LOCUS       MG209724                8498 bp    DNA     circular  SYN 
DEFINITION  Wee1 (tGFP-tagged) - Mouse wee 1 homolog (S. pombe) (Wee1)
ACCESSION   NM_009516
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2979..3683 
                     /label="GFP"  
     misc_feature    3730..4315 
                     /label="PolyA signal"  
     misc_feature    6407..7052 
                     /label="ColE1"  
     misc_feature    4769..5563 
                     /label="Neo"  
     misc_feature    4400..4702 
                     /label="SV40 ori"  
     misc_feature    7067..7927 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2958..2964 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2958 
                     /label="Wee1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCTTCCTG AGCCGACAGC AGCCGCCGCC CACCCGCCGC GTAGGAGCCG 
     1081 CTTACAGTCT GCGGCAGAAG CTCATCTTCT CGCCGGGCAG CGACTGTGAG GAAGAGGAGG 
     1141 AGGAGGAGGA GGAAGGCAGC GGCCACAGTA CGGGGGAGGA CTCGGCCTTC CAGGAACCGG 
     1201 ATTCGCCCCT GCCTTCTGCA CGCAGCCCTG CCGAGGCCGA GGCCGAGCGC CGCCGTCGCT 
     1261 CGCCCGGCGC GGAGCCCAGC AGCCCGGGAG AGCTGGAGGA CGACTTGCTG CTGCAGGGCG 
     1321 GCGGCGGAGG CGCGCAAGCG GCGGGTGGAG GCGCCGAGGG CGACTCTTGG GAGGAGGAGG 
     1381 GTTTCGGCTC CTCGTCGCCC GTCAAATCAC CTTCGACCGC CTACTTTCTG AGCAGCCCGT 
     1441 TCTCGCCAGT GCGCTGCGGC GGCCCAGGGG ATGCCTCCCC GCAGGGTTGC GGGGCTCCGC 
     1501 GAGCCATGGA CGACCCCTGC TCGCCGCAGC CCGACTACCC GAGCACCCCT CCGCACAAGA 
     1561 CCTTCCGCAA GCTGCGCCTG TTCGACACGC CGCACACTCC CAAGAGTTTG CTTTCCAAAG 
     1621 CGCGAGTTAT TGATTCCGGC TCTGTTAAAC TCCGGGGTAG TTCTCTATTC ATGGACACAG 
     1681 AAAAGTCAGG AAAAAGAGAA TTCGATACAC GGCAAACTCC CCAAGTGAAT ATTAACCCCT 
     1741 TTACTCCGGA TCCTGTACTG CTCCATTCCT CAGGACGGTG TCGTGGGAGA AAGAGAGCGT 
     1801 ATTTTAATGA TTCCTCTGAA GACATGGAAG CCAGTGATTA TGAGTTTGAA GATGAAACAA 
     1861 GACCTGCCAA AAGAATTACA ATTACTGAAA GCAACATGAA GTCACGGTAT ACAACTGAAT 
     1921 TTCATGAGCT GGAGAAAATT GGTTCTGGAG AATTTGGTTC TGTGTTTAAA TGTGTGAAGA 
     1981 GGTTAGATGG ATGCATTTAT GCCATTAAAC GATCAAAAAA ACCATTGGCT GGCTCTGTTG 
     2041 ATGAGCAGAA TGCTTTGAGA GAAGTGTATG CTCACGCTGT GCTTGGACAG CACCCCCACG 
     2101 TCGTTCGCTA TTTCTCTGCC TGGGCAGAGG ATGACCACAT GCTTATACAG AACGAATACT 
     2161 GTAATGGTGG GAGTTTAGCT GATGCTATAA GTGAGAACTA CAGAGTCATG AGCTACTTGA 
     2221 CTGAAGTAGA GCTGAAGGAT CTCCTTTTGC AAGTTGGCCG GGGCTTGAGA TACATACATT 
     2281 CAATGTCTTT GGTTCACATG GATATAAAAC CTAGTAATAT TTTTATATCT CGAACCTCAA 
     2341 TCCCAAATGC TGTCTCTGAG GAAGGAGATG AAGATGACTG GATATCCAAT AAAGTTATGT 
     2401 TTAAAATAGG TGATCTTGGG CATGTAACAA GAATCTCTAG TCCTCAAGTT GAAGAAGGTG 
     2461 ATAGTCGTTT TCTAGCAAAT GAAGTTTTAC AAGAGAACTA TAGCCATCTA CCGAAAGCAG 
     2521 ATATTTTTGC TCTTGCTCTC ACAGTCGTAT GTGCTGCTGG TGCTGAACCC CTTCCCAGAA 
     2581 ATGGAGAGCA GTGGCACGAG ATCCGGCAGG GTCGGTTACC TCGGATCCCA CAAGTGCTTT 
     2641 CCCAGGAGGT GACAGAGTTG CTGAGAGTCA TGATTCATCC TGACCCAGAG AGAAGGCCTT 
     2701 CAGCAATGGA GCTGGTGAAG CATTCAGTGT TGTTGTCTGC ATCTAGAAAG AGCGCAGAGC 
     2761 AGTTACGAAT AGAATTGAAT GCTGAAAAAT TCAAAAATTC TCTTTTGCAG AAAGAACTCA 
     2821 AGAAAGCCCA AATGGCAGCC AAAGTTGCAG CTGAGGAACG GGCACTCTTC ACAGATCGGA 
     2881 TGGCCACTAG ATCCACCACC CAGAGTAATA GAACTTCTCG ACTTATTGGA AAGAAAATGA 
     2941 ACCGCTCTGT CAGCCTTACT ATATACACGC GTACGCGGCC GCTCGAGATG GAGAGCGACG 
     3001 AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG 
     3061 AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCGAGCA GGGCCGCATG ACCAACAAGA 
     3121 TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT 
     3181 ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA 
     3241 TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC 
     3301 ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGATGG 
     3361 GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA 
     3421 CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGATCTGGA TGGCAGCTTC ACCCGCACCT 
     3481 TCAGCCTGCG CGACGGCGGC TACTACAGCT CCGTGGTGGA CAGCCACATG CACTTCAAGA 
     3541 GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG 
     3601 AGGAGGATCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC 
     3661 CGGATGCAGA TGCCGGTGAA GAAAGAGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3721 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3781 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3841 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3901 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3961 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4021 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4081 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4141 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4201 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4261 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4321 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC TTGGCTGTGG 
     4381 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     4441 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     4501 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 
     4561 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 
     4621 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 
     4681 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 
     4741 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 
     4801 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 
     4861 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 
     4921 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 
     4981 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 
     5041 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 
     5101 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 
     5161 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 
     5221 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 
     5281 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 
     5341 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 
     5401 GACTGTGGCC GGCTGGGTGT GGCCGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 
     5461 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 
     5521 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 
     5581 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 
     5641 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 
     5701 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 
     5761 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 
     5821 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 
     5881 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 
     5941 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 
     6001 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 
     6061 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 
     6121 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 
     6181 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 
     6241 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 
     6301 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 
     6361 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 
     6421 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 
     6481 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 
     6541 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 
     6601 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 
     6661 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 
     6721 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 
     6781 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 
     6841 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 
     6901 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 
     6961 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 
     7021 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 
     7081 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 
     7141 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 
     7201 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 
     7261 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 
     7321 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 
     7381 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 
     7441 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 
     7501 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 
     7561 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 
     7621 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 
     7681 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 
     7741 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 
     7801 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 
     7861 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 
     7921 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 
     7981 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 
     8041 GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 
     8101 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 
     8161 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 
     8221 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 
     8281 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 
     8341 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 
     8401 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 
     8461 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//