LOCUS       MG211068                9221 bp    DNA     circular  SYN 
DEFINITION  Kif20a (tGFP-tagged) - Mouse kinesin family member 20A (Kif20a)
ACCESSION   NM_009004
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3702..4406 
                     /label="GFP"  
     misc_feature    4453..5038 
                     /label="PolyA signal"  
     misc_feature    7130..7775 
                     /label="ColE1"  
     misc_feature    5492..6286 
                     /label="Neo"  
     misc_feature    5123..5425 
                     /label="SV40 ori"  
     misc_feature    7790..8650 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3681..3687 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3681 
                     /label="Kif20a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTCACCGG ATCCTTTCTC CGCCAGCGGG CTTACTCTCT GATGAGGATG 
     1081 TTGTAGACTC TCCCATATTA GAATCCACAG CTGCAGATCT GAGATCTGTT GTCCGAAAGG 
     1141 ACCTGTTGTC AGACTGCTCT GTCATCTCTG CCTCTCTGGA GGACAAGCAG GCTCTACTGG 
     1201 AGGACACCTC AGAGAAGGTG AAAGTTTACC TTCGAATTCG ACCATTCTTG ACTTCAGAGT 
     1261 TGGACCGACA GGAAGATCAG GGCTGCGTCT GCATTGAGAA TACAGAAACC CTTGTGCTGC 
     1321 AGGCACCCAA AGACTCCTTT GCCTTGAAGA GTAATGAGAG GGGAGTTGGT CAGGCCACTC 
     1381 ACAAATTTAC CTTTTCCCAG ATATTTGGGC CAGAAGTGGG ACAGGTGGCC TTCTTCAATC 
     1441 TGACCATGAA GGAGATGGTG AAGGATGTGC TCAAAGGGCA GAACTGGCTC ATCTACACCT 
     1501 ATGGAGTCAC CAACTCGGGG AAAACGTACA CCATTCAAGG TACTAGTAAG GATGCGGGGA 
     1561 TCCTGCCCCA GTCCCTGGCT CTCATCTTTA ATAGTCTCCA GGGCCAGCTT CATCCGACAC 
     1621 CTGACCTGAA GCCCTTATTG TCCAATGAAG TAATCTGGCT AGACAGCAAG CAGATTCGAC 
     1681 AGGAGGAAAT GAAGAAGCTG TCCTTGCTAA TTGGAGGCCT CCAAGAGGAG GAGCTGTCCA 
     1741 CCTCCGTGAA GAAACGTGTC CACACTGAAA GTCGGATAGG TGCCAGCAAC AGCTTTGACA 
     1801 GTGGCGTTGC TGGGCTGTCT TCCACCAGTC AGTTCACCAG CAGCAGCCAG CTAGATGAAA 
     1861 CAAGTCAATT ATGGGCACAA CCAGACACTG TCCCAGTAAG TGTTCCAGCA GACATTCGCT 
     1921 TCTCTGTCTG GATCTCCTTC TTTGAGATCT ACAATGAATT GCTTTATGAC CTGTTAGAAC 
     1981 CACCTAGCCA TCAGCATAAG AGACAGACAC TTCGGCTGTG TGAGGATCAG AACGGCAATC 
     2041 CTTACGTGAA AGATCTCAAT TGGATTCATG TTCGTGATGT TGAGGAAGCC TGGAAACTAT 
     2101 TGAAAGTGGG TCGCAAGAAC CAGAGCTTTG CCAGCACCCA CATGAACCAG CAATCCAGCC 
     2161 GCAGTCACAG CATCTTCTCA ATCAGAATCT TGCACCTTCA GGGAGAAGGG GATATAGTGC 
     2221 CCAAGATCAG TGAACTGTCA CTCTGTGACC TGGCTGGCTC TGAGCGCTGC AAACATCAAA 
     2281 AAAGTGGTGA GCGGCTAAAG GAGGCAGGGA ACATTAACAC TTCTCTGCAC ACCCTGGGCC 
     2341 GCTGTATTGC TGCCCTGCGA CAGAATCAGC AGAACCGGTC AAAGCAGAAC CTGATTCCTT 
     2401 TCCGTGACAG CAAGTTGACT CGTGTGTTCC AAGGCTTCTT CACAGGTCGA GGTCGTTCCT 
     2461 GTATGATTGT CAATGTGAAT CCCTGTGCAT CTACATATGA CGAGACTCTT CATGCAGCCA 
     2521 AATTCTCAGC CCTAGCCAGC CAGCTTGTGC ACGCCCCACC TGTGCATCTG GGAATCCCAT 
     2581 CCCTGCATTC ATTCATCAAG AAGCACAGTC CTCAGGTTGG CCCTGGCTTA GAGAAAGAGG 
     2641 ACAAGGCCGA CTCAGACCTT GAGGACAGTC CTGAAGACGA AGCTGACGTC TCCGTGTATG 
     2701 GCAAGGAGGA ACTACTCCAG GTGGTGGAAG CCATGAAAGC GCTACTTTTA AAAGAACGAC 
     2761 AAGAAAAGCT GCAGTTGGAG ATACAGCTCC GAGAGGAAAT TTGCAACGAA ATGGTGGAAC 
     2821 AGATGCAACA GCGGGAGCAG TGGTGCAGTG AGCGTTTGGA CAACCAAAAG GAACTGATGG 
     2881 AGGAACTGTA CGAGGAGAAA CTGAAGATCC TGAAGGAATC ACTGACAACT TTCTACCAAG 
     2941 AGCAGATCCA GGAGCGGGAT GAAAAAATTG AAGAGCTAGA AACTCTGTTG CAGGAAGCCA 
     3001 AACAGCAGCC AGCGGCACAA CAGTCAGGGG GGCTGTCCCT CCTCCGGCGG TCACAGAGGC 
     3061 TGGCAGCGTC TGCCTCCACT CAGCAGTTCC AGGAAGTTAA AGCTGAACTA GAGCAATGCA 
     3121 AAACAGAGTT AAGCTCTACC ACTGCAGAGC TGCACAAGTA TCAGCAAGTG TTAAAACCGC 
     3181 CACCCCCAGC CAAGCCTTTC ACCATTGATG TGGATAAGAA GTTAGAGGAG GGCCAGAAGA 
     3241 ATATAAGACT GCTACGGACA GAGCTCCAGA AACTAGGACA GTCTCTGCAG TCAGCAGAAA 
     3301 GAGCCTGTTG CCACAGCACC GGAGCAGGAA AACTTCGTCA AGCATTAACC AACTGTGATG 
     3361 ACATCTTAAT CAAGCAGAAC CAGACCCTGG CTGAGCTGCA GAATAACATG GTGCTAGTGA 
     3421 AACTGGACCT TCAGAAAAAG GCAGCCTGCA TTGCTGAGCA GTATCACACT GTGCTAAAGC 
     3481 TCCAAGGCCA AGCCTCTGCC AAAAAGCGGC TGGGAGCAAA CCAGGAAAAC CAGCAACCAA 
     3541 ATCATCAACC CCCAGGAAAG AAACCATTCC TGCGAAACTT ACTTCCCCGA ACACCTACCT 
     3601 GCCAAAGCTC AACAGACAGC AGCCCTTATG CGCGGATCTT GCGCTCACGG CACTCTCCTT 
     3661 TACTCAAATC TCCCTTTGGC AAAAAATACA CGCGTACGCG GCCGCTCGAG ATGGAGAGCG 
     3721 ACGAGAGCGG CCTGCCCGCC ATGGAGATCG AGTGCCGCAT CACCGGCACC CTGAACGGCG 
     3781 TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG GCACCCCCGA GCAGGGCCGC ATGACCAACA 
     3841 AGATGAAGAG CACCAAAGGC GCCCTGACCT TCAGCCCCTA CCTGCTGAGC CACGTGATGG 
     3901 GCTACGGCTT CTACCACTTC GGCACCTACC CCAGCGGCTA CGAGAACCCC TTCCTGCACG 
     3961 CCATCAACAA CGGCGGCTAC ACCAACACCC GCATCGAGAA GTACGAGGAC GGCGGCGTGC 
     4021 TGCACGTGAG CTTCAGCTAC CGCTACGAGG CCGGCCGCGT GATCGGCGAC TTCAAGGTGA 
     4081 TGGGCACCGG CTTCCCCGAG GACAGCGTGA TCTTCACCGA CAAGATCATC CGCAGCAACG 
     4141 CCACCGTGGA GCACCTGCAC CCCATGGGCG ATAACGATCT GGATGGCAGC TTCACCCGCA 
     4201 CCTTCAGCCT GCGCGACGGC GGCTACTACA GCTCCGTGGT GGACAGCCAC ATGCACTTCA 
     4261 AGAGCGCCAT CCACCCCAGC ATCCTGCAGA ACGGGGGCCC CATGTTCGCC TTCCGCCGCG 
     4321 TGGAGGAGGA TCACAGCAAC ACCGAGCTGG GCATCGTGGA GTACCAGCAC GCCTTCAAGA 
