LOCUS       MG211000                9152 bp    DNA     circular  SYN 
DEFINITION  Itch (tGFP-tagged) - Mouse itchy, E3 ubiquitin protein ligase (Itch)
ACCESSION   NM_008395
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3633..4337 
                     /label="GFP"  
     misc_feature    4384..4969 
                     /label="PolyA signal"  
     misc_feature    7061..7706 
                     /label="ColE1"  
     misc_feature    5423..6217 
                     /label="Neo"  
     misc_feature    5054..5356 
                     /label="SV40 ori"  
     misc_feature    7721..8581 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3612..3618 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3612 
                     /label="Itch"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGACAGT GGACCACAGC TTGATTCCAT GGGTAGTCTG ACCATGAAAT 
     1081 CTCAACTTCA GATCACTGTC ATCTCAGCCA AACTTAAAGA AAATAAAAAG AATTGGTTTG 
     1141 GACCAAGTCC TTATGTAGAA GTCACAGTAG ATGGACAGTC AAAGAAGACA GAAAAATGCA 
     1201 ATAATACAAA CAGTCCCAAG TGGAAGCAGC CCCTCACAGT TATTGTTACC CCTACGAGTA 
     1261 AATTATGTTT TCGTGTGTGG AGTCACCAGA CCCTGAAGTC TGATGTTTTA TTGGGAACTG 
     1321 CTGGATTAGA TATTTATGAA ACATTAAAGT CAAACAATAT GAAACTTGAA GAAGTAGTTA 
     1381 TGACTTTGCA GCTTGTAGGT GACAAAGAGC CAACAGAGAC GATGGGAGAT TTGTCAGTTT 
     1441 GTCTTGATGG GCTGCAAGTA GAAGCTGAGG TTGTTACTAA CGGTGAAACG TCATGCTCCG 
     1501 AGAGTACTAC ACAGAATGAT GATGGCTGCA GAACCAGAGA TGATACAAGA GTGAGCACAA 
     1561 ATGGATCAGA GGACCCTGAG GTTGCAGCGT CAGGGGAAAA CAAGAGGGCC AATGGGAACA 
     1621 ATTCTCCGTC CCTTTCAAAT GGTGGTTTTA AGCCTTCTAG ACCTCCTAGA CCTTCGAGAC 
     1681 CACCTCCACC CACCCCACGA AGACCAGCTT CTGTCAATGG CTCACCATCC ACGAATTCTG 
     1741 ACAGTGATGG ATCTAGTACA GGCTCCTTGC CACCAACAAA TACAAATGTA AATACAAGTA 
     1801 CATCTGAAGG AGCAACATCT GGATTAATAA TTCCTCTTAC TATATCTGGA GGCTCGGGCC 
     1861 CTAGGCCTCT GAATACTGTA AGCCAAGCTC CCCTACCACC TGGGTGGGAG CAGAGAGTAG 
     1921 ACCAGCATGG GCGTGTTTAC TATGTAGACC ATGTTGAAAA GCGAACAACA TGGGATAGAC 
     1981 CAGAACCTCT ACCTCCTGGC TGGGAACGGC GTGTGGACAA TATGGGACGT ATTTATTATG 
     2041 TTGATCATTT CACAAGAACC ACAACATGGC AGAGGCCAAC CTTGGAATCT GTCCGGAACT 
     2101 ATGAACAGTG GCAGCTACAG CGTAGTCAGC TTCAGGGAGC AATGCAGCAG TTTAACCAGA 
     2161 GATTCATCTA TGGGAATCAA GATTTGTTTG CTACATCACA AAACAAAGAA TTTGATCCGC 
     2221 TTGGTCCGTT GCCCCCTGGA TGGGAGAAGA GAACTGATAG CAACGGCAGA GTGTATTTTG 
     2281 TCAACCACAA CACTCGGATT ACTCAGTGGG AAGACCCCAG AAGCCAAGGT CAGTTAAATG 
     2341 AAAAGCCCTT ACCAGAAGGC TGGGAAATGA GATTCACAGT GGATGGAATT CCATATTTTG 
     2401 TGGACCACAA TAGAAGAGCA ACTACTTATA TAGATCCACG AACGGGAAAA TCAGCCTTAG 
     2461 ACAATGGGCC CCAGATAGCC TATGTGCGGG ACTTCAAGGC AAAAGTTCAG TATTTCCGGT 
     2521 TCTGGTGCCA GCAACTGGCC ATGCCACAGC ATATAAAGAT CACAGTGACA AGAAAAACAT 
     2581 TGTTTGAGGA TTCCTTTCAG CAGATCATGA GCTTCAGCCC GCAAGACCTG AGAAGACGTT 
     2641 TGTGGGTGAT TTTCCCAGGA GAAGAAGGTT TAGATTATGG AGGTGTAGCA AGAGAATGGT 
     2701 TCTTTCTTTT GTCACATGAA GTGTTGAACC CAATGTATTG CCTGTTTGAA TATGCAGGGA 
     2761 AGGATAACTA CTGCCTGCAG ATAAACCCCG CTTCTTACAT CAATCCAGAC CACCTGAAAT 
     2821 ACTTTCGTTT TATTGGCAGA TTTATTGCCA TGGCTCTGTT TCATGGGAAA TTCATAGATA 
     2881 CCGGATTTTC TTTACCATTC TATAAACGTA TCCTGAATAA ACCAGTTGGA CTGAAGGATT 
     2941 TAGAATCTAT TGATCCAGAA TTTTATAATT CGCTCATCTG GGTTAAAGAA AACAACATTG 
     3001 AAGAATGTGG TTTGGAAATG TACTTCTCAG TTGATAAAGA AATTCTAGGT GAAATTAAGA 
     3061 GTCATGATTT GAAACCCAAT GGTGGCAATA TTCTTGTGAC AGAAGAAAAC AAGGAGGAAT 
     3121 ACATCAGGAT GGTAGCTGAG TGGAGGTTGT CTCGAGGTGT TGAAGAACAG ACACAAGCTT 
     3181 TCTTTGAGGG CTTTAATGAA ATTCTTCCTC AGCAATATTT GCAATATTTT GATGCGAAGG 
     3241 AATTAGAGGT TCTTCTGTGT GGGATGCAGG AGATTGATTT GAATGACTGG CAGAGACATG 
     3301 CCATCTACCG CCACTACACC AGAACAAGCA AGCAGATCAT GTGGTTTTGG CAGTTTGTTA 
     3361 AAGAAATTGA TAATGAGAAG AGGATGAGAC TTCTGCAGTT TGTTACTGGA ACCTGCCGAT 
     3421 TGCCAGTGGG AGGATTTGCT GACCTTATGG GGAGTAATGG ACCACAGAAG TTCTGCATCG 
     3481 AAAAAGTTGG CAAAGAAAAT TGGTTACCCA GAAGCCATAC TTGTTTTAAC CGCCTGGACC 
     3541 TCCCACCTTA CAAGAGCTAT GAGCAACTGA AGGAAAAGCT GTTATTTGCC ATTGAAGAAA 
     3601 CTGAAGGATT TGGACAAGAG ACGCGTACGC GGCCGCTCGA GATGGAGAGC GACGAGAGCG 
     3661 GCCTGCCCGC CATGGAGATC GAGTGCCGCA TCACCGGCAC CCTGAACGGC GTGGAGTTCG 
     3721 AGCTGGTGGG CGGCGGAGAG GGCACCCCCG AGCAGGGCCG CATGACCAAC AAGATGAAGA 
     3781 GCACCAAAGG CGCCCTGACC TTCAGCCCCT ACCTGCTGAG CCACGTGATG GGCTACGGCT 
     3841 TCTACCACTT CGGCACCTAC CCCAGCGGCT ACGAGAACCC CTTCCTGCAC GCCATCAACA 
     3901 ACGGCGGCTA CACCAACACC CGCATCGAGA AGTACGAGGA CGGCGGCGTG CTGCACGTGA 
     3961 GCTTCAGCTA CCGCTACGAG GCCGGCCGCG TGATCGGCGA CTTCAAGGTG ATGGGCACCG 
     4021 GCTTCCCCGA GGACAGCGTG ATCTTCACCG ACAAGATCAT CCGCAGCAAC GCCACCGTGG 
     4081 AGCACCTGCA CCCCATGGGC GATAACGATC TGGATGGCAG CTTCACCCGC ACCTTCAGCC 
     4141 TGCGCGACGG CGGCTACTAC AGCTCCGTGG TGGACAGCCA CATGCACTTC AAGAGCGCCA 
     4201 TCCACCCCAG CATCCTGCAG AACGGGGGCC CCATGTTCGC CTTCCGCCGC GTGGAGGAGG 
     4261 ATCACAGCAA CACCGAGCTG GGCATCGTGG AGTACCAGCA CGCCTTCAAG ACCCCGGATG 
