LOCUS       MG211247                9365 bp    DNA     circular  SYN 
DEFINITION  Pde2a (tGFP-tagged) - Mouse phosphodiesterase 2A, cGMP-stimulated (Pde2a)
ACCESSION   NM_001008548
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3846..4550 
                     /label="GFP"  
     misc_feature    4597..5182 
                     /label="PolyA signal"  
     misc_feature    7274..7919 
                     /label="ColE1"  
     misc_feature    5636..6430 
                     /label="Neo"  
     misc_feature    5267..5569 
                     /label="SV40 ori"  
     misc_feature    7934..8794 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3825..3831 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3825 
                     /label="Pde2a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCAGGCA TGCGGCCACT CCATCCTCTG CAGGAGCCAG CAGTACCCGG 
     1081 CAGCGCGACC GGCTGAGCCG CGGGGCCAGC AGGTCTTCCT CAAGCCGGAC GAGCCGCCGC 
     1141 CGCAGCCATG CGCCGACAGC CTGCAGGATG CTTTGCTGAG CCTAGGCGCC GTTATCGACA 
     1201 TTGCTGGCCT GCGACAGGCT GCCAGGGATG CCCTCTCAGC CGTGCTCCCC AAAGTGGAGA 
     1261 CTGTCTACAC CTACCTGCTA GATGGGGAGT CCAGACTGGT GTGTGAGGAC CCCCCTCATG 
     1321 AGCTGCCACA GGAAGGAAAA ATTCGAGAAG CTGTGATCTC TCAGAAGCGG CTGAGTTGCA 
     1381 ATGGGCTGGG ACCTTCGGAC CTACTGGGAA AGCCCTTGGC CAGGCTGGTG GCTCCACTGG 
     1441 CTCCTGACAT GCAAGTGCTG GTCATACCCC TGCTGGACAA GGAGACTGGA AGTGTGGCAG 
     1501 CTGTCATCTT GGTGCACTGT GGCCAGCTGA GTGACAGTGA GGAACAGAGC TTGCAGGTGG 
     1561 TAGAGAAGCA CGCTCTGGTA GCCCTGCGGA GGGTGCAGGC CCTGCAGCAG CGCAGGCCTG 
     1621 AAGCTGTTCA GAACACGTCA GTGGATGCCT CTGAAGATCA AAAGGATGAG AAGGGGTACA 
     1681 CCGACCATGA CCGAAAGATC CTGCAACTGT GTGGGGAACT CTTTGACTTG GATGCTACTT 
     1741 CTCTGCAGCT CAAAGTCCTT CAATATCTGC AGCAGGAGAC ACAGGCCACT CACTGCTGCC 
     1801 TCCTGCTGGT GTCGGAGGAC AATCTACAGC TTTCCTGCAA GGTCATTGGA GACAAAGTGC 
     1861 TGGGAGAAGA GGTCAGCTTT CCATTGACCA TGGGACGTCT GGGGCAGGTG GTGGAAGACA 
     1921 AGCAGTGTAT CCAGTTGAAG GACCTAACCT CTGACGATGT GCAACAGCTG CAAAACATGT 
     1981 TGGGTTGTGA GCTGCAGGCT ATGCTGTGTG TCCCTGTCAT CAGCCGAGCC ACTGACCAGG 
     2041 TGGTGGCCCT GGCTTGCGCC TTCAACAAGC TTGGAGGAGA TTTCTTCACA GATGAGGATG 
     2101 AACACGTGAT CCAACACTGC TTCCACTACA CAGGCACGGT GCTCACCAGC ACCTTGGCCT 
     2161 TCCAGAAGGA GCAGAAGCTC AAGTGTGAGT GCCAGGCTCT TCTCCAAGTG GCAAAGAACC 
     2221 TCTTCACCCA CCTGGATGAT GTCTCTGTCC TGCTACAGGA GATCATCACG GAAGCCAGAA 
     2281 ACCTCAGCAA CGCAGAGATC TGCTCGGTGT TCCTGCTGGA TCAGAACGAG CTGGTGGCCA 
     2341 AGGTGTTCGA TGGTGGCGTT GTGGACGATG AGAGTTATGA GATCCGCATC CCGGCGGACC 
     2401 AAGGCATCGC GGGCCACGTG GCGACTACGG GCCAGATCCT GAACATCCCA GATGCATATG 
     2461 CCCATCCGCT TTTCTATCGC GGCGTAGATG ACAGCACTGG CTTCCGCACG CGCAACATTC 
     2521 TCTGCTTCCC TATCAAGAAC GAGAACCAGG AGGTCATTGG TGTGGCTGAG CTAGTGAACA 
     2581 AGATCAATGG GCCATGGTTC AGCAAGTTTG ATGAGGACCT GGCCACAGCC TTCTCCATCT 
     2641 ACTGTGGCAT CAGCATCGCC CACTCTCTCC TATACAAAAA GGTGAATGAA GCCCAATACC 
     2701 GCAGCCACCT GGCCAATGAG ATGATGATGT ATCATATGAA GGTCTCTGAT GATGAATACA 
     2761 CCAAGCTTCT CCACGATGGC ATCCAACCTG TGGCCGCCAT TGACTCCAAC TTTGCCAACT 
     2821 TTACCTACAC GCCTCGGTCT CTGCCTGAGG ACGACACTTC TATGGCCATC CTGAGCATGC 
     2881 TGCAAGACAT GAACTTCATC AATAACTACA AAATTGACTG CCCAACTCTG GCCCGATTCT 
     2941 GCCTGATGGT GAAGAAAGGC TACCGGGATC CACCCTACCA CAACTGGATG CACGCCTTCT 
     3001 CTGTCTCTCA TTTTTGCTAC CTGCTCTACA AGAATCTGGA GCTCTCCAAC TACCTCGAGG 
     3061 ACATCGAGAT CTTTGCATTG TTTATTTCCT GCATGTGTCA TGACCTGGAC CACAGAGGCA 
     3121 CAAACAACTC CTTCCAGGTG GCCTCGAAAT CTGTGCTGGC CGCACTCTAC AGCTCAGAGG 
     3181 GCTCTGTCAT GGAGAGGCAC CACTTTGCTC AAGCCATTGC TATCCTCAAC ACCCACGGCT 
     3241 GCAATATCTT TGACCACTTC TCTCGGAAGG ACTATCAGCG CATGCTGGAC CTGATGAGGG 
     3301 ACATCATCTT GGCTACAGAC CTGGCACACC ACCTCCGCAT CTTCAAGGAC CTGCAGAAGA 
     3361 TGGCTGAAGT GGGTTATGAC CGAAACAACA GGCAACACCA CAGGCTTCTT CTGTGCCTCC 
     3421 TCATGACCTC CTGTGACCTC TCTGACCAGA CAAAGGGCTG GAAGACCACC AGAAAGATTG 
     3481 CAGAGCTGAT CTACAAAGAG TTCTTCTCCC AGGGAGACTT GGAGAAGGCC ATGGGCAACC 
     3541 GACCGATGGA GATGATGGAC CGTGAGAAGG CCTACATCCC TGAGCTTCAG ATCAGCTTTA 
     3601 TGGAGCACAT TGCCATGCCT ATCTACAAGC TTTTACAAGA CCTGTTCCCC AAGGCGGCAG 
     3661 AGCTGTATGA ACGTGTGGCC TCCAACCGTG AGCACTGGAC CAAGGTGTCC CACAAGTTCA 
     3721 CCATCCGAGG CCTCCCCAGT AACAACTCGC TGGATTTCCT GGACGAGGAA TACGAGGTCC 
     3781 CCGATTTGGA CGGCACCAGA GCTCCTGTCA ATGGCTGCTG CAGCCTCGAG GGCACGCGTA 
     3841 CGCGGCCGCT CGAGATGGAG AGCGACGAGA GCGGCCTGCC CGCCATGGAG ATCGAGTGCC 
     3901 GCATCACCGG CACCCTGAAC GGCGTGGAGT TCGAGCTGGT GGGCGGCGGA GAGGGCACCC 
     3961 CCGAGCAGGG CCGCATGACC AACAAGATGA AGAGCACCAA AGGCGCCCTG ACCTTCAGCC 
     4021 CCTACCTGCT GAGCCACGTG ATGGGCTACG GCTTCTACCA CTTCGGCACC TACCCCAGCG 
     4081 GCTACGAGAA CCCCTTCCTG CACGCCATCA ACAACGGCGG CTACACCAAC ACCCGCATCG 
     4141 AGAAGTACGA GGACGGCGGC GTGCTGCACG TGAGCTTCAG CTACCGCTAC GAGGCCGGCC 
     4201 GCGTGATCGG CGACTTCAAG GTGATGGGCA CCGGCTTCCC CGAGGACAGC GTGATCTTCA 
     4261 CCGACAAGAT CATCCGCAGC AACGCCACCG TGGAGCACCT GCACCCCATG GGCGATAACG 
     4321 ATCTGGATGG CAGCTTCACC CGCACCTTCA GCCTGCGCGA CGGCGGCTAC TACAGCTCCG 
     4381 TGGTGGACAG CCACATGCAC TTCAAGAGCG CCATCCACCC CAGCATCCTG CAGAACGGGG 
     4441 GCCCCATGTT CGCCTTCCGC CGCGTGGAGG AGGATCACAG CAACACCGAG CTGGGCATCG 
