LOCUS       MG210577                8885 bp    DNA     circular  SYN 
DEFINITION  Ecel1 (tGFP-tagged) - Mouse endothelin converting enzyme-like 1 (Ecel1)
ACCESSION   NM_021306
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3366..4070 
                     /label="GFP"  
     misc_feature    4117..4702 
                     /label="PolyA signal"  
     misc_feature    6794..7439 
                     /label="ColE1"  
     misc_feature    5156..5950 
                     /label="Neo"  
     misc_feature    4787..5089 
                     /label="SV40 ori"  
     misc_feature    7454..8314 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3345..3351 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3345 
                     /label="Ecel1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCCCCG TATTCCATGA CAGCGCACTA CGACGAGTTC CAGGAAGTCA 
     1081 AGTATGTGAG CCGGTGTGGC ACCGGGGGCG CTCGAGGGAC CTCACTGCCT CCAGGCTTCC 
     1141 CGAGGGGCTC AGGGCGCAGT GCTAGCGGGT CCAGGTCTGG GCTGCCCCGC TGGAATCGAC 
     1201 GCGAGGTGTG CCTGCTGTCG GGGTTGGTGT TTGCTGCCGG CCTCTGCGCC ATCCTGGCGG 
     1261 CCATGCTGGC GCTCAAATAC CTGGGCCCGG GAGCGGCGGG CGGTGGCGGA GCCTGTCCCG 
     1321 AGGGCTGCCC CGAGCGCAAG GCCTTTGCAC GCGCCGCCCG CTTTCTGTCT GCCAACCTGG 
     1381 ACGCCAGCAT CGACCCGTGC CAGGACTTCT ACTCGTTCGC GTGCGGAGGC TGGCTGCGGC 
     1441 GCCACGCTAT CCCCGACGAC AAGCTCACCT ACGGCACGAT CGCGGCCATC GGAGAGCAGA 
     1501 ACGAGGAGCG TCTGCGGCGC CTGCTGGCGA GACCCACCGG AGGCCCCGGC GGCGCTGCGC 
     1561 AGCGCAAGGT GCGCGCCTTC TTCCGCTCGT GCCTCGACAT GCGCGAGATC GAGAGGCTCG 
     1621 GGCCGCGGCC CATGCTCGAG GTCATCGAGG ACTGCGGCGG CTGGGACCTG GGCGGCGCGG 
     1681 CCGATCGCCC CGGGGCGGCT CGCTGGGACC TCAACCGACT GCTGTACAAG GCACAGGGTG 
     1741 TGTACAGCGC GGCCGCGCTC TTCTCGCTTA CAGTCAGCCT GGACGACAGG AACTCCTCGC 
     1801 GCTACGTCAT CCGCATTGAC CAGGATGGGC TCACCCTGCC AGAGAGAACC CTGTACCTAG 
     1861 CTCAAGACGA GGAGAGTGAG AAGATCTTGG CTGCATACAG GGTGTTCATG CAGCGTCTAC 
     1921 TCAGACTACT GGGAGCGGAC GCTGTGGAGC AGAAGGCGCA GGAAATCCTT CAGCTGGAAC 
     1981 AGAGGCTGGC CAATATCTCT GTGTCAGAAT ATGATGATCT CCGTCGTGAT GTCAGCTCCG 
     2041 CGTACAACAA AGTGACTCTG GGGCAGCTGC AGAAGATCAT CCCCCATTTG CAGTGGAAGT 
     2101 GGCTGCTGGA TCAGATCTTC CAGGAAGACT TCTCGGAGGA GGAGGAGGTG GTGCTGCTGG 
     2161 CCACAGACTA CATGCAGCAG GTGTCCCAGC TCATCCGCTC CACACCTCGA AGGATCCTGC 
     2221 ACAACTACCT AGTGTGGCGT GTGGTGGTGG TTCTGAGTGA GCACCTGTCC TCACCATTCC 
     2281 GAGAGGCACT GCATGAGCTG GCCAAAGAGA TGGAGGGCAA TGACAAGCCC CAGGAGTTGG 
     2341 CCCGAGTCTG CCTGGGCCAG GCCAACCGCC ACTTCGGCAT GGCTCTTGGT GCCCTCTTTG 
     2401 TACATGAACA CTTCTCAGCT GCCAGTAAAG CCAAGGTGCA GCAGCTAGTG GAAGATATCA 
     2461 AGTACATCTT GGGCCAGCGC CTGGAGGAGC TGGACTGGAT GGATGCCCAG ACCAAGGCAG 
     2521 CTGCTCGGGC CAAGCTCCAG TACATGATGG TCATGGTTGG CTATCCAGAC TTCCTGCTGA 
     2581 AACCTGAGGC TGTAGACAAA GAATATGAGT TCGAGGTCCA CGAGAAGACC TACTTCAAGA 
     2641 ACATTTTGAA CAGCATCCGA TTCAGTATCC AGTTGTCCGT CAAAAAGATT CGACAGGAGG 
     2701 TGGACAAATC CTCGTGGCTC CTCCCACCAC AGGCGCTCAA TGCCTACTAT CTGCCCAACA 
     2761 AGAATCAAAT GGTCTTCCCA GCTGGCATCC TGCAGCCCAC CCTGTATGAC CCAGACTTCC 
     2821 CACAGTCTCT GAACTACGGG GGTATTGGCA CCATCATTGG GCACGAACTG ACCCATGGCT 
     2881 ATGACGACTG GGGGGGACAG TATGACCGCT CAGGAAACCT GTTGCACTGG TGGACTGAGA 
     2941 CCTCCTACAG CCACTTTCTG CGCAAGGCCG AGTGCATCGT TCGCCTCTAT GACAACTTCA 
     3001 CCGTCTACAA CCAGCGCGTG AATGGGAAAC ACACACTTGG CGAGAACATC GCAGACATGG 
     3061 GAGGCCTCAA GCTGGCCTAC TATGCCTATC AGAAGTGGGT CCGGGAGCAT GGCCCGGAGC 
     3121 ACCCGCTGCA CCGGCTCAAG TACACACACA ACCAGCTTTT CTTCATTGCC TTTGCACAGA 
     3181 ACTGGTGCAT CAAGCGACGG TCACAGTCCA TCTACCTGCA GGTGCTGACA GACAAGCATG 
     3241 CACCTGAGCA CTACCGGGTC CTGGGCAGCG TATCCCAGTT TGAGGAATTC GGCCGGGCCT 
     3301 TCCACTGTCC CAAGGACTCT CCCATGAACC CCGTCCATAA GTGCTCTGTG TGGACGCGTA 
     3361 CGCGGCCGCT CGAGATGGAG AGCGACGAGA GCGGCCTGCC CGCCATGGAG ATCGAGTGCC 
     3421 GCATCACCGG CACCCTGAAC GGCGTGGAGT TCGAGCTGGT GGGCGGCGGA GAGGGCACCC 
     3481 CCGAGCAGGG CCGCATGACC AACAAGATGA AGAGCACCAA AGGCGCCCTG ACCTTCAGCC 
     3541 CCTACCTGCT GAGCCACGTG ATGGGCTACG GCTTCTACCA CTTCGGCACC TACCCCAGCG 
     3601 GCTACGAGAA CCCCTTCCTG CACGCCATCA ACAACGGCGG CTACACCAAC ACCCGCATCG 
     3661 AGAAGTACGA GGACGGCGGC GTGCTGCACG TGAGCTTCAG CTACCGCTAC GAGGCCGGCC 
     3721 GCGTGATCGG CGACTTCAAG GTGATGGGCA CCGGCTTCCC CGAGGACAGC GTGATCTTCA 
     3781 CCGACAAGAT CATCCGCAGC AACGCCACCG TGGAGCACCT GCACCCCATG GGCGATAACG 
     3841 ATCTGGATGG CAGCTTCACC CGCACCTTCA GCCTGCGCGA CGGCGGCTAC TACAGCTCCG 
     3901 TGGTGGACAG CCACATGCAC TTCAAGAGCG CCATCCACCC CAGCATCCTG CAGAACGGGG 
     3961 GCCCCATGTT CGCCTTCCGC CGCGTGGAGG AGGATCACAG CAACACCGAG CTGGGCATCG 
     4021 TGGAGTACCA GCACGCCTTC AAGACCCCGG ATGCAGATGC CGGTGAAGAA AGAGTTTAAA 
