LOCUS       MG209421                9377 bp    DNA     circular  SYN 
DEFINITION  Trak1 (tGFP-tagged) - Mouse trafficking protein, kinesin binding 1 (Trak1)
ACCESSION   NM_175114
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3858..4562 
                     /label="GFP"  
     misc_feature    4609..5194 
                     /label="PolyA signal"  
     misc_feature    7286..7931 
                     /label="ColE1"  
     misc_feature    5648..6442 
                     /label="Neo"  
     misc_feature    5279..5581 
                     /label="SV40 ori"  
     misc_feature    7946..8806 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3837..3843 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3837 
                     /label="Trak1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCATTGGCA ATTCAGCTCA GGCAGCCCTC CAGGGCCCAA CCTCTGCCAG 
     1081 GACTCAGCCA CACACTGGCT GGGACAGACT CATGTGACGT GTGCAACAGC ACCAACCTTC 
     1141 CAGAAGTTGA GATCATTAGC CTGCTGGAGG AACAGCTGCC CCATTACAAG CTAAGAGCGG 
     1201 ACACCATCTA TGGTTACGAC CACGATGACT GGCTGCATAC GCCCCTCATC TCCCCAGATG 
     1261 CCAACATCGA CCTCACAACT GAGCAGATCG AAGAGACGCT GAAATACTTC CTTCTATGTG 
     1321 CCGAAAGAGT TGGCCAGATG ACTAAGACAT ACAATGACAT TGATGCTGTC ACGAGGCTTC 
     1381 TTGAGGAGAA AGAGCGGGAT TTGGAGCTGG CTGCGAGGAT CGGTCAGTCA TTGTTGAAGA 
     1441 AGAACAAGAC CCTAACTGAG AGGAATGAAC TGCTGGAAGA GCAGGTGGAG CACATCCGGG 
     1501 AGGAGGTGTC TCAGCTCCGA CATGAGCTGT CCATGAAAGA CGAGCTGCTT CAATTCTACA 
     1561 CCAGTGCCGC TGAGGAGAGC GAGCCTGAGT CCGTCTGCTC AACCCCGCTG AAGAGGAACG 
     1621 AGTCCTCCTC CTCCGTCCAG AATTACTTCC ACCTGGACTC TCTTCAGAAG AAGCTCAAGG 
     1681 ACCTCGAAGA GGAGAACGTT GTACTTCGAT CCGAGGCCTG TCAGCTGAAG ACAGAGACCA 
     1741 TCACCTATGA GGAGAAGGAG CAGCAGCTGG TGAACGACTG CGTGAAGGAG CTGAGGGATG 
     1801 CCAACGTCCA GATTGCGAGC ATCTCTGAGG AGCTGGCTAA GAAGACGGAA GATGCCGCCC 
     1861 GCCAGCAGGA GGAGATCACC CACCTGCTGT CGCAAATCGT GGACTTACAG AAGAAGGCAA 
     1921 AATCTTGCGC GGTGGAAAAT GAAGAGCTTG TCCAGCACCT GGGCGCTGCC AAGGACGCCC 
     1981 AGCGTCAACT CACAGCCGAG CTCCGAGAGC TGGAGGATAA GTACGCGGAG TGCATGGAGA 
     2041 TGCTTCATGA GGCTCAGGAG GAGTTGAAGA ACCTGCGGAA CAAGACGATG CCCACGTCCC 
     2101 GGCGCTACCA CTCTCTGGGC CTGTTCCCCA TGGACTCGTT GGCTGCAGAA ATCGAGGGAA 
     2161 CTATGCGCAA GGAGCTGCAG CTGGAAGAGC TGGAGTCTCC AGACATCACC CACCAGAAGC 
     2221 GTGTCTTCGA GACGGTGAGG AACGTCAATC AAGTGGTCAA GCAGAGATCA CTAACCCCTT 
     2281 CTCCCATGAA CATCCCGGGC TCCAACCAGT CCTCAGCCAT GAACTCCCTC CTGTCCAGCT 
     2341 GCGTCAGCAC CCCTCGCTCC AGCTTCTACG GCAGCGACGT CAGCAACGTG GTCCTCGATA 
     2401 ACAAAACCAA CAGCATCCTC CTGGAGACCG AGGCAGCCGA CCTGGGGAAC GAGGACCACA 
     2461 ATAAGAAGCC GGGCACTCCA GGCACGCCAG GCTCCCATGA CCTGGAGACG GCACTTAGGC 
     2521 GGCTATCCCT ACGCCGGGAG AACTACCTGT CTGAGCGGAG GTTCTTCGAG GAGGAGCAGG 
     2581 AGAGGAAGCT GCGAGAGCTG GCTGAGAAGG GGGAGCTGCA CAGTGGCTCG CTCACGCCCA 
     2641 CCGAGAGCAT CATGTCCCTG GGAACACACT CACGCTTCTC CGAGTTCACG GGCTTCTCTG 
     2701 GCATGTCCTT CAGCAGCCGC TCCTACCTGC CCGAGAAGCT GCAGATCGTG AAGCCACTCG 
     2761 AAGGTTCAGC CACGCTTCAC CACTGGCAGC AATTGGCCCA GCCTCATCTT GGGGGCATCC 
     2821 TGGACCCCCG TCCTGGTGTG GTCACCAAGG GCTTCCGGAC TTTGGATGTT GACCTGGATG 
     2881 AAGTGTACTG CCTTAACGAC TTTGAGGAAG ATGACACAGG TGACCACATT TCTCTGGCGG 
     2941 GCCTAGCTAC CTCCACGCCA ATTCAGCACC CGGAGACCTC AGCGCACCAC CCTGGGAAGT 
     3001 GCATGTCACA GACCAACTCA ACTTTCACCT TTACCACTTG CCGCATCCTG CACCCTTCAG 
     3061 ACGAGCTCAC TCGGGTCACG CCAAGCCTTA ACTCTGCCCC AGCTCCGGCT TGTAGCAGTA 
     3121 CCAGCCACTT GAAATCCACA CCAGTGGCCA CGCCATGCAC CCCCCGGAGA CTGAGCCTGG 
     3181 CTGAGTCTTT CACAAATGTC CGTGAGTCCA CGACAACCAT GAGCACGTCC CTGGGGCTGG 
     3241 TATGGTTGCT GAAGGAGCGA GGCATCTCGG CGGCCGTGTA CGACCCCCAG AGTTGGGACA 
     3301 GAGCCGGCAG GGGCTCCCTC CTCCACTCCT ACACCCCTAG GATGGCTGTG ATCCCGTCCA 
     3361 CCCCACCAAA CTCCCCTATG CAGACACCTT CGGCCTCCCC GCCCTCCTTC GAGTTCAAGT 
     3421 GCACGAGCCC TCCCTACAAC AACTTCCTGG CTTCCAAGCC GGCCAGCTCT ATCCTGAGGG 
     3481 AGGTGAGGGA AAAGAGGCCC GTGCGGAGCA GCGAGAGCCA AACAGATGTG TCTGTCTCCA 
     3541 ACCTCAACCT TGTGGACAAA GTCAGGAGGT TTGGGGTGGC CAGAGTGGTG AACTCAGGAC 
     3601 GTGCCCGTAT CCCCACCTTG ACTGAGGAAC AGGGACCTCT CCTCTGTGGA CCCACAGGGC 
     3661 CAGCACAGGC TCTTGTCCCT GGGGGCCTGG TCCCCGAGGG CCTGCCTCTG GGGTGCCCCA 
     3721 GTGGCATCCG ACGGAATCGC AGCTTCCCCA CTATGGTCGG GTCCAGCGTG CAGATGAGAG 
     3781 CCCCCGTGAT CCTCACCTCG GGCATCTTGA TGGGTGCTAA GCTCCCCAAA CAGACTAGCT 
     3841 TGCGGACGCG TACGCGGCCG CTCGAGATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG 
     3901 AGATCGAGTG CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG 
     3961 GAGAGGGCAC CCCCGAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC 
     4021 TGACCTTCAG CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA 
     4081 CCTACCCCAG CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA 
     4141 ACACCCGCAT CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT 
     4201 ACGAGGCCGG CCGCGTGATC GGCGACTTCA AGGTGATGGG CACCGGCTTC CCCGAGGACA 
     4261 GCGTGATCTT CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA 
     4321 TGGGCGATAA CGATCTGGAT GGCAGCTTCA CCCGCACCTT CAGCCTGCGC GACGGCGGCT 
     4381 ACTACAGCTC CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC 
     4441 TGCAGAACGG GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGGATCAC AGCAACACCG 
