LOCUS       MG210874                9059 bp    DNA     circular  SYN 
DEFINITION  Nedd9 (tGFP-tagged) - Mouse neural precursor cell expressed, developmentally down-regulated gene 9 (Nedd9)
ACCESSION   NM_017464
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3540..4244 
                     /label="GFP"  
     misc_feature    4291..4876 
                     /label="PolyA signal"  
     misc_feature    6968..7613 
                     /label="ColE1"  
     misc_feature    5330..6124 
                     /label="Neo"  
     misc_feature    4961..5263 
                     /label="SV40 ori"  
     misc_feature    7628..8488 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3519..3525 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3519 
                     /label="Nedd9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGTACAAG AATCTTATGG CAAGGGCCTT GTATGACAAC GTCCCTGAGT 
     1081 GTGCTGAGGA GCTGGCCTTC CGCAAGGGAG ACATCTTAAC TGTCATAGAG CAGAACACAG 
     1141 GAGGGCTTGA GGGATGGTGG CTGTGTTCCC TCCACGGTCG CCAAGGCATT GTCCCAGGGA 
     1201 ACCGGGTGAA GCTTCTGATT GGTCCAGTGC AAGAGACCCC CGGTCATGAG CAGCCTACTC 
     1261 CTGGACCTAT GCATCAGACC TTTGGCCAAC AGAAACTCTA TCAAGTGCCA AATTCCCAGG 
     1321 CAGCATCTCG GGATACCATC TACCAAGTGC CACCCTCCTA CCAGAATCAG GGAATTTACC 
     1381 AAGTACCCAC TGGCCATGGC ACTCCAGAAC AAGATGTATA TCAAGTACCA CCATCAGTTC 
     1441 AGAGGAACAT TGGCGGCACT AATGGACCCC TTCTAAGCAA AAAGGTGATC ACCCCAGTGA 
     1501 GGACGGGCCA TGGCTATGTG TACGAGTACC CATCCAGATA CCAAAAGGAT GTCTACGATG 
     1561 TCCCTCCTTC CCACAGCACT CAAGGGGTAT ATGACATCCC TCCTTCCTCA GTAAAAGGCC 
     1621 CTGTGTTTTC AGTTCCAGTG GGAGAGATAA AACCTCAAGG GGTATATGAC ATTCCCCCCA 
     1681 CCCAAGGGGT CTATGCCATT CCACCATCGG CTTGCCGAGA TGAGGCAGGG CTCAGGGAAA 
     1741 AGGAATATGA TTTCCCTCCT CCAATGAAGC AAGATGGAAA ACCAGACACC AGACCTGAGG 
     1801 GGGTTTATGA CATCCCTCCA ACCAGCACCA AGACAGCAGG CAAGGACCTT CACATCAAAT 
     1861 TCCCCTGTGA TGCTCCAGGA GGTGTCGAAC CAATGGCACG AAGACACCAG AGCTTTTCCC 
     1921 TGCACCATGC ACCCTCTCAG CTGGGACAGT CTGGGGACAC TCAGAGTGAT GCCTATGATG 
     1981 TCCCCCGGGG AGTTCAGTTT CTGGAGGTAC CAACAGAAAC CAGTGAAAAG GCAAATCCGG 
     2041 AGGAAAGAGA CGGTGTCTAC GATGTCCCTC TGCACAACCC AGCAGATGCC AAAGGCTCTC 
     2101 GGGACGTGGT AGATGGGATC AACAGACTGT CTTTCTCCAG CACTGGCAGT ACCAGGAGTA 
     2161 ACATGTCCAC CTCTTCCACC TCCTCAAAGG AGTCTTCACT GTCAGCCTCC CCGTCTCAAG 
     2221 ACAAAAGGCT CCGACTGGAC CCAGACACAG CCATAGAGAA GCTCTATCGG CTCCAGCAGA 
     2281 CCCTGGAGAT GGGTGTGTGC AGCCTCATGT CACTGGTCAC CACAGACTGG AGGTGCTACG 
     2341 GATACATGGA AAGGCACATC AATGAGATCC GCACCGCGGT GGACAAAGTA GAGCTGTTCT 
     2401 TACGAGAATA CCTCCATTTT GCCAAGGGAG CTTTAGCCAA TGCCTCCTGC CTCCCAGAAC 
     2461 TGGTCCTCCA CAACAAAATG AAGCGGGAAC TCCAAAGAGT AGAAGATTCC CACCAGATTC 
     2521 TAAGCCAAAC CAGCCATGAC TTGAATGAAT GCAGCTGGTC CCTGAATATT TTAGCTATCA 
     2581 ATAAGCCCCA AAATAAGTGT GATGACCTAG ACCGGTTTGT GATGGTCGCC AAGACAGTGC 
     2641 CAGACGACGC CAAACAACTG ACCACCACCA TCAGCACCTA CGCGGAGACC CTCTTTAGAG 
     2701 CAGATCCTGC CAATTCCCAT CTGAAGAATG GGCCCAACAG CATCATGAAC TCAAGCGAGT 
     2761 ACACACATCC GGGCTCCCAG ATGCAGCCAC TGCATCCTGG TGACTACAAA GCCCAGGTCC 
     2821 ACAGTAAGCC GTTGCCTCCT AGTCTAAGCA AGGACCAGCC ACCAGACTGC GGTAGCAGTG 
     2881 ACGGTTCTGA GCGGAGTTGG ATGGATGATT ATGATTATGT TCACCTACAG GGCAAGGAGG 
     2941 AGTTTGAGCG ACAGCAGAAG GAGCTCTTGG AAAAGGAGAA CATCATGAAG CAGAGTAAGG 
     3001 CGCAGCTGGA GCATCACCAG CTGAGTCAGT TCCAGCTGTT GGAACAAGAG ATCACCAAGC 
     3061 CTGTGGAGAA TGACATCTCT AAATGGAAGC CCTCTCAGAG CCTCCCAACC ACCAACAACA 
     3121 GTGTGGGTGC TCAGGATAGG CAGTTGCTTT GCTTCTACTA TGACCAGTGC GAGACCCATT 
     3181 TCATTTCCCT ACTCAACGCC ATCGACGCCC TCTTCAGCTG CGTCAGCTCA GCCCAACCCC 
     3241 CACGGATCTT TGTGGCGCAC AGCAAGTTTG TCATTCTTAG TGCGCACAAA CTGGTGTTCA 
     3301 TTGGAGACAC TCTGACAAGG CAGGTGGCTG CCCAGGACAT TCGCAACAAA GTCAGGAACT 
     3361 CCAGCAACCA GCTCTGCGAA CAGCTCAAGA CGATAGTGAT GGCGACCAAA ATGGCCGCCC 
     3421 TCCACTACCC CAGTACCACC GCCTTGCAGG AAATGGTGCA CCAGGTGACA GACCTGTCCA 
     3481 GAAATGCTCA GCTGTTTAAG CGTTCCTTGC TGGAGATGGC CACCTTTACG CGTACGCGGC 
     3541 CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC TGCCCGCCAT GGAGATCGAG TGCCGCATCA 
     3601 CCGGCACCCT GAACGGCGTG GAGTTCGAGC TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC 
     3661 AGGGCCGCAT GACCAACAAG ATGAAGAGCA CCAAAGGCGC CCTGACCTTC AGCCCCTACC 
     3721 TGCTGAGCCA CGTGATGGGC TACGGCTTCT ACCACTTCGG CACCTACCCC AGCGGCTACG 
     3781 AGAACCCCTT CCTGCACGCC ATCAACAACG GCGGCTACAC CAACACCCGC ATCGAGAAGT 
     3841 ACGAGGACGG CGGCGTGCTG CACGTGAGCT TCAGCTACCG CTACGAGGCC GGCCGCGTGA 
     3901 TCGGCGACTT CAAGGTGATG GGCACCGGCT TCCCCGAGGA CAGCGTGATC TTCACCGACA 
     3961 AGATCATCCG CAGCAACGCC ACCGTGGAGC ACCTGCACCC CATGGGCGAT AACGATCTGG 
     4021 ATGGCAGCTT CACCCGCACC TTCAGCCTGC GCGACGGCGG CTACTACAGC TCCGTGGTGG 
     4081 ACAGCCACAT GCACTTCAAG AGCGCCATCC ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA 
     4141 TGTTCGCCTT CCGCCGCGTG GAGGAGGATC ACAGCAACAC CGAGCTGGGC ATCGTGGAGT 
     4201 ACCAGCACGC CTTCAAGACC CCGGATGCAG ATGCCGGTGA AGAAAGAGTT TAAACGGCCG 
