LOCUS       MG210866                9056 bp    DNA     circular  SYN 
DEFINITION  Grik1 (tGFP-tagged) - Mouse glutamate receptor, ionotropic, kainate 1 (cDNA clone MGC:25101 IMAGE:4501004)
ACCESSION   BC031822
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3537..4241 
                     /label="GFP"  
     misc_feature    4288..4873 
                     /label="PolyA signal"  
     misc_feature    6965..7610 
                     /label="ColE1"  
     misc_feature    5327..6121 
                     /label="Neo"  
     misc_feature    4958..5260 
                     /label="SV40 ori"  
     misc_feature    7625..8485 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3516..3522 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3516 
                     /label="Grik1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCAATACC ACATTAACCT ATGACATCCA GAGAATTAAT CTTTTTGATA 
     1081 GTTTTGAAGC CTCCCGAAGA GCATGCGACC AACTGGCTCT TGGGGTGGCC GCCCTCTTCG 
     1141 GTCCTTCCCA CAGCTCCTCC GTCAGTGCTG TACAGTCTAT TTGCAATGCT CTGGAAGTTC 
     1201 CACACATTCA GACTCGCTGG AAACACCCTT CTGTGGACAA CAGAGACTTA TTTTACATCA 
     1261 ACCTCTACCC AGATTATGCA GCTATCAGCA GGGCAGTCCT GGATCTGGTC CTCTATTACA 
     1321 ACTGGAAAAC AGTGACGGTG GTGTACGAAG ACAGCACAGG TCTAATTCGT CTGCAAGAGC 
     1381 TCATCAAAGC TCCCTCCAGA TACAACATCA AAATCAAAAT CCGCCAGCTT CCCTCTGGCA 
     1441 ATAAGGATGC CAAACCTCTG CTCAAGGAGA TGAAGAAAGG CAAGGAGTTC TATGTGATAT 
     1501 TTGATTGTTC GCACGAGACG GCTGCTGAAA TTCTTAAGCA GATTTTGTTC ATGGGCATGA 
     1561 TGACTGAATA CTATCACTAC TTCTTCACAA CCCTGGACTT GTTTGCTTTG GATCTGGAAC 
     1621 TCTACAGGTA CAGTGGTGTA AATATGACTG GATTTCGGTT GCTGAATATT GACAACCCTC 
     1681 ACGTGTCATC CATCATTGAG AAGTGGTCCA TGGAGAGATT GCAGGCCCCA CCCAGACCTG 
     1741 AGACTGGTCT CCTGGACGGC GTGATGACAA CTGAAGCAGC TCTGATGTAC GATGCTGTGT 
     1801 ACATGGTAGC CATCGCCTCT CACCGTGCCT CTCAGCTGAC CGTCAGTTCC CTGCAGTGCC 
     1861 ATCGACATAA GCCATGGCGC CTAGGACCCA GATTTATGAA CCTCATCAAA GAGGCGCGGT 
     1921 GGGATGGCTT GACGGGGCGG ATCACCTTCA ATAAGACGGA TGGCTTGAGA AAGGATTTTG 
     1981 ACCTGGACAT TATCAGTCTC AAAGAGGAAG GAACTGAAAA GATTGGGATT TGGAACTCCA 
     2041 ACAGTGGGCT GAACATGACG GATGGCAACA GAGACAGGTC CAACAATATC ACAGATTCGC 
     2101 TGGCTAACCG AACGCTCATT GTCACCACTA TTCTGGAAGA GCCCTACGTG ATGTACAGGA 
     2161 AATCCGATAA ACCACTGTAC GGAAATGACA GATTTGAAGG ATATTGCCTG GATCTGCTGA 
     2221 AAGAACTGTC AAATATCCTA GGTTTCCTTT ATGATGTTAA ACTGGTTCCT GACGGCAAAT 
     2281 ATGGAGCCCA GAATGACAAA GGGGAGTGGA ACGGGATGGT TAAGGAACTC ATCGACCACA 
     2341 GAGCTGACCT AGCAGTGGCC CCTCTCACCA TCACGTATGT ACGGGAGAAA GTCATTGACT 
     2401 TCTCCAAGCC TTTCATGACT CTGGGCATTA GCATCCTTTA CCGGAAGCCC AATGGAACCA 
     2461 ACCCCGGCGT CTTCTCCTTC CTCAACCCCC TGTCTCCAGA CATTTGGATG TATGTGCTCC 
     2521 TCGCTTGCCT AGGAGTCAGT TGTGTGCTTT TTGTGATTGC AAGGTTCACA CCCTACGAGT 
     2581 GGTATAACCC CCACCCGTGC AACCCTGACT CAGACGTGGT GGAAAACAAT TTCACTTTGC 
     2641 TAAATAGTTT CTGGTTTGGC GTTGGAGCTC TCATGCGGCA AGGATCGGAG CTGATGCCCA 
     2701 AGGCTCTATC GACCAGAATA GTTGGAGGAA TATGGTGGTT TTTCACCCTA ATCATCATCT 
     2761 CATCCTACAC TGCCAACCTG GCTGCCTTCT TGACAGTAGA AAGGATGGAA TCCCCCATCG 
     2821 ATTCCGCAGA CGACCTGGCC AAACAAACCA AGATAGAATA CGGGGCAGTC AGAGATGGCT 
     2881 CGACAATGAC CTTCTTCAAG AAATCAAAAA TCTCCACGTA TGAGAAAATG TGGGCTTTCA 
     2941 TGAGCAGTAG ACAGCAGAGC GCCCTGGTTA AAAACAGCGA TGAGGGGATC CAAAGGGTGC 
     3001 TTACCACCGA CTACGCCCTG CTGATGGAGT CCACCAGCAT TGAGTATGTG ACACAGAGGA 
     3061 ACTGCAACCT CACTCAGATC GGGGGCCTCA TAGACTCCAA AGGCTATGGA GTGGGGACAC 
     3121 CTATCGGCTC CCCTTACCGG GATAAAATTA CAATTGCTAT TCTTCAACTA CAAGAAGAAG 
     3181 GGAAGCTTCA TATGATGAAA GAGAAATGGT GGAGGGGAAA TGGCTGCCCT GAAGAAGACA 
     3241 GTAAAGAAGC CAGTGCTCTA GGAGTGGAAA ATATCGGGGG TATCTTCATT GTTCTGGCTG 
     3301 CAGGACTCGT CCTTTCTGTG TTTGTAGCCA TTGGAGAATT CATATACAAA TCACGGAAGA 
     3361 ACAATGACAT TGAGCAGGCT TTTTGTTTCT TTTATGGACT GCAGTGTAAA CAAACCCATC 
     3421 CGACCAACTC CACTTCCGGG ACTACATTAT CTACAGATGT AGAGTGTGGC AAATTATTAC 
     3481 GAGAGGAAAA GGGGATTCGG ACACAGCCCT CAGTCCATAC TGTAACGCGT ACGCGGCCGC 
     3541 TCGAGATGGA GAGCGACGAG AGCGGCCTGC CCGCCATGGA GATCGAGTGC CGCATCACCG 
     3601 GCACCCTGAA CGGCGTGGAG TTCGAGCTGG TGGGCGGCGG AGAGGGCACC CCCGAGCAGG 
     3661 GCCGCATGAC CAACAAGATG AAGAGCACCA AAGGCGCCCT GACCTTCAGC CCCTACCTGC 
     3721 TGAGCCACGT GATGGGCTAC GGCTTCTACC ACTTCGGCAC CTACCCCAGC GGCTACGAGA 
     3781 ACCCCTTCCT GCACGCCATC AACAACGGCG GCTACACCAA CACCCGCATC GAGAAGTACG 
     3841 AGGACGGCGG CGTGCTGCAC GTGAGCTTCA GCTACCGCTA CGAGGCCGGC CGCGTGATCG 
     3901 GCGACTTCAA GGTGATGGGC ACCGGCTTCC CCGAGGACAG CGTGATCTTC ACCGACAAGA 
     3961 TCATCCGCAG CAACGCCACC GTGGAGCACC TGCACCCCAT GGGCGATAAC GATCTGGATG 
     4021 GCAGCTTCAC CCGCACCTTC AGCCTGCGCG ACGGCGGCTA CTACAGCTCC GTGGTGGACA 
     4081 GCCACATGCA CTTCAAGAGC GCCATCCACC CCAGCATCCT GCAGAACGGG GGCCCCATGT 
     4141 TCGCCTTCCG CCGCGTGGAG GAGGATCACA GCAACACCGA GCTGGGCATC GTGGAGTACC 
     4201 AGCACGCCTT CAAGACCCCG GATGCAGATG CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC 