     4081 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4141 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4201 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4261 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4321 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4381 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4441 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4501 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4561 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4621 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4681 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4741 TTGGGTACGC GGCCAGCTTG GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA 
     4801 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 
     4861 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     4921 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 
     4981 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG 
     5041 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 
     5101 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 
     5161 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 
     5221 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 
     5281 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 
     5341 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 
     5401 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 
     5461 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 
     5521 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 
     5581 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 
     5641 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 
     5701 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 
     5761 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC CGACCGCTAT 
     5821 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 
     5881 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 
     5941 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 
     6001 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 
     6061 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 
     6121 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 
     6181 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 
     6241 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 
     6301 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 
     6361 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 
     6421 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 
     6481 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 
     6541 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 
     6601 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 
     6661 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 
     6721 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 
     6781 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 
     6841 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 
     6901 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 
     6961 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 
     7021 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 
     7081 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 
     7141 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 
     7201 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC 
     7261 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 
     7321 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 
     7381 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 
     7441 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 
     7501 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 
     7561 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 
     7621 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 
     7681 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 
     7741 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 
     7801 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 
     7861 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 
     7921 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 
     7981 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 
     8041 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 
     8101 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 
     8161 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 
     8221 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 
     8281 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 
     8341 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 
     8401 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC 
     8461 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC 
     8521 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG 
     8581 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT 
     8641 TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC 
     8701 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT 
     8761 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA 
     8821 TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA 
     8881 ATTTT
//