LOCUS       MG217704                9200 bp    DNA     circular  SYN 
DEFINITION  Akap2 (tGFP-tagged) - Mouse A kinase (PRKA) anchor protein 2 (Akap2) transcript variant 2, (10ug)
ACCESSION   NM_001035532
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3681..4385 
                     /label="GFP"  
     misc_feature    4432..5017 
                     /label="PolyA signal"  
     misc_feature    7109..7754 
                     /label="ColE1"  
     misc_feature    5471..6265 
                     /label="Neo"  
     misc_feature    5102..5404 
                     /label="SV40 ori"  
     misc_feature    7769..8629 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3660..3666 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3660 
                     /label="MG217704"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAATTGGG GTGTCCGTGG CAGAATGTAA AAGTGTACCT GGAGTCACCT 
     1081 CTACCCCCCA TTCCAAGGAC CACTCCTCCC CTTTCTATTC GCCCTCACAC AATGGCCTCC 
     1141 TCGCTGATCA CCACGAGTCT TTAGACAACG ATGTCGCCAG AGAGATCCAG TACCTAGATG 
     1201 AAGTGCTGGA GGCCAACTGC TGTGATTCCT CTGTGGATGG AACTTACAAC GGAATCTCCT 
     1261 CCCCAGAGCC TGGTGCAGCC ATACTGGTAA GCAGCTTAGG CTCGCCTGCC CACTCAGTCA 
     1321 CCGAGGCAGA ACCCACTGAG AAAGCATCCG GTAGGCAGGT GCCTCCTCAC ATCGAGCTCA 
     1381 GTAGAAGCCC CTCCGACAGA ATGGCTGAAG GAGAAAGAGC CAATGGACAC TCCACTGACC 
     1441 AGCCCCAAGA CCTGCTGGGG AACAGCCTGC AGGCACCTGC CAGCCCCAGT TCCTCTACCA 
     1501 GCTCCCACTG CTCCTCCCGA GATGGGGAGT TCACACTCAC CACACTGAAG AAGGAGGCCA 
     1561 AGTTTGAGCT GCGTGCCTTC CACGAGGACA AGAAGCCCTC CAAGCTCTTT GAGGAGGACG 
     1621 AGCGCGAGAA GGAGCAGTTC TGCGTCCGGA AAGTGAGGCC CTCCGAGGAG ATGATAGAGC 
     1681 TGGAGAAAGA GCGGAGGGAG CTCATCCGGA GTCAGGCTGT GAAGAAGAAT CCTGGCATCG 
     1741 CCGCCAAGTG GTGGAATCCT CCCCAGGAGA AAACTATTGA GGAGCAGCTG GACGAGGAAC 
     1801 ATCTGGAGTC GCACAGAAAG TACAAGGAGC GCAAAGAGAA AAGGGCCCAG CAGGAGCAGC 
     1861 TACAGTTGCA ACAGCAGCAG CAGCAACAGC TGCAACAGCA ACAGCTACAA CAACAACAGC 
     1921 TGCAACAGCA ACAGCTGCAA CAACAGCTGC AACAGCAGCA GCTGTCCACG TCCCAGCCCT 
     1981 GCACAGCCCC AGCTGCTCAC AAACACCTAG ATGGCATTGA ACACACAAAG GAGGATGTGG 
     2041 TCACTGAGCA GATTGACTTC TCGGCTGCAC GCAAGCAGTT CCAGCTGATG GAGAATTCCA 
     2101 GGCAGACACT GGCCAAGGGC CAGAGCACAC CAAGGCTGTT CTCTATCAAG CCTTACTACA 
     2161 AACCTCTTGG ATCCATCCAT TCGGACAAGC CACCGACCAT CTTGAGACCA GCTACTGTCG 
     2221 GGGGAACTCT GGAAGACGGC GGCACACAGG CAGCCAAGGA GCAGAAAGCT CCCTGTGTAT 
     2281 CTGAGAGCCA GTCTGCAGGA GCCGGTCCAG CTAACGCTGC CACTCAGGGA AAGGAAGGGC 
     2341 CATACAGTGA GCCTTCGAAA CGCGGGCCCT TATCTAAACT GTGGGCAGAG GATGGAGAGT 
     2401 TCACAAGTGC CCGGGCCGTC CTAACTGTAG TCAAAGATGA AGATCATGGG ATTTTGGACC 
     2461 AATTTTCAAG ATCGGTCAAT GTATCTTTGA CTCAGGAGGA GCTGGACTCA GGGTTAGATG 
     2521 AGTTGTCAGT GAGGTCACAG GATACCACTG TCCTGGAGAC TCTGTCCAAT GACTTCAGCA 
     2581 TGGACAACAT AAGTGACAGT GGAGCTTCTA ACGAGACACC CAGTGCCCTT CAGGAAAACT 
     2641 CGCTGGCTGA TTTCTCTCTG CCTCAGACCC CCCAAACAGA CAACCCCTCC GAGGGCCGAG 
     2701 AAGGGGTCTC TAAGTCATTC AGTGACCATG GTTTCTATTC TCCTTCCTCC ACATTGGGAG 
     2761 ATTCTCCGTC CGTTGATGAC CCCTTGGAAT ATCAGGCTGG GCTCCTGGTG CAGAATGCCA 
     2821 TTCAACAAGC CATAGCCGAG CAGGTGGATA AGGCTGAGGC GCACACCAGC AAGGAAGGAT 
     2881 CAGAACAGCA GGAGCCTGAA GCAACGGTAG AGGAAGCTGG AAGCCAGACA CCTGGCTCTG 
     2941 AGAAGCCCCA GGGCATGTTT GCACCACCTC AGGTGTCCTC TCCTGTTCAA GAGAAAAGGG 
     3001 ATATATTACC AAAGAACCTC CCTGCGGAGG ACAGGGCACT CAGGGAAAAG GGGCCCTCCC 
     3061 AGCCACCCAC GGCTGCGCAG CCCAGCGGTC CAGTCAACAT GGAAGAGACC AGGCCTGAAG 
     3121 GAGGGTACTT CAGCAAGTAT TCAGAGGCAG CTGAGCTGAG AAGCACAGCC TCCCTCCTGG 
     3181 CGACTCAAGA GTCTGATGTC ATGGTTGGGC CCTTCAAACT GAGGTCAAGG AAGCAGCGGA 
     3241 CTTTGTCCAT GATCGAAGAA GAGATCCGGG CAGCCCAGGA AAGGGAGGAG GAGCTAAAGC 
     3301 GGCAGAGACA AGTGAGGCAA AGCACCCCAA GCCCCAGGGC CAAGAACGCC CCATCGCTGC 
     3361 CTTCTAGGAC CACCTGCTAC AAGACTGCTC CAGGGAAAAT AGAGAAAGTC AAACCTCCTC 
     3421 CATCCCCCAC AACTGAAGGT CCCAGCTTAC AGCCTGACTT AGCCCCTGAA GAGGCTGCCG 
     3481 GAACCCAGCG GCCCAAGAAC CTGATGCAGA CCCTCATGGA AGACTATGAG ACCCACAAAT 
     3541 CTAAAAGGCG CGAGAGAATG GATGATAGTA GTGTCCTGGA GGCCACACGG GTTAATCGGA 
     3601 GGAAGAGTGC TCTGGCCTTG CGCTGGGAGG CAGGGATTTA CGCTAACCAG GAAGAGGAGG 
     3661 ACAACGAAAC GCGTACGCGG CCGCTCGAGA TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA 
     3721 TGGAGATCGA GTGCCGCATC ACCGGCACCC TGAACGGCGT GGAGTTCGAG CTGGTGGGCG 
     3781 GCGGAGAGGG CACCCCCGAG CAGGGCCGCA TGACCAACAA GATGAAGAGC ACCAAAGGCG 
     3841 CCCTGACCTT CAGCCCCTAC CTGCTGAGCC ACGTGATGGG CTACGGCTTC TACCACTTCG 
     3901 GCACCTACCC CAGCGGCTAC GAGAACCCCT TCCTGCACGC CATCAACAAC GGCGGCTACA 
     3961 CCAACACCCG CATCGAGAAG TACGAGGACG GCGGCGTGCT GCACGTGAGC TTCAGCTACC 
     4021 GCTACGAGGC CGGCCGCGTG ATCGGCGACT TCAAGGTGAT GGGCACCGGC TTCCCCGAGG 
     4081 ACAGCGTGAT CTTCACCGAC AAGATCATCC GCAGCAACGC CACCGTGGAG CACCTGCACC 
     4141 CCATGGGCGA TAACGATCTG GATGGCAGCT TCACCCGCAC CTTCAGCCTG CGCGACGGCG 
     4201 GCTACTACAG CTCCGTGGTG GACAGCCACA TGCACTTCAA GAGCGCCATC CACCCCAGCA 
     4261 TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT TCCGCCGCGT GGAGGAGGAT CACAGCAACA 
     4321 CCGAGCTGGG CATCGTGGAG TACCAGCACG CCTTCAAGAC CCCGGATGCA GATGCCGGTG 
