LOCUS       MG218180                9575 bp    DNA     circular  SYN 
DEFINITION  Rbm27 (tGFP-tagged) - Mouse RNA binding motif protein 27 (Rbm27), (10ug)
ACCESSION   NM_172626
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4056..4760 
                     /label="GFP"  
     misc_feature    4807..5392 
                     /label="PolyA signal"  
     misc_feature    7484..8129 
                     /label="ColE1"  
     misc_feature    5846..6640 
                     /label="Neo"  
     misc_feature    5477..5779 
                     /label="SV40 ori"  
     misc_feature    8144..9004 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4035..4041 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4035 
                     /label="Rbm27"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTCATAGAA GATGTGGATG CCCTCAAGTC CTGGCTGGCC AAGTTACTGG 
     1081 AGCCGATATG TGATGCTGAC CCTTCAGCCC TAGCCAACTA TGTTGTAGCA CTGGTCAAGA 
     1141 AGGACAAACC TGAGAAGGAA TTGAAAGCCT TTTGTGCTGA CCAACTTGAT GTCTTTTTAC 
     1201 AAAAAGAGAC TTCAGGTTTT GTGGACAAAC TATTTGAAAG TCTTTATACT AAGAACTACC 
     1261 TTCCACCATT GGAACCAGTT AAACCTGAGC CAAAACCACT AGTCCAAGAA AAAGAAGAAA 
     1321 TTAAAGAAGA GGTATTTCAA GAGCCAGCAG AAGAAGAACG AGATACTAGA AAGAAGAAAT 
     1381 ATCCTAGTCC TCAGAAGAGT CGTTCAGAAT CTAGTGAACG AAGAACACGT GAGAAGAAAA 
     1441 GAGAAGATGG CAAGTGGAGA GACTATGAGC GGTACTACGA GCGGAATGAG CTGTATCGGG 
     1501 AGAAGTATGA CTGGAGAAGG GGCAGGAGCA AGAGCAGGAG TAAGAGCCGA GGCTTGAGTC 
     1561 GGAGTAGAAG CCGGAGCAGG GGCCGCAGCA AAGACCGGGA TCCAAACAGG AACGTTGAGC 
     1621 ACAGGGAAAG ATCAAAGTTT AAGAGCGAAA GAAATGACCT GGAGAGCTCT TATGTGCCTG 
     1681 TGTCTGCTCC ACCTCCAAGC TCTTCTGAGC AGTATTCCTC TGGGGCACAG TCTATTCCCA 
     1741 GCACTGTCAC TGTGATTGCA CCTGCGCACC ATTCTGAGAA CACAACTGAG AGCTGGTCTA 
     1801 ATTACTATAA CAATCATAGC TCTTCCAATT CTTTTGGTCG AAACCCACCA CCAAAGAGGC 
     1861 GCTGCAGAGA TTACGATGAA AGAGGATTTT GTGTACTTGG TGACCTTTGT CAGTTTGATC 
     1921 ATGGAAATGA TCCTCTAGTT GTTGATGAAG TTGCTCTGCC AAGTATGATT CCTTTCCCAC 
     1981 CCCCTCCTCC TGGGCTTCCT CCTCCACCAC CTCCCGGAAT GTTAATGCCT CCAATGCCAG 
     2041 GTCCAGGCCC AGGCCCTGGT CCTGGCCCTG GCCCTGGTCC CGGCCCAGGC CCAGGCCCTG 
     2101 GTCATAGTAT GAGACTTCCT GTTCCTCAAG GACATGGTCA GCCTCCACCT TCTGTTGTGC 
     2161 TTCCCATACC AAGACCTCCC ATTTCACAGT CAAGTTTAAT AAACAGCCGT GACCAGCCTG 
     2221 GGACAAGTGC AGTGCCCAAT CTTGCACCAG TGGGAGCAAG ACTACCTCCT CCTTTACCGC 
     2281 AGAACCTCCT TTATACAGTA TCAGAAAATA CATATGAACC AGATGGCTAT AATCCTGAAG 
     2341 CACCTAGCAT CACCAGTTCT GGTAGATCAC AGTACAGACA GTTCTTCTCA AGAGCACAGA 
     2401 CACAGCGTCC CAACCTGATT GGCCTCACAT CTGGAGATAT GGATGCAAAT CCAAGAGCTG 
     2461 CTAATATTGT CATCCAGACT GAACCACCAG TTCCTGTTTC AGTTAATAGC AACGTCACCA 
     2521 GAGTAGTCCT GGAACCAGAG AGCCGAAAGA GAGCTATTAG TGGTTTGGAG GGGCCACTTA 
     2581 CAAAGAAGCC TTGGCTGGGA AAACAAGGGA ACAACAATCA AAGTAAACCA GGCTTCCTGA 
     2641 GGAAGAATCA TTATACAAAC ACCAAACTAG AGGTGAAAAA AATCCCCCAG GAATTGAACA 
     2701 ACATTACCAA GCTCAACGAA CACTTCAGCA AATTTGGAAC CATAGTTAAT ATCCAGGTTG 
     2761 CTTTTAAGGG TGATCCAGAA GCAGCACTCA TCCAATACCT TACCAATGAA GAGGCCAGGA 
     2821 AAGCCATTTC TAGCACAGAA GCAGTTTTGA ACAATCGGTT CATTCGAGTC CTGTGGCATA 
     2881 GAGAGAACAA TGAGCAACCA GCACTCCAGT CCTCAGCACA GATTCTCCTG CAGCAGCAAC 
     2941 ACACCCTGAG TCACCTTTCA CAGCAACACC ATAGCCTCCC ACAGCATCTT CATCCGCAGC 
     3001 AGGTGATGGT AACCCAGTCT TCCCCCTCAT CGGTCCATGG AGGTATCCAA AAGATGATGG 
     3061 GCAAACCACA GACCTCTGGT GCCTATGTTC TTAACAAAGT CCCTGTTAAA CACCGTCTTG 
     3121 GACATGCAAG TACTAACCAG AGTGACACGT CTCACTTGCT AAATCAGACT GGTGGTTCTT 
     3181 CTGGGGAAGA TTGTCCGGTA TTTTCTACTC CAGGCCATCC AAAAACAATT TACAGCTCTT 
