LOCUS       MG211379                9503 bp    DNA     circular  SYN 
DEFINITION  Rnf111 (tGFP-tagged) - Mouse ring finger 111 (cDNA clone MGC:76599 IMAGE:30094289)
ACCESSION   BC069835
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3984..4688 
                     /label="GFP"  
     misc_feature    4735..5320 
                     /label="PolyA signal"  
     misc_feature    7412..8057 
                     /label="ColE1"  
     misc_feature    5774..6568 
                     /label="Neo"  
     misc_feature    5405..5707 
                     /label="SV40 ori"  
     misc_feature    8072..8932 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3963..3969 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3963 
                     /label="Rnf111"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTCAATGG ACTCCTGAAT TTAACGAGCT CTACACCCTA AAAGTGGCTA 
     1081 TGAAGAGTGG GACTCCTGAT GCGCCCACTA CACAGGAGAG TCTGAAGGCG GTCCTTCTGC 
     1141 ACCCACAGCC CCTAGGGGCC ACCAAGAGCT TCCCTGCAGA AGTGGAGATG ATTAATAGCA 
     1201 AAGTGGGGAA TGAATTCTCT CATCTGTGTG ATGATTCTCA AAAACAAGAG AAGGACATGA 
     1261 CTGGCAACCA GCAAGAACAA GAAAAAAGTG GTGTTGTGAG AAAAAAGCGC AAAAGCCAGC 
     1321 AGGCTGGCCC CTCATATGTG CAGAACTGTG TCAAAGAAAA TCAGGAAATC CTAGGGCGCA 
     1381 GACAACAGCT AGAGACACCG AGTGATGAGG ATAATGACTC TTCCTTAAGT GAGTGTCTGT 
     1441 CTTCTCCCTC ATCTAGTCTC CATTTTGGGG GCTCTGATAC AGTGACTTCA GATGAGGATA 
     1501 AAGAAGTCTC GGTGAGACAT ACCCAGCCTG TTCTGAGTGC TAAAAGTAGA AGTCACAGTG 
     1561 CAAGGTCTCA CAAGTGGCCT CGAACTGAGG CAGATCCTGT GCCAAGTTTG TTAATGAAAA 
     1621 GACCTTGTTT TCATGGCAGT GCACTAAGGA GAGTCACATG CAGAAAGAGG CTGGTGAAGA 
     1681 GCAGCTCCTC GCAGAGGACA CAGAAGCAGA AGGAGAGGAT GCTAGTGCAG AGGAAGAAGC 
     1741 GGGAGGCGCT AGCTCAGAGG AAGTATGCTC TTCTCTCCAG CTCCAGTAGC TCCAGTGAGA 
     1801 ATGACCTCAG CAGCGATTCC TCGTCCAGCT CCTCCACCGA TGGAGAAGAG GACCTGTGTG 
     1861 CATCTGCCAG TGAGAACCCC AGCAACCCCG CTGCTCCCTC AGGAAGTATT GATGAAGATG 
     1921 TTGTGGTGAT AGAAGCCTCC TTCACTCCCC AGGTCACTGC CAATGAAGAA ATTAATGTTA 
     1981 CCTCAACTGA CAGTGAAGTG GAGATTGTTA CAGTTGGAGA AAGCTATCGG TCTCGTTCAA 
     2041 CCCTTGGACA CTCCAGATCT CATTGGAGCC AAGGTTCAAG TTCGCATACA GGACGGCCAC 
     2101 AGGAGTCTCG AAACCGCAGT AGGATCTCTA CTGTTATACA GCCCCTGAGG CAGAATGCAG 
     2161 CAGAAGTTGT GGACCTTACT GTTGATGAAG ATGAACCTAC TATAGTACCA ACCACTTCTG 
     2221 CTAGAATGGA CTCACAAACT ACTAGTGCTT CCATTAACAA TTCAAATCCA TCTACCTCTG 
     2281 AGCAAGCCTC TGATACTACT TCAACTGTGG CCAGTAGCCA GCCTTCCACA GTGTCAGAGA 
     2341 CTGAAGCTAC TCTTACAAGC AATAGTGCAA CTGGTTCTTC TGTGGGCGAT GACGTGAGGA 
     2401 GAACTGCATC TAGTGCTGTC CCAGAAAGTG GTCCTCCTGC AATGCCAAGG CTACCTTCTT 
     2461 GCTGCCCCCA GCACTCACCA TGTGGAGGGA CATCACAGAG CCATCATGCA TTAGCACACC 
     2521 CACACTCAAG TTGTTTTCAG CAGCATGGCC ACCATTTTCA GCATCACCAC CATCACCACC 
     2581 ATACTCCCCA CCCAGCTGTC CCAGTTTCTC CTTCCTTCAG TGACCCTGCT TGCCCAGTGG 
     2641 AAAGACCTCA AGTACAGGCG CCTTGTGGAG CAAATAGCAG CTCTGGTTCC AGCTACCATG 
     2701 ACCAGCAGGC ATTGCCAGTC GATCTGAGCA ACAGTGCTCT CAGGACTCAT GGAAGTGGGG 
     2761 GTTTCCATGG TGCATCTGCT TTTGACCCCT GCTGCCCTGT CACATCTTCC CGGGCTGCAG 
     2821 TCTTTGGCCA TCAGGCTGCT GCTGCCCCGA CTCAGCCATT ATCAATAGAT GGCTATGGCT 
     2881 CCAGCATGGT TGCCCAGCCC CAGCCCCAGC CCCCTCCACA GCCTTCTCTT TCGTCATGTC 
     2941 GGCACTACAT GCCACCTCCG TATGCTTCCT TGACAAGACC ACTGCACCAT CAAGCCTCAG 
     3001 CTTGCCATCA CTCTCATGGA AATGCCCCTC CCCAGACTCA GCCTCCCCCT CAAGTGGATT 
     3061 ACGTGATTCC GCATCCTGTC CATGCTTTCC ATTCTCAGAT ATCTTCTCAT GCAGCATCTC 
     3121 ACCCTGTGGC ACCACCGCCG CCAACACATT TAGGCAGTAC AGCGGCACCC ATCCCTCAGC 
