LOCUS       MG217443                9182 bp    DNA     circular  SYN 
DEFINITION  Pla2g4e (tGFP-tagged) - Mouse phospholipase A2 group IVE (Pla2g4e), (10ug)
ACCESSION   NM_177845
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3663..4367 
                     /label="GFP"  
     misc_feature    4414..4999 
                     /label="PolyA signal"  
     misc_feature    7091..7736 
                     /label="ColE1"  
     misc_feature    5453..6247 
                     /label="Neo"  
     misc_feature    5084..5386 
                     /label="SV40 ori"  
     misc_feature    7751..8611 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3642..3648 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3642 
                     /label="Pla2g4e"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGTCTATT CCACACTCCG ATGAAGCAGA CGTGGCTGGG ATGACCCACG 
     1081 CCTCAGAAGG CCACCATGGC CTAGGGACCA GCATGCTTGT CCCACAGAAC CCACAAGGGT 
     1141 CAGAAGACAG CAAGCCAGCA AGAAACTGCA GTGGATTTGA AGATGCACAG GACCCACAGA 
     1201 CTGCTGTGCC CTCACCTTTA CTTCCCATGG CTTCTTGCAG CTCTCAGGAG GGGTCATCTC 
     1261 CATGCCATCT GTTGACAGTG AGGATCATTG GCATGAAAAA CGTCCGGCAG GCTGATATAC 
     1321 TGAGTCAGAC AGACTGCTTT GTGACCCTCT GGCTGCCTAC TGCCTCTCAG AAGAAGCTGA 
     1381 AGACCAGAAC CATCTCCAAC TGCCTACACC CAGAGTGGGA CGAAAGCTTC ACCTTTCAGA 
     1441 TCCAGACTCA AGTAAAGAAT GTGCTAGAGC TGAGCGTCTG TGACGAAGAC ACCCTGACAC 
     1501 AAAATGACCA TCTCTTGACA GTCCTCTATG ACCTCTCTAA GCTCTGCCTC CGGAATAAAA 
     1561 CCCATGTGAA GTTCCCACTC AACCCAGAGG GCATGGAAGA ACTGGAGGTG GAGTTCCTAC 
     1621 TCGAAGAGAA TTTCTCCTCA TCAGAGACCC TCATCACCAA CGGCGTGCTG GTGTCTCGCC 
     1681 AAGTCTCTTG CCTGGAGGTT CATGCAGAAT CCAGGAGGCC GAGGAAGAGG AAGAAAAACA 
     1741 AAGACCTTCT GGTGATGGTG ACAGACTCCT TCGAGAACAC CCAGCGTGTC CCGCCTTGCC 
     1801 GGGAGCCCTG CTACCCCAAT TCTGCCTGCT TCCACTACCC CAAGTACTCC CAGCCACAGC 
     1861 TTTACGCAGA GGCGCCTAAG AGCCACTGTA ACTTTAGGCT TTGCTGCTGC GGAACACACA 
     1921 GGAATGACCC TGTCTGCCAG CCCCTCAATT GCCTTTCTGA TGGCCAGGTG ACAACCCTGC 
     1981 CTGTGGGAGA GAACTATGAG CTACACATGA AGTCCTCACC CTGCTCTGAC ACACTGGATG 
     2041 TGCGGCTTGG ATTCAACCTG TGCCAGGAAG AGGTGGAGTT TGTGCAGAAG CGGAAGATGG 
     2101 TGGTGGCCAA GACACTAAGT CAGATGCTGC AGCTGGAGGA AGGCCTGCAT GAGGATGAGG 
     2161 TACCGATAAT AGCCATCATG GCCACAGGAG GTGGCACAAG GTCTATGGTC TCCTTGTATG 
     2221 GCCACCTGCT GGGGTTGCAG AAGCTGAACT TTCTGGACAC TTCTACTTAC ATCACCGGCT 
     2281 TGTCAGGTGC AACCTGGACT ATGGCTACCT TGTACAGTGA TCCTGAGTGG TCCTCCAAAA 
     2341 ACCTGGAGAC TGTTGTCTTT GAGGCCCGGA GACATGTTGT CAAAGACAAG ATGCCTGCCC 
     2401 TGTTCCCAGA TCAGCTCTAC AAATGGCGAG AGGACCTCCA AAAGCATAGC CAGGAGGGCT 
     2461 ATAAGACCAC GTTTACAGAC TTTTGGGGCA AGCTGATCGA GTACAGTCTG GGAGATAAAA 
     2521 AAAACGAATG CAAGCTGTCA GATCAGCGAG CTGCTCTGTG CCGGGGACAG AACCCTCTGC 
     2581 CCATCTACCT CACCATCAAT GTCAAGGATG ATGTAAGCAA CCAGGATTTC AGAGAATGGT 
     2641 TCGAGTTCTC CCCCTACGAG GTGGGCATGC AGAAGTACGG AGCCTTCATC CCCAGCGAGT 
     2701 TATTTGGCTC CGAGTTCTTC ATGGGGCGGC TGATGAAGAG GATTCCTGAG CCGGAGATGT 
     2761 GCTACATGCT AGGGTTGTGG AGTAGCATCT TTTCCCTGAA CCTGCTTGAT GCCTGGAATT 
     2821 TGTCTCACAC CTCAGAGGAG TTTTTCTATA GGTGGACAAG GGAGAGACTG CATGACATCG 
     2881 AAGATGATCC CATCCTGCCT GAAATCCCTA GGTGTGACGA TAACCCCCTA GAGACCACAG 
     2941 TAGTGATCCC AACGACATGG CTGTCCAACA CCTTCCGAGA AATCCTCACA CGCAGGCCCT 
     3001 TCGTGTCTGA GTTCCACAAC TTCCTGTACG GGATGCAGCT GCATACTGAC TACTTACAGA 
     3061 ACAGACAGTT CTCTATGTGG AAAGACACAG TACTGGACAC CTTCCCAAAC CAGCTGACAC 
     3121 AGTTTGCAAA ACACCTGAAC CTGCTGGACA CTGCGTTCTT TGTCAACTCC AGCTACGCAC 
     3181 CCCTCCTCAG GCCAGAGAGA AAAGTCGACC TTATCATCCA CCTCAATTAC TGCGCAGGAT 
     3241 CCCAGACAAA GCCCCTGAAA CAAACCTGTG AGTACTGTAC CGAGCAGAAG ATCCCCTTCC 
     3301 CCAGCTTCTC CATCCTGGAA GATGACAACA GTCTCAAGGA GTGCTACGTG ATGGAGAATC 
     3361 CCCAGGAGCC CGACGCCCCC ATCGTGGCTT ACTTCCCACT CATCAGTGAC ACCTTCCAGA 
     3421 AGTACAAGGC TCCAGGTGTA GAGCGAAGTC CTGACGAGCT GGAACTGGGC CAGCTGAACA 
     3481 TCTATGGACC AAAGTCTCCC TATGCCACCA AGGAGCTGAC GTACACAGAG GCCGCCTTCG 
     3541 ACAAGCTGGT GAAGCTCTCA GAATATAATA TCCTCAATAA CAGAGATAAG CTCATTCAGG 
     3601 CCTTGAGACT AGCAATGGAG AAGAAACGCA TGAGGAGCCA GTGTCCCTCC ACGCGTACGC 
     3661 GGCCGCTCGA GATGGAGAGC GACGAGAGCG GCCTGCCCGC CATGGAGATC GAGTGCCGCA 
     3721 TCACCGGCAC CCTGAACGGC GTGGAGTTCG AGCTGGTGGG CGGCGGAGAG GGCACCCCCG 
     3781 AGCAGGGCCG CATGACCAAC AAGATGAAGA GCACCAAAGG CGCCCTGACC TTCAGCCCCT 
     3841 ACCTGCTGAG CCACGTGATG GGCTACGGCT TCTACCACTT CGGCACCTAC CCCAGCGGCT 
     3901 ACGAGAACCC CTTCCTGCAC GCCATCAACA ACGGCGGCTA CACCAACACC CGCATCGAGA 
     3961 AGTACGAGGA CGGCGGCGTG CTGCACGTGA GCTTCAGCTA CCGCTACGAG GCCGGCCGCG 
     4021 TGATCGGCGA CTTCAAGGTG ATGGGCACCG GCTTCCCCGA GGACAGCGTG ATCTTCACCG 
     4081 ACAAGATCAT CCGCAGCAAC GCCACCGTGG AGCACCTGCA CCCCATGGGC GATAACGATC 
     4141 TGGATGGCAG CTTCACCCGC ACCTTCAGCC TGCGCGACGG CGGCTACTAC AGCTCCGTGG 
     4201 TGGACAGCCA CATGCACTTC AAGAGCGCCA TCCACCCCAG CATCCTGCAG AACGGGGGCC 
     4261 CCATGTTCGC CTTCCGCCGC GTGGAGGAGG ATCACAGCAA CACCGAGCTG GGCATCGTGG 
     4321 AGTACCAGCA CGCCTTCAAG ACCCCGGATG CAGATGCCGG TGAAGAAAGA GTTTAAACGG 
