LOCUS       MG211254                9374 bp    DNA     circular  SYN 
DEFINITION  Ap2a2 (tGFP-tagged) - Mouse adaptor protein complex AP-2, alpha 2 subunit (Ap2a2)
ACCESSION   NM_007459
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3855..4559 
                     /label="GFP"  
     misc_feature    4606..5191 
                     /label="PolyA signal"  
     misc_feature    7283..7928 
                     /label="ColE1"  
     misc_feature    5645..6439 
                     /label="Neo"  
     misc_feature    5276..5578 
                     /label="SV40 ori"  
     misc_feature    7943..8803 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3834..3840 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3834 
                     /label="Ap2a2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGCCGTA TCCAAGGGGG ACGGGATGCG AGGCCTAGCG GTCTTCATCT 
     1081 CCGACATCCG CAACTGTAAA AGTAAAGAAG CTGAAATAAA GAGAATAAAC AAAGAACTGG 
     1141 CAAATATTAG ATCCAAATTT AAAGGTGACA ACGCTCTTGA TGGCTACAGT AAAAAGAAGT 
     1201 ATGTCTGCAA ATTGCTCTTC ATCTTTCTCC TTGGTCATGA CATTGACTTT GGACACATGG 
     1261 AAGCTGTGAA TCTTCTGAGC TCAAACAGAT ACACGGAAAA GCAGATTGGC TACCTTTTCA 
     1321 TCTCTGTATT GGTGAACTCG AATAGTGAAC TGATCCGGTT GATTAACAAT GCCATCAAGA 
     1381 ATGACCTGGC CAGCCGCAAT CCTACGTTTA TGGGTCTCGC CCTGCACTGC ATTGCCAATG 
     1441 TGGGTAGCCG TGAGATGGCG GAGGCCTTCG CCGGAGAGAT CCCCAAGATC CTCGTGGCAG 
     1501 GAGATACCAT GGATAGTGTG AAGCAGAGCG CAGCCCTGTG CTTGTTACGC CTGTACAGGA 
     1561 CATCACCTGA CCTAGTTCCC ATGGGCGACT GGACATCTCG TGTGGTACAC CTCCTCAATG 
     1621 ACCAGCACTT GGGGGTAGTG ACTGCAGCGA CTAGCCTCAT CACCACCCTG GCCCAGAAGA 
     1681 ACCCTGAGGA GTTCAAGACC TCTGTCTCGC TAGCTGTCTC GCGACTGAGC AGGATTGTGA 
     1741 CGTCTGCATC AACAGATCTT CAGGATTATA CTTACTACTT TGTCCCGGCT CCTTGGCTGT 
     1801 CAGTCAAGCT TCTGAGACTG CTGCAGTGCT ACCCACCCCC AGACCCTGCG GTGCGTGGCC 
     1861 GCCTGACTGA GTGCTTGGAG ACCATCCTGA ACAAGGCCCA GGAGCCACCC AAGTCCAAGA 
     1921 AGGTCCAGCA TTCAAATGCC AAGAACGCTG TGCTGTTTGA GGCCATCAGC CTGATCATCC 
     1981 ACCATGACAG TGAGCCAAAC TTGCTCGTCC GCGCCTGCAA CCAGCTGGGC CAGTTCCTGC 
     2041 AGCACCGGGA GACGAACCTA CGCTACCTGG CTCTGGAGAG CATGTGCACG CTGGCCAGCT 
     2101 CTGAGTTCTC CCACGAGGCC GTCAAGACCC ACATTGAGAC GGTCATCAAT GCCTTGAAGA 
     2161 CGGAAAGAGA CGTGAGTGTG AGGCAGCGGG CAGTGGACCT GCTCTACGCC ATGTGTGACC 
     2221 GGAGCAACGC CCAGCAGATT GTGGCTGAGA TGCTGAGCTA CCTAGAAACG GCTGACTACT 
     2281 CCATCCGAGA GGAAATTGTG CTGAAGGTGG CCATCCTGGC TGAGAAGTAT GCAGTGGACT 
     2341 ACACTTGGTA TGTGGACACC ATCCTCAACC TCATCCGCAT CGCGGGCGAC TATGTGAGCG 
     2401 AGGAGGTGTG GTACCGCGTC ATCCAGATTG TCATCAATCG CGATGATGTG CAGGGCTATG 
     2461 CTGCCAAGAC GGTATTTGAG GCATTGCAGG CGCCAGCATG TCATGAGAAC TTGGTCAAAG 
     2521 TGGGCGGCTA CATCCTGGGG GAGTTTGGAA ACTTGATAGC TGGGGACCCA CGATCCAGCC 
     2581 CTCTGATCCA GTTCAACCTG CTCCACTCCA AGTTCCACTT GTGCAGCGTC CCCACTCGGG 
     2641 CACTCCTGCT GTCCACCTAC ATCAAGTTCG TGAACCTCTT TCCAGAGGTA AAAGCTACCA 
     2701 TTCAGGACGT GCTGCGCAGT GACAGCCAGC TGAAGAATGC CGACGTGGAG CTGCAGCAGC 
     2761 GGGCTGTGGA GTACCTGCGA CTGAGCACTG TCGCCAGCAC TGACATCCTG GCAACTGTCC 
     2821 TGGAGGAAAT GCCACCCTTC CCTGAGCGTG AGTCCTCCAT CTTGGCCAAG CTGAAGAAGA 
     2881 AGAAGGGCCC AAGCACAGTG ACTGACCTGG AGGAGACCAA GCGGGAGCGA AGCATCGATG 
     2941 TGAATGGGGG CCCTGAACCC GTTCCAGCCA GCACCAGTGC TGCGTCCACA CCTTCTCCGT 
     3001 CAGCAGACCT GCTGGGTCTG GGGGCCGTGC CCCCTGCTCC CACGGGGCCC CCTCCCTCCT 
     3061 CTGGCGGCGG GCTGCTGGTG GACGTGTTCT CAGACTCAGC TTCTGCAGTC GCACCTCTTG 
     3121 CACCCGGCTC TGAAGACAAC TTTGCCAGGT TTGTTTGTAA AAATAATGGT GTATTGTTCG 
     3181 AAAACCAGCT GCTTCAAATT GGACTTAAGT CAGAATTTCG GCAGAATTTA GGCCGAATGT 
     3241 TCATATTTTA TGGTAACAAG ACCTCCACGC AGTTCCTAAA CTTCACTCCA ACACTAATCT 
     3301 GTGCTGACGA CCTTCAAACT AATCTGAACC TGCAGACTAA GCCCGTGGAC CCAACTGTGG 
     3361 ATGGGGGCGC ACAGGTGCAA CAAGTGGTCA ACATTGAGTG TATATCTGAT TTCACAGAGG 
     3421 CACCTGTCCT CAACATCCAG TTCAGGTATG GTGGGACCTT CCAGAATGTG TCTGTTAAGC 
     3481 TTCCCATCAC TCTCAACAAA TTTTTCCAGC CCACAGAAAT GGCTTCTCAG GATTTCTTTC 
     3541 AACGTTGGAA GCAGTTGAGC AATCCACAGC AAGAAGTGCA GAATATCTTC AAAGCAAAGC 
     3601 ATCCGATGGA CACAGAGATC ACTAAGGCAA AGATTATTGG ATTTGGTTCT GCGCTCCTGG 
     3661 AAGAAGTTGA CCCGAATCCT GCAAATTTCG TGGGTGCTGG CATAATACAC ACCAAAACCA 
     3721 CCCAGATTGG CTGCTTGTTG CGCCTGGAGC CAAACCTGCA AGCTCAGATG TATAGACTCA 
     3781 CCTTGCGTAC CAGCAAAGAC ACCGTCTCTC AGAGACTATG TGAATTGCTG TCGGAACAGT 
     3841 TCACGCGTAC GCGGCCGCTC GAGATGGAGA GCGACGAGAG CGGCCTGCCC GCCATGGAGA 
     3901 TCGAGTGCCG CATCACCGGC ACCCTGAACG GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG 
     3961 AGGGCACCCC CGAGCAGGGC CGCATGACCA ACAAGATGAA GAGCACCAAA GGCGCCCTGA 
     4021 CCTTCAGCCC CTACCTGCTG AGCCACGTGA TGGGCTACGG CTTCTACCAC TTCGGCACCT 
     4081 ACCCCAGCGG CTACGAGAAC CCCTTCCTGC ACGCCATCAA CAACGGCGGC TACACCAACA 
     4141 CCCGCATCGA GAAGTACGAG GACGGCGGCG TGCTGCACGT GAGCTTCAGC TACCGCTACG 
     4201 AGGCCGGCCG CGTGATCGGC GACTTCAAGG TGATGGGCAC CGGCTTCCCC GAGGACAGCG 
     4261 TGATCTTCAC CGACAAGATC ATCCGCAGCA ACGCCACCGT GGAGCACCTG CACCCCATGG 
     4321 GCGATAACGA TCTGGATGGC AGCTTCACCC GCACCTTCAG CCTGCGCGAC GGCGGCTACT 
     4381 ACAGCTCCGT GGTGGACAGC CACATGCACT TCAAGAGCGC CATCCACCCC AGCATCCTGC 
     4441 AGAACGGGGG CCCCATGTTC GCCTTCCGCC GCGTGGAGGA GGATCACAGC AACACCGAGC 
