LOCUS       MG211054                9209 bp    DNA     circular  SYN 
DEFINITION  Bcan (tGFP-tagged) - Mouse brevican (Bcan)
ACCESSION   NM_007529
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3690..4394 
                     /label="GFP"  
     misc_feature    4441..5026 
                     /label="PolyA signal"  
     misc_feature    7118..7763 
                     /label="ColE1"  
     misc_feature    5480..6274 
                     /label="Neo"  
     misc_feature    5111..5413 
                     /label="SV40 ori"  
     misc_feature    7778..8638 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3669..3675 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3669 
                     /label="Bcan"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATTCCACTG CTTCTGTCCC TGCTGGCCGC TCTGGTCCTG ACCCAAGCCC 
     1081 CTGCCGCCCT CGCTGATGAC CTGAAAGAAG ACAGCTCGGA GGATCGAGCC TTCCGCGTGC 
     1141 GCATCGGTGC CACGCAGCTG CGGGGCGTGC TGGGCGGTGC CCTGGCCATC CCATGCCACG 
     1201 TCCACCACCT GCGGCCGCCG CACAGTCGCC GGGCCGCGCC GGGTTTTCCC CGGGTCAAGT 
     1261 GGACCTTCCT GTCCGGGGAC CGGGAGGTAG AGGTACTGGT GGCTCGTGGG CTGCGCGTCA 
     1321 AGGTAAACGA AGCCTACCGG TTCCGCGTGG CGCTGCCTGC CTACCCCGCA TCGCTCACGG 
     1381 ATGTGTCTCT AGTATTGAGC GAACTGCGGC CCAATGATTC CGGGGTCTAT CGCTGCGAGG 
     1441 TCCAGCACGG TATCGACGAC AGCAGTGATG CTGTGGAGGT CAAGGTCAAA GGGGTCGTCT 
     1501 TCCTCTACAG AGAGGGCTCT GCGCGCTATG CTTTCTCCTT TGCTGGAGCC CAGGAAGCCT 
     1561 GCGCTCGCAT AGGAGCCCGA ATCGCCACCC CGGAGCAGCT CTATGCTGCC TACCTCGGCG 
     1621 GCTATGAGCA GTGTGATGCA GGCTGGCTGT CCGACCAAAC TGTGAGGTAC CCCATCCAGA 
     1681 ACCCACGAGA GGCCTGCTCT GGAGACATGG ATGGCTATCC TGGCGTGCGG AACTACGGAG 
     1741 TGGTGGGTCC TGATGATCTC TATGATGTCT ACTGTTATGC CGAAGACCTA AATGGAGAAC 
     1801 TGTTCCTAGG CGCCCCTCCC AGCAAGCTGA CATGGGAGGA GGCTCGGGAC TACTGTCTGG 
     1861 AACGTGGTGC ACAGATCGCT AGCACAGGCC AGCTGTACGC AGCCTGGAAT GGTGGCCTGG 
     1921 ACAGATGTAG CCCTGGCTGG CTGGCTGATG GCAGCGTGCG CTATCCCATC ATCACACCCA 
     1981 GCCAACGCTG TGGGGGCGGC CTGCCAGGAG TCAAGACCCT CTTCCTCTTT CCCAACCAGA 
     2041 CTGGCTTCCC CAGCAAGCAG AACCGCTTCA ATGTCTACTG CTTCCGAGAC TCTGCCCATC 
     2101 CCTCTGCTTC CTCTGAGGCC TCTAGCCCAG CCTCAGATGG ACTTGAGGCC ATTGTCACAG 
     2161 TGACAGAAAA GCTGGAGGAA CTGCAGCTGC CTCAGGAAGC GATGGAGAGC GAGTCTCGTG 
     2221 GGGCCATCTA CTCCATCCCC ATCTCAGAAG ATGGGGGAGG AGGAAGCTCC ACCCCAGAAG 
     2281 ACCCAGCAGA GGCCCCCAGG ACTCCGCTAG AATCGGAAAC CCAATCCATT GCACCACCTA 
     2341 CCGAGTCCTC AGAAGAGGAA GGCGTAGCCC TGGAGGAAGA AGAAAGATTC AAAGACTTGG 
     2401 AGGCTCTGGA GGAAGAGAAG GAGCAGGAGG ACCTGTGGGT GTGGCCCAGA GAGCTCAGCA 
     2461 GCCCTCTCCC TACTGGCTCA GAAACAGAGC ATTCACTCTC CCAGGTGTCC CCACCAGCCC 
     2521 AGGCAGTTCT ACAGCTGGGT GCCTCACCTT CTCCTGGGCC TCCAAGGGTC CGTGGACCGC 
     2581 CTGCAGAGAC TTTGCTCCCC CCGAGGGAGG GAAGCCCCAC ATCTACTCCT GGTGGGGCAA 
     2641 GAGAAGTAGG GGGGGAAACT GGGAGCCCTG AGCTCTCTGG GGTTCCTCGA GAGAGCGAGG 
     2701 AGGCAGGGAG CTCCAGCTTG GAGGATGGCC CTTCCCTACT TCCAGCTACA TGGGCCCCTG 
     2761 TGGGTCCCAG GGAGCTGGAG ACCCCCTCAG AAGAGAAGTC TGGAAGAACT GTCCTGGCAG 
     2821 GCACCTCAGT GCAGGCCCAG CCAGTGCTGC CCACCGACAG TGCCAGCCAC GGTGGAGTGG 
     2881 CTGTGGCTCC CTCATCAGGT GACTGTATCC CCAGCCCCTG CCACAATGGT GGGACATGCT 
     2941 TGGAGGAGAA GGAGGGTTTC CGCTGCCTAT GTTTGCCAGG CTATGGGGGG GACCTGTGCG 
     3001 ATGTTGGCCT TCATTTCTGC AGCCCTGGCT GGGAGGCCTT CCAGGGAGCC TGCTACAAGC 
     3061 ACTTTTCCAC ACGAAGGAGT TGGGAGGAGG CAGAAAGTCA GTGCCGAGCG CTAGGTGCTC 
     3121 ATCTGACCAG CATCTGCACC CCTGAGGAGC AAGACTTTGT CAACGATCGA TACCGGGAGT 
     3181 ACCAGTGGAT TGGGCTCAAT GACAGGACCA TCGAGGGTGA CTTCTTGTGG TCAGATGGTG 
     3241 CCCCTCTGCT CTATGAAAAC TGGAACCCTG GGCAGCCTGA CAGCTACTTC CTGTCTGGGG 
     3301 AGAACTGTGT GGTCATGGTG TGGCATGACC AGGGACAGTG GAGTGATGTG CCCTGCAACT 
     3361 ACCATCTATC CTACACCTGC AAGATGGGGC TTGTGTCCTG TGGGCCTCCA CCACAGCTAC 
     3421 CCCTGGCTCA AATATTTGGT CGCCCTCGGC TGCGCTACGC GGTGGATACT GTGCTTCGAT 
     3481 ATCGATGCCG AGACGGGCTG GCTCAGCGCA ACCTGCCGTT GATCCGCTGC CAGGAGAATG 
     3541 GGCTTTGGGA GGCCCCTCAG ATTTCCTGTG TACCCCGGAG GCCTGGCCGT GCTCTGCGCT 
     3601 CCATGGACGC CCCAGAAGGA CCACGGGGAC AGCTCTCGAG GCACAGGAAG GCACCGTTGA 
     3661 CACCGCCCTC CAGTCTCACG CGTACGCGGC CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC 
     3721 TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC 
     3781 TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA 
     3841 CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT 
     3901 ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG 
     3961 GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT 
     4021 TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGATG GGCACCGGCT 
     4081 TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC 
     4141 ACCTGCACCC CATGGGCGAT AACGATCTGG ATGGCAGCTT CACCCGCACC TTCAGCCTGC 
     4201 GCGACGGCGG CTACTACAGC TCCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC 
     4261 ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGGATC 
     4321 ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCGGATGCAG 