     3181 ATCTTCCTCC TGCTCACCAG CCGATTTCAC ACCATATTCC AGCCCCAGCA CCTTCAGCCC 
     3241 AGAGACTGCA CCCTCATGAA GTGATGCAGA GGATGGAAGT TCAGAGGAGG AGGATGATGC 
     3301 AGCATCCAAC GCGGGCACAT GAACGGCCCC CGCCCCATCC ACATAGGATG CACCCAAACT 
     3361 ATGGCCATGG GCATCATATT CATGTGCCTC AAACCATGTC TTCACATCCT CGCCAGGCTC 
     3421 CAGAGAGAAC TGCCTGGGAA CTGGGGATTG AAGCTGGAGT AACTGCAGCT ACCTACACAC 
     3481 CTGGTGCTCT GCACCCTCAC CTGGCCCATT ACCACGCCCC GCCTCGACTT CATCACCTCC 
     3541 AGTTAGGAGC ACTTCCTTTA ATGGTTCCTG ACATGGCAGG CTATCCTCAC ATCCGTTATA 
     3601 TTTCTTCAGG ATTGGATGGA GCATCATTCA GAGGTCCTTT TAGGGGCAAT TTTGAGGAAT 
     3661 TGATTCATCT GGAAGAAAGA TTAGGAAATG TCAATCGTGG AGCCTCCCAG GGAACAATTG 
     3721 AAAGATGCAC ATATCCACAT AAATATAAAA AGAGGAAACT GCACTGCAAA CAAGATGGGG 
     3781 AAGAAGGGAC TGAGGAAGAC ACAGAGGAAA AATGTACTAT CTGTTTGTCT ATTTTAGAGG 
     3841 AAGGTGAAGA TGTGAGGCGC CTTCCATGTA TGCACCTTTT CCACCAAGTG TGTGTTGATC 
     3901 AGTGGCTGAT CACCAATAAG AAGTGCCCCA TATGCAGAGT GGACATCGAG GCCCAGCTGC 
     3961 CAAGTGAAAG TACGCGTACG CGGCCGCTCG AGATGGAGAG CGACGAGAGC GGCCTGCCCG 
     4021 CCATGGAGAT CGAGTGCCGC ATCACCGGCA CCCTGAACGG CGTGGAGTTC GAGCTGGTGG 
     4081 GCGGCGGAGA GGGCACCCCC GAGCAGGGCC GCATGACCAA CAAGATGAAG AGCACCAAAG 
     4141 GCGCCCTGAC CTTCAGCCCC TACCTGCTGA GCCACGTGAT GGGCTACGGC TTCTACCACT 
     4201 TCGGCACCTA CCCCAGCGGC TACGAGAACC CCTTCCTGCA CGCCATCAAC AACGGCGGCT 
     4261 ACACCAACAC CCGCATCGAG AAGTACGAGG ACGGCGGCGT GCTGCACGTG AGCTTCAGCT 
     4321 ACCGCTACGA GGCCGGCCGC GTGATCGGCG ACTTCAAGGT GATGGGCACC GGCTTCCCCG 
     4381 AGGACAGCGT GATCTTCACC GACAAGATCA TCCGCAGCAA CGCCACCGTG GAGCACCTGC 
     4441 ACCCCATGGG CGATAACGAT CTGGATGGCA GCTTCACCCG CACCTTCAGC CTGCGCGACG 
     4501 GCGGCTACTA CAGCTCCGTG GTGGACAGCC ACATGCACTT CAAGAGCGCC ATCCACCCCA 
     4561 GCATCCTGCA GAACGGGGGC CCCATGTTCG CCTTCCGCCG CGTGGAGGAG GATCACAGCA 
     4621 ACACCGAGCT GGGCATCGTG GAGTACCAGC ACGCCTTCAA GACCCCGGAT GCAGATGCCG 
     4681 GTGAAGAAAG AGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4741 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4801 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4861 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4921 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4981 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5041 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5101 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5161 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5221 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5281 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5341 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCTTGGC TGTGGAATGT GTGTCAGTTA 
     5401 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     5461 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     5521 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 
     5581 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 
     5641 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 
     5701 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA 
     5761 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG 
     5821 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT 
     5881 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC 
     5941 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 
     6001 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG 
     6061 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA 
     6121 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA 
     6181 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT 
     6241 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 
     6301 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC 
     6361 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG 
     6421 GGTGTGGCCG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT 
     6481 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG 
     6541 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA 
     6601 TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT 
     6661 ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG 
     6721 GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT 
     6781 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 
     6841 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA 
     6901 GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA 
     6961 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 
     7021 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 
     7081 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 
     7141 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 
     7201 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 
     7261 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 
     7321 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 
     7381 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 
     7441 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 
     7501 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 
     7561 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 
     7621 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 
     7681 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 
     7741 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 
     7801 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 
     7861 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 
     7921 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 
     7981 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 
     8041 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG 
     8101 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG 
     8161 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 
     8221 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 
     8281 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG 
     8341 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG 
     8401 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT 
     8461 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 
     8521 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC 
     8581 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA 
     8641 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC 
     8701 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT 
     8761 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 
     8821 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA 
     8881 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA 
     8941 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 
     9001 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 
     9061 AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC 
     9121 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT 
     9181 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG 
     9241 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT 
     9301 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 
     9361 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 
     9421 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 
     9481 AACAAAAATT TAACGCGAAT TTT
//