LOCUS       MG211155                9293 bp    DNA     circular  SYN 
DEFINITION  Chaf1a (tGFP-tagged) - Mouse chromatin assembly factor 1, subunit A (p150) (Chaf1a)
ACCESSION   NM_013733
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3774..4478 
                     /label="GFP"  
     misc_feature    4525..5110 
                     /label="PolyA signal"  
     misc_feature    7202..7847 
                     /label="ColE1"  
     misc_feature    5564..6358 
                     /label="Neo"  
     misc_feature    5195..5497 
                     /label="SV40 ori"  
     misc_feature    7862..8722 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3753..3759 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3753 
                     /label="Chaf1a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGGAGGAG CCGGAGGCCG CGACGCGGAC AGCCGCGGCC GTGGATTGCA 
     1081 AAGACAGACC CGGGTTCCCA GTGAAGAGGT TAATACAAGC CCGCTTGCCC TTCAAACGCC 
     1141 TGAACCTTGT TCCCAAGGAG AAAGTTGAGG AAGACACATC ACCAAAAGCA GCTGTGGAAA 
     1201 GCAAAGTCCC GGATCTACAA TTGTCTTTGG GCACTTTTGA AAGCCAGTGT CACACAGGCT 
     1261 CTCACGTAGG CTTGAGTACA AAGTTGGTCG GTGGCCAGGG CCCCATTGAT AGCTTCTTGA 
     1321 GGGCAACAAT CAAGCCAGTA CCGAGTGTGG TCATTATCGA CCTGACGGAG AACTGCAGTG 
     1381 ACATTCCAGA CAGCCCTGAG GGCCACAGTG AACTAAGTCC TGACACTGCT GGTGTGGTCA 
     1441 CCACAGTGGA AGGAGCTGCC AAGCAGCAGG AGCACTCTGC AGCAGAGCTG TGTCTCCTAG 
     1501 AGACCCCTTC AGACATCACT TGTCACATGG AAGAGGAGCC TGGGAGTCCA GGAGACCCAA 
     1561 AGAGGACGGG TGACTGTCAG GCTGGTTCAC TACAGAGCTG TCCGGAGCTG ACCCCAGGTT 
     1621 CTAGGACATG CCCCACCAAG GAGTTATCAA GCTGGAGCAA GGCAGGGGAT CTCCTATTTA 
     1681 TAGAGAAAGT GCCTGTGGTG GTGCTAGAGG ACATCCTGGC CACTAAGCCC TCCATTGCCT 
     1741 CTCTTCCCAT GATGTCCCTG GATAGGAGTG TCACTTCAGA GAGTGAAATA CTGGAGTCCT 
     1801 GCCCCGAGGA TGACTCCATA CTGAGCCACT CCTCCACCAA CTCCTCTTCT CCAACCAGCT 
     1861 CTCCTGAGGG ACCATCCACA CCCCCAGAAC ATCGGGGTGG GAGAAGCTCC CCCTCCACCC 
     1921 CAGCCTGCAG AGTTGCCAAG AACTTTGTCA AAGGCTCCAC AGAGAAGGGC AGGAGCAAAC 
     1981 TGCACAGAGA TAGAGAACAG CAGAGGGAAG AGAAGGAAAA GCTGAGGGAA GAGATACGAC 
     2041 GAGCCAAGGA GGAGGCACGG AAGAAGAAGG AGGAGGAGAA GGAGCTGAAG GAAAAGGAGC 
     2101 GGAGGGAAAA GCGGGAGAAA GATGAGAAGG AGAAGGCGGA GAAGCAGCGG CTAAAGGAGG 
     2161 AAAAACGCAA GGAGCGGCAG GAAGCTCTGG AGGCTAAACT GGAGGAGAAG AGGAAGAAGG 
     2221 AGGAAGAGAA GCGGTTACGG GAGGAAGAGA AGCGGTTACG GGAAGAAGAG AAGCGCATTA 
     2281 AGGCAGAAAA GGCAGAGATC ACTCGATTCT TCCAGAAACC GAAGACTCCG CAGGCTCCCA 
     2341 AGACCCTGGC CGGTTCCTGT GGTAAGTTTG CCCCCTTTGA AATCAAAGAA CACATGGTGC 
     2401 TGGCTCCACG GTGCCGAGCT GCCTTGGATC AAGACCTGTG CGATCAGCTG GACCAGCTCC 
     2461 TGCAGCAGCA GAGTGTGGCA AGCACCTTCC TCAGTGACCT AAAAAGCCGG CTGCCCTTGA 
     2521 GATCGGGGCC CACCCGGGTC TGTGGCCATG ACACAGACAT CATGAACAGG GATGTGGTCA 
     2581 TTGTGGAGAG CAGCAAGGTG GACGGTGTGT CTGAAAGGAA GAAGTTTGGC CGCATGAAGC 
     2641 TTCTGCAGTT CTCAGAGAAC CACCGGCCAG CATACTGGGG GACGTGGAAC AAGAAGACAG 
     2701 CCATCATCCG CCCCCGGAAC CCCTGGGCCC AGGACAAAGA CCTTCTTGAC TATGAGGTAG 
     2761 ACAGTGACGA TGAGTGGGAA GAGGAGGAAC CTGGGGAGTC TTTGTCCCAC AGTGAGGGGG 
     2821 ATGAGGATGA CGATGTGGGG GAAGATGAGG ATGAGGACGA TGGCTTCTTT GTGCCCCATG 
     2881 GGTACTTATC TGAAGATGAA GGCGTGACTG AGGAGTGTGC AGACCCTGAG AACCACAAAG 
     2941 TACACCAGAA GCTGAAGGCC AAGGAGTGGG ATGAGCTCCT AGCCAAGGGC AAGCGCTTTC 
     3001 GTGTGCTGCA GCCTGTGCAC GTGGGATGTG TATGGGCATC AGAGGCAGCC AACTGCACGA 
     3061 GTTCCGACTT GAAGCTGCTG CAGCAGTTCA CTGCATGCCT GCTGGATGTG GCATCCCCTG 
     3121 ATGAGCCAGA ACCCGGGGCC TCCAGGAGGG AAAAGAGGGA CCAGCATATC CTGGCCCAGT 
     3181 TGCTTCCGCT GCTACATGGC AATGTGAATG GGAGCAAGGT GATCATCCAT GAATTCCAGG 
     3241 AGCAATGCCG CAGGGGCCTG CTGACCCTTC CAAGTCCCAC CCCACACCTG CAGATGCCCA 
     3301 ACTTGGAGGA TGCTGTTGCT GTGCCATCCA AGGCCAGGCT GAAGCGCCTT ATCTCTGAGA 
     3361 ACTCTGCCTA TGAGAAGCGG CCCAACTTCC GTATGTGCTG GTATGTGCAC CCAGAGGTGC 
     3421 TCAAGAGCTT TGGCCAGGAG TGCCTGCCAG TGCCCTGCCA GTGGACCTAC ATCACCACCA 
     3481 TGCCCTCAGC ACCCAGGGAA GACAGTGGCA GTGCTTCTAC TGAGGGGCCT GGCCAGAGCA 
     3541 CACCTATGCT ACTCAAGCGG AAGCCAGCGG CCACCATGTG CATCACGCAG TTTATGAAGA 
     3601 AGCGGCGCTA TGATGGACAG GTTGGGTCTG GGGACATGGA TGGCTTCCAG GCAGACACAG 
     3661 AAGAGGATGA AGAGGATGAC ACAGACTGTA TGATCATAGA TGTGCCAGAT GTTGGGAGTG 
     3721 ACGTGTCAGA AGCTCCCATA CCTGCTCCCA CACTTTGCAA GACGCGTACG CGGCCGCTCG 
     3781 AGATGGAGAG CGACGAGAGC GGCCTGCCCG CCATGGAGAT CGAGTGCCGC ATCACCGGCA 
     3841 CCCTGAACGG CGTGGAGTTC GAGCTGGTGG GCGGCGGAGA GGGCACCCCC GAGCAGGGCC 
     3901 GCATGACCAA CAAGATGAAG AGCACCAAAG GCGCCCTGAC CTTCAGCCCC TACCTGCTGA 
     3961 GCCACGTGAT GGGCTACGGC TTCTACCACT TCGGCACCTA CCCCAGCGGC TACGAGAACC 
     4021 CCTTCCTGCA CGCCATCAAC AACGGCGGCT ACACCAACAC CCGCATCGAG AAGTACGAGG 
     4081 ACGGCGGCGT GCTGCACGTG AGCTTCAGCT ACCGCTACGA GGCCGGCCGC GTGATCGGCG 
     4141 ACTTCAAGGT GATGGGCACC GGCTTCCCCG AGGACAGCGT GATCTTCACC GACAAGATCA 
     4201 TCCGCAGCAA CGCCACCGTG GAGCACCTGC ACCCCATGGG CGATAACGAT CTGGATGGCA 
     4261 GCTTCACCCG CACCTTCAGC CTGCGCGACG GCGGCTACTA CAGCTCCGTG GTGGACAGCC 
     4321 ACATGCACTT CAAGAGCGCC ATCCACCCCA GCATCCTGCA GAACGGGGGC CCCATGTTCG 
     4381 CCTTCCGCCG CGTGGAGGAG GATCACAGCA ACACCGAGCT GGGCATCGTG GAGTACCAGC 
     4441 ACGCCTTCAA GACCCCGGAT GCAGATGCCG GTGAAGAAAG AGTTTAAACG GCCGGCCGCG 
