LOCUS       MG211074                9236 bp    DNA     circular  SYN 
DEFINITION  Aco1 (tGFP-tagged) - Mouse aconitase 1 (Aco1)
ACCESSION   NM_007386
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3715..4419 
                     /label="GFP"  
     misc_feature    4466..5051 
                     /label="PolyA signal"  
     misc_feature    7143..7788 
                     /label="ColE1"  
     misc_feature    5505..6299 
                     /label="Neo"  
     misc_feature    5136..5438 
                     /label="SV40 ori"  
     misc_feature    7803..8663 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3687..3694 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3687 
                     /label="Aco1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGAACCCA TTTGCACACC TTGCTGAGCC CTTGGACGCT GCCCAACCAG 
     1081 GAAAGAGGTT CTTCAACTTG AATAAATTGG AGGACTCAAG ATACGGACGC TTACCATTTT 
     1141 CTATCAGAGT TCTCCTGGAG GCAGCCGTTC GGAACTGTGA TGAGTTTCTG GTGAAGAAAA 
     1201 ATGACATCGA GAATATCCTG AATTGGAATG TCATGCAACA TAAGAACATA GAAGTGCCGT 
     1261 TTAAGCCAGC CCGAGTCATA CTGCAAGACT TTACGGGTGT GCCTGCTGTG GTGGATTTTG 
     1321 CAGCAATGCG CGATGCTGTG AAGAAGTTGG GAGGGAATCC GGAGAAAATT AACCCTGTCT 
     1381 GCCCCGCTGA CCTTGTAATT GATCATTCCA TCCAGGTTGA TTTCAACAGA AGGGCAGACA 
     1441 GTTTACAGAA GAATCAAGAC CTGGAGTTTG AAAGAAATAA AGAACGATTT GAATTTCTAA 
     1501 AGTGGGGTTC CCAGGCCTTT TGCAACATGC GGATAATCCC TCCTGGCTCG GGAATCATTC 
     1561 ACCAAGTGAA CCTGGAGTAT TTGGCAAGAG TAGTGTTTGA TCAGGATGGA TGCTACTACC 
     1621 CAGACAGCCT CGTGGGCACA GATTCACACA CGACCATGAT TGACGGCCTG GGAGTTCTTG 
     1681 GTTGGGGTGT GGGTGGTATT GAAGCAGAAG CTGTCATGCT GGGTCAGCCC ATCAGCATGG 
     1741 TGCTTCCCCA GGTGATTGGC TACAAGCTGA TGGGGAAGCC TCACCCTCTG GTAACATCCA 
     1801 CGGACATCGT GCTCACCATT ACCAAGCACC TCCGACAAGT GGGAGTTGTG GGCAAATTTG 
     1861 TGGAGTTTTT CGGGCCAGGA GTGGCTCAGC TGTCCATTGC TGACCGAGCT ACGATTGCCA 
     1921 ATATGTGCCC AGAGTATGGT GCGACAGCAG CCTTCTTCCC GGTTGATGAA GTTAGCATCG 
     1981 CGTACCTGCT GCAGACAGGC CGCGAGGAAG ACAAAGTCAA GCACATTCAG AAGTATCTTC 
     2041 AGGCTGTAGG CATGTTTCGA GATTTCAACG ACACCTCTCA AGACCCAGAC TTCACTCAGG 
     2101 TTGTGGAGTT AGATCTGAAA ACAGTTGTGC CTTGCTGCAG TGGACCCAAA AGACCTCAGG 
     2161 ACAAAGTCGC AGTGTCTGAG ATGAAAAAGG ACTTTGAAAG CTGCCTTGGA GCCAAGCAAG 
     2221 GATTTAAAGG TTTCCAAGTT GCTTCAGACC GTCACAATGA CCGCAAGACG TTCCTCTATA 
     2281 ATAACAGTGA ATTCACTCTC GCTCACGGCT CTGTGGTAAT CGCTGCCATC ACTAGCTGCA 
     2341 CAAACACCAG CAATCCATCC GTGATGTTAG GAGCAGGATT GTTAGCAAAG AAAGCTGTGG 
     2401 AGGCGGGGCT GAGTGTGAAG CCTTACATCA AAACCAGCCT GTCTCCTGGA AGTGGAGTGG 
     2461 TCACCTACTA CCTTCGAGAG AGTGGAGTCA TGCCTTACCT GTCCCAGTTA GGGTTTGATG 
     2521 TGGTGGGCTA CGGCTGCATG ACCTGCATCG GCAACAGTGG ACCCCTTCCT GAACCTGTGG 
     2581 TCGAGGCGAT CACCCAGGGA GACCTCGTAG CTGTTGGCGT TCTGTCTGGG AATAGGAATT 
     2641 TTGAAGGCCG AGTCCATCCT AACACACGAG CCAACTACTT AGCATCCCCC CCTCTGGTAA 
     2701 TAGCATATGC GATTGCAGGC ACCGTCAGGA TTGACTTCGA GAAAGAGCCT TTGGGAGTGA 
     2761 ACGCACAGGG CCGGCAAGTG TTTCTGAAGG ATATCTGGCC CACGCGAGAT GAGATCCAGG 
     2821 CGGTGGAAAG GCAGTATGTC ATCCCCGGGA TGTTTAAGGA GGTCTATCAG AAAATAGAGA 
     2881 CTGTAAATAA AAGCTGGAAT GCCTTAGCAG CCCCGTCAGA GAAGCTGTAC GCGTGGAATC 
     2941 CCAAATCTAC TTACATTAAG TCGCCGCCAT TCTTTGAAAG CTTGACTTTA GATCTCCAGC 
     3001 CACCCAAGTC CATAGTGGAT GCCTATGTGC TACTGAATTT AGGAGATTCA GTAACAACGG 
     3061 ACCATATCTC TCCCGCCGGC AATATTGCAA GAAACAGCCC TGCGGCTCGC TACTTGACGA 
     3121 ACAGAGGCCT AACACCACGA GAGTTCAACT CCTATGGCTC CCGCCGGGGT AACGACGCCA 
     3181 TCATGGCCCG GGGGACATTT GCCAACATTC GCTTGCTGAA CAAGTTTCTG AACAAGCAGG 
     3241 CACCTCAGAC TGTCCATCTC CCTTCAGGAG AAACCCTTGA TGTATTCGAC GCTGCTGAGC 
     3301 GGTACCAGCA GGCTGGACTT CCCCTGATTG TTCTGGCTGG GAAAGAATAC GGTTCAGGCA 
     3361 GCTCCCGAGA CTGGGCAGCC AAAGGCCCTT TCCTGCTGGG AATCAAAGCT GTCCTGGCAG 
     3421 AGAGCTATGA GCGCATTCAC CGCAGCAACT TGGTTGGCAT GGGGGTGATC CCCCTTGAGT 
     3481 ATCTCCCTGG TGAAACTGCA GACTCTCTGG GACTCACAGG TCGGGAAAGA TACACTATCA 
     3541 ACATCCCTGA AGACCTCAAG CCTCGCATGA CGGTGCAGAT CAAGCTGGAC ACTGGGAAGA 
     3601 CCTTCCAGGC CGTGATGAGG TTCGACACTG ATGTGGAGCT CACATACTTC CACAATGGAG 
     3661 GCATCCTGAA CTACATGATC CGAAAGATGG CCCAGAGCGG ACCGACGCGT ACGCGGCCGC 
     3721 TCGAGATGGA GAGCGACGAG AGCGGCCTGC CCGCCATGGA GATCGAGTGC CGCATCACCG 
     3781 GCACCCTGAA CGGCGTGGAG TTCGAGCTGG TGGGCGGCGG AGAGGGCACC CCCGAGCAGG 
     3841 GCCGCATGAC CAACAAGATG AAGAGCACCA AAGGCGCCCT GACCTTCAGC CCCTACCTGC 
     3901 TGAGCCACGT GATGGGCTAC GGCTTCTACC ACTTCGGCAC CTACCCCAGC GGCTACGAGA 
     3961 ACCCCTTCCT GCACGCCATC AACAACGGCG GCTACACCAA CACCCGCATC GAGAAGTACG 
     4021 AGGACGGCGG CGTGCTGCAC GTGAGCTTCA GCTACCGCTA CGAGGCCGGC CGCGTGATCG 
     4081 GCGACTTCAA GGTGATGGGC ACCGGCTTCC CCGAGGACAG CGTGATCTTC ACCGACAAGA 
     4141 TCATCCGCAG CAACGCCACC GTGGAGCACC TGCACCCCAT GGGCGATAAC GATCTGGATG 
     4201 GCAGCTTCAC CCGCACCTTC AGCCTGCGCG ACGGCGGCTA CTACAGCTCC GTGGTGGACA 
     4261 GCCACATGCA CTTCAAGAGC GCCATCCACC CCAGCATCCT GCAGAACGGG GGCCCCATGT 
     4321 TCGCCTTCCG CCGCGTGGAG GAGGATCACA GCAACACCGA GCTGGGCATC GTGGAGTACC 
     4381 AGCACGCCTT CAAGACCCCG GATGCAGATG CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC 