     4261 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4321 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4381 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4441 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4501 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4561 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4621 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4681 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4741 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4801 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4861 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4921 ACGCGGCCAG CTTGGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC 
     4981 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG 
     5041 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 
     5101 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT 
     5161 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT 
     5221 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC 
     5281 CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC 
     5341 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC 
     5401 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA 
     5461 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG 
     5521 CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG 
     5581 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG 
     5641 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG 
     5701 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC 
     5761 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA 
     5821 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC 
     5881 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT 
     5941 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCCGACCG CTATCAGGAC 
     6001 ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC 
     6061 CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT 
     6121 GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC 
     6181 TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG 
     6241 TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG 
     6301 CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA 
     6361 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA 
     6421 ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT 
     6481 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG 
     6541 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT 
     6601 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG 
     6661 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG 
     6721 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 
     6781 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 
     6841 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 
     6901 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 
     6961 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 
     7021 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 
     7081 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 
     7141 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 
     7201 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 
     7261 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 
     7321 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 
     7381 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA 
     7441 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 
     7501 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 
     7561 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 
     7621 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 
     7681 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 
     7741 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 
     7801 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 
     7861 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 
     7921 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 
     7981 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 
     8041 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 
     8101 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 
     8161 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 
     8221 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 
     8281 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 
     8341 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 
     8401 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 
     8461 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 
     8521 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 
     8581 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA 
     8641 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA 
     8701 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT 
     8761 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC 
     8821 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT 
     8881 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC 
     8941 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC 
     9001 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//