     4501 AGCTGGGCAT CGTGGAGTAC CAGCACGCCT TCAAGACCCC GGATGCAGAT GCCGGTGAAG 
     4561 AAAGAGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4621 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4681 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4741 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4801 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4861 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4921 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4981 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5041 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5101 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5161 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5221 ATGCCTGTTG AATTGGGTAC GCGGCCAGCT TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT 
     5281 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5341 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5401 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 
     5461 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 
     5521 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 
     5581 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 
     5641 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 
     5701 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 
     5761 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 
     5821 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 
     5881 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 
     5941 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 
     6001 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 
     6061 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 
     6121 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 
     6181 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 
     6241 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 
     6301 GCCGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 
     6361 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 
     6421 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG 
     6481 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA 
     6541 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 
     6601 TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 
     6661 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 
     6721 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA 
     6781 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 
     6841 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 
     6901 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 
     6961 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 
     7021 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 
     7081 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 
     7141 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 
     7201 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 
     7261 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 
     7321 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 
     7381 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 
     7441 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 
     7501 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 
     7561 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 
     7621 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 
     7681 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT 
     7741 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 
     7801 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 
     7861 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 
     7921 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 
     7981 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 
     8041 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 
     8101 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 
     8161 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 
     8221 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 
     8281 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 
     8341 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 
     8401 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 
     8461 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 
     8521 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 
     8581 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 
     8641 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 
     8701 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 
     8761 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 
     8821 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 
     8881 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 
     8941 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 
     9001 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 
     9061 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 
     9121 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 
     9181 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 
     9241 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 
     9301 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 
     9361 AATTTAACGC GAATTTT
//