LOCUS       MG211315                9440 bp    DNA     circular  SYN 
DEFINITION  Fgd1 (tGFP-tagged) - Mouse FYVE, RhoGEF and PH domain containing 1 (Fgd1)
ACCESSION   NM_008001
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3921..4625 
                     /label="GFP"  
     misc_feature    4672..5257 
                     /label="PolyA signal"  
     misc_feature    7349..7994 
                     /label="ColE1"  
     misc_feature    5711..6505 
                     /label="Neo"  
     misc_feature    5342..5644 
                     /label="SV40 ori"  
     misc_feature    8009..8869 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3900..3906 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3900 
                     /label="Fgd1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCATGGCCAC CGAGTCCCGG GGGGCCCTGG GCCTTCGGAT CCCGAACGCT 
     1081 CAGCGGCAAA TACCCCCGGT GCCGCTCCGC TGGCCTGTGC CGACTCGGAC CCTGGAGCCT 
     1141 TGGAACCCGG GCTGCCGGTG AGCAGGGGCT CAGGCACGGC TCTTGGAGGC CCTTTGGATC 
     1201 CTCAGTTTGT TGGACCCTCG GATGCCAGCC TGGGAGCGCC TCCAAGCTCC CGGGTCTTAC 
     1261 CCTGCGGCCC TAGTCCTCAG CACCACCGGG CCCTGCGCTT CTCCTACCAC CTGGAGGGCT 
     1321 CGCAGCCTCG GCCTGGACTG CACCAGGGAA ACCGGATCCT GGTTAAAAGT TTGTCCCTTG 
     1381 ACCCTGGCCA GAGCCTTGAG CCTCATCCAG AAGGTCCCCA ACGGCTTCGC TCAGATCCAG 
     1441 GCCCTCCCAC TGAAATCCCT GGCCCACGTC CTTCACCACT GAAGCGGGCA CCGGGCCCAA 
     1501 AGCCACAGGT TCCCCCAAAG CCCAGCTACC TGCAGATGCC CCGGGTGCTT CCTCCTCCTG 
     1561 AGCCCATTCC TCCACCACCA TCACGCCCTC TGCCTGCAGA CCCTAGAGTG GCCAAGGGAC 
     1621 TCGTCCCCAG AGCTGAGGCC AGCACTAGTT CTGCAGCAGT ATCATCATTG ATTGAGAAGT 
     1681 TTGAAAGAGA GCCTGTGATT GTTGCCTCGG ATAGGCCAGC CCCTGGCCCC TGCCCAGTTC 
     1741 CCCCAGAGCC AGCCATGTTG CCACAGCCAC CCCCGCAGCC AACAGGGTCC CAGCTCCCTG 
     1801 AGGGTGAAGC TTCTCGCTGC CTGTTCCTGC TGGCTCCTGG GCCCCGGGAT GGTGAGAAGG 
     1861 TGCCTAACCG GGACAGCGGC ATTGACAGCA TCAGCTCGCC ATCCAACAGT GAAGAGACCT 
     1921 GCTTCGTCAG TGATGATGGG CCCCCCATCC ACAGCCTCTG TCCTGGGCCC CCCGCCCTGG 
     1981 CTAGTATGCC AGTAGCTTTG GCCGACCCCC ACCGGCCTGG CTCCCAGGAG GTTGACAGTG 
     2041 ACCTGGAAGA GGAAGAGGAA GAAGAAGAGG AGGAAAAGGA AAGAGAAATT CCAGTGCCCC 
     2101 CGATGGAGAG ACAGGAGTCT GTGGAGCTGA CTGTGCAGCA GAAGGTGTTC CATATTGCCA 
     2161 ATGAGCTCCT GCAAACTGAG AAGGCCTATG TTTCCAGGCT CCATCTCCTG GATCAGGTAT 
     2221 TCTGTGCCCG GCTGCTGGAA GAAGCTCGGA ACCGCAGTTC CTTCCCCGCA GATGTTGTCC 
     2281 ACGGCATCTT CTCTAACATC TGCTCCATCT ATTGCTTCCA CCAGCAGTTC CTGCTGCCTG 
     2341 AGCTAGAGAA GCGCATGGAG GAATGGGACC GCTACCCACG CATTGGTGAC ATTCTTCAGA 
     2401 AGTTGGCACC CTTCCTCAAG ATGTATGGCG AGTATGTGAA AAACTTCGAC CGGGCGGTAG 
     2461 AGCTGGTCAA CACATGGACA GAGCGGTCTA CCCAGTTTAA AGTCATCATC CATGAAGTAC 
     2521 AGAAGGAGGA AGCCTGTGGC AACCTGACCT TGCAGCACCA CATGCTGGAG CCCGTGCAGC 
     2581 GTATCCCCCG CTATGAACTT CTTCTCAAGG ACTATCTGTT AAAGCTGCCT CATGGCTCCC 
     2641 CGGACAGCAA GGATGCCCAG AAGTCTTTGG AGCTGATAGC CACAGCAGCA GAACACTCTA 
     2701 ATGCTGCCAT CCGGAAAATG GAACGAATGC ACAAGCTGCT GAAGGTGTAT GAGCTGCTAG 
     2761 GGGGTGAGGA GGACATTGTC AGTCCCACCA AAGAGCTCAT AAAGGAAGGC CATATCCTTA 
     2821 AGCTGTCAGC AAAGAATGGG ACCACTCAAG ATCGATACCT CATATTATTC AACGACCGCC 
     2881 TCCTTTACTG CGTGCCCAGG CTGCGGCTCC TTGGTCAGAA GTTTAGCGTG CGGGCACGCA 
     2941 TTGATGTAGA TGGCATGGAG CTAAAGGAGA GCTCCAACCT CAACATGCCT CGGACCTTCC 
     3001 TGGTGTCAGG AAAGCAGCGC TCCCTCGAAC TGCAGGCCAG GACTGAGGAG GAGAAGAAAG 
     3061 ACTGGGTCCA GGCCATCAAT TCCACCCTCC TGAAGCATGA ACAGACCCTG GAGACTTTCA 
     3121 AACTGTTGAA CTCAACAAAC AGGGATGATG AAGATACTCC CCCTAACTCT CCAAACGTGG 
     3181 ATCTTGGGAA GAGGGCACCT ACGCCTATCC GGGAAAAGGA AGTCACCATG TGCATGCGCT 
     3241 GCCAGGAGCC CTTCAATTCC ATCACCAAAC GCAGGCACCA CTGCAAGGCC TGTGGGCATG 
     3301 TGGTTTGTGG GAAATGCTCT GAGTTCCGAG CCCGACTCAT CTATGACAAC AATCGTTCCA 
     3361 ACCGTGTGTG CACTGATTGC TATGTGGCCT TGCATGGAGC ACCTGGGAGC AGTCCAGCCT 
     3421 GTAGCCAGCA TACACCCCAG CGCAGGAGGT CCATCCTAGA GAAACAGGCC TCGGTCGCTG 
     3481 CTGAGAACAG CGTCATCTGC AGCTTCCTGC ACTACATGGA GAAGGGTGGG AAAGGATGGC 
     3541 ACAAGGCATG GTTTGTGGTT CCTGAGAATG AACCCTTGGT GCTGTACATC TATGGAGCCC 
     3601 CTCAGGATGT GAAAGCCCAG CGCAGCCTGC CCCTCATTGG CTTTGAGGTG GGGCCTCCTG 
     3661 AGGCAGGAGA GCGGCCAGAC AGGAGGCATG TCTTCAAGAT CACACAAAGC CACCTAAGCT 
     3721 GGTACTTCAG CCCAGAGACA GAAGAACTCC AGCGGCGCTG GATGGCTGTT CTCGGCAGGG 
     3781 CGGGCCGTGG GGACACATTC TGCCCAGGGC CCACACTGTC TGAGGACAAG GAGATGGAGG 
     3841 AGACACCAGT GGCAGCCTCA GGAGCCACTG CTGAACCTCC TGAAGCCTCC CAGACCCGAG 
     3901 ACAAGACCAC GCGTACGCGG CCGCTCGAGA TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA 
     3961 TGGAGATCGA GTGCCGCATC ACCGGCACCC TGAACGGCGT GGAGTTCGAG CTGGTGGGCG 
     4021 GCGGAGAGGG CACCCCCGAG CAGGGCCGCA TGACCAACAA GATGAAGAGC ACCAAAGGCG 
     4081 CCCTGACCTT CAGCCCCTAC CTGCTGAGCC ACGTGATGGG CTACGGCTTC TACCACTTCG 
     4141 GCACCTACCC CAGCGGCTAC GAGAACCCCT TCCTGCACGC CATCAACAAC GGCGGCTACA 
     4201 CCAACACCCG CATCGAGAAG TACGAGGACG GCGGCGTGCT GCACGTGAGC TTCAGCTACC 
     4261 GCTACGAGGC CGGCCGCGTG ATCGGCGACT TCAAGGTGAT GGGCACCGGC TTCCCCGAGG 
     4321 ACAGCGTGAT CTTCACCGAC AAGATCATCC GCAGCAACGC CACCGTGGAG CACCTGCACC 
     4381 CCATGGGCGA TAACGATCTG GATGGCAGCT TCACCCGCAC CTTCAGCCTG CGCGACGGCG 
     4441 GCTACTACAG CTCCGTGGTG GACAGCCACA TGCACTTCAA GAGCGCCATC CACCCCAGCA 
     4501 TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT TCCGCCGCGT GGAGGAGGAT CACAGCAACA 