     4381 ATGCCGGTGA AGAAAGAGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4441 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4501 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4561 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4621 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4681 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4741 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4801 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4861 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4921 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4981 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5041 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CTTGGCTGTG GAATGTGTGT 
     5101 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5161 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5221 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 
     5281 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 
     5341 TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 
     5401 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 
     5461 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 
     5521 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 
     5581 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 
     5641 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 
     5701 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 
     5761 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 
     5821 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 
     5881 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 
     5941 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 
     6001 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 
     6061 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 
     6121 CGGCTGGGTG TGGCCGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 
     6181 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 
     6241 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 
     6301 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 
     6361 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC 
     6421 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA 
     6481 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 
     6541 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA 
     6601 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 
     6661 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 
     6721 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 
     6781 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 
     6841 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 
     6901 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 
     6961 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 
     7021 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 
     7081 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 
     7141 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 
     7201 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 
     7261 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 
     7321 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 
     7381 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 
     7441 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 
     7501 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 
     7561 GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 
     7621 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 
     7681 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 
     7741 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 
     7801 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 
     7861 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 
     7921 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 
     7981 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 
     8041 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 
     8101 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 
     8161 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 
     8221 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 
     8281 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 
     8341 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 
     8401 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 
     8461 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 
     8521 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 
     8581 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 
     8641 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 
     8701 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 
     8761 CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 
     8821 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT 
     8881 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC 
     8941 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG 
     9001 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT 
     9061 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG 
     9121 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC 
     9181 TGATTTAACA AAAATTTAAC GCGAATTTT
//