LOCUS       MG211381                9503 bp    DNA     circular  SYN 
DEFINITION  Usp15 (tGFP-tagged) - Mouse ubiquitin specific peptidase 15 (Usp15)
ACCESSION   NM_027604
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3984..4688 
                     /label="GFP"  
     misc_feature    4735..5320 
                     /label="PolyA signal"  
     misc_feature    7412..8057 
                     /label="ColE1"  
     misc_feature    5774..6568 
                     /label="Neo"  
     misc_feature    5405..5707 
                     /label="SV40 ori"  
     misc_feature    8072..8932 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3963..3969 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3963 
                     /label="Usp15"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAAGGC GGAGCGGCGG ACCTGGACAC CCAGCGTTCT GACATCGCGA 
     1081 CGCTGCTCAA AACCTCGCTC CGGAAAGGGG ACACCTGGTA TCTAGTAGAT AGTCGGTGGT 
     1141 TCAAACAGTG GAAAAAATAT GTTGGCTTTG ACAGCTGGGA CAAATACCAG ATGGGAGATC 
     1201 AAAATGTCTA TCCTGGACCC ATCGATAACT CTGGACTTCT CAAAGATGGC GACGCGCAGT 
     1261 CACTTAAGGA GCACCTTATT GATGAGTTGG ATTACATACT CTTGCCAACT GAGGGCTGGA 
     1321 ATAAACTTGT CAGCTGGTAC ACACTGATGG AGGGCCAGGA GCCAATAGCA AGAAAGGTGG 
     1381 TAGAACAGGG TATGTTTGTA AAGCACTGCA AAGTAGAAGT CTACCTCACA GAACTGAAGC 
     1441 TCTGTGAGAA CGGGAACATG AACAATGTTG TAACTCGGAG GTTTAGCAAA GCTGACACAA 
     1501 TAGATACGAT TGAGAAGGAA ATAAGAAAAA TCTTCAATAT TCCAGATGAG AAGGAGGCCA 
     1561 GACTGTGGAA CAAGTATGTG AGCAACACAT TTGAACCACT GAATAAGCCA GACAGCACCA 
     1621 TCCAGGATGC CGGCCTATAC CAAGGACAGG TGTTAGTGAT AGAGCAAAAA AATGAAGATG 
     1681 GAACGTGGCC CAGGGGTCCC TCTACTCCTA AGTCCCCAGG TGCATCCAAT TTTTCAACTT 
     1741 TACCAAAGAT CTCTCCTTCA TCTCTATCAA ATAATTATAA CAACATCAAC AACAGAAATG 
     1801 TGAAAAACTC AAATTATTGT CTCCCGTCAT ATACTGCTTA TAAGAACTAT GATTATTCAG 
     1861 AACCTGGAAG AAACAATGAA CAGCCAGGCC TGTGTGGCTT AAGTAACTTG GGAAATACGT 
     1921 GTTTCATGAA CTCAGCCATT CAGTGTTTGA GCAACACACC TCCACTTACT GAATATTTCC 
     1981 TCAATGATAA GTACCAAGAA GAGCTGAATT TTGACAATCC CTTAGGAATG AGAGGTGAAA 
     2041 TAGCTAAATC ATACGCTGAA CTCATCAAGC AGATGTGGTC TGGAAAGTTT AGCTACGTCA 
     2101 CTCCAAGAGC ATTCAAGACA CAGGTAGGGC GCTTCGCACC TCAGTTCTCT GGATATCAGC 
     2161 AGCAGGACTG CCAAGAACTA CTGGCTTTCC TGTTGGATGG ACTGCACGAG GACCTGAACA 
     2221 GAATTAGGAA AAAACCCTAT ATCCAGTTAA AAGATGCTGA TGGCAGGCCA GACAAGGTGG 
     2281 TTGCTGAAGA AGCCTGGGAG AACCATTTAA AGCGAAATGA TTCCATCATA GTAGACATAT 
     2341 TCCATGGCCT TTTCAAGTCT ACGTTAGTGT GTCCTGAGTG TGCTAAGATC TCGGTGACCT 
     2401 TTGACCCTTT CTGTTACTTG ACTCTTCCAT TGCCTATGAA AAAAGAACGT AGCTTGGAAG 
     2461 TTTATTTAGT TAGAATGGAT CCACTTGCTA AACCTATGCA ATACAAAGTT ATTGTGCCCA 
     2521 AAATTGGAAA TATACTAGAT CTTTGTACAG CGCTGTCTGC TTTGTCGGGA GTACCTGCAG 
     2581 ATAAGATGAT AGTCACTGAC ATATACAATC ATAGATTTCA CAGGATATTT GCCGTGGATG 
     2641 AAAACCTGAG TAGTATTATG GAGCGGGATG ATATTTACGT GTTTGAAATT AACATCAACA 
     2701 GGGCAGAAGA CACAGAGCAT GTAGTCATTC CTGTTTGCCT AAGAGAAAAA TTCAGACACT 
     2761 CAAGTTACAC GCACCACACT GGCTCTTCAC TGTTCGGCCA GCCTTTTCTT ATGGCTATAC 
     2821 CACGAAACAA TACTGAAGAT AAACTCTATA ATCTTCTACT CTTGAGAATG TGCCGGTATG 
     2881 TCAAAATGTC TACTGAAACT GAGGAAACAG ATGGACACCT ACGTTGCTGT GAGGACCAGA 
     2941 ATATTAATGG GAATGGGCCA AATGGCCTAC ATGAAGAGGG TTCACCAAGT GAGATGGAAA 
     3001 CCGACGAGCC AGGTGACGAG TCCAGCCAGG ATCAAGAGCT TCCCTCAGAG AACGAGAACA 
     3061 GTCAGTCTGA AGATTCAGTC GGAGGAGATA ACGATTCTGA AAATGGATTG TGCACTGAAG 
     3121 AAACATGCAA AGGTCAACTT ACGGGACACA AAAAGCGCTT GTTTACATTC CAGTTCAACA 
     3181 ACTTAGGCAA CAATGACATC AACTACATCA AAGACGACAC CAGTCACATC CGATTCGATG 
     3241 ACAGGCAGCT TCGGCTGGAC GAAAGATCTT TTCTCGCTTT GGACTGGGAC CCTGACTTGA 
     3301 AGAAGAGATA CTTTGATGAG AATGCTGCAG AGGATTTTGA AAAACATGAA AGTGTGGAAT 
     3361 ACAAACCTCC AAAAAGGCCC TTTGTGAAGT TAAAAGACTG CATTGAGCTC TTCACCACCA 
     3421 AGGAGGAGTT GGGTGCTGAG GACCCCTGGT ATTGTCCAAA TTGTAAAGAG CATCAGCAAG 
     3481 CCACAAAGAA ATTAGATCTG TGGTCCCTGC CTCCAGTTCT TGTGGTGCAT CTCAAGAGAT 
     3541 TCTCCTATAG TCGCTACATG AGAGACAAGC TAGATACATT AGTCGATTTT CCCATCAGTG 
     3601 ACTTGGATAT GTCAGAATTC CTAATTAACC CAAATGCAGG TCCTTGCCGC TATAATTTGA 
     3661 TTGCTGTTTC CAACCACTAT GGAGGGATGG GAGGAGGACA TTATACTGCT TTTGCAAAAA 
     3721 ACAAAGATGA TGGGAAGTGG TACTATTTCG ATGACAGTAG CGTCTCCACT GCATCTGAAG 
     3781 ACCAAATCGT GTCCAAGGCA GCGTACGTGC TCTTCTACCA GAGACAGGAC ACTTTCAGTG 
     3841 GGACTGGCTT CTTCCCTCTT GACCGAGAAA CTAAAGGTGC TTCAGCTGCC ACAGGTATCC 
     3901 CCCTGGAAAG TGACGAAGAC AGCAATGATA ATGACAATGA CCTAGAAAAT GAAAACTGTA 
     3961 TGCACACTAA TACGCGTACG CGGCCGCTCG AGATGGAGAG CGACGAGAGC GGCCTGCCCG 
     4021 CCATGGAGAT CGAGTGCCGC ATCACCGGCA CCCTGAACGG CGTGGAGTTC GAGCTGGTGG 
     4081 GCGGCGGAGA GGGCACCCCC GAGCAGGGCC GCATGACCAA CAAGATGAAG AGCACCAAAG 
     4141 GCGCCCTGAC CTTCAGCCCC TACCTGCTGA GCCACGTGAT GGGCTACGGC TTCTACCACT 
     4201 TCGGCACCTA CCCCAGCGGC TACGAGAACC CCTTCCTGCA CGCCATCAAC AACGGCGGCT 
     4261 ACACCAACAC CCGCATCGAG AAGTACGAGG ACGGCGGCGT GCTGCACGTG AGCTTCAGCT 
     4321 ACCGCTACGA GGCCGGCCGC GTGATCGGCG ACTTCAAGGT GATGGGCACC GGCTTCCCCG 
     4381 AGGACAGCGT GATCTTCACC GACAAGATCA TCCGCAGCAA CGCCACCGTG GAGCACCTGC 
     4441 ACCCCATGGG CGATAACGAT CTGGATGGCA GCTTCACCCG CACCTTCAGC CTGCGCGACG 
     4501 GCGGCTACTA CAGCTCCGTG GTGGACAGCC ACATGCACTT CAAGAGCGCC ATCCACCCCA 