     4381 CCCCGGATGC AGATGCCGGT GAAGAAAGAG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4441 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4501 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4561 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4621 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4681 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4741 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4801 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4861 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4921 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4981 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5041 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCTTGGCTG 
     5101 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5161 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     5221 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 
     5281 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 
     5341 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG 
     5401 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 
     5461 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 
     5521 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 
     5581 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 
     5641 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 
     5701 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 
     5761 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 
     5821 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 
     5881 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 
     5941 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 
     6001 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 
     6061 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 
     6121 ATCGACTGTG GCCGGCTGGG TGTGGCCGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 
     6181 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 
     6241 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 
     6301 GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG 
     6361 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 
     6421 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 
     6481 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 
     6541 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 
     6601 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 
     6661 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 
     6721 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 
     6781 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 
     6841 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 
     6901 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 
     6961 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 
     7021 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 
     7081 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 
     7141 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 
     7201 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 
     7261 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 
     7321 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 
     7381 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 
     7441 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 
     7501 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 
     7561 AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 
     7621 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 
     7681 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 
     7741 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 
     7801 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 
     7861 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 
     7921 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 
     7981 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 
     8041 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 
     8101 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 
     8161 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 
     8221 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 
     8281 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 
     8341 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 
     8401 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 
     8461 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 
     8521 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 
     8581 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 
     8641 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 
     8701 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 
     8761 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 
     8821 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 
     8881 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 
     8941 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 
     9001 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 
     9061 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 
     9121 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 
     9181 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//