LOCUS       MG210781                8993 bp    DNA     circular  SYN 
DEFINITION  Tmprss6 (tGFP-tagged) - Mouse transmembrane serine protease 6 (Tmprss6)
ACCESSION   NM_027902
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3474..4178 
                     /label="GFP"  
     misc_feature    4225..4810 
                     /label="PolyA signal"  
     misc_feature    6902..7547 
                     /label="ColE1"  
     misc_feature    5264..6058 
                     /label="Neo"  
     misc_feature    4895..5197 
                     /label="SV40 ori"  
     misc_feature    7562..8422 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3453..3459 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3453 
                     /label="Tmprss6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGAGATGT TTCCAGCTCC CCTGTTCTAC CAGGATGCCC ACCACCGAGG 
     1081 TCCCCCAAGC GGCTGATGGT CAGGGCGATG CGGGTGATGG AGAGGAAGCT GCTGAGCCAG 
     1141 AGGGGAAGTT CAAGCCCCCA AAAAACACCA AGAGAAAAAA CCGGGACTAC GTCCGCTTCA 
     1201 CGCCACTGTT GCTGGTCTTG GCTGCGCTGG TCTCAGCAGG GGTCATGCTT TGGTATTTCC 
     1261 TAGGGTACAA AGCGGAAGTG ACCGTAAGCC AGGTGTACTC TGGCAGCCTC CGGGTGCTCA 
     1321 ACCGTCATTT CTCCCAGGAC CTGGGCCGAC GGGAGTCTAT TGCTTTCCGC AGTGAATCTG 
     1381 CCAAAGCCCA GAAGATGCTC CAAGAACTGG TTGCCAGCAC CCGCCTGGGT ACTTACTACA 
     1441 ACTCTAGTTC TGTCTACTCC TTTGGGGAGG GACCCCTCAC CTGCTTCTTC TGGTTTATCC 
     1501 TTGACATCCC TGAGTACCAG CGACTGACCC TGAGCCCTGA AGTAGTGCGC GAGCTCCTGG 
     1561 TGGATGAGCT ACTGTCCAAC AGCTCAACCC TGGCTTCCTA TAAGACCGAA TATGAGGTGG 
     1621 ACCCGGAAGG CCTGGTGATC CTGGAAGCCA GTGTGAACGA CATAGTCGTA CTGAATTCCA 
     1681 CGCTGGGCTG TTATCGCTAC AGCTATGTGA ACCCAGGCCA GGTCCTCCCA TTGAAGGGGC 
     1741 CTGACCAGCA GACCACAAGC TGCCTGTGGC ATCTGCAAGG GCCCGAAGAC CTCATGATCA 
     1801 AAGTGCGGCT GGAGTGGACC CGGGTCGATT GCAGAGACAG GGTGGCGATG TACGACGCAG 
     1861 CTGGGCCCCT GGAGAAGAGA CTTATCACCT CGGTCTATGG GTGCAGCCGC CAGGAACCTG 
     1921 TGATGGAGGT GCTGGCATCG GGCTCCGTCA TGGCCGTGGT GTGGAAAAAG GGCATGCATA 
     1981 GCTACTATGA CCCTTTCCTG CTCTCAGTGA AGTCTGTGGC CTTCCAGGAC TGCCAGGTGA 
     2041 ACCTGACACT GGAGGGCCGG CTGGACACAC AGGGCTTCCT CCGTACACCC TACTACCCCA 
     2101 GTTACTACTC TCCCAGTACC CACTGCTCCT GGCATCTCAC GGTACCCTCT CTGGACTACG 
     2161 GCTTGGCGCT CTGGTTCGAT GCCTACGCAC TGAGGAGGCA GAAGTACAAC CGACTGTGTA 
     2221 CTCAGGGCCA GTGGATGATC CAGAACAGGA GGCTGTGTGG CTTCCGTACC CTGCAGCCAT 
     2281 ATGCTGAGAG GATCCCCATG GTGGCCTCAG ATGGTGTCAC CATCAACTTC ACCTCCCAGA 
     2341 TCTCCCTCAC AGGCCCGGGT GTGCAAGTGT ACTACAGCTT GTACAACCAA TCAGACCCCT 
     2401 GCCCTGGTGA GTTCCTCTGC TCTGTGAATG GACTGTGTGT CCCTGCGTGT GACGGGATCA 
     2461 AGGACTGCCC CAATGGCCTG GATGAGAGAA ACTGTGTCTG CAGAGCCATG TTCCAGTGCC 
     2521 AAGAGGACAG CACGTGCATT TCACTGCCTA GAGTCTGTGA CCGGCAGCCC GACTGTCTCA 
     2581 ATGGCAGTGA CGAAGAACAG TGCCAAGAAG GAGTGCCCTG TGGGACATTC ACTTTCCAGT 
     2641 GTGAGGACCG GAGCTGTGTG AAGAAGCCCA ACCCAGAGTG TGACGGCCAG TCAGATTGCA 
     2701 GAGACGGCTC AGATGAGCAA CACTGTGACT GTGGCCTCCA GGGCCTCTCC AGCCGTATTG 
     2761 TGGGCGGGAC CGTGTCCTCC GAGGGTGAGT GGCCATGGCA GGCCAGCCTC CAGATTCGGG 
     2821 GTCGACACAT CTGTGGGGGG GCTCTCATCG CTGACCGCTG GGTCATAACG GCCGCCCACT 
     2881 GCTTCCAGGA GGACAGCATG GCCTCCCCGA AGCTGTGGAC CGTGTTCCTG GGAAAGATGC 
     2941 GGCAGAACTC GCGCTGGCCA GGCGAGGTGT CCTTCAAGGT GAGCCGTCTG TTCCTGCACC 
     3001 CGTACCACGA GGAGGACAGC CATGACTACG ACGTGGCCCT GCTGCAGCTC GACCACCCCG 
     3061 TGGTGTACTC GGCCACTGTG CGCCCCGTCT GCCTGCCTGC CCGCTCCCAC TTCTTTGAGC 
     3121 CAGGCCAGCA CTGCTGGATC ACAGGCTGGG GAGCCCAGCG AGAGGGTGGT CCGGTGAGCA 
     3181 ACACCCTGCA GAAGGTGGAC GTACAGCTGG TCCCTCAGGA CCTCTGCAGT GAGGCCTACC 
     3241 GCTACCAGGT GTCCCCACGC ATGCTCTGTG CTGGCTACCG CAAGGGCAAG AAAGATGCCT 
     3301 GCCAGGGTGA CTCTGGAGGC CCACTGGTTT GCAGGGAGCC CAGTGGCCGC TGGTTCCTGG 
     3361 CAGGGTTGGT TAGCTGGGGC CTGGGCTGTG GCCGACCCAA TTTCTTTGGC GTCTACACCC 
     3421 GTGTCACACG TGTGATCAAC TGGATCCAGC AGGTGCTGAC CACGCGTACG CGGCCGCTCG 
     3481 AGATGGAGAG CGACGAGAGC GGCCTGCCCG CCATGGAGAT CGAGTGCCGC ATCACCGGCA 
     3541 CCCTGAACGG CGTGGAGTTC GAGCTGGTGG GCGGCGGAGA GGGCACCCCC GAGCAGGGCC 
     3601 GCATGACCAA CAAGATGAAG AGCACCAAAG GCGCCCTGAC CTTCAGCCCC TACCTGCTGA 
     3661 GCCACGTGAT GGGCTACGGC TTCTACCACT TCGGCACCTA CCCCAGCGGC TACGAGAACC 
     3721 CCTTCCTGCA CGCCATCAAC AACGGCGGCT ACACCAACAC CCGCATCGAG AAGTACGAGG 
     3781 ACGGCGGCGT GCTGCACGTG AGCTTCAGCT ACCGCTACGA GGCCGGCCGC GTGATCGGCG 
     3841 ACTTCAAGGT GATGGGCACC GGCTTCCCCG AGGACAGCGT GATCTTCACC GACAAGATCA 
     3901 TCCGCAGCAA CGCCACCGTG GAGCACCTGC ACCCCATGGG CGATAACGAT CTGGATGGCA 
     3961 GCTTCACCCG CACCTTCAGC CTGCGCGACG GCGGCTACTA CAGCTCCGTG GTGGACAGCC 
     4021 ACATGCACTT CAAGAGCGCC ATCCACCCCA GCATCCTGCA GAACGGGGGC CCCATGTTCG 
     4081 CCTTCCGCCG CGTGGAGGAG GATCACAGCA ACACCGAGCT GGGCATCGTG GAGTACCAGC 
     4141 ACGCCTTCAA GACCCCGGAT GCAGATGCCG GTGAAGAAAG AGTTTAAACG GCCGGCCGCG 