     4501 TGGAGTACCA GCACGCCTTC AAGACCCCGG ATGCAGATGC CGGTGAAGAA AGAGTTTAAA 
     4561 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4621 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4681 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4741 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4801 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4861 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4921 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4981 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5041 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5101 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5161 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5221 TTGGGTACGC GGCCAGCTTG GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA 
     5281 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 
     5341 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5401 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 
     5461 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG 
     5521 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 
     5581 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 
     5641 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 
     5701 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 
     5761 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 
     5821 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 
     5881 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 
     5941 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 
     6001 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 
     6061 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 
     6121 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 
     6181 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 
     6241 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC CGACCGCTAT 
     6301 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 
     6361 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 
     6421 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 
     6481 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 
     6541 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 
     6601 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 
     6661 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 
     6721 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 
     6781 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 
     6841 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 
     6901 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 
     6961 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 
     7021 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 
     7081 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 
     7141 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 
     7201 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 
     7261 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 
     7321 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 
     7381 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 
     7441 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 
     7501 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 
     7561 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 
     7621 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 
     7681 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC 
     7741 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 
     7801 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 
     7861 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 
     7921 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 
     7981 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 
     8041 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 
     8101 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 
     8161 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 
     8221 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 
     8281 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 
     8341 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 
     8401 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 
     8461 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 
     8521 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 
     8581 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 
     8641 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 
     8701 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 
     8761 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 
     8821 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 
     8881 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC 
     8941 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC 
     9001 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG 
     9061 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT 
     9121 TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC 
     9181 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT 
     9241 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA 
     9301 TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA 
     9361 ATTTT
//