG 
     4021 AGGGCACCCC CGAGCAGGGC CGCATGACCA ACAAGATGAA GAGCACCAAA GGCGCCCTGA 
     4081 CCTTCAGCCC CTACCTGCTG AGCCACGTGA TGGGCTACGG CTTCTACCAC TTCGGCACCT 
     4141 ACCCCAGCGG CTACGAGAAC CCCTTCCTGC ACGCCATCAA CAACGGCGGC TACACCAACA 
     4201 CCCGCATCGA GAAGTACGAG GACGGCGGCG TGCTGCACGT GAGCTTCAGC TACCGCTACG 
     4261 AGGCCGGCCG CGTGATCGGC GACTTCAAGG TGATGGGCAC CGGCTTCCCC GAGGACAGCG 
     4321 TGATCTTCAC CGACAAGATC ATCCGCAGCA ACGCCACCGT GGAGCACCTG CACCCCATGG 
     4381 GCGATAACGA TCTGGATGGC AGCTTCACCC GCACCTTCAG CCTGCGCGAC GGCGGCTACT 
     4441 ACAGCTCCGT GGTGGACAGC CACATGCACT TCAAGAGCGC CATCCACCCC AGCATCCTGC 
     4501 AGAACGGGGG CCCCATGTTC GCCTTCCGCC GCGTGGAGGA GGATCACAGC AACACCGAGC 
     4561 TGGGCATCGT GGAGTACCAG CACGCCTTCA AGACCCCGGA TGCAGATGCC GGTGAAGAAA 
     4621 GAGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4681 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4741 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4801 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4861 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4921 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4981 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5041 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5101 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5161 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5221 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5281 CCTGTTGAAT TGGGTACGCG GCCAGCTTGG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA 
     5341 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5401 ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     5461 AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC 
     5521 AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG 
     5581 GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC 
     5641 TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA 
     5701 TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG 
     5761 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC 
     5821 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT 
     5881 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT 
     5941 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC 
     6001 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC 
     6061 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC 
     6121 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG 
     6181 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG 
     6241 GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT 
     6301 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCC 
     6361 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA 
     6421 TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC 
     6481 TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC 
     6541 AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT 
     6601 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA 
     6661 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA 
     6721 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT 
     6781 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT 
     6841 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC 
     6901 ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC 
     6961 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC 
     7021 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG 
     7081 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 
     7141 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 
     7201 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 
     7261 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 
     7321 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 
     7381 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 
     7441 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 
     7501 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 
     7561 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 
     7621 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 
     7681 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 
     7741 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTTTTTTTG 
     7801 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 
     7861 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 
     7921 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT 
     7981 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA 
     8041 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA 
     8101 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC 
     8161 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA 
     8221 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG 
     8281 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG 
     8341 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG 
     8401 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG 
     8461 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC 
     8521 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT 
     8581 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA 
     8641 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA 
     8701 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA 
     8761 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC 
     8821 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC 
     8881 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 
     8941 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 
     9001 CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA 
     9061 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG 
     9121 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT 
     9181 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG 
     9241 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA 
     9301 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT 
     9361 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT 
     9421 TTAACGCGAA TTTT
//