LOCUS       MG211123                9272 bp    DNA     circular  SYN 
DEFINITION  Mcm2 (tGFP-tagged) - Mouse minichromosome maintenance deficient 2 mitotin (S
ACCESSION   NM_008564
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3753..4457 
                     /label="GFP"  
     misc_feature    4504..5089 
                     /label="PolyA signal"  
     misc_feature    7181..7826 
                     /label="ColE1"  
     misc_feature    5543..6337 
                     /label="Neo"  
     misc_feature    5174..5476 
                     /label="SV40 ori"  
     misc_feature    7841..8701 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3732..3738 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3732 
                     /label="Mcm2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGTCT TCTGAGTCTC TCTCAGCATC TAGCCCTGCC CGTCAGCGGC 
     1081 GCCGGATCAG TGATCCCCTC ACCTCCAGCC CAGGCCGCAG CTCCAGACGT GCTGACGCCC 
     1141 TGACCTCCAG CCCTGGCAGA GACCTCCCCC CATTTGAAGA TGAGTCTGAG GGGCTGCTGG 
     1201 GCACAGAGGG GCCCATGGAG GAAGAAGAGG ATGGAGAGGA ACTCATTGGT GATGGCATGG 
     1261 AGAGAGACTA CCGTCCCATT CCGGAGCTCG ATGTCTACGA GGCCGAGGGA TTGGCCCTGG 
     1321 ATGATGAAGA TGTGGAGGAG CTGACAGCCA GTCAGAGAGA GGCAGCTGAG CGGACCATGA 
     1381 GGCAGCGGGA CCGTGAGGCT GGCAGAGGCC TGGGACGCAT GCGCCGGGGG CTGCTCTATG 
     1441 ACAGCAGCGA GGAAGATGAG GAGCGGCCTG CCCGTAAGCG CCGCCACGTA GAACGCGCCA 
     1501 CAGAGGATGG CGAGGAGGAT GAAGAGATGA TCGAGAGTAT TGAGAATCTG GAGGACCTCA 
     1561 AGGGCCACTC GGTGCGCGAG TGGGTGAGCA TGGCAGGGCC CAGGCTGGAG ATCCACCACC 
     1621 GCTTCAAGAA CTTCCTGCGC ACCCACGTGG ACAGCCATGG CCACAACGTC TTCAAGGAGC 
     1681 GCATCAGTGA TATGTGCAAA GAGAACCGTG AGAGTTTGGT GGTAAATTAT GAAGACCTGG 
     1741 CAGCCCGGGA GCACGTGTTG GCATACTTCC TGCCGGAAGC ACCGGCTGAG TTGCTGCAGA 
     1801 TCTTTGACGA GGCTGCCCTG GAGGTCGTGT TGGCCATGTA CCCTAAATAT GACCGTATCA 
     1861 CCAACCACAT CCATGTGCGC ATCTCCCACC TGCCTCTGGT GGAGGAGCTG CGTTCACTGA 
     1921 GGCAGTTGCA CCTGAACCAG CTGATCCGTA CCAGTGGCGT GGTGACCAGC TGCACCGGAG 
     1981 TCCTGCCCCA GCTCAGCATG GTCAAGTACA ACTGTAGCAA GTGCAACTTT GTACTGGGGC 
     2041 CTTTCTGCCA GTCTCAGAAT CAGGAGGTGA AGCCTGGCTC CTGCCCTGAG TGCCAGTCTG 
     2101 CTGGGCCCTT TGAGATCAAC ATGGAGGAGA CCATCTATCA GAACTACCAA CGTATCCGCA 
     2161 TCCAGGAGAG TCCCGGCAAG GTGGCGGCTG GCCGACTGCC CCGTTCCAAG GATGCCATTC 
     2221 TCCTCGCTGA TCTGGTGGAC AGCTGCAAGC CAGGGGACGA GATAGAGCTG ACCGGCATTT 
     2281 ACCATAATAA CTATGACGGC TCGCTTAACA CCGCCAACGG CTTTCCAGTC TTTGCCACTA 
     2341 TTATCTTGGC CAACCATGTT GCCAAGAAGG ACAACAAAGT AGCTGTGGGG GAGCTCACCG 
     2401 ATGAGGACGT GAAGATGATC ACCGGTCTCT CCAAGGATCA GCAAATTGGA GAGAAGATCT 
     2461 TTGCCAGCAT TGCACCCTCC ATCTATGGGC ATGAAGACAT CAAGAGAGGC CTGGCTCTGG 
     2521 CCCTGTTTGG AGGGGAGCCC AAGAACCCAG GTGGAAAGCA CAAGGTTCGA GGTGACATTA 
     2581 ATGTGCTCTT GTGTGGGGAC CCTGGCACAG CAAAGTCCCA ATTCCTCAAA TACATCGAGA 
     2641 AAGTGTCTAG CCGTGCCATC TTCACCACTG GCCAGGGTGC GTCAGCAGTG GGTCTCACCG 
     2701 CGTACGTTCA GCGGCATCCC GTCAGCAGAG AGTGGACCTT AGAGGCGGGA GCCCTGGTTC 
     2761 TGGCTGACCG GGGGGTGTGT CTCATTGACG AGTTTGACAA GATGAATGAC CAGGACAGGA 
     2821 CCAGCATCCA CGAGGCCATG GAACAGCAAA GCATCTCCAT CTCCAAGGCT GGCATCGTTA 
     2881 CCTCGCTGCA AGCCCGCTGC ACTGTCATAG CTGCTGCCAA CCCCATAGGA GGCCGCTACG 
     2941 ACCCTTCACT GACCTTCTCA GAGAATGTAG ACCTCACAGA GCCCATCATT TCCCGCTTTG 
     3001 ATGTCCTGTG TGTGGTGAGG GACACTGTTG ATCCAGTTCA GGATGAGATG CTGGCCCGCT 
     3061 TTGTGGTTGG CAGCCACGTC AGACACCACC CCAGTAACAA GAAGGATGAA GGGTTGACTA 
     3121 ATGGTGGCAC CTTGGAGCCA GCCATGCCCA ACACATATGG CGTGGAGCCC CTGCCTCAGG 
     3181 AGGTGCTGAA GAAGTATATC ATCTATGCCA AGGAGAGGGT CCGCCCGAAG CTCAACCAGA 
     3241 TGGACCAGGA TAAAGTGGCC AGGATGTACA GTGACCTGAG GAAGGAGTCC ATGGCAACGG 
     3301 GCAGCATTCC CATCACGGTG CGCCACATCG AGTCCATGAT CCGCATGGCC GAGGCCCATG 
     3361 CCCGCATGCA CCTGCGGGAC TACGTGATGG AAGACGATGT CAACATGGCC ATCCGAGTGA 
     3421 TGATGGAGAG CTTCATTGAC ACCCAGAAGT TCAGCGTCAT GCGGAGTATG CGCAAGACTT 
     3481 TTGCCCGGTA TCTCTCCTTC CGGCGAGATA ACAATGATCT GCTGCTCTTC ATACTGAAGC 
     3541 AGTTGGTGGC TGAGCAGGTG ACATATCAAC GCAACCGCTT TGGGGCCCAG CAGGACACCA 
     3601 TTGAAATACC TGAGAAGGAT CTGATGGACA AGGCCAGGCA GATCAATATT CACAACCTCT 
     3661 CTGCCTTCTA CGACAGCGAC CTCTTCAAAT TCAACAAGTT CAGCCGTGAC CTGAAACGCA 
     3721 AACTGATCCT ACAGCAGTTC ACGCGTACGC GGCCGCTCGA GATGGAGAGC GACGAGAGCG 
     3781 GCCTGCCCGC CATGGAGATC GAGTGCCGCA TCACCGGCAC CCTGAACGGC GTGGAGTTCG 
     3841 AGCTGGTGGG CGGCGGAGAG GGCACCCCCG AGCAGGGCCG CATGACCAAC AAGATGAAGA 
     3901 GCACCAAAGG CGCCCTGACC TTCAGCCCCT ACCTGCTGAG CCACGTGATG GGCTACGGCT 
     3961 TCTACCACTT CGGCACCTAC CCCAGCGGCT ACGAGAACCC CTTCCTGCAC GCCATCAACA 
     4021 ACGGCGGCTA CACCAACACC CGCATCGAGA AGTACGAGGA CGGCGGCGTG CTGCACGTGA 
     4081 GCTTCAGCTA CCGCTACGAG GCCGGCCGCG TGATCGGCGA CTTCAAGGTG ATGGGCACCG 
     4141 GCTTCCCCGA GGACAGCGTG ATCTTCACCG ACAAGATCAT CCGCAGCAAC GCCACCGTGG 
     4201 AGCACCTGCA CCCCATGGGC GATAACGATC TGGATGGCAG CTTCACCCGC ACCTTCAGCC 
     4261 TGCGCGACGG CGGCTACTAC AGCTCCGTGG TGGACAGCCA CATGCACTTC AAGAGCGCCA 
     4321 TCCACCCCAG CATCCTGCAG AACGGGGGCC CCATGTTCGC CTTCCGCCGC GTGGAGGAGG 
     4381 ATCACAGCAA CACCGAGCTG GGCATCGTGG AGTACCAGCA CGCCTTCAAG ACCCCGGATG 