     4441 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4501 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4561 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4621 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4681 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4741 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4801 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4861 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4921 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4981 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5041 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5101 CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5161 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC 
     5221 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA 
     5281 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG 
     5341 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG 
     5401 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG 
     5461 GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 
     5521 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 
     5581 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 
     5641 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 
     5701 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 
     5761 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 
     5821 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 
     5881 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 
     5941 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 
     6001 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC 
     6061 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 
     6121 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CCGACCGCTA TCAGGACATA 
     6181 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 
     6241 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 
     6301 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC 
     6361 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 
     6421 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 
     6481 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 
     6541 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 
     6601 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT 
     6661 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 
     6721 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 
     6781 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 
     6841 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 
     6901 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 
     6961 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 
     7021 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 
     7081 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 
     7141 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 
     7201 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 
     7261 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 
     7321 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 
     7381 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 
     7441 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA 
     7501 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 
     7561 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA 
     7621 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 
     7681 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 
     7741 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 
     7801 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 
     7861 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 
     7921 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 
     7981 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 
     8041 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 
     8101 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 
     8161 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 
     8221 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 
     8281 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 
     8341 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 
     8401 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 
     8461 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 
     8521 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 
     8581 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 
     8641 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 
     8701 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 
     8761 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG 
     8821 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 
     8881 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 
     8941 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 
     9001 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 
     9061 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 
     9121 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA 
     9181 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//