     4561 CCGAGCTGGG CATCGTGGAG TACCAGCACG CCTTCAAGAC CCCGGATGCA GATGCCGGTG 
     4621 AAGAAAGAGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4681 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4741 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4801 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4861 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4921 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4981 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5041 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5101 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5161 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5221 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5281 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCTTGGCTGT GGAATGTGTG TCAGTTAGGG 
     5341 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5401 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5461 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT 
     5521 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 
     5581 GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 
     5641 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG 
     5701 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 
     5761 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 
     5821 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 
     5881 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG 
     5941 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 
     6001 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 
     6061 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 
     6121 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 
     6181 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG 
     6241 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG 
     6301 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 
     6361 GTGGCCGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 
     6421 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 
     6481 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA 
     6541 CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG 
     6601 AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG 
     6661 ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 
     6721 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 
     6781 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT 
     6841 AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA 
     6901 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA 
     6961 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT 
     7021 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT 
     7081 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT 
     7141 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 
     7201 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 
     7261 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 
     7321 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 
     7381 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 
     7441 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 
     7501 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 
     7561 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 
     7621 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 
     7681 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 
     7741 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTT 
     7801 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA 
     7861 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 
     7921 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 
     7981 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG 
     8041 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT 
     8101 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG 
     8161 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 
     8221 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 
     8281 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 
     8341 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 
     8401 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 
     8461 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 
     8521 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 
     8581 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 
     8641 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 
     8701 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 
     8761 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 
     8821 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 
     8881 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 
     8941 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 
     9001 TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA 
     9061 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT 
     9121 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT 
     9181 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC 
     9241 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT 
     9301 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT 
     9361 TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC 
     9421 AAAAATTTAA CGCGAATTTT 
//