LOCUS       MG209493                8429 bp    DNA     circular  SYN 
DEFINITION  Cdc16 (tGFP-tagged) - Mouse CDC16 cell division cycle 16 homolog (S. cerevisiae) (Cdc16)
ACCESSION   NM_027276
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2908..3612 
                     /label="GFP"  
     misc_feature    3659..4244 
                     /label="PolyA signal"  
     misc_feature    6336..6981 
                     /label="ColE1"  
     misc_feature    4698..5492 
                     /label="Neo"  
     misc_feature    4329..4631 
                     /label="SV40 ori"  
     misc_feature    6996..7856 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2880..2887 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2880 
                     /label="Cdc16"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACCTGGAG CCGCTGCGCA AACGCGTCCG GCAGTACCTG GACCAGCAAC 
     1081 AGTATCAAAG TGCTCTGTTT TGGGCAGACA AGGTAGCTTC ACTCTCTCAT GAGGAGCCCC 
     1141 AGGATGTTTA TTGGTTGGCT CAGTGCCTTT ACCTCACAGC GCAGTACCAC CGAGCAGCCC 
     1201 ATGCGCTCCG GTCACGCAAG CTGGACAAAT TGTATGAAGC TTGCCGATAC CTTGCAGCTA 
     1261 GATGCCACTA TGCTGCAAAA GAGCACCAGC AGGCTCTGGA CATCCTTGAC ATGGAGGAGC 
     1321 CAATTAACAG AAGGCTCTTT GAGAAATACC TGAAGGATGA CAATGGCTCG AGAGACCCTT 
     1381 CCAGTGACTG GGAGATGTCA CAGTCTTCCA TAAAGAGCTC GATTTGTCTC CTGAGAGGGA 
     1441 AGATCTATGA TGCTTTAGAT AATCGCACCC TGGCTACCTA CAGTTACAAA GAGGCTCTGA 
     1501 AACTGGATGT CTACTGCTTT GAAGCCTTTG ACCTTTTGAC ATCACACCAC ATGCTGACAG 
     1561 CACAGGAAGA AAAAGAACTT CTGGACTCCC TGCCTCTTAA CAAGCTCTGT GCTGAAGAAC 
     1621 AAGAGTTGCT GCGATTTGTA TTTGAGAACA AATTAAAGAA GTATAATAAG CCCAGTGAAA 
     1681 CGGTCATTCC CGAGTCAGTG GACGGCTTGC AGGAGAATCT GGACGTGGTA GTGTCTTTAG 
     1741 CTGAGAGACA CTATTACAAC TGTGATTTTA AGATGTGCTA CAAGCTTACT TCTACAGTGA 
     1801 TGGAGAAAGA TCCTTTCCAT GCAAACTGTT TACCTGTGCA CATAGGAACT CTTGTGGAGC 
     1861 TGAATAAAGC GAATGAACTT TTCTATCTTT CTCACAAACT GGTGGATTTA TATCCGAGTA 
     1921 ATCCTGTGTC TTGGTTTGCG GTGGGATGTT ACTATCTCAT GGTTGGTCAT AAAAATGAGC 
     1981 ATGCCAGAAG ATATCTCAGC AAAGCTACCA CGTTGGAGAA GACCTACGGT CCTGCCTGGA 
     2041 TAGCCTACGG GCATTCCTTC GCTGTGGAGA GTGAGCACGA CCAAGCAATG GCTGCTTACT 
     2101 TCACAGCAGC ACAGCTGATG AAGGGGTGTC ACTTGCCAAT GCTATATATT GGATTAGAAT 
     2161 ATGGTCTGAC CAATAACTCG AAACTGGCTG AGCGGTTCTT TGGCCAAGCT CTGAGCATCG 
     2221 CCCCAGAAGA CCCATTTGTC ATCCATGAGG TTGGAGTGGT TGCCTTTCAG AATGGAGAAT 
     2281 GGAAAACAGC AGAAAAATGG TTTCTCGATG CTTTGGAAAA AATTAAAGCA ATTGGAAATG 
     2341 AGGTCACAGT TGACAAATGG GAACCTTTGT TGAACAACTT GGGGCATGTC TGCAGAAAAC 
     2401 TTAAGAAATA TGCTGAGGCT TTGGATTACC ACAGGCAGGC ACTGGTGCTA ATCCCTCAGA 
     2461 ACGCATCCAC CTACTCTGCT ATAGGGTACA TCCACAGTCT GATGGGCAAC TTTGAAAACG 
     2521 CTGTGGACTA TTTCCACACA GCTCTTGGTC TCAGGCGAGA CGACACATTT TCTGTTACAA 
     2581 TGCTTGGTCA CTGCATTGAA ATGTACATCG GGGATTCGGA AGCTTATATC GGAGCAGACA 
     2641 TTAAAGACAA ATTAAAGTGC TATGACTTCG ATGTGCATAC AATGAAAACA CTAAAAAACA 
     2701 TCATTTCGCC TCCATGGGAT TTCAGGGACT TTGAAGTAGA AAAGCAGAAC ACAGAGGAAG 
     2761 CGGGACTTGC ACCTCTGCAA AACTCCACTA AAGCTCCAGA GTCCAGACCT AACTTGGAAG 
     2821 AGACTTTTGA AATTGAAATG AACGAAAGTG ACATGATGCT AGAGACGTCT ATGTCAGACC 
     2881 ACAGCACAAG CGGACCGACG CGTACGCGGC CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC 
     2941 TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC 
     3001 TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA 
     3061 CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT 
     3121 ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG 
     3181 GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT 
     3241 TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGATG GGCACCGGCT 
     3301 TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC 
     3361 ACCTGCACCC CATGGGCGAT AACGATCTGG ATGGCAGCTT CACCCGCACC TTCAGCCTGC 
     3421 GCGACGGCGG CTACTACAGC TCCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC 
     3481 ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGGATC 
     3541 ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCGGATGCAG 
     3601 ATGCCGGTGA AGAAAGAGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3661 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3721 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     3781 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     3841 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     3901 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     3961 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4021 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4081 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4141 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4201 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4261 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CTTGGCTGTG GAATGTGTGT 
     4321 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     4381 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     4441 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 
     4501 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 
     4561 TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 
     4621 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 
     4681 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 
     4741 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 
     4801 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 
     4861 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 
     4921 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 
     4981 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 
     5041 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 
     5101 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 
     5161 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 
     5221 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 
     5281 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 
     5341 CGGCTGGGTG TGGCCGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 
     5401 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 
     5461 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 
     5521 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 
     5581 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC 
     5641 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA 
     5701 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 
     5761 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA 
     5821 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 
     5881 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 
     5941 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 
     6001 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 
     6061 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 
     6121 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 
     6181 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 
     6241 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 
     6301 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 
     6361 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 
     6421 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 
     6481 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 
     6541 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 
     6601 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 
     6661 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 
     6721 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 
     6781 GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 
     6841 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 
     6901 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 
     6961 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 
     7021 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 
     7081 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 
     7141 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 
     7201 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 
     7261 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 
     7321 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 
     7381 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 
     7441 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 
     7501 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 
     7561 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 
     7621 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 
     7681 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 
     7741 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 
     7801 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 
     7861 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 
     7921 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 
     7981 CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 
     8041 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT 
     8101 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC 
     8161 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG 
     8221 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT 
     8281 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG 
     8341 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC 
     8401 TGATTTAACA AAAATTTAAC GCGAATTTT
//