     4441 CAGATGCCGG TGAAGAAAGA GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4501 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4561 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4621 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4681 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4741 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4801 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4861 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4921 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4981 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5041 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5101 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCTTGGCT GTGGAATGTG 
     5161 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5221 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5281 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 
     5341 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 
     5401 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 
     5461 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 
     5521 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 
     5581 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 
     5641 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 
     5701 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 
     5761 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 
     5821 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 
     5881 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 
     5941 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 
     6001 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 
     6061 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 
     6121 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 
     6181 GGCCGGCTGG GTGTGGCCGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 
     6241 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 
     6301 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 
     6361 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 
     6421 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 
     6481 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 
     6541 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 
     6601 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 
     6661 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 
     6721 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 
     6781 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 
     6841 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 
     6901 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 
     6961 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     7021 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     7081 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     7141 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     7201 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     7261 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     7321 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     7381 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     7441 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     7501 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     7561 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     7621 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 
     7681 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 
     7741 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 
     7801 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 
     7861 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 
     7921 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 
     7981 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 
     8041 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 
     8101 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 
     8161 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 
     8221 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 
     8281 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 
     8341 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 
     8401 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 
     8461 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 
     8521 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 
     8581 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 
     8641 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 
     8701 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
     8761 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
     8821 TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 
     8881 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 
     8941 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 
     9001 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 
     9061 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG 
     9121 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT 
     9181 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG 
     9241 AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//