LOCUS       MG210810                9029 bp    DNA     circular  SYN 
DEFINITION  Bicd2 (tGFP-tagged) - Mouse bicaudal D homolog 2 (Drosophila) (Bicd2), transcript variant 2
ACCESSION   NM_029791
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3508..4212 
                     /label="GFP"  
     misc_feature    4259..4844 
                     /label="PolyA signal"  
     misc_feature    6936..7581 
                     /label="ColE1"  
     misc_feature    5298..6092 
                     /label="Neo"  
     misc_feature    4929..5231 
                     /label="SV40 ori"  
     misc_feature    7596..8456 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3480..3487 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3480 
                     /label="Bicd2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCGCGCCG TCGGAGGAGG AGGAGTATGC GCGTCTGGTG ATGGAGGCGC 
     1081 AGCCGGAGTG GCTGCGCGCC GAGGTGAAGC GGCTGTCCCA CGAGCTGGCC GAGACCACGC 
     1141 GTGAGAAGAT CCAGGCGGCC GAGTATGGAC TGGCGGTGCT GGAGGAGAAG CACCAGCTCA 
     1201 AGCTGCAGTT CGAGGAGCTC GAGGTGGACT ATGAGGCCAT CCGCAGCGAG ATGGAGCAGC 
     1261 TCAAAGAGGC ATTTGGCCAG GCACATACAA ACCACAAGAA GGTGGCTGCT GATGGTGAGA 
     1321 GCCGGGAGGA GAGCCTGATC CAGGAGTCGG CCTCCAAGGA GCAGTACTAC GTGCGGAAGG 
     1381 TGCTGGAGCT GCAGACAGAG CTGAAACAGC TGCGCAACGT CCTCACCAAC ACCCAGTCTG 
     1441 AGAATGAGCG CCTCACGTCT GTGGCCCAGG AGCTAAAAGA GATCAACCAG AATGTGGAGA 
     1501 TCCAGCGTGG TCGCCTGCGG GATGACATCA AGGAGTACAA GTTCCGGGAG GCCCGTCTAC 
     1561 TTCAGGACTA CTCAGAGCTG GAGGAGGAGA ACATCAGCCT GCAGAAACAA GTGTCTGTGC 
     1621 TCAGGCAGAA CCAGGTGGAG TTTGAGGGCC TCAAGCATGA AATCAAGCGC CTGGAGGAGG 
     1681 AGACAGAGTA CCTCAACAGC CAGCTAGAGG ATGCCATCCG GCTTAAGGAG ATCTCTGAAC 
     1741 GGCAGTTGGA GGAGGCGCTG GAGACACTGA AGACAGAGCG GGAGCAGAAG AACAACCTGC 
     1801 GCAAGGAGTT GTCGCACTAC ATGAGCATCA ACGATTCCTT CTATACCAGC CACCTGCAGG 
     1861 TCTCCTTAGA TGGCCTCAAG TTCAGTGATG ATACTGTCAC CGCAGAGCCC AACAATGACG 
     1921 CCGAAGCCCT GGTCAATGGC TTTGAGCACA GTGGCTTGGT CAAATCATCC TTGGACAACA 
     1981 AGACATCCAC ACCCAGGAAG GATGGCCTAG CTCCACCCTC CCCCAGCCTC GTCTCTGACC 
     2041 TGCTCAGTGA GCTCCACATA TCTGAGATCC AGAAGCTGAA ACAGCAGCTG GTGCAGATGG 
     2101 AGCGGGAGAA GGTGGGCCTG CTGGCAACAC TGCAGGACAC ACAGAAGCAG CTGGAGCAGG 
     2161 CTCGGGGAAC CCTCTCTGAG CAGCATGAGA AGGTGAATCG TCTCACAGAG AACCTTAGTG 
     2221 CCCTCCGGCG CCTGCAGGCT GGCAAGGAGC GACAGACCTC GTTGGATAAT GAGAAGGACC 
     2281 GTGACAGCCA CGAAGACGGT GACTACTATG AGGTGGACAT CAATGGGCCT GAGATCCTGG 
     2341 CCTGCAAGTA CCACGTGGCT GTGGCTGAGG CTGGCGAGCT CCGGGAGCAG CTCAAGGCGT 
     2401 TGCGCAGCAC ACACGAAGCT CGCGAAGCCC AGCACGCAGA AGAAAAGGGC CGGTATGAGG 
     2461 CTGAGGGCCA GGCACTCACT GAGAAGATCT CTCTGCTGGA GAAGGCTAGC CACCAGGACC 
     2521 GAGAGCTGCT GGCCCATCTG GAAAAGGAGC TGAAGAAGGT GAGCGATGTG GCTGGTGAGA 
     2581 CCCAGGGCAG CCTGAATGTG GCTCAGGATG AGCTGGTGAC CTTCAGCGAG GAGCTGGCCA 
     2641 ACCTCTACCA CCATGTGTGC ATGTGCAACA ACGAGACGCC CAACCGTGTC ATGCTCGACT 
     2701 ATTATCGTGA GGGCCAGGGC AAGGCTGGCC GCACCAGCCC AGAGGGCCGC GGGCGCCGGT 
     2761 CACCTGTCCT CTTGCCCAAG GGGCTGTTGG CCACAGAGGT GGGCCGAGCA GATGGTGGGA 
     2821 CTGGGGACAA CAGTCCTTCA CCCAGTTCTT CACTGCCGTC ACCTCTGAGT GACCCCCGCC 
     2881 GGGAACCTAT GAACATCTAC AACCTGATCG CCATCATCCG AGACCAGATC AAGCACCTGC 
     2941 AGGCAGCCGT GGACCGTACG ACCGAGCTGT CCCGGCAGCG GATTGCCTCG CAGGAGCTGG 
     3001 GCCCTGCTGT GGACAAGGAC AAGGAAGCAC TCATGGAGGA GATCCTCAAG CTGAAGTCCC 
     3061 TGCTGAGTAC CAAGCGGGAG CAGATCACCA CACTGCGCAC CGTGCTCAAG GCCAACAAGC 
     3121 AGACAGCTGA GGTGGCCCTG GCCAACCTGA AGAGCAAGTA TGAGAACGAG AAGGCCATGG 
     3181 TGACCGAGAC CATGATGAAG CTACGGAACG AGCTCAAGGC CCTCAAGGAA GACGCAGCCA 
     3241 CCTTCTCCTC CCTGCGTGCC ATGTTTGCCA CCAGGTGTGA CGAATACATC ACACAGCTGG 
     3301 ATGAGATGCA GCGGCAGCTG GCAGCTGCCG AGGACGAGAA GAAGACCCTT AACTCTCTGT 
     3361 TGCGCATGGC CATCCAGCAG AAGCTGGCGC TCACCCAGCG GCTGGAGCTG CTGGAGCTGG 
     3421 ACCATGAACA GACCCGCAGG GGCCGCAGCA AGGCCGCCTC CAAGGCCAAG CCAGCCTCAC 
     3481 CGAGCCTGAG CGGACCGACG CGTACGCGGC CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC 
     3541 TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC 
     3601 TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA 
     3661 CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT 
     3721 ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG 
     3781 GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT 
     3841 TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGATG GGCACCGGCT 
     3901 TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC 
     3961 ACCTGCACCC CATGGGCGAT AACGATCTGG ATGGCAGCTT CACCCGCACC TTCAGCCTGC 
     4021 GCGACGGCGG CTACTACAGC TCCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC 
     4081 ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGGATC 
     4141 ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCGGATGCAG 