     4501 TGGGCATCGT GGAGTACCAG CACGCCTTCA AGACCCCGGA TGCAGATGCC GGTGAAGAAA 
     4561 GAGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4621 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4681 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4741 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4801 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4861 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4921 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4981 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5041 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5101 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5161 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5221 CCTGTTGAAT TGGGTACGCG GCCAGCTTGG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA 
     5281 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5341 ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     5401 AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC 
     5461 AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG 
     5521 GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC 
     5581 TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA 
     5641 TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG 
     5701 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC 
     5761 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT 
     5821 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT 
     5881 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC 
     5941 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC 
     6001 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC 
     6061 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG 
     6121 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG 
     6181 GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT 
     6241 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCC 
     6301 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA 
     6361 TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC 
     6421 TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC 
     6481 AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT 
     6541 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA 
     6601 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA 
     6661 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT 
     6721 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT 
     6781 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC 
     6841 ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC 
     6901 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC 
     6961 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG 
     7021 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 
     7081 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 
     7141 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 
     7201 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 
     7261 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 
     7321 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 
     7381 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 
     7441 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 
     7501 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 
     7561 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 
     7621 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 
     7681 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTTTTTTTG 
     7741 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 
     7801 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 
     7861 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT 
     7921 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA 
     7981 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA 
     8041 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC 
     8101 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA 
     8161 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG 
     8221 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG 
     8281 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG 
     8341 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG 
     8401 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC 
     8461 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT 
     8521 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA 
     8581 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA 
     8641 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA 
     8701 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC 
     8761 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC 
     8821 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 
     8881 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 
     8941 CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA 
     9001 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG 
     9061 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT 
     9121 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG 
     9181 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA 
     9241 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT 
     9301 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT 
     9361 TTAACGCGAA TTTT
//