     4201 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4261 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4321 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4381 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4441 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4501 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4561 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4621 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4681 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4741 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4801 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4861 CCAGCTTGGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     4921 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA 
     4981 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC 
     5041 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC 
     5101 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA 
     5161 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA 
     5221 GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT 
     5281 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT 
     5341 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG 
     5401 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC 
     5461 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC 
     5521 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC 
     5581 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG 
     5641 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG 
     5701 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT 
     5761 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG 
     5821 GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC 
     5881 TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCCG ACCGCTATCA GGACATAGCG 
     5941 TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG 
     6001 CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG 
     6061 TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT 
     6121 CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC 
     6181 GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC 
     6241 CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA 
     6301 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT 
     6361 CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC 
     6421 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA 
     6481 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT 
     6541 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC 
     6601 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC 
     6661 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 
     6721 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 
     6781 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 
     6841 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 
     6901 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 
     6961 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 
     7021 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 
     7081 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 
     7141 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 
     7201 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG 
     7261 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 
     7321 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 
     7381 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 
     7441 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 
     7501 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 
     7561 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC 
     7621 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC 
     7681 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT 
     7741 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG 
     7801 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA 
     7861 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA 
     7921 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT 
     7981 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG 
     8041 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA 
     8101 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC 
     8161 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT 
     8221 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC 
     8281 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA 
     8341 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA 
     8401 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA 
     8461 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 
     8521 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG 
     8581 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC 
     8641 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC 
     8701 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG 
     8761 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG 
     8821 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG 
     8881 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT 
     8941 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//