     4201 ATGCCGGTGA AGAAAGAGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4261 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4321 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4381 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4441 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4501 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4561 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4621 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4681 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4741 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4801 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4861 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CTTGGCTGTG GAATGTGTGT 
     4921 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     4981 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5041 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 
     5101 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 
     5161 TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 
     5221 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 
     5281 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 
     5341 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 
     5401 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 
     5461 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 
     5521 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 
     5581 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 
     5641 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 
     5701 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 
     5761 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 
     5821 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 
     5881 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 
     5941 CGGCTGGGTG TGGCCGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 
     6001 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 
     6061 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 
     6121 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 
     6181 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC 
     6241 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA 
     6301 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 
     6361 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA 
     6421 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 
     6481 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 
     6541 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 
     6601 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 
     6661 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 
     6721 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 
     6781 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 
     6841 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 
     6901 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 
     6961 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 
     7021 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 
     7081 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 
     7141 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 
     7201 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 
     7261 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 
     7321 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 
     7381 GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 
     7441 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 
     7501 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 
     7561 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 
     7621 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 
     7681 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 
     7741 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 
     7801 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 
     7861 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 
     7921 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 
     7981 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 
     8041 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 
     8101 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 
     8161 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 
     8221 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 
     8281 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 
     8341 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 
     8401 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 
     8461 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 
     8521 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 
     8581 CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 
     8641 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT 
     8701 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC 
     8761 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG 
     8821 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT 
     8881 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG 
     8941 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC 
     9001 TGATTTAACA AAAATTTAAC GCGAATTTT
//