LOCUS       MG210582                8885 bp    DNA     circular  SYN 
DEFINITION  Tnfaip3 (tGFP-tagged) - Mouse tumor necrosis factor, alpha-induced protein 3 (Tnfaip3)
ACCESSION   NM_009397
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3366..4070 
                     /label="GFP"  
     misc_feature    4117..4702 
                     /label="PolyA signal"  
     misc_feature    6794..7439 
                     /label="ColE1"  
     misc_feature    5156..5950 
                     /label="Neo"  
     misc_feature    4787..5089 
                     /label="SV40 ori"  
     misc_feature    7454..8314 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3345..3351 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3345 
                     /label="Tnfaip3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGAACAA CTTCTTCCTC AGGCTTTGTA TTTGAGCAAT ATGCGGAAAG 
     1081 CTGTGAAGAT ACGAGAGAGA ACCCCAGAAG ACATTTTCAA ACCTACCAAT GGGATCATCT 
     1141 ATCACTTTAA AACCATGCAC CGATACACGC TGGAGATGTT CAGAACATGC CAGTTTTGCC 
     1201 CACAGTTCCG AGAGATCATC CACAAAGCAC TTATTGACAG AAGTGTCCAG GCTTCCCTGG 
     1261 AAAGCCAGAA GAAGCTCAAC TGGTGTCGTG AAGTCAGGAA GCTCGTGGCT CTGAAAACCA 
     1321 ATGGTGATGG AAACTGCCTC ATGCATGCAG CTTGTCAGTA CATGTGGGGT GTTCAGGATA 
     1381 CTGACCTGGT CCTGAGGAAG GCCCTCTGCA GCACCCTTAA GGAGACAGAC ACTCGGAACT 
     1441 TTAAATTCCG CTGGCAGCTG GAATCTCTGA AATCTCAGGA ATTTGTGGAA ACAGGACTTT 
     1501 GCTACGACAC TCGGAACTGG AATGACGAAT GGGACAACTT GGTCAAAATG GCATCAGCAG 
     1561 ACACACCTGC AGCCCGAAGT GGACTTCAGT ACAATTCCCT GGAAGAAATC CACATATTTG 
     1621 TCCTCAGCAA CATCCTCAGA AGACCCATCA TTGTCATTTC AGACAAAATG CTAAGAAGTT 
     1681 TGGAATCTGG TTCCAATTTT GCTCCTTTGA AAGTGGGTGG GATTTATCTG CCTCTTCACT 
     1741 GGCCTGCCCA GGAGTGTTAC AGATATCCCA TCGTCCTAGG CTATGACAGC CAGCACTTTG 
     1801 TACCCCTGGT GACCCTGAAG GACAGTGGAC CTGAACTTCG CGCTGTTCCA CTTGTTAACA 
     1861 GAGACCGGGG TAGGTTTGAA GACTTAAAAG TTCACTTCTT GACAGATCCT GAGAATGAGA 
     1921 TGAAGGAAAA GCTTCTAAAG GAGTACTTGA TAGTGATGGA GATCCCTGTG CAAGGCTGGG 
     1981 ACCACGGCAC GACTCACCTG ATCAACGCTG CAAAATTGGA TGAAGCTAAC TTACCCAAAG 
     2041 AAATAAATTT GGTAGACGAT TACTTTGAGC TTGTTCAGCA CGAATACAAG AAATGGCAGG 
     2101 AGAACAGCGA TCAGGCCAGG AGAGCGGCAC ATGCGCAGAA CCCCTTGGAG CCTTCCACAC 
     2161 CCCAGCTATC ACTCATGGAT ATAAAATGTG AGACACCCAA CTGTCCTTTC TTCATGTCCG 
     2221 TGAACACTCA GCCTTTATGC CACGAATGCT CAGAGAGGCG CCAAAAGAAT CAGAGCAAGC 
     2281 TCCCAAAGCT GAACTCGAAG CTAGGCCCTG AAGGACTCCC AGGCGTGGGA CTTGGCTCCT 
     2341 CAAACTGGAG CCCCGAGGAA ACCGCTGGAG GACCTCATTC AGCCCCACCC ACAGCACCCA 
     2401 GCCTTTTTCT CTTCAGTGAG ACCACTGCAA TGAAGTGCAG GAGTCCTGGG TGCCCTTTTA 
     2461 CTTTGAATGT GCAGCATAAT GGATTCTGTG AGCGTTGCCA CGCCCGGCAG ATTAATGCCA 
     2521 GCCACACCGC AGACCCTGGA AAGTGCCAAG CCTGCCTTCA GGATGTCACT CGGACCTTTA 
     2581 ATGGCATCTG CAGTACCTGT TTCAAAAGGA CTACAGCAGA GCCCAGCTCC AGCCTCACTT 
     2641 CCAGTATCCC TGCCTCCTGT CACCAACGCT CCAAGTCTGA CCCCTCACAA CTCATCCAAA 
     2701 GTCTCACTCC ACACTCTTGC CACCGGACTG GAAATGTCTC TCCTTCTGGC TGCCTCTCCC 
     2761 AGGCTGCACG GACTCCAGGA GACAGAGCAG GGACAAGCAA GTGCAGGAAA GCTGGCTGCA 
     2821 TGTATTTTGG GACTCCAGAA AACAAGGGCT TTTGCACTCT ATGTTTCATC GAATACAGAG 
     2881 AAAATAAGCA GTCTGTTACT GCCTCTGAGA AAGCTGGTTC CCCGGCCCCC AGGTTCCAGA 
     2941 ACAATGTCCC GTGCCTGGGC AGGGAGTGCG GCACACTCGG AAGCACCATG TTTGAAGGGT 
     3001 ACTGTCAGAA GTGTTTCATC GAAGCTCAGA ACCAGAGATT CCATGAAGCA AGAAGAACGG 
     3061 AAGAACAGCT GAGATCAAGC CAGCATAGAG ACATGCCTCG AACTACACAG GTAGCCTCAA 
     3121 GGCTGAAATG TGCCCGGGCC TCCTGCAAGA ACATTCTGGC CTGTCGCAGT GAGGAACTCT 
     3181 GTATGGAGTG CCAGCACCTA AGCCAACGAG TAGGTTCTGT GGCCCACCGG GGTGAGCCCA 
     3241 CGCCTGAAGA GCCCCCTAAA CAGCGCTGCC GGGCCCCTGC TTGTGATCAC TTTGGCAATG 
     3301 CCAAGTGTAA TGGTTACTGC AATGAGTGCT ACCAGTTCAA GCAGATGTAT GGCACGCGTA 
     3361 CGCGGCCGCT CGAGATGGAG AGCGACGAGA GCGGCCTGCC CGCCATGGAG ATCGAGTGCC 
     3421 GCATCACCGG CACCCTGAAC GGCGTGGAGT TCGAGCTGGT GGGCGGCGGA GAGGGCACCC 
     3481 CCGAGCAGGG CCGCATGACC AACAAGATGA AGAGCACCAA AGGCGCCCTG ACCTTCAGCC 
     3541 CCTACCTGCT GAGCCACGTG ATGGGCTACG GCTTCTACCA CTTCGGCACC TACCCCAGCG 
     3601 GCTACGAGAA CCCCTTCCTG CACGCCATCA ACAACGGCGG CTACACCAAC ACCCGCATCG 
     3661 AGAAGTACGA GGACGGCGGC GTGCTGCACG TGAGCTTCAG CTACCGCTAC GAGGCCGGCC 
     3721 GCGTGATCGG CGACTTCAAG GTGATGGGCA CCGGCTTCCC CGAGGACAGC GTGATCTTCA 
     3781 CCGACAAGAT CATCCGCAGC AACGCCACCG TGGAGCACCT GCACCCCATG GGCGATAACG 
     3841 ATCTGGATGG CAGCTTCACC CGCACCTTCA GCCTGCGCGA CGGCGGCTAC TACAGCTCCG 
     3901 TGGTGGACAG CCACATGCAC TTCAAGAGCG CCATCCACCC CAGCATCCTG CAGAACGGGG 
     3961 GCCCCATGTT CGCCTTCCGC CGCGTGGAGG AGGATCACAG CAACACCGAG CTGGGCATCG 
     4021 TGGAGTACCA GCACGCCTTC AAGACCCCGG ATGCAGATGC CGGTGAAGAA AGAGTTTAAA 
