LOCUS       MG217895                7814 bp    DNA     circular  SYN 
DEFINITION  Actr3 (tGFP-tagged) - Mouse ARP3 actin-related protein 3 homolog (yeast) (Actr3), (10ug)
ACCESSION   NM_023735
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2295..2999 
                     /label="GFP"  
     misc_feature    3046..3631 
                     /label="PolyA signal"  
     misc_feature    5723..6368 
                     /label="ColE1"  
     misc_feature    4085..4879 
                     /label="Neo"  
     misc_feature    3716..4018 
                     /label="SV40 ori"  
     misc_feature    6383..7243 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2274..2280 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2274 
                     /label="Actr3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGGACGG CTGCCGGCCT GTGTGGTGGA CTGTGGCACG GGATATACAA 
     1081 AACTAGGATA TGCTGGAAAT ACAGAGCCAC AGTTTATCAT CCCATCATGT ATTGCCATTA 
     1141 AAGAGTCTGC AAAAGTGGGT GACCAAGCCC AGAGGAGGGT GATGAAAGGC GTGGATGACC 
     1201 TAGACTTCTT CATTGGTGAT GAAGCAATAG AAAAGCCCAC ATATGCAACA AAGTGGCCAA 
     1261 TTCGCCATGG TATAGTTGAA GACTGGGACT TAATGGAAAG GTTTATGGAG CAAGTGATTT 
     1321 TTAAATATTT AAGGGCAGAA CCTGAAGATC ATTACTTTCT TTTGACTGAA CCTCCACTGA 
     1381 ATACTCCAGA AAACAGGGAA TATACTGCTG AAATAATGTT TGAATCCTTC AATGTTCCAG 
     1441 GCTTGTACAT TGCTGTGCAG GCTGTTCTTG CCTTAGCTGC ATCCTGGACC TCAAGACAAG 
     1501 TAGGAGAGCG GACGCTGACG GGTACAGTAA TAGACAGTGG AGACGGAGTC ACTCATGTCA 
     1561 TTCCTGTGGC TGAAGGATAT GTTATCGGCA GCTGTATTAA ACACATTCCA ATCGCAGGAA 
     1621 GAGATATAAC ATATTTTATT CAGCAACTGC TGCGAGACCG AGAAGTAGGA ATCCCTCCTG 
     1681 AGCAGTCCTT GGAAACTGCG AAAGCAGTGA AGGAACGCTA CAGTTATGTC TGCCCAGATT 
     1741 TAGTAAAAGA GTTTAACAAG TATGACACCG ATGGGTCAAA GTGGATCAAA CAGTACACCG 
     1801 GAGTCAACGC CATCTCAAAG AAAGAGTTTT CTATTGATGT TGGCTATGAG CGATTCCTGG 
     1861 GACCCGAGAT CTTTTTCCAT CCAGAGTTTG CTAATCCAGA TTTTACACAA CCTATCTCAG 
     1921 AAGTTGTAGA TGAAGTCATT CAGAATTGCC CCATTGATGT CCGGCGTCCT CTCTACAAGA 
     1981 ACATTGTCCT CTCTGGTGGT TCAACCATGT TCAGGGACTT TGGACGTCGT TTGCAAAGAG 
     2041 ATTTGAAGAG AACTGTAGAT GCCAGGCTGA AGTTAAGCGA GGAGCTGAGT GGTGGTAGAT 
     2101 TGAAGCCCAA GCCTATTGAT GTACAAGTTA TTACACACCA TATGCAGCGG TATGCAGTCT 
     2161 GGTTTGGAGG GTCAATGCTG GCTTCCACGC CTGAGTTCTA CCAAGTATGC CACACCAAAA 
     2221 AGGATTATGA AGAAATTGGA CCTAGCATTT GTCGTCACAA TCCAGTGTTT GGAGTCATGT 
     2281 CCACGCGTAC GCGGCCGCTC GAGATGGAGA GCGACGAGAG CGGCCTGCCC GCCATGGAGA 
     2341 TCGAGTGCCG CATCACCGGC ACCCTGAACG GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG 
     2401 AGGGCACCCC CGAGCAGGGC CGCATGACCA ACAAGATGAA GAGCACCAAA GGCGCCCTGA 
     2461 CCTTCAGCCC CTACCTGCTG AGCCACGTGA TGGGCTACGG CTTCTACCAC TTCGGCACCT 
     2521 ACCCCAGCGG CTACGAGAAC CCCTTCCTGC ACGCCATCAA CAACGGCGGC TACACCAACA 
     2581 CCCGCATCGA GAAGTACGAG GACGGCGGCG TGCTGCACGT GAGCTTCAGC TACCGCTACG 
     2641 AGGCCGGCCG CGTGATCGGC GACTTCAAGG TGATGGGCAC CGGCTTCCCC GAGGACAGCG 
     2701 TGATCTTCAC CGACAAGATC ATCCGCAGCA ACGCCACCGT GGAGCACCTG CACCCCATGG 
     2761 GCGATAACGA TCTGGATGGC AGCTTCACCC GCACCTTCAG CCTGCGCGAC GGCGGCTACT 
     2821 ACAGCTCCGT GGTGGACAGC CACATGCACT TCAAGAGCGC CATCCACCCC AGCATCCTGC 
     2881 AGAACGGGGG CCCCATGTTC GCCTTCCGCC GCGTGGAGGA GGATCACAGC AACACCGAGC 
     2941 TGGGCATCGT GGAGTACCAG CACGCCTTCA AGACCCCGGA TGCAGATGCC GGTGAAGAAA 
     3001 GAGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3061 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3121 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3181 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     3241 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     3301 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     3361 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     3421 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     3481 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     3541 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     3601 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     3661 CCTGTTGAAT TGGGTACGCG GCCAGCTTGG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA 
     3721 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     3781 ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     3841 AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC 
     3901 AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG 
     3961 GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC 
     4021 TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA 
     4081 TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG 
     4141 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC 
     4201 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT 
     4261 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT 
     4321 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC 
     4381 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC 
     4441 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC 
     4501 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG 
     4561 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG 
     4621 GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT 
     4681 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCC 
     4741 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA 
     4801 TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC 
     4861 TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC 
     4921 AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT 
     4981 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA 
     5041 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA 
     5101 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT 
     5161 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT 
     5221 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC 
     5281 ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC 
     5341 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC 
     5401 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG 
     5461 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 
     5521 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 
     5581 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 
     5641 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 
     5701 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 
     5761 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 
     5821 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 
     5881 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 
     5941 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 
     6001 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 
     6061 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 
     6121 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTTTTTTTG 
     6181 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 
     6241 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 
     6301 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT 
     6361 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA 
     6421 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA 
     6481 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC 
     6541 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA 
     6601 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG 
     6661 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG 
     6721 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG 
     6781 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG 
     6841 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC 
     6901 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT 
     6961 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA 
     7021 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA 
     7081 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA 
     7141 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC 
     7201 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC 
     7261 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 
     7321 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 
     7381 CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA 
     7441 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG 
     7501 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT 
     7561 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG 
     7621 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA 
     7681 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT 
     7741 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT 
     7801 TTAACGCGAA TTTT
//