     4381 AAGAAAGAGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4441 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4501 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4561 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4621 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4681 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4741 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4801 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4861 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4921 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4981 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5041 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCTTGGCTGT GGAATGTGTG TCAGTTAGGG 
     5101 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5161 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5221 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT 
     5281 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 
     5341 GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 
     5401 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG 
     5461 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 
     5521 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 
     5581 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 
     5641 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG 
     5701 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 
     5761 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 
     5821 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 
     5881 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 
     5941 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG 
     6001 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG 
     6061 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 
     6121 GTGGCCGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 
     6181 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 
     6241 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA 
     6301 CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG 
     6361 AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG 
     6421 ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 
     6481 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 
     6541 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT 
     6601 AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA 
     6661 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA 
     6721 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT 
     6781 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT 
     6841 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT 
     6901 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 
     6961 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 
     7021 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 
     7081 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 
     7141 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 
     7201 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 
     7261 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 
     7321 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 
     7381 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 
     7441 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 
     7501 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTT 
     7561 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA 
     7621 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 
     7681 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 
     7741 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG 
     7801 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT 
     7861 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG 
     7921 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 
     7981 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 
     8041 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 
     8101 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 
     8161 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 
     8221 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 
     8281 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 
     8341 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 
     8401 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 
     8461 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 
     8521 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 
     8581 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 
     8641 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 
     8701 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 
     8761 TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA 
     8821 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT 
     8881 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT 
     8941 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC 
     9001 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT 
     9061 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT 
     9121 TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC 
     9181 AAAAATTTAA CGCGAATTTT 
//