     4381 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4441 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4501 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4561 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4621 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4681 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4741 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4801 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4861 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4921 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4981 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5041 GGTACGCGGC CAGCTTGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 
     5101 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 
     5161 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5221 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 
     5281 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC 
     5341 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 
     5401 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 
     5461 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 
     5521 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 
     5581 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 
     5641 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 
     5701 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 
     5761 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 
     5821 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 
     5881 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 
     5941 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 
     6001 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 
     6061 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCCGA CCGCTATCAG 
     6121 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 
     6181 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 
     6241 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA 
     6301 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 
     6361 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 
     6421 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 
     6481 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 
     6541 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT 
     6601 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 
     6661 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 
     6721 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 
     6781 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 
     6841 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 
     6901 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 
     6961 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 
     7021 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 
     7081 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 
     7141 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 
     7201 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 
     7261 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 
     7321 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 
     7381 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 
     7441 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 
     7501 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC 
     7561 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 
     7621 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 
     7681 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 
     7741 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 
     7801 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 
     7861 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 
     7921 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 
     7981 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 
     8041 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 
     8101 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 
     8161 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 
     8221 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 
     8281 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 
     8341 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 
     8401 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 
     8461 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 
     8521 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 
     8581 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 
     8641 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 
     8701 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT 
     8761 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 
     8821 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 
     8881 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 
     8941 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 
     9001 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 
     9061 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT 
     9121 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 
     9181 TT
//