     4081 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4141 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4201 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4261 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4321 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4381 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4441 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4501 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4561 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4621 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4681 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4741 TTGGGTACGC GGCCAGCTTG GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA 
     4801 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 
     4861 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     4921 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 
     4981 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG 
     5041 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 
     5101 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 
     5161 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 
     5221 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 
     5281 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 
     5341 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 
     5401 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 
     5461 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 
     5521 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 
     5581 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 
     5641 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 
     5701 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 
     5761 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC CGACCGCTAT 
     5821 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 
     5881 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 
     5941 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 
     6001 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 
     6061 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 
     6121 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 
     6181 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 
     6241 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 
     6301 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 
     6361 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 
     6421 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 
     6481 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 
     6541 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 
     6601 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 
     6661 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 
     6721 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 
     6781 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 
     6841 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 
     6901 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 
     6961 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 
     7021 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 
     7081 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 
     7141 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 
     7201 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC 
     7261 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 
     7321 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 
     7381 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 
     7441 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 
     7501 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 
     7561 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 
     7621 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 
     7681 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 
     7741 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 
     7801 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 
     7861 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 
     7921 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 
     7981 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 
     8041 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 
     8101 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 
     8161 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 
     8221 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 
     8281 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 
     8341 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 
     8401 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC 
     8461 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC 
     8521 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG 
     8581 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT 
     8641 TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC 
     8701 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT 
     8761 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA 
     8821 TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA 
     8881 ATTTT
//