     4501 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4561 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4621 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4681 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4741 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4801 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4861 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4921 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4981 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5041 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5101 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5161 CCAGCTTGGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     5221 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA 
     5281 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC 
     5341 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC 
     5401 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA 
     5461 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA 
     5521 GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT 
     5581 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT 
     5641 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG 
     5701 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC 
     5761 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC 
     5821 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC 
     5881 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG 
     5941 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG 
     6001 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT 
     6061 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG 
     6121 GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC 
     6181 TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCCG ACCGCTATCA GGACATAGCG 
     6241 TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG 
     6301 CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG 
     6361 TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT 
     6421 CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC 
     6481 GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC 
     6541 CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA 
     6601 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT 
     6661 CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC 
     6721 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA 
     6781 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT 
     6841 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC 
     6901 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC 
     6961 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 
     7021 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 
     7081 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 
     7141 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 
     7201 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 
     7261 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 
     7321 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 
     7381 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 
     7441 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 
     7501 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG 
     7561 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 
     7621 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 
     7681 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 
     7741 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 
     7801 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 
     7861 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC 
     7921 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC 
     7981 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT 
     8041 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG 
     8101 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA 
     8161 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA 
     8221 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT 
     8281 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG 
     8341 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA 
     8401 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC 
     8461 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT 
     8521 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC 
     8581 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA 
     8641 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA 
     8701 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA 
     8761 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 
     8821 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG 
     8881 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC 
     8941 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC 
     9001 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG 
     9061 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG 
     9121 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG 
     9181 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT 
     9241 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//