     3241 CAAACTTAAA GGCACCTTCA AAACTTTGTT CAGGGTCTAA ATCTCATGAT GTTCAAGAAG 
     3301 TTCTTAAGAA GAAACAGGAA GCAATGAAGT TACAGCAAGA CATGAGGAAA AAGAAACAAG 
     3361 AAATGTTGGA AAAGCAGATA GAATGCCAGA AGATGCTGAT TTCTAAACTA GAAAAGAACA 
     3421 AAAACATGAA ACCAGAAGAA AGAGCAAATA TAATGAAGAC CTTGAAAGAA CTGGGAGAGA 
     3481 AGATCTCACA ATTGAAAGAT GAATTAAAAA CATCTTCTAC AGTCTCCACA CCGTCCAAAG 
     3541 TAAAGACAAA AACAGAGGCC CAGAAGGAGT TGTTGGACAC AGAGTTGGAC CTCCACAAGA 
     3601 GGCTCTCTTC AGGAGAAGAT ACAACAGAAT TACGTAAAAA GCTCAGTCAG TTACAAGTTG 
     3661 AGGCTGCAAG ATTAGGTATT TTACCTGTGG GGAGAGGAAA GACCATATCC TCTCAAGGCC 
     3721 GAGGAAGAGG CAGGGGTCGA GGAAGAGGGA GGGGCTCACT GAATCACATG GTGGTGGATC 
     3781 ACCGCCCCAA AGCTCTCCCA GGTGGAGGAT TCATTGAGGA GGAAAAAGAT GAGTTGCTTC 
     3841 AGCATTTCTC AGCTACAAAC CAAGCATCAA AATTCAAAGA CCGTCGGCTA CAGATCTCAT 
     3901 GGCATAAGCC CAAGGTACCA TCTATATCTA CTGAGACCGA GGAAGAGGAA GTCAAAGAAG 
     3961 AGGAAACAGA AACCTCAGAT TTGTTTTTGC ATGATGATGA TGATGAAGAT GAAGATGAAT 
     4021 ATGAGTCTCG TTCGTGGCGA AGAACGCGTA CGCGGCCGCT CGAGATGGAG AGCGACGAGA 
     4081 GCGGCCTGCC CGCCATGGAG ATCGAGTGCC GCATCACCGG CACCCTGAAC GGCGTGGAGT 
     4141 TCGAGCTGGT GGGCGGCGGA GAGGGCACCC CCGAGCAGGG CCGCATGACC AACAAGATGA 
     4201 AGAGCACCAA AGGCGCCCTG ACCTTCAGCC CCTACCTGCT GAGCCACGTG ATGGGCTACG 
     4261 GCTTCTACCA CTTCGGCACC TACCCCAGCG GCTACGAGAA CCCCTTCCTG CACGCCATCA 
     4321 ACAACGGCGG CTACACCAAC ACCCGCATCG AGAAGTACGA GGACGGCGGC GTGCTGCACG 
     4381 TGAGCTTCAG CTACCGCTAC GAGGCCGGCC GCGTGATCGG CGACTTCAAG GTGATGGGCA 
     4441 CCGGCTTCCC CGAGGACAGC GTGATCTTCA CCGACAAGAT CATCCGCAGC AACGCCACCG 
     4501 TGGAGCACCT GCACCCCATG GGCGATAACG ATCTGGATGG CAGCTTCACC CGCACCTTCA 
     4561 GCCTGCGCGA CGGCGGCTAC TACAGCTCCG TGGTGGACAG CCACATGCAC TTCAAGAGCG 
     4621 CCATCCACCC CAGCATCCTG CAGAACGGGG GCCCCATGTT CGCCTTCCGC CGCGTGGAGG 
     4681 AGGATCACAG CAACACCGAG CTGGGCATCG TGGAGTACCA GCACGCCTTC AAGACCCCGG 
     4741 ATGCAGATGC CGGTGAAGAA AGAGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4801 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4861 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4921 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4981 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5041 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5101 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5161 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5221 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5281 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5341 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5401 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCTTG GCTGTGGAAT 
     5461 GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     5521 ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     5581 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC 
     5641 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT 
     5701 TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA 
     5761 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC 
     5821 GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC 
     5881 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA 
     5941 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT 
     6001 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG 
     6061 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA 
     6121 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 
     6181 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG 
     6241 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG 
     6301 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC 
     6361 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT 
     6421 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC 
     6481 TGTGGCCGGC TGGGTGTGGC CGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT 
     6541 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT 
     6601 CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC 
     6661 TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA 
     6721 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA 
     6781 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG 
     6841 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT 
     6901 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC 
     6961 CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT 
     7021 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG 
     7081 GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT 
     7141 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT 
     7201 TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC 
     7261 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG 
     7321 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG 
     7381 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 
     7441 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG 
     7501 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 
     7561 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG 
     7621 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT 
     7681 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 
     7741 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT 
     7801 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC 
     7861 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA 
     7921 CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 
     7981 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 
     8041 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 
     8101 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 
     8161 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 
     8221 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 
     8281 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 
     8341 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 
     8401 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 
     8461 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 
     8521 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 
     8581 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 
     8641 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 
     8701 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 
     8761 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 
     8821 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 
     8881 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 
     8941 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 
     9001 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 
     9061 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 
     9121 CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 
     9181 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 
     9241 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 
     9301 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 
     9361 AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 
     9421 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 
     9481 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 
     9541 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//