     4321 CAGATGCCGG TGAAGAAAGA GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4381 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4441 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4501 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4561 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4621 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4681 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4741 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4801 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4861 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4921 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4981 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCTTGGCT GTGGAATGTG 
     5041 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5101 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5161 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 
     5221 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 
     5281 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 
     5341 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 
     5401 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 
     5461 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 
     5521 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 
     5581 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 
     5641 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 
     5701 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 
     5761 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 
     5821 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 
     5881 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 
     5941 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 
     6001 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 
     6061 GGCCGGCTGG GTGTGGCCGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 
     6121 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 
     6181 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 
     6241 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 
     6301 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 
     6361 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 
     6421 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 
     6481 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 
     6541 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 
     6601 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 
     6661 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 
     6721 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 
     6781 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 
     6841 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     6901 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     6961 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     7021 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     7081 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     7141 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     7201 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     7261 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     7321 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     7381 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     7441 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     7501 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 
     7561 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 
     7621 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 
     7681 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 
     7741 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 
     7801 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 
     7861 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 
     7921 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 
     7981 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 
     8041 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 
     8101 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 
     8161 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 
     8221 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 
     8281 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 
     8341 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 
     8401 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 
     8461 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 
     8521 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 
     8581 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
     8641 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
     8701 TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 
     8761 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 
     8821 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 
     8881 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 
     8941 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG 
     9001 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT 
     9061 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG 
     9121 AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//