     4561 GCATCCTGCA GAACGGGGGC CCCATGTTCG CCTTCCGCCG CGTGGAGGAG GATCACAGCA 
     4621 ACACCGAGCT GGGCATCGTG GAGTACCAGC ACGCCTTCAA GACCCCGGAT GCAGATGCCG 
     4681 GTGAAGAAAG AGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4741 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4801 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4861 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4921 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4981 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5041 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5101 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5161 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5221 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5281 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5341 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCTTGGC TGTGGAATGT GTGTCAGTTA 
     5401 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     5461 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     5521 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 
     5581 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 
     5641 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 
     5701 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA 
     5761 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG 
     5821 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT 
     5881 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC 
     5941 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 
     6001 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG 
     6061 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA 
     6121 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA 
     6181 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT 
     6241 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 
     6301 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC 
     6361 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG 
     6421 GGTGTGGCCG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT 
     6481 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG 
     6541 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA 
     6601 TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT 
     6661 ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG 
     6721 GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT 
     6781 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 
     6841 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA 
     6901 GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA 
     6961 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 
     7021 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 
     7081 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 
     7141 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 
     7201 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 
     7261 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 
     7321 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 
     7381 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 
     7441 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 
     7501 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 
     7561 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 
     7621 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 
     7681 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 
     7741 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 
     7801 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 
     7861 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 
     7921 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 
     7981 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 
     8041 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG 
     8101 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG 
     8161 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 
     8221 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 
     8281 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG 
     8341 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG 
     8401 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT 
     8461 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 
     8521 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC 
     8581 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA 
     8641 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC 
     8701 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT 
     8761 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 
     8821 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA 
     8881 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA 
     8941 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 
     9001 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 
     9061 AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC 
     9121 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT 
     9181 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG 
     9241 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT 
     9301 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 
     9361 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 
     9421 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 
     9481 AACAAAAATT TAACGCGAAT TTT
//