     4261 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4321 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4381 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4441 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4501 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4561 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4621 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4681 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4741 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4801 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4861 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4921 CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 
     4981 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC 
     5041 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA 
     5101 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG 
     5161 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG 
     5221 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG 
     5281 GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 
     5341 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 
     5401 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 
     5461 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 
     5521 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 
     5581 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 
     5641 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 
     5701 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 
     5761 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 
     5821 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC 
     5881 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 
     5941 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CCGACCGCTA TCAGGACATA 
     6001 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 
     6061 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 
     6121 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC 
     6181 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 
     6241 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 
     6301 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 
     6361 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 
     6421 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT 
     6481 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 
     6541 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 
     6601 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 
     6661 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 
     6721 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 
     6781 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 
     6841 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 
     6901 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 
     6961 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 
     7021 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 
     7081 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 
     7141 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 
     7201 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 
     7261 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA 
     7321 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 
     7381 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA 
     7441 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 
     7501 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 
     7561 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 
     7621 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 
     7681 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 
     7741 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 
     7801 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 
     7861 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 
     7921 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 
     7981 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 
     8041 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 
     8101 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 
     8161 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 
     8221 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 
     8281 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 
     8341 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 
     8401 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 
     8461 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 
     8521 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 
     8581 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG 
     8641 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 
     8701 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 
     8761 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 
     8821 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 
     8881 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 
     8941 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA 
     9001 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//