LOCUS       MG211382                9509 bp    DNA     circular  SYN 
DEFINITION  Eif4enif1 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 4E nuclear import factor 1 (Eif4enif1)
ACCESSION   NM_023743
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3990..4694 
                     /label="GFP"  
     misc_feature    4741..5326 
                     /label="PolyA signal"  
     misc_feature    7418..8063 
                     /label="ColE1"  
     misc_feature    5780..6574 
                     /label="Neo"  
     misc_feature    5411..5713 
                     /label="SV40 ori"  
     misc_feature    8078..8938 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3969..3975 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3969 
                     /label="Eif4enif1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAAAAGT GTGGCTGAAA CAGAAAATGG AGATGCTTTC CTTGAGCTGA 
     1081 AGAAACTGCC CACCTCCAAA TCCCCCCATC GCTATACGAA AGAGGAGCTC TTGGATATAA 
     1141 AAGAACGTCC ATACTCCAAG CAGAGGCCAT CATGCCTCTC TGAAAAATAT GACAGTGATG 
     1201 GTGTCTGGGA CCCTGAGAAG TGGCATGCCT CTCTCTACCC AGCCTCGGGG CGGAGCTCAC 
     1261 CAGTGGAAAG TCTGAAGAAA GAGTCAGAAT CCGATCGACC TTCTCTGGTG CGCAGAATAG 
     1321 CAGATCCACG AGAGCGAGTG AAAGAAGATG ACTTAGATGT TGTTCTCAGC CCACAGAGAC 
     1381 GAAGCTTTGG AGGTGGCTGC CATGTGACAG CTGCTGTTAG CTCCCGACGA TCTGGCAGTC 
     1441 CATTGGAGAA AGACAGTGAT GGACTTCGCC TGCTTGGTGG ACGAAGAATT GGCAGTGGAA 
     1501 GGATAATTTC TGCCCGGGCC TTTGAAAAGG ATCACCGTCT CAGTGATAAG GACCTGCGAG 
     1561 ACTTGAGAGA CCGAGACAGA GAGAGGGACT ACAAGGACAA ACGTTTCAGG AGGGAGTTTG 
     1621 GAGATAGTAA ACGTGTCTTT GGTGAGCGTA GAAGAAACGA TTCATACACT GAGGAAGAAC 
     1681 CAGAATGGTT TTCAGCTGGA CCCACCAGTC AGTCTGAAAC CATAGAGCTG ACTGGCTTTG 
     1741 ATGATAAGAT TCTAGAGGAG GATCACAAAG GCAGAAAAAG AACAAGGCGA CGGACTGCCT 
     1801 CTGTGAAGGA AGGCATAGTG GAGTGCAATG GAGGGGTGGC TGAAGAGGAC GAGGTGGAAG 
     1861 TCATTCTGGC CCAGGAGCCC TCTGCTGATC AGGAAGTTCC AAGGGATGTC ATCCTACCTG 
     1921 AGCAGTCCCC AGGAGAATTT GACTTTAATG AATTCTTTAA CCTTGATAAG GTGCCATGCT 
     1981 TGGCTTCGAT GATAGAAGAT GTTCTGGGGG AAGGATCGGT CTCTGCCAGC CGCTTCAGTA 
     2041 GGTGGTTCTC TAACCCAAGC CGGTCAGGGA GCAGATCCAG CAGCCTTGGA TCCACACCCC 
     2101 ATGAAGAACT TGAGAGACTG GCAGGTTTGG AGCAAGCCGT CCTCTCTCCT GGACAGAACT 
     2161 CGGGGAATTA TTTTGCTCCT ATACCATCGG AAGACCATGC TGAAAATAAA GTGGACATTT 
     2221 TAGAAATGCT ACAGAAAGCC AAAGTAGATT TAAAACCTCT TCTTTCTAGC CTTTCTGCAA 
     2281 ATAAAGAAAA ACTTAAGGAG AGCTCACATT CAGGGGTTGT ACTTTCAGTG GAAGAGGTAG 
     2341 AAGCAGGGCT GAAGGGCTTG AAAGTGGACC AGCAAATGAA GAATTCAACT CCCTTCATGG 
     2401 CAGAACATTT AGAGGAGACC CTGAGTGCTG CGAGTAGCAA TAGACAGCTC AAGAAAGATG 
     2461 GAGATATGAC TGCATTCAAC AAGCTAGTGA ACACCATGAA GGCAAGTGGA ACTTTGCCTA 
     2521 CCCAGCCCAA AGTCAGTCGA AACGTTGAAA GTCACTTATT GGCCCCTGCT GAGATTCCAG 
     2581 GCCAGCCTGT TTCTAAAAAC ATCCTACAGG AACTTCTGGG TCAACCGGTT CAGAGACCCG 
     2641 CTTCTTCCAA TCTTCTCAGT GGCCTTATGG GGAGCTTGGA GGCTACTGCA TCTTTATTGA 
     2701 GCCAAAGAGC GCCCTCTCCT CCAATGTCAC AGGTATTTCG AACTCAAGCA GCCTCAGCAG 
     2761 ACTACCTTCA TCCAAGGATA CCATCACCAA TAGGTTTCCC TTCAGGACCC CAGCAACTAC 
     2821 TTGGAGATCC ATTCCAAGGC ATGCGCAAGC CCATGAGTCC TGTCTCAGCC CAGATGAGCC 
     2881 AGCTAGAATT GCAGCAAGCT GCTTTGGAGG GGCTGGCCCT ACCACATGAC CTTGCAGTCC 
     2941 AGACAGCACC CTTCTACCAG CCTGGTTTTA GCAAACCACA AGTGGACAGA ACCAGAGATG 
     3001 GGCTCAGAAA CAGGCAACAG CGAATGTCAA AGTCACCAGC ACCCATGCAT GGTGGAAATT 
     3061 CCTCTTCCCC AGCTCCTGCT GCCTCCATCA CAAGCATGCT TTCTCCTTCC TTCACCCCTA 
     3121 CCTCAGTGAT CCGGAAGATG TATGAAAGCA GAGAGAAAAC CAAGGAGGAA ATGGCACCTG 
     3181 GAATGGTGGT TCCTGGTGAT GGTAAAGAGG ATACTCAGAA GACCAGCGAA GAGAACCTGC 
     3241 TGTCATCCAA TCCCATACCC AATACTGATC AAGACTCTTC TACGACAAAT CCTAAGCTTT 
     3301 CAACATTACA GCGGTCTTCA TGTTCCACCC CACTCTCCCA GACCAGCCGT TATACCAAAG 
     3361 AACAAGATTA TCGACCTAAA ACAGCAGGAA GGAAAACACC TACCTTGGCA TCCCCAGTCC 
     3421 CAGGAACACC TTTTCTCCGC CCTACCCACC AAGTTCCCCT TGTCCCTCAT GTTCCTATTG 
     3481 TTCGACCTGC ACACCAGCTT CACCCAGGAT TAGTCCAAAG GTTGATAGCC CAAGGGGTAC 
     3541 ATCCACAGCA TCTTCCAAGT TTGCTCCAAG CTGGTGTGCT TCCTCCTGGG ATAGACATGG 
     3601 CTCCTTTACA GGGGCTATCT GGCCCACTCC TGGGTCAACC CTTGTACCCT TTGGTCTCTG 
     3661 CTGCTAGTCA CCCTCTCCTA AACCCTCGTC CTGGGACCCC TCTGCATTTG GCGGTGATGC 
     3721 AGCAGCAGCT ACAGCGCTCA GTTCTGCATC CTCCAGGCTC TAGTTCCCAG GCAGCAGCTA 
     3781 TCAGCGTTCA GACTCCTCAG AATGTACCCA GCCGGTCGGG CATGCCCCAC ATGCACTCCC 
     3841 AGCTGGAGCA TCGTACCAGC CAAAGGAGCA GCTCCCCTGT GGGCCTTGCC AAATGGTTTG 
     3901 GCTCAGATGT GCTACAGCAG CCTCTGCCCT CCATGCCCAC CAAAGTCATC AGTGTAGATG 
     3961 AACTGGAATA TAGACAGACG CGTACGCGGC CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC 
     4021 TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC 
     4081 TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA 
     4141 CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT 
     4201 ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG 
     4261 GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT 
     4321 TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGATG GGCACCGGCT 
     4381 TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC 
     4441 ACCTGCACCC CATGGGCGAT AACGATCTGG ATGGCAGCTT CACCCGCACC TTCAGCCTGC 
     4501 GCGACGGCGG CTACTACAGC TCCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC 
     4561 ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGGATC 
     4621 ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCGGATGCAG 
     4681 ATGCCGGTGA AGAAAGAGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4741 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4801 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4861 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4921 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4981 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5041 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5101 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5161 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5221 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5281 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5341 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CTTGGCTGTG GAATGTGTGT 
     5401 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5461 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5521 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 
     5581 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 
     5641 TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 
     5701 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 
     5761 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 
     5821 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 
     5881 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 
     5941 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 
     6001 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 
     6061 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 
     6121 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 
     6181 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 
     6241 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 
     6301 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 
     6361 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 
     6421 CGGCTGGGTG TGGCCGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 
     6481 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 
     6541 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 
     6601 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 
     6661 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC 
     6721 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA 
     6781 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 
     6841 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA 
     6901 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 
     6961 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 
     7021 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 
     7081 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 
     7141 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 
     7201 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 
     7261 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 
     7321 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 
     7381 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 
     7441 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 
     7501 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 
     7561 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 
     7621 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 
     7681 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 
     7741 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 
     7801 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 
     7861 GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 
     7921 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 
     7981 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 
     8041 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 
     8101 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 
     8161 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 
     8221 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 
     8281 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 
     8341 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 
     8401 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 
     8461 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 
     8521 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 
     8581 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 
     8641 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 
     8701 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 
     8761 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 
     8821 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 
     8881 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 
     8941 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 
     9001 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 
     9061 CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 
     9121 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT 
     9181 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC 
     9241 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG 
     9301 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT 
     9361 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG 
     9421 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC 
     9481 TGATTTAACA AAAATTTAAC GCGAATTTT
//